*2023/04/03 12:06:03.36 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2021/04/10 22:25:51.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 4 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 7C18A883 NUMBER OF CHANNELS : 49 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 251 254 2 Bottle:Firing_Sequence n/a 1 4 3 Bottle_Number n/a 1 4 4 Pressure decibar 2.7 20.1 5 Depth metres 2.7 19.9 6 Temperature:Primary 'deg C (ITS90)' 8.536 8.7813 7 Conductivity:Primary S/m 3.06912 3.074635 8 Transmissivity %/metre 27.3 39.4 9 Fluorescence:URU:Seapoint mg/m^3 2.146 6.438 10 PAR uE/m^2/sec 1 543.1 11 Salinity:T0:C0 PSS-78 28.5155 28.7431 12 Oxygen:Dissolved:SBE mL/L 6.96 7.86 13 Oxygen:Dissolved:SBE umol/kg 304 343.3 14 Number_of_bin_records n/a 241 241 15 Chlorophyll:Extracted mg/m^3 5.54 11.12 16 Flag:Chlorophyll:Extracted ' ' 17 Phaeo-Pigment:Extracted mg/m^3 2.43 3.25 18 Nitrate_plus_Nitrite umol/L 5.27 12.8 19 Flag:Nitrate_plus_Nitrite ' ' 20 Silicate umol/L 13.08 23.22 21 Flag:Silicate ' ' 22 Phosphate umol/L 0.876 1.464 23 Flag:Phosphate ' ' 24 HPLC:Chl-c3 mg/m^3 0.239 0.26 25 HPLC:Chlide-a mg/m^3 0.54 0.827 26 HPLC:MgDVP mg/m^3 0.6E-01 0.67E-01 27 HPLC:Chl-c2 mg/m^3 1.524 1.572 28 HPLC:Chl-c1 mg/m^3 0.411 0.429 29 HPLC:Me-chlide mg/m^3 0.89 0.949 30 HPLC:Peri mg/m^3 0.85E-01 0.94E-01 31 HPLC:But-fuco mg/m^3 0.17E-01 0.17E-01 32 HPLC:Fuco mg/m^3 4.521 4.712 33 HPLC:Neo mg/m^3 0 0.6E-02 34 HPLC:Pras mg/m^3 0 0 35 HPLC:Viola mg/m^3 0.1E-01 0.17E-01 36 HPLC:Hex-fuco mg/m^3 0 0 37 HPLC:Diadino mg/m^3 0.502 0.605 38 HPLC:Allo mg/m^3 0.25E-01 0.38E-01 39 HPLC:Diato mg/m^3 0.93E-01 0.12 40 HPLC:Zea mg/m^3 0.21E-01 0.25E-01 41 HPLC:Lut mg/m^3 0 0 42 HPLC:Gyr-de mg/m^3 0 0.6E-02 43 HPLC:Chl-b mg/m^3 0 0 44 HPLC:c2-MGDG mg/m^3 0 0 45 HPLC:DVChl-a mg/m^3 0 0 46 HPLC:Chl-a mg/m^3 5.473 6.261 47 HPLC:B-Car mg/m^3 0.106 0.114 48 HPLC:TChl-a mg/m^3 7.249 7.691 49 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 10 F ' ' 6 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 8 F ' ' 3 10 -99 ' ' 7 F ' ' 1 11 -99 ' ' 9 F ' ' 4 12 -99 ' ' 7 F ' ' 2 13 -99 ' ' 6 F ' ' 1 14 -99 ' ' 5 I I 0 15 -99 ' ' 7 F R4 2 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 7 F R4 2 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 8 F R4 3 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2021-020 AGENCY : IOS, Ocean Science Division, Sidney, B.C. COUNTRY : Canada PROJECT : Salish Sea Spring Biophysical Survey SCIENTIST : Chandler P. PLATFORM : Vector *LOCATION GEOGRAPHIC AREA : Strait of Georgia / Juan de Fuca Strait STATION : 22 EVENT NUMBER : 76 LATITUDE : 49 40.25000 N ! (deg min) LONGITUDE : 124 16.31000 W ! (deg min) WATER DEPTH : 356 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 4700 Conductivity ' ' 3531 'Pressure:Digiquartz with TC' ' ' 0443 Temperature:2 ' ' 4888 Conductivity:2 ' ' 4513 'Oxygen:SBE 43' ' ' 3791 Fluorometer:Seapoint ' ' 3950 Altimeter ' ' 67341 pH ' ' 0692 PAR/Irradiance:Biospherical/Licor ' ' '4565 QSP200L4S' 'Transmissometer:WET Labs C-Star' ' ' 983DR Unavailable ' ' ' ' 'SPAR/Surface Irradiance' ' ' 16504 $END $REMARKS Software Version Seasave V 7.26.7.121 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.3 2021/08/03 09:22:11 964 964 CLEAN 5.2.4 2021/08/03 09:45:26 964 964 ADDSAMP 3.6 2021/08/03 09:54:28 964 964 BINAVE 4.2.1 2021/08/03 09:56:18 964 4 MERGE 3.6 2021/08/04 09:40:44 4 4 CLEAN 5.2.4 2021/08/09 11:19:36 4 4 CALIB 12.0.1 2021/08/24 14:17:27 4 4 SORT 3.6 2021/08/25 10:48:12 4 4 REMOVECH 8.2 2021/08/25 10:50:09 4 4 CHGUNITS 3.2 2021/08/25 10:52:53 4 4 CHGUNITS 3.2 2021/08/25 10:52:57 4 4 REORDER 1.3.1 2021/08/25 10:53:33 ? ? HDREDIT2 3.2 2021/08/25 13:52:49 ? ? SORT 3.6 2023/03/23 14:27:13 4 4 MERGE 3.6 2023/03/28 12:52:53 4 4 SORT 3.6 2023/03/28 12:53:09 4 4 CLEAN 5.3 2023/03/28 12:53:20 4 4 HDREDIT2 3.2 2023/03/28 13:01:15 ? ? HDREDIT2 3.2 2023/03/28 13:01:50 ? ? CLEAN 5.3 2023/04/03 12:06:03 4 4 $END $REMARKS -CLEAN functions: 2021/08/03 09:45:26 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: D:\Telework\2021-020\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2021/08/04 09:40:44 Merge Channel: Bottle_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide, Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolved, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total Primary file : D:\Telework\2021-020\Processing\hydro\2021-020-0076.samavg Secondary file: D:\Telework\2021-020\Processing\hydro\2021-020-0076.mrgcln1s 4 secondary records matched to primary records. -SORT parameters: 2021/08/04 09:40:32 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2021/08/09 11:19:34 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Remove remark heading: "Comments from secondary" -CALIB parameters: 2021/08/24 14:17:27 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = D:\Telework\2021-020\Processing\doc\2021-020-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 21 Oxygen:Dissolved:SBE mL/L 10 -0.1310000E-01 0.1051500E+01 -SORT parameters: 2021/08/25 10:48:12 Sorted in ascending order of channel Pressure -REMOVECH 2021/08/25 10:50:09 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] pH:SBE [pH Units] PAR:Reference [uE/m^2/sec] Status:Pump Altimeter [metres] Descent_Rate [m/s] Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary [deg C (ITS90)] Salinity reference channel: Salinity:T0:C0 [PSS-78] 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 [PSS-78] - No units changed -HEADER EDITS: 2021/08/25 13:52:49 Applied edit header: D:\Telework\2021-020\Processing\doc\HYDRO\2021-020-bot-hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F9.4 ==> F7.1 Channel 7: Transmissivity [%/metre] Name: Transmissivity:1 ==> Transmissivity Format: F9.4 ==> F6.1 -SORT parameters: 2023/03/23 14:27:13 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/28 12:52:53 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2021-020\Processing\IOS\2021-020-0076.che1 Secondary file: C:\HPLC\2021-020\Processing\IOS\2021-020-0076.hplc 2 secondary records matched to primary records. -SORT parameters: 2023/03/28 12:53:09 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/28 12:53:20 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in Channel: Oxygen:Dissolved:SBE [mL/L] Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/28 13:01:15 Applied edit header: C:\HPLC\2021-020\2021-020-hdr1.txt -HEADER EDITS: 2023/03/28 13:01:50 Applied edit header: C:\HPLC\2021-020\2021-020-hdr2.txt Channel 24: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/04/03 12:06:01 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS SBE HEADER Sea-Bird SBE 9 Data File: FileName = C:\ctddata\2021-020\2021-020-0076.hdr Software Version Seasave V 7.26.7.121 Temperature SN = 4700 Conductivity SN = 3531 Number of Bytes Per Scan = 40 Number of Voltage Words = 5 Number of Scans Averaged by the Deck Unit = 1 System UpLoad Time = Apr 10 2021 15:25:51 NMEA Latitude = 49 40.25 N NMEA Longitude = 124 16.31 W NMEA UTC (Time) = Apr 10 2021 22:24:12 Store Lat/Lon Data = Append to Every Scan SBE 11plus V 5.1e number of scans to average = 1 pressure baud rate = 9600 NMEA baud rate = 4800 surface PAR voltage added to scan A/D offset = 5 GPIB address = 1 advance primary conductivity 0.073 seconds advance secondary conductivity 0.073 seconds advance voltage 6 0.000 seconds advance voltage 7 0.000 seconds S> Ship Vector: Station: 22 Depth: 356 System UTC = Apr 10 2021 22:25:51 # nquan = 22 # nvalues = 964 # units = specified # name 0 = scan: Scan Count # name 1 = bpos: Bottle Position in Carousel # name 2 = nbf: Bottles Fired # name 3 = prDM: Pressure, Digiquartz [db] # name 4 = depSM: Depth [salt water, m] # name 5 = t090C: Temperature [ITS-90, deg C] # name 6 = t190C: Temperature, 2 [ITS-90, deg C] # name 7 = c0S/m: Conductivity [S/m] # name 8 = c1S/m: Conductivity, 2 [S/m] # name 9 = CStarTr0: Beam Transmission, WET Labs C-Star [%] # name 10 = flSP: Fluorescence, Seapoint # name 11 = sbeox0V: Oxygen raw, SBE 43 [V] # name 12 = ph: pH # name 13 = par: PAR/Irradiance, Biospherical/Licor # name 14 = spar: SPAR/Surface Irradiance # name 15 = pumps: Pump Status # name 16 = altM: Altimeter [m] # name 17 = dz/dtM: Descent Rate [m/s] # name 18 = sal00: Salinity, Practical [PSU] # name 19 = sal11: Salinity, Practical, 2 [PSU] # name 20 = sbeox0ML/L: Oxygen, SBE 43 [ml/l] # name 21 = flag: 0.000e+00 # interval = seconds: 0.0416667 # start_time = Apr 10 2021 22:25:51 [System UTC, header] # bad_flag = -9.990e-29 # # # # # 4700 # 12-Dec-20 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.34744496e-003 # 6.38231870e-004 # 2.10953096e-005 # 1.79186371e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 3531 # 06-Jan-21 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.00921127e+001 # 1.54474443e+000 # -2.54963711e-003 # 2.95252222e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 0443 # 07-Jan-21 # -4.435292e+004 # -1.635794e-001 # 1.192740e-002 # 3.979900e-002 # 0.000000e+000 # 2.993627e+001 # -3.271008e-004 # 3.759160e-006 # 1.565300e-009 # 0.99989502 # -0.38836 # 0.000000e+000 # 1.282462e-002 # -8.599824e+000 # # # # # # 4888 # 12-Dec-20 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.32604412e-003 # 6.34365670e-004 # 2.18217362e-005 # 2.02364598e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 4513 # 18-Dec-20 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.00676289e+001 # 1.38093586e+000 # -1.32984366e-003 # 1.69158499e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 3791 # 22-Dec-20 # 1 # # # 0.0000 # 0.0000e+000 # 0.0000 # 0.00e+000 # 0.0000 # 0.0 # # # # 4.1609e-001 # -0.7169 # -4.3461e-003 # 1.7263e-004 # -3.0839e-006 # 2.5826e+000 # 1.92634e-004 # -4.64803e-002 # 3.6000e-002 # 1.4600 #

-3.3000e-002

#

5.0000e+003

#

1.4500e+003

# # # # # # # 3950 # # # 1 # 0.000 # # # # # # 67341 # # 15.000 # 1.000 # # # # # # # # # 0692 # 14-Jan-20 # 4.6304 # 2.5153 # # # # # # 4565 QSP200L4S # 16-Mar-2011 # 1.00000000 # 0.00000000 # 4115226337.44999980 # 1.00000000 # -0.36780000 # # # # # # 983DR # Oct 2020 # 19.6589 # 0.5308 # 0.250 # # # # # # # # # # # # 16504 # 03-March-2016 # 1508.20000000 # 1.00000000 # # # # datcnv_date = Aug 03 2021 08:54:24, 7.23.2 # datcnv_in = D:\Telework\2021-020\Unprocessed\CTD\2021-020-0076.hex D:\Telework \2021-020\Processing\doc\2021-020-ctd1.XMLCON # datcnv_ox_hysteresis_correction = no # datcnv_ox_tau_correction = yes # datcnv_bottle_scan_range_source = BL file # datcnv_scans_per_bottle = 241 END* Sample_Number 251: CHL: bulb loosing power, correction factor applied Sample_Number 252: CHL: bulb loosing power, correction factor applied Sample_Number 253: CHL: bulb loosing power, correction factor applied Sample_Number 254: CHL: bulb loosing power, correction factor applied Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 1-7 weeks after the cruise. Variability is assessed as the %CV (std dev/mean*100) for duplicate samples. Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.01-36.24 ug/l in 202 samples. Average %CV for this cruise was 4.89% with 3 out of 51 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30% . Our average dataset %CV is 3.83 % for 2013 - 2020 so the overall quality of this dataset is good. For details see worksheet “CV%” in file QF2021-020_CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Peña, 2014. Analysis was performed ~1 month after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) fo All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2021-020HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2021-020OXY*.xlsx. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 108 - 116 days after collection. For details including a duplicate analysis, see document QF2021-020SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. For samples 400m and deeper a second set of samples are collected and stored cool for silicate analysis. All samples were returned to the Insitute of Ocean Sciences and they were analyzed using an Astoria-Pacific Analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2021-020nuts*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal. No useful data were obtained from the Surface PAR and pH channels, so those channels were removed. The transmissometer wavelength for the sensor used during this cruise is 650nm. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. Dissolved oxygen was calibrated using the method described in SeaBird Application Note #64-2, June 2012 revision, except that a small offset in the fit was allowed. Oxygen:Dissolved:SBE data were recalibrated based on the open-ocean section of cruise 2021-006 when the same equipment was used. For details on the processing see the report: 2021-020_Processing_Report.doc. ----------------------------------------------------------------------------------- Comments from secondary file: C:\HPLC\2021-020\Processing\IOS\2021-020-0076.hplc -------------------------------------------------- Sample_Number 254: HPLC: filters still a bit green, values may be underestimate *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (-0.131E-01 1.0515) $END !-1-- 2- 3- --4--- --5--- ---6---- ----7---- --8-- ---9--- --10-- ---11--- --12-- --13- -14- --15-- 16 --17-- --18-- 19 --20-- 21 ---22-- 23 ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- 49 !Samp Bo Bo Pressu Depth Temperat Conductiv Trans Fluores PAR Salinity Oxygen Oxyge Numb Chloro Fl Phaeo- Nitrat Fl Silica Fl Phospha Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: ity: missi cence: :T0:C0 : n: er_o phyll: ag Pigmen e_ ag te ag te ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Primary Primary vity URU: Dissol Disso ~bin Extrac ~a t: plus_ ~t ~i ~p a chlide fuco fuco : !er en mb Seapoin ved: lved: _rec ted ct Extrac Nitrit ri ca ha HP ! ce er t SBE SBE ords ed ted e te te te LC !---- -- -- ------ ------ -------- --------- ----- ------- ------ -------- ------ ----- ---- ------ -- ------ ------ -- ------ -- ------- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 254 4 4 2.7 2.7 8.7813 3.071197 27.3 2.146 543.1 28.5164 7.85 343.2 241 10.37 2 2.49 5.27 0 13.08 0 0.925 0 0.239 0.827 0.067 1.572 0.429 0.949 0.085 0.017 4.521 0.000 0.000 0.017 0.000 0.605 0.038 0.120 0.025 0.000 0.006 0.000 0.000 0.000 5.473 0.106 7.249 26 253 3 3 5.0 5.0 8.7778 3.070930 27.4 4.542 189.5 28.5155 7.86 343.3 241 11.12 2 2.43 5.43 0 13.47 0 0.876 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 252 2 2 10.1 10.0 8.7110 3.069120 27.6 6.438 21.8 28.5486 7.72 337.3 241 10.32 2 3.25 6.98 0 14.49 0 1.032 0 0.260 0.540 0.060 1.524 0.411 0.890 0.094 0.017 4.712 0.006 0.000 0.010 0.000 0.502 0.025 0.093 0.021 0.000 0.000 0.000 0.000 0.000 6.261 0.114 7.691 6 251 1 1 20.1 19.9 8.5360 3.074635 39.4 3.546 1.0 28.7431 6.96 304.0 241 5.54 26 2.92 12.80 6 23.22 6 1.464 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0