*2023/03/31 16:28:46.65 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2020/02/18 15:31:00.000 NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 46 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 3 3 2 Pressure decibar 1.1 1.1 3 Depth metres 1.1 1.1 4 Temperature:Primary 'deg C (ITS90)' 6.5627 6.5627 5 Salinity:T0:C0 PSS-78 25.8803 25.8803 6 Fluorescence:URU:Wetlabs mg/m^3 2.435 2.435 7 Oxygen:Dissolved:SBE mL/L 7.3 7.3 8 Oxygen:Dissolved:SBE umol/kg 319.6 319.6 9 Conductivity:Primary S/m 2.654517 2.654517 10 Salinity:Bottle PSS-78 -99 -99 11 Flag:Salinity:Bottle ' ' 12 Nitrate_plus_Nitrite umol/L 25.98 25.98 13 Flag:Nitrate_plus_Nitrite ' ' 14 Silicate umol/L 59.33 59.33 15 Flag:Silicate ' ' 16 Phosphate umol/L 1.978 1.978 17 Flag:Phosphate ' ' 18 Chlorophyll:Extracted mg/m^3 1.11 1.11 19 Flag:Chlorophyll:Extracted ' ' 20 Phaeo-Pigment:Extracted mg/m^3 0.43 0.43 21 HPLC:Chl-c3 mg/m^3 0.19E-01 0.19E-01 22 HPLC:Chlide-a mg/m^3 0.2E-02 0.2E-02 23 HPLC:MgDVP mg/m^3 0.2E-01 0.2E-01 24 HPLC:Chl-c2 mg/m^3 0.76E-01 0.76E-01 25 HPLC:Chl-c1 mg/m^3 0.6E-02 0.6E-02 26 HPLC:Me-chlide mg/m^3 0.4E-02 0.4E-02 27 HPLC:Peri mg/m^3 0.9E-02 0.9E-02 28 HPLC:But-fuco mg/m^3 0.5E-02 0.5E-02 29 HPLC:Fuco mg/m^3 0.178 0.178 30 HPLC:Neo mg/m^3 0.27E-01 0.27E-01 31 HPLC:Pras mg/m^3 0.42E-01 0.42E-01 32 HPLC:Viola mg/m^3 0.3E-01 0.3E-01 33 HPLC:Hex-fuco mg/m^3 0.3E-02 0.3E-02 34 HPLC:Diadino mg/m^3 0.22E-01 0.22E-01 35 HPLC:Allo mg/m^3 0.84E-01 0.84E-01 36 HPLC:Diato mg/m^3 0 0 37 HPLC:Zea mg/m^3 0.8E-02 0.8E-02 38 HPLC:Lut mg/m^3 0.17E-01 0.17E-01 39 HPLC:Gyr-de mg/m^3 0 0 40 HPLC:Chl-b mg/m^3 0.182 0.182 41 HPLC:c2-MGDG mg/m^3 0.2E-02 0.2E-02 42 HPLC:DVChl-a mg/m^3 0 0 43 HPLC:Chl-a mg/m^3 0.96 0.96 44 HPLC:B-Car mg/m^3 0.17E-01 0.17E-01 45 HPLC:TChl-a mg/m^3 0.966 0.966 46 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 9 F R4 4 5 -99 ' ' 9 F R4 4 6 -99 ' ' 8 F R4 3 7 -99 ' ' 7 F R4 2 8 -99 ' ' 6 F R4 1 9 -99 ' ' 10 F R4 6 10 -99 ' ' 9 F R4 4 11 -99 ' ' 3 NQ C ' ' 12 -99 ' ' 7 F R4 2 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2020-047 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 3 EVENT NUMBER : 7 LATITUDE : 49 26.63400 N ! (deg min) LONGITUDE : 124 20.20800 W ! (deg min) WATER DEPTH : 323 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0573 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.3.1 2020/07/30 15:36:40 1 1 CLEAN 5.2.4 2020/07/30 15:37:28 1 1 REMOVECH 8.2 2020/07/30 15:38:38 1 1 SORT 3.6 2020/07/30 16:43:31 1 1 HDREDIT2 3.2 2020/07/30 16:50:43 ? ? CLEAN 5.2.4 2021/04/14 13:41:43 1 1 SORT 3.6 2023/03/24 14:14:19 1 1 MERGE 3.6 2023/03/27 17:47:52 1 1 SORT 3.6 2023/03/27 17:48:02 1 1 CLEAN 5.3 2023/03/27 17:48:18 1 1 HDREDIT2 3.2 2023/03/27 17:49:33 ? ? HDREDIT2 3.2 2023/03/27 17:52:18 ? ? CLEAN 5.3 2023/03/31 16:28:46 1 1 $END $REMARKS -CLEAN functions: 2020/07/30 15:37:27 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Set event to last 4 characters of file name Set header Start and End times from the data. -REMOVECH 2020/07/30 15:38:38 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2020/07/30 16:43:31 Sorted in ascending order of channel Pressure:CTD [decibar] -HEADER EDITS: 2020/07/30 16:50:43 Applied edit header: D:\Telework\2020-047\Processing\doc\HYDRO\2020-047-bot-hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F9.4 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 6: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs Channel 7: Oxygen:Dissolved:SBE [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Channel 8: Oxygen:Dissolved:SBE [umol/kg] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE -CLEAN functions: 2021/04/14 13:41:43 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Set event to last 4 characters of file name -SORT parameters: 2023/03/24 14:14:19 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/27 17:47:52 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2020-047\Processing\IOS\2020-047-0007.che1 Secondary file: C:\HPLC\2020-047\Processing\IOS\2020-047-0007.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/27 17:48:02 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/27 17:48:18 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 17:49:33 Applied edit header: C:\HPLC\2020-047\2020-047-hdr1.txt -HEADER EDITS: 2023/03/27 17:52:18 Applied edit header: C:\HPLC\2020-047\2020-047-hdr2.txt Channel 21: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/31 16:28:46 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in cryovials in liquid nitro Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a standa Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~1 week after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Chlorophyll values ranged from 0.52 to 3.47ug/l in 17 samples. Average %CV for this cruise was 33.1 with 4 out 5 duplicates having a CV>10% and 2 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is poor. For details see worksheet “CV%” in file 2020-047 CHL QF*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Peña, 2014. Analysis was performed ~3 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2020-047HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 21 - 24 days after collection. For details, see document QF2020-047SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. All analysis was performed on frozen samples. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document NUTS_QF2020-047.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 3. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 4. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- Oxygen:Dissolved, Conductivity and Fluorescence:URU:Wetlabs data are nominal and unedited except that some records were removed in editing temperature and salinity and some obviously bad deep fluorescence values were padded in file 2020-047-0006.ctd. CTD salinity values were higher than the bottle samples by an average of 0.0035psu. Most of that difference is likely due to incomplete flushing of Niskin bottles so that samples represent conditions higher in the water column where salinity is lower. There were only 4 calibration samples included in the comparison with CTD salinity. No recalibration was applied to the CTD salinity. Oxygen:Dissolved:SBE values cannot be confirmed as there was no calibration sampling for dissolved oxygen. The surface % saturation values appear reasonable. While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. The files for events #31-42 contain upcast data due to some near-surface instrumental problems. Note that CTD data to accompany the surface bottles were selected from the shallowest data that looked reliable. For details on the processing see document: 2020-047_Processing_Report.doc. !-1-- --2--- --3--- ---4---- ---5---- ---6--- --7--- --8-- ----9---- ---10--- 11 --12-- 13 --14-- 15 ---16-- 17 --18-- 19 --20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- 46 !Samp Pressu Depth Temperat Salinity Fluores Oxygen Oxyge Conductiv Salinity Fl Nitrat Fl Silica Fl Phospha Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re ure: :T0:C0 cence: : n: ity: :Bottle ag e_ ag te ag te ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb Primary URU: Dissol Disso Primary ~o plus_ ~t ~i ~p Extrac ~a t: a chlide fuco fuco : !er Wetlabs ved: lved: tt Nitrit ri ca ha ted ct Extrac HP ! SBE SBE le e te te te ed ted LC !---- ------ ------ -------- -------- ------- ------ ----- --------- -------- -- ------ -- ------ -- ------- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 3 1.1 1.1 6.5627 25.8803 2.435 7.30 319.6 2.654517 -99.0000 0 25.98 6 59.33 6 1.978 6 1.11 0 0.43 0.019 0.002 0.020 0.076 0.006 0.004 0.009 0.005 0.178 0.027 0.042 0.030 0.003 0.022 0.084 0.000 0.008 0.017 0.000 0.182 0.002 0.000 0.960 0.017 0.966 6