*2023/04/03 15:06:08.31 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2021/05/07 15:31:00.000 NUMBER OF RECORDS : 2 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 45 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 11 12 2 Pressure decibar 1.7 342.8 3 Depth metres 1.7 339.6 4 Temperature:Primary 'deg C (ITS90)' 8.7846 12.1689 5 Salinity:T0:C0 PSS-78 26.3859 31.0956 6 Fluorescence:URU:Wetlabs mg/m^3 0.69E-01 2.45 7 Oxygen:Dissolved:SBE mL/L 3.06 5.83 8 Oxygen:Dissolved:SBE umol/kg 133.6 255.2 9 Conductivity:Primary S/m 3.113871 3.335535 10 Chlorophyll:Extracted mg/m^3 2.8 2.8 11 Flag:Chlorophyll:Extracted ' ' 12 Salinity:Bottle PSS-78 26.297 31.0917 13 Flag:Salinity:Bottle ' ' 14 Nitrate_plus_Nitrite umol/L 4.81 29.36 15 Flag:Nitrate_plus_Nitrite ' ' 16 Silicate umol/L 25.05 63.05 17 Flag:Silicate ' ' 18 Phosphate umol/L 0.704 2.607 19 Flag:Phosphate ' ' 20 HPLC:Chl-c3 mg/m^3 0.17E-01 0.17E-01 21 HPLC:Chlide-a mg/m^3 0.4E-02 0.4E-02 22 HPLC:MgDVP mg/m^3 0.1 0.1 23 HPLC:Chl-c2 mg/m^3 0.131 0.131 24 HPLC:Chl-c1 mg/m^3 0 0 25 HPLC:Me-chlide mg/m^3 0.9E-02 0.9E-02 26 HPLC:Peri mg/m^3 0.8E-02 0.8E-02 27 HPLC:But-fuco mg/m^3 0 0 28 HPLC:Fuco mg/m^3 0.41E-01 0.41E-01 29 HPLC:Neo mg/m^3 0.167 0.167 30 HPLC:Pras mg/m^3 0.353 0.353 31 HPLC:Viola mg/m^3 0.85E-01 0.85E-01 32 HPLC:Hex-fuco mg/m^3 0.1E-01 0.1E-01 33 HPLC:Diadino mg/m^3 0.2E-01 0.2E-01 34 HPLC:Allo mg/m^3 0.267 0.267 35 HPLC:Diato mg/m^3 0.4E-02 0.4E-02 36 HPLC:Zea mg/m^3 0.38E-01 0.38E-01 37 HPLC:Lut mg/m^3 0.24E-01 0.24E-01 38 HPLC:Gyr-de mg/m^3 0 0 39 HPLC:Chl-b mg/m^3 1.268 1.268 40 HPLC:c2-MGDG mg/m^3 0 0 41 HPLC:DVChl-a mg/m^3 0 0 42 HPLC:Chl-a mg/m^3 3.255 3.255 43 HPLC:B-Car mg/m^3 0.71E-01 0.71E-01 44 HPLC:TChl-a mg/m^3 3.268 3.268 45 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 9 F R4 4 5 -99 ' ' 9 F R4 4 6 -99 ' ' 8 F R4 3 7 -99 ' ' 7 F R4 2 8 -99 ' ' 6 F R4 1 9 -99 ' ' 10 F R4 6 10 -99 ' ' 7 F R4 2 11 -99 ' ' 3 NQ C ' ' 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 8 F R4 3 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2021-024 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 22 EVENT NUMBER : 36 LATITUDE : 49 40.21700 N ! (deg min) LONGITUDE : 124 16.30900 W ! (deg min) WATER DEPTH : 354 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0456 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.3.1 2021/10/07 14:14:48 2 2 CLEAN 5.2.4 2021/10/07 14:19:22 2 2 REMOVECH 8.2 2021/10/07 14:19:52 2 2 SORT 3.6 2021/10/07 14:23:46 2 2 HDREDIT2 3.2 2021/10/07 14:29:18 ? ? SORT 3.6 2023/03/24 14:57:24 2 2 MERGE 3.6 2023/03/28 14:04:03 2 2 SORT 3.6 2023/03/28 14:04:15 2 2 CLEAN 5.3 2023/03/28 14:04:23 2 2 HDREDIT2 3.2 2023/03/28 14:05:16 ? ? HDREDIT2 3.2 2023/03/28 14:06:05 ? ? CLEAN 5.3 2023/04/03 15:06:08 2 2 $END $REMARKS -CLEAN functions: 2021/10/07 14:19:22 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Set event to last 4 characters of file name Set header Start and End times from the data. -REMOVECH 2021/10/07 14:19:52 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2021/10/07 14:23:46 Sorted in ascending order of channel Pressure* -HEADER EDITS: 2021/10/07 14:29:18 Applied edit header: D:\Telework\2021-024\Processing\doc\HYDRO\2021-024-bot-hdr.txt Channel 2: Pressure [decibar] Format: F9.4 ==> F7.1 -SORT parameters: 2023/03/24 14:57:24 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/28 14:04:03 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2021-024\Processing\IOS\2021-024-0036.che1 Secondary file: C:\HPLC\2021-024\Processing\IOS\2021-024-0036.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/28 14:04:15 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/28 14:04:23 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in Channel: Oxygen:Dissolved:SBE [mL/L] Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/28 14:05:16 Applied edit header: C:\HPLC\2021-024\2021-024-hdr1.txt -HEADER EDITS: 2023/03/28 14:06:05 Channel 20: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/04/03 15:06:08 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Sample_Number 11: 6 Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 46 to 51 days after the cruise. Variability is assessed as the %CV (std dev/mean*100) for duplicate samples. Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.46-5.70 ug/l in 15 samples. Average %CV for this cruise was 4.27% with 0 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30% . Our average dataset %CV is 3.83 % for 2013 - 2020 so the overall quality of this dataset is good. For details see worksheet “CV%” in file QF2021-024_CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2-3 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) fo All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2021-024HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 48 - 51 days after collection. For details, see document QF2021-024SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. All samples were returned to the Institute of Ocean Sciences and they were analyzed using an Astoria-Pacific Analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document QF2021-024_NUTS*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- Oxygen:Dissolved, Conductivity and Fluorescence:URU:Wetlabs data are nominal and unedited except that some records were removed in editing temperature and salinity. There was insufficient information from this cruise to estimate salinity accuracy but during other recent uses of this equipment it is believed to be within +/- 0.005psu. Larger errors will be present due to minor misalignment of temperature and conductivity when vertical salinity gradients are high. No recalibration was applied to the CTD salinity. Oxygen:Dissolved:SBE values cannot be confirmed as there was no calibration sampling for dissolved oxygen. Surface saturation values varied greatly. While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. It is recommended that extracted chlorophyll values be used where available. For details on the processing see document: 2021-024_Processing_Report.doc. !-1-- --2--- --3--- ---4---- ---5---- ---6--- --7--- --8-- ----9---- --10-- 11 ---12--- 13 --14-- 15 --16-- 17 ---18-- 19 ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- 45 !Samp Pressu Depth Temperat Salinity Fluores Oxygen Oxyge Conductiv Chloro Fl Salinity Fl Nitrat Fl Silica Fl Phospha Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re ure: :T0:C0 cence: : n: ity: phyll: ag :Bottle ag e_ ag te ag te ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb Primary URU: Dissol Disso Primary Extrac ~a ~o plus_ ~t ~i ~p a chlide fuco fuco : !er Wetlabs ved: lved: ted ct tt Nitrit ri ca ha HP ! SBE SBE ed le e te te te LC !---- ------ ------ -------- -------- ------- ------ ----- --------- ------ -- -------- -- ------ -- ------ -- ------- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 12 1.7 1.7 12.1689 26.3859 2.450 5.83 255.2 3.113871 2.80 0 26.2970 0 4.81 0 25.05 0 0.704 0 0.017 0.004 0.100 0.131 0.000 0.009 0.008 0.000 0.041 0.167 0.353 0.085 0.010 0.020 0.267 0.004 0.038 0.024 0.000 1.268 0.000 0.000 3.255 0.071 3.268 6 11 342.8 339.6 8.7846 31.0956 0.069 3.06 133.6 3.335535 -99.00 0 31.0917 6 29.36 6 63.05 6 2.607 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0