*2023/03/31 14:42:44.22 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2020/03/19 20:16:00.000 NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 45 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 19 19 2 Pressure decibar 1 1 3 Depth metres 1 1 4 Temperature:Primary 'deg C (ITS90)' 7.7359 7.7359 5 Salinity:T0:C0 PSS-78 29.2216 29.2216 6 Oxygen:Dissolved:SBE mL/L 6.76 6.76 7 Oxygen:Dissolved:SBE umol/kg 295.1 295.1 8 Fluorescence:URU:Wetlabs mg/m^3 2.605 2.605 9 Salinity:Bottle PSS-78 -99 -99 10 Flag:Salinity:Bottle ' ' 11 Nitrate_plus_Nitrite umol/L 18.87 18.87 12 Flag:Nitrate_plus_Nitrite ' ' 13 Silicate umol/L 38.72 38.72 14 Flag:Silicate ' ' 15 Phosphate umol/L 1.711 1.711 16 Flag:Phosphate ' ' 17 Chlorophyll:Extracted mg/m^3 6.09 6.09 18 Flag:Chlorophyll:Extracted ' ' 19 Phaeo-Pigment:Extracted mg/m^3 1.31 1.31 20 HPLC:Chl-c3 mg/m^3 0.133 0.133 21 HPLC:Chlide-a mg/m^3 0.7E-01 0.7E-01 22 HPLC:MgDVP mg/m^3 0.3E-01 0.3E-01 23 HPLC:Chl-c2 mg/m^3 0.533 0.533 24 HPLC:Chl-c1 mg/m^3 0.381 0.381 25 HPLC:Me-chlide mg/m^3 0.359 0.359 26 HPLC:Peri mg/m^3 0.25E-01 0.25E-01 27 HPLC:But-fuco mg/m^3 0.26E-01 0.26E-01 28 HPLC:Fuco mg/m^3 2.961 2.961 29 HPLC:Neo mg/m^3 0.8E-02 0.8E-02 30 HPLC:Pras mg/m^3 0 0 31 HPLC:Viola mg/m^3 0.5E-02 0.5E-02 32 HPLC:Hex-fuco mg/m^3 0 0 33 HPLC:Diadino mg/m^3 0.219 0.219 34 HPLC:Allo mg/m^3 0.19E-01 0.19E-01 35 HPLC:Diato mg/m^3 0.4E-01 0.4E-01 36 HPLC:Zea mg/m^3 0.6E-02 0.6E-02 37 HPLC:Lut mg/m^3 0 0 38 HPLC:Gyr-de mg/m^3 0 0 39 HPLC:Chl-b mg/m^3 0.19E-01 0.19E-01 40 HPLC:c2-MGDG mg/m^3 0.26E-01 0.26E-01 41 HPLC:DVChl-a mg/m^3 0 0 42 HPLC:Chl-a mg/m^3 4.215 4.215 43 HPLC:B-Car mg/m^3 0.54E-01 0.54E-01 44 HPLC:TChl-a mg/m^3 4.644 4.644 45 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 9 F R4 4 5 -99 ' ' 9 F R4 4 6 -99 ' ' 7 F R4 2 7 -99 ' ' 6 F ' ' 1 8 -99 ' ' 8 F R4 3 9 -99 ' ' 9 F R4 4 10 -99 ' ' 3 NQ C ' ' 11 -99 ' ' 7 F R4 2 12 -99 ' ' 3 NQ C ' ' 13 -99 ' ' 7 F R4 2 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 8 F R4 3 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 7 F R4 2 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2020-007 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 56 EVENT NUMBER : 53 LATITUDE : 48 46.40400 N ! (deg min) LONGITUDE : 123 1.60800 W ! (deg min) WATER DEPTH : 210 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0573 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.3.1 2020/08/11 16:06:28 1 1 CLEAN 5.2.4 2020/08/11 16:08:29 1 1 CHGUNITS 3.1.2 2020/08/11 16:17:53 1 1 REMOVECH 8.2 2020/08/11 16:18:29 1 1 REORDER 1.3.1 2020/08/11 16:26:52 ? ? SORT 3.6 2020/08/11 16:26:59 1 1 HDREDIT2 3.2 2020/08/11 16:39:17 ? ? SORT 3.6 2023/03/24 14:03:27 1 1 MERGE 3.6 2023/03/27 16:51:29 1 1 SORT 3.6 2023/03/27 16:51:39 1 1 CLEAN 5.3 2023/03/27 16:51:50 1 1 HDREDIT2 3.2 2023/03/27 16:52:34 ? ? HDREDIT2 3.2 2023/03/27 16:52:53 ? ? CLEAN 5.3 2023/03/31 14:42:44 1 1 $END $REMARKS -CLEAN functions: 2020/08/11 16:08:28 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Set event to last 4 characters of file name Set header Start and End times from the data. -CHANGE units: Temperature reference channel: Temperature:CTD [deg C (ITS90)] Salinity reference channel: Salinity:CTD [PSS-78] 'Oxygen:Dissolved:Volume:CTD' changed from mL/L to umol/kg -REMOVECH 2020/08/11 16:18:29 The following CHANNEL(S) were removed: Date TIME:UTC Oxygen:Dissolved:Volume:CTD [umol/kg] -SORT parameters: 2020/08/11 16:26:59 Sorted in ascending order of channel Pressure* -HEADER EDITS: 2020/08/11 16:39:17 Applied edit header: D:\Telework\2020-007\Processing\doc\HYDRO\2020-007-bot-hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F9.4 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 8: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs Channel 6: Oxygen:Dissolved:SBE [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Format: F9.4 ==> F7.2 Channel 7: Oxygen:Dissolved:SBE [umol/kg] Name: Oxygen:Dissolved:Mass:CTD ==> Oxygen:Dissolved:SBE -SORT parameters: 2023/03/24 14:03:27 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/27 16:51:29 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2020-007\Processing\IOS\2020-007-0053.che1 Secondary file: C:\HPLC\2020-007\Processing\IOS\2020-007-0053.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/27 16:51:39 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/27 16:51:50 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 16:52:34 Applied edit header: C:\HPLC\2020-007\2020-007-hdr1.txt -HEADER EDITS: 2023/03/27 16:52:53 Applied edit header: C:\HPLC\2020-007\2020-007-hdr2.txt Channel 20: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/31 14:42:43 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials sy -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~1 week after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Chlorophyll values ranged from 3.21 to 23.40ug/l in 16 samples. Average %CV for this cruise was 6.51 with 0 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is good. This data set is small, 16 samples, with only 3 duplicates pairs analyzed (1 replicate was lost for sample 17). For details see worksheet “CV%” in file 2020-007 CHL QF*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Peña, 2014. Analysis was performed ~2 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2020-007HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 6-9 days after collection. For details, see document QF2020-007SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. For samples 400m and deeper; a second set of samples were collected and stored cool for silicate analysis. All samples were returned to the Institute of Ocean Sciences. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document QF2020-007_NUTS*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 3. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 4. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- Oxygen:Dissolved, Conductivity and Fluorescence:URU:Wetlabs data are nominal and unedited except that some records were removed in editing temperature and salinity. CTD salinity values were higher than the 5 bottle samples by an average of 0.0036psu. Most of that difference is likely due to incomplete flushing of Niskin bottles so that samples represent conditions higher in the water column where salinity is lower. No recalibration was applied to the CTD salinity. Oxygen:Dissolved:SBE values cannot be confirmed as there was no calibration sampling for dissolved oxygen. The surface % saturation values appear reasonable. While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. For this cruise the CTD fluorescence data generally did compare well with extracted chlorophyll samples taken at the surface. Note that CTD data to accompany the surface bottles were selected from the shallowest data available after editing and bin-averaging. For events #13 and #32 no reliable downcast data were available above 3db so upcast data were selected. For details on data processing see document: 2020-007_Processing_Report.doc. !-1-- --2--- --3--- ---4---- ---5---- --6--- --7-- ---8--- ---9---- 10 --11-- 12 --13-- 14 ---15-- 16 --17-- 18 --19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- 45 !Samp Pressu Depth Temperat Salinity Oxygen Oxyge Fluores Salinity Fl Nitrat Fl Silica Fl Phospha Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re ure: :T0:C0 : n: cence: :Bottle ag e_ ag te ag te ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb Primary Dissol Disso URU: ~o plus_ ~t ~i ~p Extrac ~a t: a chlide fuco fuco : !er ved: lved: Wetlabs tt Nitrit ri ca ha ted ct Extrac HP ! SBE SBE le e te te te ed ted LC !---- ------ ------ -------- -------- ------ ----- ------- -------- -- ------ -- ------ -- ------- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 19 1.0 1.0 7.7359 29.2216 6.76 295.1 2.605 -99.0000 0 18.87 0 38.72 0 1.711 0 6.09 6 1.31 0.133 0.070 0.030 0.533 0.381 0.359 0.025 0.026 2.961 0.008 0.000 0.005 0.000 0.219 0.019 0.040 0.006 0.000 0.000 0.019 0.026 0.000 4.215 0.054 4.644 6