*2023/03/28 10:35:14.28 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/08/16 21:45:38.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 24 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 590722A9 NUMBER OF CHANNELS : 56 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 186 209 2 Bottle_Number n/a 1 24 3 Bottle:Firing_Sequence n/a 1 24 4 Pressure decibar 5.3 2000.1 5 Depth metres 5.3 1973.7 6 Temperature:Primary 'deg C (ITS90)' 1.9612 17.2503 7 Transmissivity %/metre 42.6 57.6 8 Fluorescence:URU:Seapoint mg/m^3 0.103 2.241 9 PAR uE/m^2/sec 0 369.8 10 Salinity:T0:C0 PSS-78 32.2995 34.5861 11 Oxygen:Dissolved:SBE mL/L 0.24 7.18 12 Oxygen:Dissolved:SBE umol/kg 10.6 313 13 Conductivity:Primary S/m 3.126912 4.205132 14 Number_of_bin_records n/a 206 241 15 Temperature:Draw 'deg C (ITS90)' 4.4 18.1 16 Salinity:Bottle PSS-78 34.2817 34.5837 17 Oxygen:Dissolved mL/L 0.24 7.114 18 Oxygen:Dissolved umol/kg 10.4 310 19 Flag:Oxygen:Dissolved ' ' 20 Nitrate_plus_Nitrite umol/L 0.6 45.41 21 Flag:Nitrate_plus_Nitrite ' ' 22 Silicate umol/L 0.69 176.14 23 Flag:Silicate ' ' 24 Phosphate umol/L 0.36 3.23 25 Flag:Phosphate ' ' 26 Chlorophyll:Extracted mg/m^3 0.15 0.58 27 Flag:Chlorophyll:Extracted ' ' 28 Phaeo-Pigment:Extracted mg/m^3 0.2E-01 0.27 29 Transmissivity:Green %/metre 59.3 88.2 30 Flag:Salinity:Bottle ' ' 31 HPLC:Chl-c3 mg/m^3 0.11E-01 0.136 32 HPLC:Chlide-a mg/m^3 0 0 33 HPLC:MgDVP mg/m^3 0.1E-02 0.8E-02 34 HPLC:Chl-c2 mg/m^3 0.16E-01 0.116 35 HPLC:Chl-c1 mg/m^3 0 0 36 HPLC:Me-chlide mg/m^3 0 0 37 HPLC:Peri mg/m^3 0 0.55E-01 38 HPLC:But-fuco mg/m^3 0.14E-01 0.105 39 HPLC:Fuco mg/m^3 0.6E-02 0.74E-01 40 HPLC:Neo mg/m^3 0.2E-02 0.14E-01 41 HPLC:Pras mg/m^3 0.1E-02 0.13E-01 42 HPLC:Viola mg/m^3 0.1E-02 0.7E-02 43 HPLC:Hex-fuco mg/m^3 0.64E-01 0.376 44 HPLC:Diadino mg/m^3 0.18E-01 0.37E-01 45 HPLC:Allo mg/m^3 0 0.4E-02 46 HPLC:Diato mg/m^3 0.1E-02 0.4E-02 47 HPLC:Zea mg/m^3 0.5E-02 0.16E-01 48 HPLC:Lut mg/m^3 0.1E-02 0.9E-02 49 HPLC:Gyr-de mg/m^3 0 0.9E-02 50 HPLC:Chl-b mg/m^3 0.7E-02 0.115 51 HPLC:c2-MGDG mg/m^3 0.1E-02 0.28E-01 52 HPLC:DVChl-a mg/m^3 0 0.6E-02 53 HPLC:Chl-a mg/m^3 0.132 0.588 54 HPLC:B-Car mg/m^3 0.3E-02 0.1E-01 55 HPLC:TChl-a mg/m^3 0.132 0.591 56 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 6 F ' ' 1 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 7 F ' ' 1 10 -99 ' ' 9 F ' ' 4 11 -99 ' ' 7 F ' ' 2 12 -99 ' ' 6 F ' ' 1 13 -99 ' ' 10 F ' ' 6 14 -99 ' ' 5 I I 0 15 -99 ' ' 6 F R4 1 16 -99 ' ' 9 F R4 4 17 -99 ' ' 8 F R4 3 18 -99 ' ' 6 F ' ' 1 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 8 F R4 3 25 -99 ' ' 3 NQ C ' ' 26 -99 ' ' 7 F R4 2 27 -99 ' ' 3 NQ C ' ' 28 -99 ' ' 7 F R4 2 29 -99 ' ' 6 F ' ' 1 30 -99 ' ' 3 NQ C ' ' 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 8 F R4 3 56 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-008 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P8 EVENT NUMBER : 28 LATITUDE : 48 48.99000 N ! (deg min) LONGITUDE : 128 39.91000 W ! (deg min) WATER DEPTH : 2515 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 2023 Conductivity ' ' 1763 'Pressure:Digiquartz with TC' ' ' 0506 Temperature:2 ' ' 5013 Conductivity:2 ' ' 3394 'Oxygen:SBE 43' ' ' 3234 Fluorometer:Seapoint ' ' 3640 'Transmissometer:WET Labs C-Star' ' ' 1185DR 'Transmissometer:WET Labs C-Star:2' ' ' 1883DG PAR/Irradiance:Biospherical/Licor ' ' 70613 Altimeter ' ' 62355 $END $REMARKS Software Version Seasave V 7.26.7.107 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.2.2 2020/01/24 08:24:59 5749 5749 CLEAN 5.2.3 2020/01/24 08:36:54 5749 5749 ADDSAMP 3.6 2020/01/24 11:27:38 5749 5749 BINAVE 4.2 2020/01/24 11:29:51 5749 24 MERGE 3.5.1 2020/03/17 11:46:58 24 24 CLEAN 5.2.4 2020/03/17 11:47:01 24 24 CALIB 12.0.1 2020/03/17 11:47:16 24 24 REMOVECH 8.2 2020/03/17 11:47:37 24 24 CHGUNITS 3.1.2 2020/03/17 11:48:04 24 24 CHGUNITS 3.1.2 2020/03/17 11:48:13 24 24 REORDER 1.3.1 2020/03/17 12:59:05 ? ? REMOVECH 8.2 2020/03/18 10:46:37 24 24 SORT 3.6 2020/03/18 10:54:52 24 24 HDREDIT2 3.2 2020/03/18 10:55:29 ? ? CALIB 12.0.1 2021/01/20 12:15:59 24 24 SORT 3.6 2023/03/12 19:35:38 24 24 REORDER 1.3.1 2023/03/12 19:36:18 ? ? MERGE 3.6 2023/03/12 19:36:51 24 24 SORT 3.6 2023/03/12 19:37:37 24 24 CLEAN 5.3 2023/03/12 19:37:52 24 24 HDREDIT2 3.2 2023/03/19 15:05:51 ? ? REMOVECH 8.2 2023/03/19 15:06:19 24 24 HDREDIT2 3.2 2023/03/19 15:06:46 ? ? CLEAN 5.3 2023/03/28 10:35:14 24 24 $END $REMARKS -CLEAN functions: 2020/01/24 08:36:48 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\OSD_DataProcessing\Cruise_Data\2019-008\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2020/03/17 11:46:58 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Temperature:Draw, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide, Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolved, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total, HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : D:\Telework\2019-008\Processing\hydro\2019-008-0028.samavg Secondary file: D:\Telework\2019-008\Processing\hydro\2019-008-0028.mrgcln1s Comments from secondary file: D:\Telework\2019-008\Processing\hydro\2019-008-0028.mrgcln1s ------------------------------------------------------------ -CLEAN functions: 2020/03/17 11:46:43 20 Reduce header to sections: FIL HIS COM Remarks removed from kept sections. Remove remark heading: "Comments from secondary" -SORT parameters: 2020/03/17 11:46:47 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2020/03/17 11:47:01 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Remove Sea-Bird comments from the header. -CALIB parameters: 2020/03/17 11:47:16 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = D:\Telework\2019-008\Processing\doc\2019-008-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Salinity:T0:C0 PSS-78 10 0.8300000E-02 0.1000000E+01 19 Salinity:T1:C1 PSS-78 10 0.4000000E-03 0.1000000E+01 20 Oxygen:Dissolved:SBE mL/L 10 0.1820000E-01 0.1034800E+01 -REMOVECH 2020/03/17 11:47:37 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved' changed from mL/L to umol/kg -REMOVECH 2020/03/18 10:46:37 The following CHANNEL(S) were removed: HPLC:Chl-c3 HPLC:Chlide-a HPLC:MgDVP HPLC:Chl-c2 HPLC:Chl-c1 HPLC:Me-chlide HPLC:Peri HPLC:But-fuco HPLC:Fuco HPLC:Neo HPLC:Pras HPLC:Viola HPLC:Hex-fuco HPLC:Diadino HPLC:Allo HPLC:Diato HPLC:Zea HPLC:Lut HPLC:Gyr-de HPLC:Chl-b HPLC:c2-MGDG HPLC:DVChl-a HPLC:Chl-a HPLC:B-Car HPLC:TChl-a Flag:HPLC -SORT parameters: 2020/03/18 10:54:52 Sorted in ascending order of channel Press* -HEADER EDITS: 2020/03/18 10:55:29 Applied edit header: D:\Telework\2019-008\Processing\doc\HYDRO\2019-008-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F10.3 ==> F7.1 Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F10.3 ==> F7.1 Channel 6: Conductivity:Primary [S/m] Format: F11.6 ==> F10.6 Channel 8: Transmissivity:Green [%/metre] Name: Transmissivity2 ==> Transmissivity:Green Channel 10: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 12: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 16: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Format: F7.2 ==> F6.1 Channel 19: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 21: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 22: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 25: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 27: Silicate [umol/L] Units: ==> umol/L Channel 29: Phosphate [umol/L] Units: ==> umol/L Channel 17: Salinity:Bottle [PSS-78] Units: ==> PSS-78 -CALIB parameters: 2021/01/20 12:15:59 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = 2019-008-transcor.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 8 Transmissivity:Green %/metre 10 -0.2000000E-01 0.1439800E+01 -SORT parameters: 2023/03/12 19:35:38 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/12 19:36:51 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-008\Processing\IOS\2019-008-0028. che2 Secondary file: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-008\Processing\IOS\2019-008-0028. hplc 6 secondary records matched to primary records. -SORT parameters: 2023/03/12 19:37:37 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/12 19:37:52 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/19 15:05:51 - none. -REMOVECH 2023/03/19 15:06:19 The following CHANNEL(S) were removed: HPLC:B-Car -HEADER EDITS: 2023/03/19 15:06:46 Channel 31: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 55: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:35:13 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Comments from secondary file: D:\Telework\2019-008\Processing\hydro\2019-008-0028.mrgcln1s ------------------------------------------------------------ Sample_Number 198: CHL: no sample received Sample_Number 205: CHL: did not use A data; suspect that filter was askew Sample_Number 186: OXY-1: First titration did not display on screen. Sample was over-titrated. Sample_Number 198: OXY: Sample over-titrated due to a poor titration curve. Sample_Number 199: ALL: Niskin top valve was open prior to sampling. Sample_Number 202: OXY: First titration did not display on screen. Sample was over-titrated. Sample_Number 187: NUTS: Sample intended as a duplicate, however, analysis problem resulted in one sample with bad data Sample_Number 206: NUTS: Sample intended as a duplicate, however, analysis problem resulted in one sample with bad data Sample_Number 201: HPLC: >10%CV for TChl-a Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~2 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.03-10.66ug/l in 115 samples. Average %CV for this cruise was 2.80% with 0 out of 35 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is excellent. For details see worksheet “CV%” in file QF2019-008CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF2019-008_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2019-008OXY*.xlsx. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 13 - 25 days after collection. For details including a duplicate analysis, see document QF2019-008SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2019-008nuts*.xlsx. DMS samples were collected in 250mL ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100mL/min. The DMS was extracted from the water and absorbed into a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a dewar containing boiling water) onto a Chromosil 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/L, so “0” values should be interpreted as <0.1nmol/L. DMS data are limited to 2 significant figures. Unless otherwise indicated the % relative difference between duplicates is <20%. For more detail see file "2019-008 DMS report.doc" and for data and duplicate analysis see "QF2019-008_DMS*.xlsx." DMSP-D: Approximately 20-75mL of seawater was allowed to flow directly from the niskin into a magnetic filtration funnel containing a 0.7um GF/F filter. The first 3.5mL was collected in a polypropylene tube (15mL) containing 50uL of a 50% sulphuric acid solution. A new funnel and filter are used for each sample. Samples are hydrolized and run at IOS when time permits. DMPS-T: 3.5mL of seawater was collected directly from the niskin into a polypropylene tube (15mL) containing 50uL of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples are stored in the dark at 4 degrees C for a minimum of 24 hours. They are hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. DMSP data are limited to 2 significant figures. Unless otherwise indicated the % relative difference between duplicates is <20%. For details on analysis and raw data, see document "QF2019-008 dmsp*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Conductivity, Transmissivity, Fluorescence:URU:Seapoint and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2019-008_Processing_Report.doc. --------------------------------------------------------------------------------- . Comments from secondary file: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-008\Processing\IOS\2019-008-0028.hplc --------------------------------------------------------------------------- Sample_Number 201: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.83E-02 1) Salinity:T1:C1 PSS-78 10 -99 () (0.4E-03 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.182E-01 1.0348) Transmissivity:Green %/metre 10 -99 () (-0.2E-01 1.4398) $END !-1-- 2- 3- --4--- --5--- ---6---- --7-- ---8--- --9--- ---10--- --11-- --12- ----13--- -14- --15- ---16--- ---17-- --18- 19 --20-- 21 --22-- 23 ---24-- 25 --26-- 27 --28-- --29- 30 ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- ---55-- 56 !Samp Bo Bo Pressu Depth Temperat Trans Fluores PAR Salinity Oxygen Oxyge Conductiv Numb Tempe Salinity Oxygen: Oxyge Fl Nitrat Fl Silica Fl Phospha Fl Chloro Fl Phaeo- Trans Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: missi cence: :T0:C0 : n: ity: er_o ratur :Bottle Dissolv n: ag e_ ag te ag te ag phyll: ag Pigmen missi ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Primary vity URU: Dissol Disso Primary ~bin e: ed Disso ~o plus_ ~t ~i ~p Extrac ~a t: vity: ~o a chlide fuco fuco : !er mb en Seapoin ved: lved: _rec Draw lved lv Nitrit ri ca ha ted ct Extrac Green tt HP ! er ce t SBE SBE ords ed e te te te ed ted le LC !---- -- -- ------ ------ -------- ----- ------- ------ -------- ------ ----- --------- ---- ----- -------- ------- ----- -- ------ -- ------ -- ------- -- ------ -- ------ ----- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 208 23 23 5.3 5.3 17.2456 49.3 0.284 365.7 32.3306 5.77 251.9 4.204447 206 18.1 -99.0000 5.779 252.1 0 1.40 0 0.73 0 0.375 0 -99.00 0 -99.00 73.3 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 209 24 24 5.4 5.4 17.2451 49.3 0.293 369.8 32.3305 5.77 251.8 4.204387 241 -99.0 -99.0000 -99.000 -99.0 0 1.38 0 0.74 0 0.385 0 0.15 0 0.03 73.3 0 0.013 0.000 0.001 0.018 0.000 0.000 0.001 0.014 0.006 0.002 0.001 0.002 0.066 0.026 0.002 0.002 0.005 0.001 0.000 0.008 0.002 0.000 0.135 0.003 0.135 6 207 22 22 10.4 10.3 17.2503 49.4 0.297 211.2 32.3310 5.77 251.9 4.205132 241 18.1 -99.0000 5.778 252.1 0 1.40 0 0.74 0 0.382 0 0.15 0 0.03 73.4 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 206 21 21 14.9 14.8 17.2188 49.3 0.401 146.8 32.3305 5.78 252.0 4.202344 241 18.1 -99.0000 5.778 252.1 0 1.29 2 0.71 2 0.373 2 0.15 0 0.02 73.1 0 0.011 0.000 0.001 0.016 0.000 0.000 0.000 0.014 0.006 0.002 0.001 0.002 0.064 0.026 0.002 0.002 0.005 0.001 0.000 0.007 0.001 0.000 0.132 0.003 0.132 6 205 20 20 20.4 20.2 17.2090 49.3 0.405 107.6 32.3303 5.78 252.0 4.201628 241 18.1 -99.0000 5.793 252.8 6 1.87 0 0.69 0 0.360 0 0.15 2 0.02 73.1 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 204 19 19 24.9 24.7 16.0855 47.5 0.630 79.8 32.2995 6.07 264.9 4.094051 241 17.1 -99.0000 6.068 264.7 0 1.00 0 0.70 0 0.383 0 0.24 0 0.05 69.4 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 203 18 18 30.0 29.8 14.6665 46.7 0.863 71.6 32.3429 6.58 286.7 3.968594 241 15.4 -99.0000 6.608 288.1 0 0.68 0 0.73 0 0.430 0 0.29 0 0.08 66.9 0 0.046 0.000 0.003 0.044 0.000 0.000 0.000 0.043 0.016 0.004 0.004 0.003 0.174 0.023 0.000 0.003 0.010 0.003 0.003 0.025 0.005 0.000 0.269 0.005 0.269 6 202 17 17 39.6 39.3 12.6020 42.6 1.932 23.1 32.3702 7.18 313.0 3.784209 241 13.1 -99.0000 7.114 310.0 3 0.60 0 1.15 0 0.447 0 0.55 0 0.27 59.3 0 0.118 0.000 0.008 0.116 0.000 0.000 0.002 0.105 0.049 0.014 0.013 0.007 0.376 0.037 0.004 0.004 0.016 0.009 0.009 0.115 0.016 0.004 0.588 0.010 0.591 6 201 16 16 49.8 49.4 10.2352 48.9 2.241 10.1 32.3771 6.72 292.5 3.573644 241 11.1 -99.0000 6.693 291.6 0 4.32 0 3.62 0 0.681 0 0.58 0 0.27 71.4 0 0.136 0.000 0.006 0.104 0.000 0.000 0.044 0.092 0.067 0.013 0.004 0.002 0.331 0.019 0.003 0.002 0.012 0.003 0.007 0.092 0.025 0.006 0.551 0.007 0.558 36 200 15 15 59.9 59.3 9.3991 52.1 1.424 4.2 32.3710 6.57 286.0 3.499631 241 10.6 -99.0000 6.576 286.5 6 5.63 0 4.70 0 0.830 0 0.56 0 0.26 78.0 0 0.122 0.000 0.005 0.092 0.000 0.000 0.055 0.080 0.074 0.012 0.002 0.001 0.290 0.018 0.003 0.001 0.011 0.003 0.006 0.106 0.028 0.005 0.569 0.007 0.573 6 199 14 14 75.0 74.3 8.4586 56.1 0.388 1.7 32.4268 6.37 277.2 3.423148 241 9.5 -99.0000 6.353 276.7 3 8.76 3 6.65 3 0.945 3 0.15 36 0.08 85.6 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 198 13 13 100.5 99.6 7.8518 56.7 0.219 0.4 32.6527 5.78 251.5 3.392501 241 9.0 -99.0000 5.765 251.0 2 13.30 0 12.90 0 1.158 0 -99.00 9 -99.00 86.7 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 197 12 12 150.3 149.0 7.3838 57.0 0.126 0.0 33.5917 3.96 172.5 3.440552 241 8.6 -99.0000 3.950 171.9 0 25.32 0 30.44 0 1.761 0 -99.00 0 -99.00 87.2 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 196 11 11 175.2 173.6 7.2884 56.9 0.121 0.0 33.8310 3.44 149.7 3.455138 241 8.5 -99.0000 3.434 149.4 0 27.99 0 37.20 0 1.916 0 -99.00 0 -99.00 87.2 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 195 10 10 200.0 198.2 6.9737 56.9 0.120 0.0 33.8863 3.31 144.0 3.433062 241 8.3 -99.0000 3.319 144.4 6 29.49 0 41.45 0 1.983 0 -99.00 0 -99.00 87.2 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 194 9 9 249.6 247.4 6.2811 57.0 0.117 0.0 33.9123 2.62 113.8 3.375691 241 7.7 -99.0000 2.596 112.9 0 33.52 0 52.43 0 2.306 0 -99.00 0 -99.00 87.2 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 193 8 8 299.8 297.0 5.8465 57.2 0.112 0.0 33.9322 2.07 90.1 3.340992 241 7.3 -99.0000 2.069 90.0 0 36.19 0 59.99 0 2.465 0 -99.00 0 -99.00 87.5 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 192 7 7 399.8 396.1 5.1674 57.3 0.110 0.0 33.9852 1.33 58.0 3.289889 241 6.9 -99.0000 1.340 58.3 0 39.68 0 75.55 0 2.705 0 -99.00 0 -99.00 87.8 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 191 6 6 599.6 593.7 4.4297 57.6 0.109 0.0 34.1422 0.49 21.3 3.247121 241 6.3 -99.0000 0.490 21.3 0 43.71 0 100.02 0 2.977 0 -99.00 0 -99.00 88.2 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 190 5 5 800.0 791.7 3.8615 57.5 0.108 0.0 34.2828 0.25 11.0 3.217784 241 6.0 34.2817 0.268 11.6 0 44.37 0 120.56 0 3.166 0 -99.00 0 -99.00 88.1 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 189 4 4 999.9 989.1 3.4060 57.5 0.106 0.0 34.3850 0.24 10.6 3.194972 241 5.5 34.3851 0.240 10.4 0 45.41 0 135.80 0 3.230 0 -99.00 0 -99.00 88.1 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 188 3 3 1250.1 1235.8 2.8985 57.5 0.106 0.0 34.4644 0.39 17.0 3.167754 241 5.0 34.4633 0.387 16.8 0 45.03 0 151.03 0 3.208 0 -99.00 0 -99.00 88.1 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 187 2 2 1500.5 1482.5 2.4131 57.6 0.104 0.0 34.5120 0.64 28.0 3.139784 241 4.5 34.5108 0.642 27.9 0 44.77 2 164.26 2 3.126 2 -99.00 0 -99.00 88.2 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 186 1 1 2000.1 1973.7 1.9612 57.5 0.103 0.0 34.5861 1.31 57.0 3.126912 241 4.4 34.5837 1.311 57.0 26 42.98 0 176.14 0 2.985 0 -99.00 0 -99.00 88.2 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0