*2023/04/27 12:46:47.60 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2022/07/20 20:30:51.000 NUMBER OF RECORDS : 2 DATA DESCRIPTION : Bottle:Wire FILE TYPE : ASCII NUMBER OF CHANNELS : 13 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- -------- -------------- -------------- 1 Depth:Nominal metres 0 392 2 Sample_Number n/a 1 2 3 Chlorophyll:Extracted mg/m^3 1.4 1.4 4 Flag:Chlorophyll:Extracted n/a 5 Phaeo-Pigment:Extracted mg/m^3 0.29 0.29 6 Salinity:Bottle PSS-78 15.6453 30.9453 7 Flag:Salinity:Bottle n/a 8 Nitrate_plus_Nitrite umol/L 0.17 30.14 9 Flag:Nitrate_plus_Nitrite n/a 10 Silicate umol/L 45.1 67.27 11 Flag:Silicate n/a 12 Phosphate umol/L 0.111 2.863 13 Flag:Phosphate n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 6 F ' ' 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F R4 2 4 ' ' ' ' 3 NQ C ' ' 5 -99 ' ' 7 F R4 2 6 -99 ' ' 9 F R4 4 7 ' ' ' ' 3 NQ C ' ' 8 -99 ' ' 7 F R4 2 9 ' ' ' ' 3 NQ C ' ' 10 -99 ' ' 7 F R4 2 11 ' ' ' ' 3 NQ C ' ' 12 -99 ' ' 8 F R4 3 13 ' ' ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2022-029 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : GEO1 EVENT NUMBER : 1 LATITUDE : 49 15.00000 N ! (deg min) LONGITUDE : 123 45.01900 W ! (deg min) WATER DEPTH : 405 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.3.1 2023/04/27 12:44:57 2 2 CLEAN 5.3 2023/04/27 12:45:14 2 2 REMOVECH 8.2 2023/04/27 12:45:19 2 2 SORT 3.6 2023/04/27 12:46:38 2 2 HDREDIT2 3.2 2023/04/27 12:46:47 ? ? $END $REMARKS -CLEAN functions: 2023/04/27 12:45:14 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag* Delete Empty Channels: 0 deleted. Set header Start and End times from the data. -REMOVECH 2023/04/27 12:45:19 The following CHANNEL(S) were removed: Date Time -SORT parameters: 2023/04/27 12:46:38 Sorted in ascending order of channel Depth:Nominal [m] -HEADER EDITS: 2023/04/27 12:46:47 Applied edit header: D:\Telework\2022-029\Processing\doc\2022-029-bot-hdr.txt Channel 1: Depth:Nominal [metres] Units: m ==> metres Format: I5 ==> F6.0 Channel 2: Sample_Number [n/a] Format: I6 ==> I5 Channel 6: Salinity:Bottle [PSS-78] Units: PSS-78PSS-78 ==> PSS-78 $END *COMMENTS Sample_Number 2: SIL: Chauvenet outlier in duplicate study Analysis methods: ----------------------- Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 4-7 days after collection. For details, see document QF2022-029SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. All samples were returned to the Institute of Ocean Sciences and they were analyzed using an Astoria-Pacific Analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document QF2022-029_NUTS*.xlsx. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in cryovials in liquid nitrogen onboard, then transferred to -80C storage in the lab until analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner Trilogy 7200-000 fluorometer calibrated wtih commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 9 weeks after the cruise. Variability is assessed as the CV% (std dev / mean*100). Chlorophyll values ranged from 0.99-15.56 ug/l in 9 samples. 0 samples were below Minimum Limit of Detection (10%, and 0 duplicate pairs had a CV>30%. Our average dataset %CV is 3.89% for 2013 - 2021 so the overall quality of this dataset is acceptable. Minimum Limit of Detection (MLD): The stated MLD for the Turner Trilogy 7200-000 fluorometer is 0.025 ug/L for CHL-a. Samples below the MLD are reported as is and assigned Quality Flag 2 with an associated comment. Where samples are taken in duplicate and the average of the two is