*2023/03/28 10:37:18.50 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/09/07 12:42:31.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 11 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 5F18AD0B NUMBER OF CHANNELS : 56 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 532 542 2 Bottle_Number n/a 1 11 3 Bottle:Firing_Sequence n/a 1 11 4 Pressure decibar 2.5 395.2 5 Depth metres 2.5 391.4 6 Temperature:Primary 'deg C (ITS90)' 9.6919 10.8036 7 Conductivity:Primary S/m 3.478242 3.506828 8 Transmissivity %/metre 22.7 50.6 9 Transmissivity:Green %/metre 26.6 74.4 10 Fluorescence:URU:Seapoint mg/m^3 0.385 2.594 11 PAR uE/m^2/sec 0 0.2 12 Salinity:T0:C0 PSS-78 30.9884 31.9909 13 Oxygen:Dissolved:SBE mL/L 3.42 4.74 14 Oxygen:Dissolved:SBE umol/kg 148.8 206.6 15 Number_of_bin_records n/a 230 241 16 Temperature:Draw 'deg C (ITS90)' 10.2 11.4 17 Salinity:Bottle PSS-78 30.9871 30.9871 18 Flag:Salinity:Bottle ' ' 19 Chlorophyll:Extracted mg/m^3 0.7 6.53 20 Flag:Chlorophyll:Extracted ' ' 21 Phaeo-Pigment:Extracted mg/m^3 0.23 0.3 22 Nitrate_plus_Nitrite umol/L 18.41 25.82 23 Flag:Nitrate_plus_Nitrite ' ' 24 Silicate umol/L 36.37 46.98 25 Flag:Silicate ' ' 26 Phosphate umol/L 1.649 2.177 27 Flag:Phosphate ' ' 28 Oxygen:Dissolved mL/L 3.412 4.793 29 Oxygen:Dissolved umol/kg 148.7 209.1 30 Flag:Oxygen:Dissolved ' ' 31 HPLC:Chl-c3 mg/m^3 0.4E-01 0.4E-01 32 HPLC:Chlide-a mg/m^3 0.157 0.157 33 HPLC:MgDVP mg/m^3 0.66E-01 0.66E-01 34 HPLC:Chl-c2 mg/m^3 0.527 0.527 35 HPLC:Chl-c1 mg/m^3 0.249 0.249 36 HPLC:Me-chlide mg/m^3 0.427 0.427 37 HPLC:Peri mg/m^3 0.3E-01 0.3E-01 38 HPLC:But-fuco mg/m^3 0.15E-01 0.15E-01 39 HPLC:Fuco mg/m^3 1.825 1.825 40 HPLC:Neo mg/m^3 0.45E-01 0.45E-01 41 HPLC:Pras mg/m^3 0.25E-01 0.25E-01 42 HPLC:Viola mg/m^3 0.18E-01 0.18E-01 43 HPLC:Hex-fuco mg/m^3 0.1E-01 0.1E-01 44 HPLC:Diadino mg/m^3 0.142 0.142 45 HPLC:Allo mg/m^3 0.69E-01 0.69E-01 46 HPLC:Diato mg/m^3 0.5E-02 0.5E-02 47 HPLC:Zea mg/m^3 0.13E-01 0.13E-01 48 HPLC:Lut mg/m^3 0.4E-02 0.4E-02 49 HPLC:Gyr-de mg/m^3 0 0 50 HPLC:Chl-b mg/m^3 0.92E-01 0.92E-01 51 HPLC:c2-MGDG mg/m^3 0 0 52 HPLC:DVChl-a mg/m^3 0 0 53 HPLC:Chl-a mg/m^3 3.068 3.068 54 HPLC:B-Car mg/m^3 0.46E-01 0.46E-01 55 HPLC:TChl-a mg/m^3 3.653 3.653 56 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 10 F ' ' 6 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 6 F ' ' 1 10 -99 ' ' 8 F ' ' 3 11 -99 ' ' 7 F ' ' 1 12 -99 ' ' 9 F ' ' 4 13 -99 ' ' 7 F ' ' 2 14 -99 ' ' 6 F ' ' 1 15 -99 ' ' 5 I I 0 16 -99 ' ' 6 F R4 1 17 -99 ' ' 9 F R4 4 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 7 F R4 2 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 7 F R4 2 25 -99 ' ' 3 NQ C ' ' 26 -99 ' ' 8 F R4 3 27 -99 ' ' 3 NQ C ' ' 28 -99 ' ' 8 F R4 3 29 -99 ' ' 6 F ' ' 1 30 -99 ' ' 3 NQ C ' ' 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 8 F R4 3 56 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-023 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Sastri A. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : JS01 EVENT NUMBER : 170 LATITUDE : 50 29.92000 N ! (deg min) LONGITUDE : 126 29.35000 W ! (deg min) WATER DEPTH : 400 ALTIMETER (M) : 9.99 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 $REMARKS Software Version Seasave V 7.26.7.107 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.2.2 2020/01/28 09:29:17 2640 2640 CLEAN 5.2.3 2020/01/28 09:29:47 2640 2640 ADDSAMP 3.6 2020/01/30 14:22:53 2640 2640 BINAVE 4.2.1 2020/03/13 14:27:49 2640 11 MERGE 3.5.1 2020/03/18 11:17:52 11 11 CLEAN 5.2.4 2020/03/18 11:19:15 11 11 CALIB 12.0.1 2020/03/18 11:28:04 11 11 SORT 3.6 2020/03/18 11:28:16 11 11 REMOVECH 8.2 2020/03/18 11:28:44 11 11 CHGUNITS 3.1.2 2020/03/18 11:28:54 11 11 CHGUNITS 3.1.2 2020/03/18 11:35:35 11 11 REORDER 1.3.1 2020/03/18 11:35:47 ? ? HDREDIT2 3.2 2020/03/18 11:52:52 ? ? CALIB 12.0.1 2021/01/20 13:09:09 11 11 HDREDIT2 3.2 2021/01/20 16:10:34 ? ? HDREDIT2 3.2 2021/01/20 16:13:26 ? ? MERGE 3.6 2021/08/20 16:36:04 11 11 CHGUNITS 3.2 2021/08/20 16:52:22 11 11 REORDER 1.3.1 2021/08/20 16:54:50 ? ? SORT 3.6 2023/03/23 12:18:24 11 11 MERGE 3.6 2023/03/26 15:39:02 11 11 SORT 3.6 2023/03/26 15:39:23 11 11 CLEAN 5.3 2023/03/26 15:39:34 11 11 HDREDIT2 3.2 2023/03/26 15:40:15 ? ? HDREDIT2 3.2 2023/03/26 15:40:38 ? ? CLEAN 5.3 2023/03/28 10:37:18 11 11 $END $REMARKS -CLEAN functions: 2020/01/28 09:29:27 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\OSD_DataProcessing\Cruise_Data\2019-023\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number [n/a] Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2020/03/18 11:17:52 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Temperature:Draw, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium Primary file : D:\Telework\2019-023\Processing\hydro\2019-023-0170.samavg Secondary file: D:\Telework\2019-023\Processing\hydro\2019-023-0170.mrgcln1s Comments from secondary file: D:\Telework\2019-023\Processing\hydro\2019-023-0170.mrgcln1s ------------------------------------------------------------ -CLEAN functions: 2020/01/30 14:43:57 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2020/01/30 14:44:03 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2020/03/18 11:19:13 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -CALIB parameters: 2020/03/18 11:28:04 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = D:\Telework\2019-023\Processing\doc\2019-023-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Salinity:T0:C0 PSS-78 10 0.8200000E-02 0.1000000E+01 19 Salinity:T1:C1 PSS-78 10 -0.8000000E-03 0.1000000E+01 20 Oxygen:Dissolved:SBE mL/L 10 0.1670000E-01 0.1038400E+01 -SORT parameters: 2020/03/18 11:28:16 Sorted in ascending order of channel Press* -REMOVECH 2020/03/18 11:28:44 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 - No units changed -HEADER EDITS: 2020/03/18 11:52:52 Applied edit header: D:\Telework\2019-023\Processing\doc\HYDRO\2019-023-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F10.3 ==> F7.1 Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F10.3 ==> F7.1 Channel 6: Conductivity:Primary [S/m] Format: F11.6 ==> F10.6 Channel 8: Transmissivity:Green [%/metre] Name: Transmissivity2 ==> Transmissivity:Green Channel 10: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 12: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 18: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 20: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 21: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 23: Silicate [umol/L] Units: ==> umol/L Channel 25: Phosphate [umol/L] Units: ==> umol/L Channel 16: Salinity:Bottle [PSS-78] Units: ==> PSS-78 -CALIB parameters: 2021/01/20 13:09:09 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = 2019-023-transcor.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 8 Transmissivity:Green %/metre 10 -0.2000000E-01 0.1439800E+01 -HEADER EDITS: 2021/01/20 16:10:34 Channel 1: Bottle_Number [n/a] Format: I3 ==> I5 Channel 2: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 -HEADER EDITS: 2021/01/20 16:13:26 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2019\2019-023-transmissivity\ 2019-023-trms-hdr1.txt -The following MERGE parameters were used: 2021/08/20 16:36:04 Merge Channel: Sample_Number Merge Scheme Used: 2: Add Secondary to Closest Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw Primary file : D:\Telework\2019-023\2019-023-0170.cheold Secondary file: D:\Telework\2019-023\2019-023-0170.ios2 11 secondary records matched to primary records. -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Sal* 'Oxygen:Dissolved [mL/L]' changed from mL/L to umol/kg -SORT parameters: 2023/03/23 12:18:24 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/26 15:39:02 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2019-023\Processing\IOS\2019-023-0170.che1 Secondary file: C:\HPLC\2019-023\Processing\IOS\2019-023-0170.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/26 15:39:23 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/26 15:39:33 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/26 15:40:15 Applied edit header: C:\HPLC\2019-023\2019-023-bot-hdr1.txt -HEADER EDITS: 2023/03/26 15:40:38 Applied edit header: C:\HPLC\2019-023\2019-023-bot-hdr2.txt Channel 2: Bottle_Number [n/a] Format: I5 ==> I3 Channel 3: Bottle:Firing_Sequence [n/a] Format: I5 ==> I3 Channel 31: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 55: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:37:16 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS SBE HEADER Sea-Bird SBE 9 Data File: FileName = C:\CTD Data\2019-023\2019-023-0170.hex Software Version Seasave V 7.26.7.107 Temperature SN = 2023 Conductivity SN = 1763 Number of Bytes Per Scan = 37 Number of Voltage Words = 4 Number of Scans Averaged by the Deck Unit = 1 System UpLoad Time = Sep 07 2019 12:44:36 NMEA Latitude = 50 29.92 N NMEA Longitude = 126 29.35 W NMEA UTC (Time) = Sep 07 2019 12:42:31 Store Lat/Lon Data = Append to Every Scan SBE 11plus V 5.1e number of scans to average = 1 pressure baud rate = 9600 NMEA baud rate = 4800 GPIB address = 1 advance primary conductivity 0.073 seconds advance secondary conductivity 0.073 seconds S> Tully: 2019-023 Station: JS01 Depth: 400 System UTC = Sep 07 2019 12:44:36 # nquan = 21 # nvalues = 2640 # units = specified # name 0 = scan: Scan Count # name 1 = bpos: Bottle Position in Carousel # name 2 = nbf: Bottles Fired # name 3 = prDM: Pressure, Digiquartz [db] # name 4 = depSM: Depth [salt water, m] # name 5 = t090C: Temperature [ITS-90, deg C] # name 6 = t190C: Temperature, 2 [ITS-90, deg C] # name 7 = c0S/m: Conductivity [S/m] # name 8 = c1S/m: Conductivity, 2 [S/m] # name 9 = CStarTr0: Beam Transmission, WET Labs C-Star [%] # name 10 = CStarTr1: Beam Transmission, WET Labs C-Star, 2 [%] # name 11 = flSP: Fluorescence, Seapoint # name 12 = sbeox0V: Oxygen raw, SBE 43 [V] # name 13 = par: PAR/Irradiance, Biospherical/Licor # name 14 = altM: Altimeter [m] # name 15 = dz/dtM: Descent Rate [m/s] # name 16 = pumps: Pump Status # name 17 = sal00: Salinity, Practical [PSU] # name 18 = sal11: Salinity, Practical, 2 [PSU] # name 19 = sbeox0ML/L: Oxygen, SBE 43 [ml/l] # name 20 = flag: 0.000e+00 # interval = seconds: 0.0416667 # start_time = Sep 07 2019 12:42:31 [NMEA time, header] # bad_flag = -9.990e-29 # datcnv_date = Jan 27 2020 16:29:41, 7.26.7.129 # datcnv_in = P:\Cruise_Data_Raw\2019\2019-023\CTD Data\2019-023-0170.hex Q:\OSD _DataProcessing\Cruise_Data\2019-023\Processing\doc\2019-023-ctd.XMLCON # datcnv_ox_hysteresis_correction = yes # datcnv_ox_tau_correction = yes # datcnv_bottle_scan_range_source = BL file # datcnv_scans_per_bottle = 241 END* Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~7 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.05-17.56ug/l in 302 samples. Average %CV for this cruise was 3.27% with 2 out of 61 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is very good. For details see worksheet “CV%” in file QF2019-023CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2019-023HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a precision analysis, see document QF2019-023OXY*.xlsx. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 8 - 19 days after collection. For details including a duplicate analysis, see document QF2019-023SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2019-023nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. Standards are made in LNSW collected at 250m at station LB14. For full details including a precision study see file 2019-023NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Conductivity, Transmissivity, Fluorescence:URU:Seapoint and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2019-023_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: D:\Telework\2019-023\2019-023-0170.ios2 --------------------------------------------------------------------- Sample_Number 540: OXY-1: Poor curve. Sample over-titrated. Sample_Number 532: OXY: Sample over-titrated but voltage off scale. Verified endpoint position was ok using O2check function. Comments from secondary file: C:\HPLC\2019-023\Processing\IOS\2019-023-0170.hplc -------------------------------------------------- Sample_Number 542: HPLC: >10%CV for TChl-a, value low compared to Turner chl a, likely underestimate *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.82E-02 1) Salinity:T1:C1 PSS-78 10 -99 () (-0.8E-03 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.167E-01 1.0384) Transmissivity:Green %/metre 10 -99 () (-0.2E-01 1.4398) $END !-1-- 2- 3- --4--- --5--- ---6---- ----7---- --8-- --9-- ---10-- --11-- ---12--- --13-- --14- -15- --16- ---17--- 18 --19-- 20 --21-- --22-- 23 --24-- 25 ---26-- 27 ---28-- --29- 30 ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- ---55-- 56 !Samp Bo Bo Pressu Depth Temperat Conductiv Trans Trans Fluores PAR Salinity Oxygen Oxyge Numb Tempe Salinity Fl Chloro Fl Phaeo- Nitrat Fl Silica Fl Phospha Fl Oxygen: Oxyge Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: ity: missi missi cence: :T0:C0 : n: er_o ratur :Bottle ag phyll: ag Pigmen e_ ag te ag te ag Dissolv n: ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Primary Primary vity vity: URU: Dissol Disso ~bin e: ~o Extrac ~a t: plus_ ~t ~i ~p ed Disso ~o a chlide fuco fuco : !er mb en Green Seapoin ved: lved: _rec Draw tt ted ct Extrac Nitrit ri ca ha lved lv HP ! er ce t SBE SBE ords le ed ted e te te te ed LC !---- -- -- ------ ------ -------- --------- ----- ----- ------- ------ -------- ------ ----- ---- ----- -------- -- ------ -- ------ ------ -- ------ -- ------- -- ------- ----- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 542 11 11 2.5 2.5 10.8036 3.481914 39.5 54.3 2.594 0.2 30.9884 4.74 206.6 230 11.4 -99.0000 0 6.53 0 0.25 18.41 0 36.37 0 1.649 0 4.793 209.1 0 0.040 0.157 0.066 0.527 0.249 0.427 0.030 0.015 1.825 0.045 0.025 0.018 0.010 0.142 0.069 0.005 0.013 0.004 0.000 0.092 0.000 0.000 3.068 0.046 3.653 46 541 10 10 4.9 4.9 10.7243 3.491010 43.1 61.2 1.699 0.0 31.1447 4.54 198.2 241 11.3 30.9871 0 2.89 0 0.27 22.83 0 42.31 0 1.929 0 4.518 197.1 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 540 9 9 10.1 10.0 10.7604 3.506828 46.2 66.5 0.987 0.0 31.2683 4.38 191.2 241 11.1 -99.0000 0 1.48 0 0.23 24.29 0 41.71 0 1.999 0 4.244 185.1 26 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 539 8 8 20.1 19.9 10.3539 3.480784 48.3 70.0 0.742 0.0 31.3540 4.01 175.0 241 10.9 -99.0000 0 0.84 6 0.24 25.01 6 44.43 6 1.999 6 3.988 173.9 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 538 7 7 30.3 30.0 10.2945 3.478242 48.6 70.4 0.654 0.0 31.3756 3.94 171.6 241 10.8 -99.0000 0 0.70 0 0.30 24.96 0 44.33 0 2.027 0 3.920 171.0 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 537 6 6 50.1 49.7 10.1728 3.479616 49.2 71.3 0.509 0.0 31.4865 3.84 167.4 241 10.7 -99.0000 0 -99.00 0 -99.00 25.18 0 43.86 0 1.997 0 3.808 166.0 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 536 5 5 75.4 74.7 9.9903 3.481573 49.3 71.4 0.425 0.0 31.6545 3.70 161.3 241 10.5 -99.0000 0 -99.00 0 -99.00 25.41 0 42.61 0 1.959 0 3.676 160.3 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 535 4 4 100.0 99.1 9.8704 3.484036 50.6 74.4 0.385 0.0 31.7742 3.62 157.7 241 10.4 -99.0000 0 -99.00 0 -99.00 25.54 0 43.99 0 2.046 0 3.604 157.1 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 534 3 3 150.1 148.7 9.8025 3.488550 49.6 72.7 0.452 0.0 31.8583 3.58 155.9 241 10.3 -99.0000 0 -99.00 0 -99.00 25.58 0 43.60 0 2.005 0 3.564 155.4 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 533 2 2 200.2 198.4 9.7346 3.491438 49.3 72.4 0.460 0.0 31.9263 3.53 153.8 241 10.3 -99.0000 0 -99.00 0 -99.00 25.82 0 44.24 0 2.085 0 3.524 153.6 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 532 1 1 395.2 391.4 9.6919 3.502223 22.7 26.6 0.525 0.0 31.9909 3.42 148.8 241 10.2 -99.0000 0 -99.00 0 -99.00 25.79 0 46.98 0 2.177 0 3.412 148.7 2 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0