*2024/09/05 11:29:00.58 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/08/22 18:33:30.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 16 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 289AB2D9 NUMBER OF CHANNELS : 55 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 695 710 2 Bottle_Number n/a 1 16 3 Bottle:Firing_Sequence n/a 1 16 4 Pressure decibar 3.8 400.4 5 Depth metres 3.8 396.6 6 Temperature:Primary 'deg C (ITS90)' 4.2826 15.2972 7 Transmissivity %/metre 36.6 48.9 8 Fluorescence:URU:Seapoint mg/m^3 0.124 2.738 9 Salinity:T0:C0 PSS-78 32.334 33.9567 10 Oxygen:Dissolved:SBE mL/L 1.44 6.83 11 Oxygen:Dissolved:SBE umol/kg 62.7 297.5 12 Conductivity:Primary S/m 3.209614 4.024464 13 Number_of_bin_records n/a 155 241 14 Nitrate_plus_Nitrite umol/L 0 0 15 Flag:Nitrate_plus_Nitrite ' ' 16 Silicate umol/L 0.73 0.73 17 Flag:Silicate ' ' 18 Phosphate umol/L 0.238 0.238 19 Flag:Phosphate ' ' 20 Chlorophyll:Extracted mg/m^3 0.98 0.98 21 Flag:Chlorophyll:Extracted ' ' 22 Phaeo-Pigment:Extracted mg/m^3 0.7E-01 0.7E-01 23 Transmissivity:Green %/metre 50.4 71.3 24 HPLC:Chl-c3 mg/m^3 0.48E-01 0.48E-01 25 HPLC:Chlide-a mg/m^3 0.8E-02 0.8E-02 26 HPLC:MgDVP mg/m^3 0.4E-02 0.4E-02 27 HPLC:Chl-c2 mg/m^3 0.157 0.157 28 HPLC:Chl-c1 mg/m^3 0.14E-01 0.14E-01 29 HPLC:Me-chlide mg/m^3 0.15E-01 0.15E-01 30 HPLC:Peri mg/m^3 0.12E-01 0.12E-01 31 HPLC:But-fuco mg/m^3 0.25E-01 0.25E-01 32 HPLC:Fuco mg/m^3 0.442 0.442 33 HPLC:Neo mg/m^3 0.15E-01 0.15E-01 34 HPLC:Pras mg/m^3 0 0 35 HPLC:Viola mg/m^3 0.2E-02 0.2E-02 36 HPLC:Hex-fuco mg/m^3 0.168 0.168 37 HPLC:Diadino mg/m^3 0.142 0.142 38 HPLC:Allo mg/m^3 0 0 39 HPLC:Diato mg/m^3 0.18E-01 0.18E-01 40 HPLC:Zea mg/m^3 0.4E-02 0.4E-02 41 HPLC:Lut mg/m^3 0.4E-02 0.4E-02 42 HPLC:Gyr-de mg/m^3 0.4E-02 0.4E-02 43 HPLC:Chl-b mg/m^3 0.45E-01 0.45E-01 44 HPLC:c2-MGDG mg/m^3 0.4E-02 0.4E-02 45 HPLC:DVChl-a mg/m^3 0 0 46 HPLC:Chl-a mg/m^3 0.849 0.849 47 HPLC:B-Car mg/m^3 0.12E-01 0.12E-01 48 HPLC:TChl-a mg/m^3 0.872 0.872 49 Flag:HPLC n/a 50 Carbon:Dissolved:Organic uM 32 76.6 51 Flag:Carbon:Dissolved:Organic n/a 52 Carbon:Total:Organic uM 49.5 81.3 53 Flag:Carbon:Total:Organic n/a 54 CDOM:Available n/a 1 1 55 Flag:CDOM:Available n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 6 F ' ' 1 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 10 F ' ' 6 13 -99 ' ' 5 I I 0 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 8 F R4 3 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 6 F ' ' 1 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 3 NQ C ' ' 50 -99 ' ' 7 F R4 2 51 -99 ' ' 3 NQ C ' ' 52 -99 ' ' 7 F R4 2 53 -99 ' ' 3 NQ C ' ' 54 -99 ' ' 3 I I 0 55 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-008 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P25 EVENT NUMBER : 88 LATITUDE : 49 59.97000 N ! (deg min) LONGITUDE : 143 36.27000 W ! (deg min) WATER DEPTH : 4123 ALTIMETER (M) : 10.79 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 2023 Conductivity ' ' 1763 'Pressure:Digiquartz with TC' ' ' 0506 Temperature:2 ' ' 5013 Conductivity:2 ' ' 3394 'Oxygen:SBE 43' ' ' 3234 Fluorometer:Seapoint ' ' 3640 'Transmissometer:WET Labs C-Star' ' ' 1185DR 'Transmissometer:WET Labs C-Star:2' ' ' 1883DG PAR/Irradiance:Biospherical/Licor ' ' 70613 Altimeter ' ' 62355 $END $REMARKS Software Version Seasave V 7.26.7.107 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.2.2 2020/01/24 08:25:20 3715 3715 CLEAN 5.2.3 2020/01/24 08:37:07 3715 3715 ADDSAMP 3.6 2020/01/24 11:27:59 3715 3715 BINAVE 4.2 2020/01/24 11:30:03 3715 16 MERGE 3.5.1 2020/02/13 14:04:46 16 16 CLEAN 5.2.4 2020/02/24 15:59:44 16 16 CALIB 12.0.1 2020/02/24 16:00:05 16 16 SORT 3.6 2020/02/24 16:00:09 16 16 REMOVECH 8.2 2020/02/24 16:00:46 16 16 CHGUNITS 3.1.2 2020/02/24 16:03:54 16 16 CHGUNITS 3.1.2 2020/02/24 16:04:18 16 16 REORDER 1.3.1 2020/03/17 12:59:09 ? ? REMOVECH 8.2 2020/03/18 10:46:38 16 16 SORT 3.6 2020/03/18 10:54:55 16 16 HDREDIT2 3.2 2020/03/18 10:55:31 ? ? CALIB 12.0.1 2021/01/20 12:16:01 16 16 SORT 3.6 2023/03/12 19:35:38 16 16 REORDER 1.3.1 2023/03/12 19:36:18 ? ? MERGE 3.6 2023/03/12 19:36:51 16 16 SORT 3.6 2023/03/12 19:37:37 16 16 CLEAN 5.3 2023/03/12 19:37:53 16 16 HDREDIT2 3.2 2023/03/19 15:05:51 ? ? REMOVECH 8.2 2023/03/19 15:06:19 16 16 HDREDIT2 3.2 2023/03/19 15:06:46 ? ? CLEAN 5.3 2023/03/28 10:35:15 16 16 SORT 3.6 2024/09/05 11:04:09 16 16 MERGE 3.6 2024/09/05 11:12:07 16 16 SORT 3.6 2024/09/05 11:16:19 16 16 CLEAN 5.3 2024/09/05 11:16:33 16 16 HDREDIT2 3.2 2024/09/05 11:28:30 ? ? $END $REMARKS -CLEAN functions: 2020/01/24 08:36:48 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\OSD_DataProcessing\Cruise_Data\2019-008\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2020/02/13 14:04:46 Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide, Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolved, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total, HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC, Salinity:Bottle, Flag:Salinity:Bottle, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw Primary file : Q:\OSD_DataProcessing\Cruise_Data\2019-008\Processing\hydro\2019-008-0088.samavg Secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-008\Processing\hydro\2019-008-0088.mrgcln1s Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-008\Processing\hydro\2019-008-0088.mrgcln1s --------------------------------------------------------------------------- -SORT parameters: 2020/02/13 14:01:41 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2020/02/24 15:59:42 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Remove Sea-Bird comments from the header. -CALIB parameters: 2020/02/24 16:00:05 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\OSD_DataProcessing\Cruise_Data\2019-008\Processing\doc\2019-008-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Salinity:T0:C0 PSS-78 10 0.8300000E-02 0.1000000E+01 20 Salinity:T1:C1 PSS-78 10 0.4000000E-03 0.1000000E+01 21 Oxygen:Dissolved:SBE mL/L 10 0.1820000E-01 0.1034800E+01 -SORT parameters: 2020/02/24 16:00:09 Sorted in ascending order of channel Press* -REMOVECH 2020/02/24 16:00:46 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 - No units changed -REMOVECH 2020/03/18 10:46:38 The following CHANNEL(S) were removed: HPLC:Chl-c3 HPLC:Chlide-a HPLC:MgDVP HPLC:Chl-c2 HPLC:Chl-c1 HPLC:Me-chlide HPLC:Peri HPLC:But-fuco HPLC:Fuco HPLC:Neo HPLC:Pras HPLC:Viola HPLC:Hex-fuco HPLC:Diadino HPLC:Allo HPLC:Diato HPLC:Zea HPLC:Lut HPLC:Gyr-de HPLC:Chl-b HPLC:c2-MGDG HPLC:DVChl-a HPLC:Chl-a HPLC:B-Car HPLC:TChl-a Flag:HPLC -SORT parameters: 2020/03/18 10:54:55 Sorted in ascending order of channel Press* -HEADER EDITS: 2020/03/18 10:55:31 Applied edit header: D:\Telework\2019-008\Processing\doc\HYDRO\2019-008-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F10.3 ==> F7.1 Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F10.3 ==> F7.1 Channel 6: Conductivity:Primary [S/m] Format: F11.6 ==> F10.6 Channel 8: Transmissivity:Green [%/metre] Name: Transmissivity2 ==> Transmissivity:Green Channel 11: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 15: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 17: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 18: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 20: Silicate [umol/L] Units: ==> umol/L Channel 22: Phosphate [umol/L] Units: ==> umol/L -CALIB parameters: 2021/01/20 12:16:01 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = 2019-008-transcor.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 8 Transmissivity:Green %/metre 10 -0.2000000E-01 0.1439800E+01 -SORT parameters: 2023/03/12 19:35:38 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/12 19:36:51 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-008\Processing\IOS\2019-008-0088. che2 Secondary file: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-008\Processing\IOS\2019-008-0088. hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/12 19:37:37 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/12 19:37:52 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/19 15:05:51 - none. -REMOVECH 2023/03/19 15:06:19 The following CHANNEL(S) were removed: HPLC:B-Car -HEADER EDITS: 2023/03/19 15:06:46 Channel 24: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:35:13 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -SORT parameters: 2024/09/05 11:04:09 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2024/09/05 11:12:07 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Carbon:Dissolved:Organic, Flag:Carbon:Dissolved:Organic, Carbon:Total:Organic, Flag:Carbon:Total:Organic, CDOM:Available, Flag:CDOM:Available Primary file : C:\Users\huntingtons\Desktop\Additions\Carbon Additions\2019-008\Processing\IOS\ 2019-008-0088.srt Secondary file: C:\Users\huntingtons\Desktop\Additions\Carbon Additions\2019-008\Processing\IOS\ 2019-008-0088.car 14 secondary records matched to primary records. -SORT parameters: 2024/09/05 11:16:19 Sorted in ascending order of channel Pressure -CLEAN functions: 2024/09/05 11:16:32 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2024/09/05 11:28:30 Applied edit header: C:\Users\huntingtons\Desktop\Additions\Carbon Additions\2019-008\Processing\ DOC\2019-008_header1.txt -HEADER EDITS: 2024/09/05 11:29:00 Applied edit header: C:\Users\huntingtons\Desktop\Additions\Carbon Additions\2019-008\Processing\ DOC\2019-008_header2.txt $END *COMMENTS Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~2 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.03-10.66ug/l in 115 samples. Average %CV for this cruise was 2.80% with 0 out of 35 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is excellent. For details see worksheet “CV%” in file QF2019-008CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF2019-008_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2019-008OXY*.xlsx. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 13 - 25 days after collection. For details including a duplicate analysis, see document QF2019-008SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2019-008nuts*.xlsx. DMS samples were collected in 250mL ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100mL/min. The DMS was extracted from the water and absorbed into a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a dewar containing boiling water) onto a Chromosil 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/L, so “0” values should be interpreted as <0.1nmol/L. DMS data are limited to 2 significant figures. Unless otherwise indicated the % relative difference between duplicates is <20%. For more detail see file "2019-008 DMS report.doc" and for data and duplicate analysis see "QF2019-008_DMS*.xlsx." DMSP-D: Approximately 20-75mL of seawater was allowed to flow directly from the niskin into a magnetic filtration funnel containing a 0.7um GF/F filter. The first 3.5mL was collected in a polypropylene tube (15mL) containing 50uL of a 50% sulphuric acid solution. A new funnel and filter are used for each sample. Samples are hydrolized and run at IOS when time permits. DMPS-T: 3.5mL of seawater was collected directly from the niskin into a polypropylene tube (15mL) containing 50uL of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples are stored in the dark at 4 degrees C for a minimum of 24 hours. They are hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. DMSP data are limited to 2 significant figures. Unless otherwise indicated the % relative difference between duplicates is <20%. For details on analysis and raw data, see document "QF2019-008 dmsp*.xlsx. DOC and TOC samples were collected in 80 mL polycarbonate bottles and frozen until analysis. DOC samples were filtered inline from the Niskin bottles, using acid-washed filter holders and tubing and pre-combusted Whatman 25 mm GF/C filters (~1.2 ?m pore size). For analysis, samples were thawed and acidified with 4M HCl at a ratio of 100 uL HCL to 40 mL seawater. DOC samples were collected for depths <250 meters, and TOC/TDN samples were collected for depths >250 meters. For full details on analysis and processing see: Johannessen, S., Fassbender, A. J., Long, J. S., Wright, C. (2021) DOC and TOC of seawater collected during CCGS John P. Tully cruises in the northeast Pacific Ocean from Vancouver Island to Station P from 2018 to 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://www.bco-dmo.org/dataset/865829 Non-OSD researchers needing access to the data should send a request to: DFO.PACSCIIOSData-DonneesISOSCIPAC.MPO@dfo-mpo.gc.ca The following samples were collected on Cruise 2019-008. These were collected as part of the EXPORTS Program, in collaboration with Andrea Fassbender (NOAA). Cruise log and sampling logs are available upon request. Water samples for CDOM analysis were passed through a Millipore Opticap XL Durapore 0.22 µm inline filter cartridge (Product No. KVGLA04HH3) into 125 ml amber glass bottles, directly from the original Niskin bottles. Samples were refrigerated and returned to the Institute of Ocean Sciences, where they were analyzed within one year of collection. Spectral absorbance (280-700 nm at 1nm resolution) was measured with a HP Agilent 8453 spectrophotometer, using a quartz cell with a 10-cm pathlength. Absorbance, ACDOM (dimensionless), was converted to the absorption coefficient, aCDOM (m-1): aCDOM(l) = ACDOM(l)x2.303/pathlength, where l is wavelength (See Johannessen and Miller, 2001 and Stedmon et al., 2000). Spectral absorption was measured against a distilled water blank, but no offset correction was applied. Cruise logs, sampling logs and other data products from this cruise are available electronically on request from the IOS data archive (http://www-sci.pac.dfo-mpo.gc.ca/osap/data/default_e.htm) or from the authors. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. 7. Johannessen, S. C. and Miller, W. L. 2001. Quantum yield for the photochemical production of dissolved inorganic carbon in seawater. Marine Chemistry 76: 271-283. 8. Stedmon, C.A., Markager, S., Kaas, H. 2000. Optical properties and signatures of chromophoric dissolved organic matter (CDOM) in Danish coastal waters. Est. Coast. Shelf Sci. 51:567-578. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Conductivity, Transmissivity, Fluorescence:URU:Seapoint and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2019-008_Processing_Report.doc. --------------------------------------------------------------------------------- . Comments from secondary file: C:\Users\huntingtons\Desktop\Additions\Carbon Additions\2019-008\Processing\IOS\2019-008-0088.car --------------------------------------------------------------------------- Sample_Number 698: TOC: Not reported Sample_Number 703: DOC: Average of triplicate; TOC: Was supposed to be a triplicate but only two samples taken. Sample_Number 705: DOC: Was supposed to be a triplicate but only two samples taken; TOC: Average or triplicate Sample_Number 710: DOC & TOC: Average of triplicate *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.83E-02 1) Salinity:T1:C1 PSS-78 10 -99 () (0.4E-03 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.182E-01 1.0348) Transmissivity:Green %/metre 10 -99 () (-0.2E-01 1.4398) $END !-1-- 2- 3- --4--- --5--- ---6---- --7-- ---8--- ---9---- --10-- --11- ----12--- -13- --14-- 15 --16-- 17 ---18-- 19 --20-- 21 --22-- --23- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- 49 --50-- 51 --52-- 53 54 55 !Samp Bo Bo Pressu Depth Temperat Trans Fluores Salinity Oxygen Oxyge Conductiv Numb Nitrat Fl Silica Fl Phospha Fl Chloro Fl Phaeo- Trans HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl Carbon Fl Carbon Fl CD Fl !le_ tt tt re ure: missi cence: :T0:C0 : n: ity: er_o e_ ag te ag te ag phyll: ag Pigmen missi Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag : ag : ag OM ag !Numb ~u ~u Primary vity URU: Dissol Disso Primary ~bin plus_ ~t ~i ~p Extrac ~a t: vity: a chlide fuco fuco : Dissol ~g Total: ~g ~l ~l !er mb en Seapoin ved: lved: _rec Nitrit ri ca ha ted ct Extrac Green HP ved:Or an Organi an ab ab ! er ce t SBE SBE ords e te te te ed ted LC ganic ic c ic le le !---- -- -- ------ ------ -------- ----- ------- -------- ------ ----- --------- ---- ------ -- ------ -- ------- -- ------ -- ------ ----- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- ------ -- ------ -- -- -- *END OF HEADER 710 16 16 3.8 3.8 15.2939 36.8 1.021 32.3340 6.21 270.6 4.024079 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 50.7 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 71.70 6 81.30 6 1 0 708 14 14 4.9 4.9 15.2966 36.8 1.069 32.3341 6.20 270.3 4.024389 155 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 50.7 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 76.60 0 80.90 0 1 0 709 15 15 5.1 5.1 15.2972 36.8 1.071 32.3342 6.21 270.6 4.024464 241 0.00 0 0.73 0 0.238 0 0.98 6 0.07 50.7 0.048 0.008 0.004 0.157 0.014 0.015 0.012 0.025 0.442 0.015 0.000 0.002 0.168 0.142 0.000 0.018 0.004 0.004 0.004 0.045 0.004 0.000 0.849 0.012 0.872 6 -99.00 0 -99.00 0 -99 0 707 13 13 10.1 10.0 15.2853 36.8 1.705 32.3346 6.21 270.9 4.023626 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 50.5 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 73.70 0 79.30 0 1 0 705 11 11 24.9 24.7 15.1568 36.8 2.738 32.3374 6.25 272.5 4.012742 186 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 50.4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 74.30 6 77.30 6 1 0 706 12 12 25.1 24.9 15.1676 36.6 2.662 32.3369 6.24 272.0 4.013697 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 50.7 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 -99.00 0 -99.00 0 -99 0 704 10 10 50.3 49.9 9.9650 43.0 1.806 32.3876 6.83 297.5 3.550779 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 61.0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 64.50 0 67.00 0 1 0 703 9 9 75.5 74.9 7.6609 47.2 0.608 32.4029 6.71 292.3 3.351444 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 68.4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 56.90 6 61.20 6 1 0 702 8 8 100.8 99.9 6.8361 48.2 0.312 32.5152 6.39 278.1 3.291479 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 70.4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 55.20 0 61.40 0 1 0 701 7 7 125.4 124.3 6.4202 48.7 0.178 33.1135 4.84 210.6 3.310685 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 71.3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 55.60 0 55.90 0 1 0 700 6 6 150.1 148.8 5.4667 48.4 0.158 33.5172 4.33 188.3 3.264241 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 70.5 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 54.40 0 59.90 0 1 0 699 5 5 175.0 173.4 5.5357 48.3 0.150 33.7137 3.73 162.0 3.288676 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 70.2 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 51.00 0 53.20 0 1 0 698 4 4 199.7 197.9 5.3468 48.3 0.144 33.7718 3.35 145.8 3.278234 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 70.4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 51.40 0 -99.00 5 1 0 697 3 3 249.9 247.6 4.9328 48.4 0.134 33.8284 2.59 112.4 3.248964 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 70.4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 48.80 0 49.50 0 1 0 696 2 2 300.0 297.2 4.6692 48.5 0.134 33.8743 2.00 86.8 3.232007 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 70.7 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 32.00 0 52.00 0 1 0 695 1 1 400.4 396.6 4.2826 48.9 0.124 33.9567 1.44 62.7 3.209614 241 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 71.3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 49.10 0 50.20 0 1 0