*2023/03/28 10:44:06.54 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/06/07 18:00:00.000 NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 45 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 21 21 2 Pressure decibar 0.9 0.9 3 Depth metres 0.9 0.9 4 Temperature:Primary 'deg C (ITS90)' 13.5403 13.5403 5 Salinity:T0:C0 PSS-78 29.4492 29.4492 6 Fluorescence:URU:Wetlabs mg/m^3 6.3 6.3 7 Oxygen:Dissolved:SBE mL/L 3.2 3.2 8 Oxygen:Dissolved:SBE umol/kg 275.2 275.2 9 Conductivity:Primary S/m 3.553001 3.553001 10 Salinity:Bottle PSS-78 -99 -99 11 Flag:Salinity:Bottle ' ' 12 Nitrate_plus_Nitrite umol/L 7.13 7.13 13 Flag:Nitrate_plus_Nitrite ' ' 14 Silicate umol/L 26.44 26.44 15 Flag:Silicate ' ' 16 Phosphate umol/L 1.145 1.145 17 Flag:Phosphate ' ' 18 Chlorophyll:Extracted mg/m^3 7.88 7.88 19 Flag:Chlorophyll:Extracted ' ' 20 HPLC:Chl-c3 mg/m^3 0.193 0.193 21 HPLC:Chlide-a mg/m^3 0.145 0.145 22 HPLC:MgDVP mg/m^3 0.6E-01 0.6E-01 23 HPLC:Chl-c2 mg/m^3 0.791 0.791 24 HPLC:Chl-c1 mg/m^3 0.509 0.509 25 HPLC:Me-chlide mg/m^3 0.21 0.21 26 HPLC:Peri mg/m^3 0.101 0.101 27 HPLC:But-fuco mg/m^3 0.58E-01 0.58E-01 28 HPLC:Fuco mg/m^3 2.691 2.691 29 HPLC:Neo mg/m^3 0.73E-01 0.73E-01 30 HPLC:Pras mg/m^3 0.15E-01 0.15E-01 31 HPLC:Viola mg/m^3 0.26E-01 0.26E-01 32 HPLC:Hex-fuco mg/m^3 0.376 0.376 33 HPLC:Diadino mg/m^3 0.611 0.611 34 HPLC:Allo mg/m^3 0.113 0.113 35 HPLC:Diato mg/m^3 0.183 0.183 36 HPLC:Zea mg/m^3 0.23E-01 0.23E-01 37 HPLC:Lut mg/m^3 0.8E-02 0.8E-02 38 HPLC:Gyr-de mg/m^3 0.15E-01 0.15E-01 39 HPLC:Chl-b mg/m^3 0.192 0.192 40 HPLC:c2-MGDG mg/m^3 0.2E-01 0.2E-01 41 HPLC:DVChl-a mg/m^3 0 0 42 HPLC:Chl-a mg/m^3 7.777 7.777 43 HPLC:B-Car mg/m^3 0.13 0.13 44 HPLC:TChl-a mg/m^3 8.132 8.132 45 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 9 F R4 4 5 -99 ' ' 9 F R4 4 6 -99 ' ' 8 F R4 3 7 -99 ' ' 7 F R4 2 8 -99 ' ' 6 F R4 1 9 -99 ' ' ' ' F10.6 R4 ' ' 10 -99 ' ' 9 F R4 4 11 -99 ' ' 3 NQ C ' ' 12 -99 ' ' 7 F R4 2 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-045 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : SC-04 EVENT NUMBER : 57 LATITUDE : 48 43.50900 N ! (deg min) LONGITUDE : 123 25.81500 W ! (deg min) WATER DEPTH : 84 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0456 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.2 2019/12/02 16:04:56 1 1 CLEAN 5.2.3 2019/12/02 16:06:10 1 1 REMOVECH 8.2 2019/12/02 16:06:35 1 1 SORT 3.6 2019/12/02 16:08:54 1 1 HDREDIT2 3.1.1 2019/12/02 16:25:04 ? ? REORDER 1.3.1 2020/07/08 16:26:20 ? ? SORT 3.6 2023/03/23 12:45:04 1 1 MERGE 3.6 2023/03/26 16:07:44 1 1 SORT 3.6 2023/03/26 16:08:09 1 1 CLEAN 5.3 2023/03/26 16:08:23 1 1 HDREDIT2 3.2 2023/03/27 14:40:38 ? ? HDREDIT2 3.2 2023/03/27 14:41:00 ? ? CLEAN 5.3 2023/03/28 10:44:06 1 1 $END $REMARKS -CLEAN functions: 2019/12/02 16:06:10 20 Change character data from " " to "0" in channels Flag:* Set header Start and End times from the data. -REMOVECH 2019/12/02 16:06:35 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2019/12/02 16:08:54 Sorted in ascending order of channel Pressure:CTD [decibar] -HEADER EDITS: 2019/12/02 16:25:04 Applied edit header: Q:\OSD_DataProcessing\Cruise_Data\2019-045\Processing\doc\HYDRO\2019-045-bot- hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F9.4 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 7: Oxygen:Dissolved:SBE [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Channel 8: Oxygen:Dissolved:SBE [umol/kg] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Channel 6: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs -SORT parameters: 2023/03/23 12:45:04 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/26 16:07:44 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2019-045\Processing\IOS\2019-045-0057.che1 Secondary file: C:\HPLC\2019-045\Processing\IOS\2019-045-0057.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/26 16:08:09 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/26 16:08:23 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 14:40:38 Applied edit header: C:\HPLC\2019-045\2019-045-bot-hdr1.txt -HEADER EDITS: 2023/03/27 14:41:00 Channel 20: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:44:05 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials sy -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~2 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Chlorophyll values ranged from 0.49 to 7.88ug/l in 16 samples. Average %CV for this cruise was 3.01 with 0 out of 7 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80 % for 2013 - 2018 so the overall quality of this dataset is excellent. For details see worksheet “CV%” in file QF 2019-045 CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6 weeks after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2019-045HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 21-24 days after collection. For details, see document QF 2019-045 SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. All analysis was performed on frozen samples. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document QF 2019-045 NUTS*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Pena, M.A. 2014. Institue of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x +80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- For this cruise most bottles were deployed as separate events from the CTD casts with surface sampling being done at the same time as the CTD cast. For 5 casts a Niskin was attached above the CTD to collect near-bottom samples. The CTD data in these files were extracted from the CTD files. In the case of surface samples, the CTD data are only available from a little lower in the water column. Conductivity and Fluorescence:URU:Wetlabs data are nominal and unedited except that some records were removed in editing temperature and salinity. Salinity calibration sampling was done using a Niskin bottle mounted above the CTD during 5 casts. CTD salinity values were higher than the bottle samples by an average of 0.008psu, or if 1 outlier is excluded, by 0.005psu. Some of that difference may be due to the samples coming from higher in the water column where salinity is lower. No recalibration was applied to the CTD salinity. There was no calibration sampling for dissolved oxygen. Evidence available from use of this sensor during a previous cruise is not strong enough to enable recalibration. Warning: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. For details on the processing see document: 2019-045_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: C:\HPLC\2019-045\Processing\IOS\2019-045-0057.hplc -------------------------------------------------- Sample_Number 21: HPLC: >10%CV for TChl-a !-1-- --2--- --3--- ---4---- ---5---- ---6--- --7--- --8-- ----9---- ---10--- 11 --12-- 13 --14-- 15 ---16-- 17 --18-- 19 ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- 45 !Samp Pressu Depth Temperat Salinity Fluores Oxygen Oxyge Conductiv Salinity Fl Nitrat Fl Silica Fl Phospha Fl Chloro Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re ure: :T0:C0 cence: : n: ity: :Bottle ag e_ ag te ag te ag phyll: ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb Primary URU: Dissol Disso Primary ~o plus_ ~t ~i ~p Extrac ~a a chlide fuco fuco : !er Wetlabs ved: lved: tt Nitrit ri ca ha ted ct HP ! SBE SBE le e te te te ed LC !---- ------ ------ -------- -------- ------- ------ ----- --------- -------- -- ------ -- ------ -- ------- -- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 21 0.9 0.9 13.5403 29.4492 6.300 3.20 275.2 3.553001 -99.0000 0 7.13 0 26.44 0 1.145 0 7.88 0 0.193 0.145 0.060 0.791 0.509 0.210 0.101 0.058 2.691 0.073 0.015 0.026 0.376 0.611 0.113 0.183 0.023 0.008 0.015 0.192 0.020 0.000 7.777 0.130 8.132 36