*2023/04/03 14:26:05.20 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2021/10/06 02:06:07.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 4 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 9AED274D NUMBER OF CHANNELS : 54 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 1 4 2 Bottle:Firing_Sequence n/a 1 4 3 Bottle_Number n/a 1 4 4 Pressure decibar 1.9 19.1 5 Depth metres 2.7 19.7 6 Temperature:Secondary 'deg C (ITS90)' 11.9191 12.446 7 Conductivity:Secondary S/m 3.388698 3.445343 8 Transmissivity %/metre 28.6 37.3 9 Transmissivity:Green %/metre 38.9 52 10 Fluorescence:URU:Seapoint mg/m^3 2.536 5.244 11 PAR uE/m^2/sec 0.3 1.6 12 Salinity:T1:C1 PSS-78 28.7603 29.7022 13 Oxygen:Dissolved:SBE mL/L 4.33 7.34 14 Oxygen:Dissolved:SBE umol/kg 189 320.9 15 Number_of_bin_records n/a 241 241 16 Temperature:Draw 'deg C (ITS90)' 11.6 12.3 17 Chlorophyll:Extracted mg/m^3 6.2 25.84 18 Flag:Chlorophyll:Extracted ' ' 19 Phaeo-Pigment:Extracted mg/m^3 -0.26 0.27 20 Oxygen:Dissolved mL/L 3.476 7.213 21 Oxygen:Dissolved umol/kg 153.9 319.3 22 Flag:Oxygen:Dissolved ' ' 23 Nitrate_plus_Nitrite umol/L 1.75 18.42 24 Flag:Nitrate_plus_Nitrite ' ' 25 Silicate umol/L 19.52 40.42 26 Flag:Silicate ' ' 27 Phosphate umol/L 0.549 1.878 28 Flag:Phosphate ' ' 29 HPLC:Chl-c3 mg/m^3 0.75E-01 0.75E-01 30 HPLC:Chlide-a mg/m^3 0.254 0.254 31 HPLC:MgDVP mg/m^3 0.62E-01 0.62E-01 32 HPLC:Chl-c2 mg/m^3 0.781 0.781 33 HPLC:Chl-c1 mg/m^3 0.446 0.446 34 HPLC:Me-chlide mg/m^3 0.119 0.119 35 HPLC:Peri mg/m^3 0.63E-01 0.63E-01 36 HPLC:But-fuco mg/m^3 0.12E-01 0.12E-01 37 HPLC:Fuco mg/m^3 3.279 3.279 38 HPLC:Neo mg/m^3 0 0 39 HPLC:Pras mg/m^3 0.17E-01 0.17E-01 40 HPLC:Viola mg/m^3 0.9E-02 0.9E-02 41 HPLC:Hex-fuco mg/m^3 0.39E-01 0.39E-01 42 HPLC:Diadino mg/m^3 0.269 0.269 43 HPLC:Allo mg/m^3 0.2E-01 0.2E-01 44 HPLC:Diato mg/m^3 0.12E-01 0.12E-01 45 HPLC:Zea mg/m^3 0.5E-02 0.5E-02 46 HPLC:Lut mg/m^3 0 0 47 HPLC:Gyr-de mg/m^3 0.4E-02 0.4E-02 48 HPLC:Chl-b mg/m^3 0.35E-01 0.35E-01 49 HPLC:c2-MGDG mg/m^3 0.17E-01 0.17E-01 50 HPLC:DVChl-a mg/m^3 0 0 51 HPLC:Chl-a mg/m^3 5.731 5.731 52 HPLC:B-Car mg/m^3 0.96E-01 0.96E-01 53 HPLC:TChl-a mg/m^3 6.105 6.105 54 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 10 F ' ' 6 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 6 F ' ' 1 10 -99 ' ' 8 F ' ' 3 11 -99 ' ' 7 F ' ' 1 12 -99 ' ' 9 F ' ' 4 13 -99 ' ' 7 F ' ' 2 14 -99 ' ' 6 F ' ' 1 15 -99 ' ' 5 I I 0 16 -99 ' ' 6 F R4 1 17 -99 ' ' 7 F R4 2 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 8 F R4 3 21 -99 ' ' 6 F ' ' 1 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 8 F R4 3 28 -99 ' ' 3 NQ C ' ' 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2021-022 AGENCY : IOS, Ocean Science Division, Sidney, B.C. COUNTRY : Canada PROJECT : Salish Sea Biophysical Survey SCIENTIST : Chandler P. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : Strait of Georgia / Juan de Fuca Strait STATION : SI EVENT NUMBER : 1 LATITUDE : 48 39.02000 N ! (deg min) LONGITUDE : 123 30.06000 W ! (deg min) WATER DEPTH : 187 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 4700 Conductivity ' ' 3531 'Pressure:Digiquartz with TC' ' ' 0443 Temperature:2 ' ' 4888 Conductivity:2 ' ' 4513 'Oxygen:SBE 43' ' ' 3791 Fluorometer:Seapoint ' ' 3950 Altimeter ' ' 76341 'Transmissometer:WET Labs C-Star' ' ' 1185DR 'Transmissometer:WET Labs C-Star:2' ' ' 1883DG pH ' ' 0691 PAR/Irradiance:Biospherical/Licor ' ' 4565 Unavailable ' ' ' ' 'SPAR/Surface Irradiance' ' ' 20518 $END $REMARKS Software Version Seasave V 7.26.7.121 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.3 2022/02/04 13:48:52 964 964 CLEAN 5.3 2022/02/04 13:51:02 964 964 ADDSAMP 3.6 2022/02/04 14:26:39 964 964 BINAVE 4.2.1 2022/02/04 14:27:04 964 4 MERGE 3.6 2022/02/08 12:49:10 4 4 CLEAN 5.3 2022/02/11 11:11:23 4 4 CALIB 12.0.1 2022/02/11 11:11:33 4 4 SORT 3.6 2022/02/11 15:08:54 4 4 REMOVECH 8.2 2022/02/11 15:10:45 4 4 CHGUNITS 3.2 2022/02/11 15:13:14 4 4 CHGUNITS 3.2 2022/02/11 15:13:44 4 4 REORDER 1.3.1 2022/02/11 16:13:50 ? ? HDREDIT2 3.2 2022/02/11 16:13:55 ? ? SORT 3.6 2023/03/23 14:46:32 4 4 MERGE 3.6 2023/03/28 13:57:12 4 4 SORT 3.6 2023/03/28 13:57:23 4 4 CLEAN 5.3 2023/03/28 13:57:32 4 4 HDREDIT2 3.2 2023/03/28 14:00:35 ? ? HDREDIT2 3.2 2023/03/28 14:01:46 ? ? CLEAN 5.3 2023/04/03 14:26:05 4 4 $END $REMARKS -CLEAN functions: 2022/02/04 13:51:02 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: D:\Telework\2021-022\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2022/02/08 12:49:10 Merge Channel: Bottle_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate Primary file : D:\Telework\2021-022\Processing\hydro\2021-022-0001.samavg Secondary file: D:\Telework\2021-022\Processing\hydro\2021-022-0001.mrgcln1s 4 secondary records matched to primary records. -SORT parameters: 2022/02/07 14:11:07 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2022/02/11 11:11:23 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Set event to last 4 characters of file name Remove remark heading: "Comments from secondary" -CALIB parameters: 2022/02/11 11:11:32 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = D:\Telework\2021-022\Processing\doc\2021-022-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 4 Pressure decibar 10 -0.8000000E+00 0.1000000E+01 22 Oxygen:Dissolved:SBE mL/L 10 0.1200000E-02 0.1046500E+01 -SORT parameters: 2022/02/11 15:08:54 Sorted in ascending order of channel Pressure -REMOVECH 2022/02/11 15:10:45 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Oxygen:Voltage:SBE [volts] PAR:Reference [uE/m^2/sec] pH:SBE [pH Units] Status:Pump Altimeter [metres] Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Secondary Salinity reference channel: Salinity:T1:C1 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Secondary Salinity reference channel: Temperature:Draw 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2022/02/11 16:13:55 Applied edit header: D:\Telework\2021-022\Processing\doc\HYDRO\2021-022-bot-hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F9.4 ==> F7.1 Channel 7: Transmissivity [%/metre] Name: Transmissivity:1 ==> Transmissivity Format: F9.4 ==> F6.1 Channel 8: Transmissivity:Green [%/metre] Name: Transmissivity:2 ==> Transmissivity:Green Channel 16: Temperature:Draw [deg C (ITS90)] Format: F7.2 ==> F6.1 -SORT parameters: 2023/03/23 14:46:32 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/28 13:57:12 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2021-022\Processing\IOS\2021-022-0001.che1 Secondary file: C:\HPLC\2021-022\Processing\IOS\2021-022-0001.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/28 13:57:23 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/28 13:57:32 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in Channel: Oxygen:Dissolved:SBE [mL/L] Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/28 14:00:35 Applied edit header: C:\HPLC\2021-022\2021-022-hdr1.txt -HEADER EDITS: 2023/03/28 14:01:46 Applied edit header: C:\HPLC\2021-022\2021-022-hdr2.txt Channel 29: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/04/03 14:26:05 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS SBE HEADER Sea-Bird SBE 9 Data File: FileName = C:\CTD data\2021-022\2021-022-0001.hdr Software Version Seasave V 7.26.7.121 Temperature SN = 4700 Conductivity SN = 3531 Number of Bytes Per Scan = 40 Number of Voltage Words = 5 Number of Scans Averaged by the Deck Unit = 1 System UpLoad Time = Oct 06 2021 02:06:07 NMEA Latitude = 48 39.02 N NMEA Longitude = 123 30.06 W NMEA UTC (Time) = Oct 06 2021 02:02:39 Store Lat/Lon Data = Append to Every Scan SBE 11plus V 5.1e number of scans to average = 1 pressure baud rate = 9600 NMEA baud rate = 4800 surface PAR voltage added to scan A/D offset = 0 GPIB address = 1 advance primary conductivity 0.073 seconds advance secondary conductivity 0.073 seconds S> Tully: 2021-022 Station: SI Depth: 187 System UTC = Oct 06 2021 02:06:07 # nquan = 23 # nvalues = 964 # units = specified # name 0 = scan: Scan Count # name 1 = bpos: Bottle Position in Carousel # name 2 = nbf: Bottles Fired # name 3 = prDM: Pressure, Digiquartz [db] # name 4 = depSM: Depth [salt water, m] # name 5 = t090C: Temperature [ITS-90, deg C] # name 6 = t190C: Temperature, 2 [ITS-90, deg C] # name 7 = c0S/m: Conductivity [S/m] # name 8 = c1S/m: Conductivity, 2 [S/m] # name 9 = CStarTr0: Beam Transmission, WET Labs C-Star [%] # name 10 = CStarTr1: Beam Transmission, WET Labs C-Star, 2 [%] # name 11 = flSP: Fluorescence, Seapoint # name 12 = sbeox0V: Oxygen raw, SBE 43 [V] # name 13 = par: PAR/Irradiance, Biospherical/Licor # name 14 = spar: SPAR/Surface Irradiance # name 15 = ph: pH # name 16 = pumps: Pump Status # name 17 = altM: Altimeter [m] # name 18 = dz/dtM: Descent Rate [m/s] # name 19 = sal00: Salinity, Practical [PSU] # name 20 = sal11: Salinity, Practical, 2 [PSU] # name 21 = sbeox0ML/L: Oxygen, SBE 43 [ml/l] # name 22 = flag: 0.000e+00 # interval = seconds: 0.0416667 # start_time = Oct 06 2021 02:06:07 [System UTC, header] # bad_flag = -9.990e-29 # # # # # 4700 # 12-Dec-20 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.34744496e-003 # 6.38231870e-004 # 2.10953096e-005 # 1.79186371e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 3531 # 06-Jan-21 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.00921127e+001 # 1.54474443e+000 # -2.54963711e-003 # 2.95252222e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 0443 # 07-Jan-21 # -4.435292e+004 # -1.635794e-001 # 1.192740e-002 # 3.979900e-002 # 0.000000e+000 # 2.993627e+001 # -3.271008e-004 # 3.759160e-006 # 1.565300e-009 # 0.99989502 # -0.38836 # 0.000000e+000 # 1.282462e-002 # -8.599824e+000 # # # # # # 4888 # 12-Dec-20 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.32604412e-003 # 6.34365670e-004 # 2.18217362e-005 # 2.02364598e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 4513 # 18-Dec-20 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.00676289e+001 # 1.38093586e+000 # -1.32984366e-003 # 1.69158499e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 3791 # 22-Dec-20 # 1 # # # 0.0000 # 0.0000e+000 # 0.0000 # 0.00e+000 # 0.0000 # 0.0 # # # # 4.1609e-001 # -0.7169 # -4.3461e-003 # 1.7263e-004 # -3.0839e-006 # 2.5826e+000 # 1.92634e-004 # -4.64803e-002 # 3.6000e-002 # 1.4600 #

-3.3000e-002

#

5.0000e+003

#

1.4500e+003

# # # # # # # 3950 # # # 2 # 0.000 # # # # # # 76341 # 2021-02-10 # 15.000 # 0.000 # # # # # # # # # 1185DR # 2021-04-28 # 19.7050 # -1.0828 # 0.250 # # # # # # 1883DG # 2021-04-28 # 20.5546 # -0.0502 # 0.250 # # # # # # 0691 # 23-Feb-21 # 4.6068 # 2.5090 # # # # # # 4565 # 2021-02-24 # 1.00000000 # 0.00000000 # 3802281368.82000020 # 1.00000000 # -0.03962000 # # # # # # # # # 20518 # 20210224 # 1.00000000 # 1504.23400000 # # # # datcnv_date = Feb 04 2022 13:46:25, 7.23.2 # datcnv_in = D:\Telework\2021-022\Unprocessed\ctd\2021-022-0001.hex D:\Telework \2021-022\Processing\doc\2021-022-ctd.xmlcon # datcnv_ox_hysteresis_correction = no # datcnv_ox_tau_correction = yes # datcnv_bottle_scan_range_source = BL file # datcnv_scans_per_bottle = 241 END* Sample_Number 2: ALL: Niskin vent was loose causing a leak. Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 7-8 weeks after the cruise. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Chlorophyll values ranged from 0.17-25.84ug/l in 112 samples. Average %CV for this cruise was 3.43% with 1 out of 28 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.83% for 2013 - 2020 so the overall quality of this dataset is good. For details see worksheet “CV%” in file QF2021-022_CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Peña, 2014. Analysis was performed ~8-10 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for du All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2021-022HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2021-022OXY*.xlsx. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 7 weeks of collection. For details including a duplicate analysis, see document QF2021-022SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. For samples 400m and deeper a second set of samples are collected and stored cool for silicate analysis. All samples were returned to the Insitute of Ocean Sciences and they were analyzed using an Astoria-Pacific Analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2021-022nuts*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminot, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Conductivity, Transmissivity, Fluorescence:URU:Seapoint, PAR and pH:SBE data are nominal and unedited except that some records were removed in editing temperature and salinity. The Reference PAR sensor produced no useful data. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. Channel Salinity:T1:C1 was found to be lower than bottles by approximately 0.003psu, but delayed analysis and incomplete flushing of Niskin bottles is likely to result in bottle values being higher than ambient salinity values by roughly 0.005psu, so the CTD salinity was likely high by roughly 0.002psu. During use in the previous cruise the secondary salinity was considered to be low by about 0.0005psu. No recalibration was applied due to the uncertainty in the comparisons. Salinity is likely within 0.002psu. For details on the processing see the report: 2021-022_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: C:\HPLC\2021-022\Processing\IOS\2021-022-0001.hplc -------------------------------------------------- Sample_Number 4: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Pressure decibar 10 -99 () (-0.8 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.12E-02 1.0465) $END !-1-- 2- 3- --4--- --5--- ---6---- ----7---- --8-- --9-- ---10-- --11-- ---12--- --13-- --14- -15- --16- --17-- 18 --19-- ---20-- --21- 22 --23-- 24 --25-- 26 ---27-- 28 ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- 54 !Samp Bo Bo Pressu Depth Temperat Conductiv Trans Trans Fluores PAR Salinity Oxygen Oxyge Numb Tempe Chloro Fl Phaeo- Oxygen: Oxyge Fl Nitrat Fl Silica Fl Phospha Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: ity: missi missi cence: :T1:C1 : n: er_o ratur phyll: ag Pigmen Dissolv n: ag e_ ag te ag te ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Secondar Secondary vity vity: URU: Dissol Disso ~bin e: Extrac ~a t: ed Disso ~o plus_ ~t ~i ~p a chlide fuco fuco : !er en mb y Green Seapoin ved: lved: _rec Draw ted ct Extrac lved lv Nitrit ri ca ha HP ! ce er t SBE SBE ords ed ted ed e te te te LC !---- -- -- ------ ------ -------- --------- ----- ----- ------- ------ -------- ------ ----- ---- ----- ------ -- ------ ------- ----- -- ------ -- ------ -- ------- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 4 4 4 1.9 2.7 12.4460 3.388698 37.3 52.0 2.536 1.6 28.7603 7.34 320.9 241 12.3 6.20 0 0.08 7.213 319.3 0 1.75 0 19.52 0 0.549 0 0.075 0.254 0.062 0.781 0.446 0.119 0.063 0.012 3.279 0.000 0.017 0.009 0.039 0.269 0.020 0.012 0.005 0.000 0.004 0.035 0.017 0.000 5.731 0.096 6.105 36 3 3 3 4.3 5.0 12.1905 3.407923 28.6 39.9 5.164 0.9 29.1380 6.47 282.9 241 12.1 17.57 0 0.08 6.775 299.9 0 3.49 0 24.99 0 0.931 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 2 2 2 9.3 10.0 11.9635 3.414923 29.0 38.9 5.244 0.4 29.3803 5.53 241.7 241 11.8 25.84 3 -0.26 5.462 241.8 36 10.86 3 33.04 3 1.557 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1 1 1 19.1 19.7 11.9191 3.445343 30.2 41.6 3.745 0.3 29.7022 4.33 189.0 241 11.6 10.30 6 0.27 3.476 153.9 0 18.42 0 40.42 0 1.878 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0