*2023/03/28 15:05:13.07 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/03/06 20:32:00.000 NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 51 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 33 33 2 Pressure decibar 1.3 1.3 3 Depth metres 1.3 1.3 4 Temperature:Primary 'deg C (ITS90)' 6.71 6.71 5 Salinity:T0:C0 PSS-78 28.7933 28.7933 6 Oxygen:Dissolved:SBE mL/L 7.95 7.95 7 Oxygen:Dissolved:SBE umol/kg 347.1 347.1 8 Fluorescence:URU:Wetlabs mg/m^3 10.371 10.371 9 Conductivity:Primary S/m 2.93588 2.93588 10 Depth:Nominal metres 0 0 11 Temperature:Draw 'deg C (ITS90)' -99 -99 12 Salinity:Bottle PSS-78 -99 -99 13 Flag:Salinity:Bottle ' ' 14 Nitrate_plus_Nitrite umol/L -99 -99 15 Flag:Nitrate_plus_Nitrite ' ' 16 Silicate umol/L -99 -99 17 Flag:Silicate ' ' 18 Phosphate umol/L -99 -99 19 Flag:Phosphate ' ' 20 Chlorophyll:Extracted mg/m^3 11.56 11.56 21 Flag:Chlorophyll:Extracted ' ' 22 Phaeo-Pigment:Extracted mg/m^3 3.77 3.77 23 Oxygen:Dissolved mL/L -99 -99 24 Oxygen:Dissolved umol/kg -99 -99 25 Flag:Oxygen:Dissolved ' ' 26 HPLC:Chl-c3 mg/m^3 0.25E-01 0.25E-01 27 HPLC:Chlide-a mg/m^3 1.157 1.157 28 HPLC:MgDVP mg/m^3 0.142 0.142 29 HPLC:Chl-c2 mg/m^3 1.907 1.907 30 HPLC:Chl-c1 mg/m^3 0.508 0.508 31 HPLC:Me-chlide mg/m^3 3.348 3.348 32 HPLC:Peri mg/m^3 0.51E-01 0.51E-01 33 HPLC:But-fuco mg/m^3 0.36E-01 0.36E-01 34 HPLC:Fuco mg/m^3 7.315 7.315 35 HPLC:Neo mg/m^3 0 0 36 HPLC:Pras mg/m^3 0 0 37 HPLC:Viola mg/m^3 0.14E-01 0.14E-01 38 HPLC:Hex-fuco mg/m^3 0 0 39 HPLC:Diadino mg/m^3 0.591 0.591 40 HPLC:Allo mg/m^3 0.46E-01 0.46E-01 41 HPLC:Diato mg/m^3 0.97E-01 0.97E-01 42 HPLC:Zea mg/m^3 0.9E-02 0.9E-02 43 HPLC:Lut mg/m^3 0 0 44 HPLC:Gyr-de mg/m^3 0.2E-02 0.2E-02 45 HPLC:Chl-b mg/m^3 0.48E-01 0.48E-01 46 HPLC:c2-MGDG mg/m^3 0 0 47 HPLC:DVChl-a mg/m^3 0 0 48 HPLC:Chl-a mg/m^3 8.219 8.219 49 HPLC:B-Car mg/m^3 0.171 0.171 50 HPLC:TChl-a mg/m^3 12.724 12.724 51 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 9 F R4 4 5 -99 ' ' 9 F R4 4 6 -99 ' ' 7 F R4 2 7 -99 ' ' 6 F R4 1 8 -99 ' ' 8 F R4 3 9 -99 ' ' 10 F R4 6 10 -99 ' ' 6 F R4 0 11 -99 ' ' 6 F R4 1 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 8 F R4 3 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 8 F R4 3 24 -99 ' ' 6 F ' ' 1 25 -99 ' ' 3 NQ C ' ' 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-007 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 2 EVENT NUMBER : 37 LATITUDE : 49 24.09780 N ! (deg min) LONGITUDE : 124 9.27360 W ! (deg min) WATER DEPTH : 281 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0456 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.2 2019/12/03 10:49:00 1 1 CLEAN 5.2.3 2019/12/03 10:51:33 1 1 REMOVECH 8.2 2019/12/03 10:52:06 1 1 SORT 3.6 2019/12/03 10:52:11 1 1 CHGUNITS 3.1.1 2019/12/03 10:53:28 1 1 REORDER 1.3.1 2019/12/03 10:53:37 ? ? HDREDIT2 3.1.1 2019/12/03 10:54:00 ? ? CLEAN 5.2.4 2020/07/27 16:05:05 1 1 SORT 3.6 2023/03/26 15:16:50 1 1 MERGE 3.6 2023/03/26 15:17:17 1 1 SORT 3.6 2023/03/26 15:17:53 1 1 CLEAN 5.3 2023/03/26 15:18:04 1 1 HDREDIT2 3.2 2023/03/27 16:33:26 ? ? HDREDIT2 3.2 2023/03/27 16:34:54 ? ? CLEAN 5.3 2023/03/28 15:05:13 1 1 $END $REMARKS -CLEAN functions: 2019/12/03 10:51:32 20 Change character data from " " to "0" in channels Flag:* Set header Start and End times from the data. -REMOVECH 2019/12/03 10:52:06 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2019/12/03 10:52:11 Sorted in ascending order of channel Depth:Nominal [metres] -CHANGE units: Temperature reference channel: Temperature:Draw [deg C (ITS90)] Salinity reference channel: Salinity:CTD [PSS-78] 'Oxygen:Dissolved:Bottle:Volume [mL/L]' changed from mL/L to umol/kg -HEADER EDITS: 2019/12/03 10:54:00 Applied edit header: Q:\OSD_DataProcessing\Cruise_Data\2019-007\Processing\doc\HYDRO\2019-007-bot- hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F7.2 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 8: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs Channel 9: Conductivity:Primary [S/m] Name: Conductivity:CTD ==> Conductivity:Primary Channel 10: Depth:Nominal [metres] Format: F6.0 ==> F7.1 -CLEAN functions: 2020/07/27 16:05:05 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. -SORT parameters: 2023/03/26 15:16:50 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/26 15:17:17 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2019-007\Processing\IOS\2019-007-0037.che1 Secondary file: C:\HPLC\2019-007\Processing\IOS\2019-007-0037.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/26 15:17:53 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/26 15:18:04 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 16:33:26 Applied edit header: C:\HPLC\2019-007\2019-007-bot-hdr1.txt -HEADER EDITS: 2023/03/27 16:34:54 Channel 26: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 15:05:12 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in cryovials in liquid nitro Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a standa Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~2 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Chlorophyll values ranged from 0.94 to 11.56ug/l in 14 samples. No %CV comparison quoted for this cruise because only one duplicate pair available. For details see worksheet “CV%” in file 2019-007 CHL QF*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6 weeks after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2019-007HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 145-148 days after collection. For details, see document QF2019-007SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. All analysis was performed on frozen samples. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document NUTS_QF2019-007.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876, a UV light source and detector with a 365 nm filter controlled by LVO2_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details, see document QF2019-007 OXY.xlsx. Note that samples with nominal depth 0 were surface samples with no matching CTD data. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Pena, M.A. 2014. Institue of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x +80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- For this cruise bottles were deployed as separate events from the CTD casts. The CTD data in these files were extracted from the CTD files run at the same sites, but there is some uncertainty in the depth from which the samples were taken. In the case of surface samples, the CTD data are only available from a little lower in the water column. Conductivity and Fluorescence:URU:Wetlabs data are nominal and unedited except that some records were removed in editing temperature and salinity. Calibration sampling for salinity and dissolved oxygen was done at 3 sites. There is uncertainty about the depth at which the bottles fired as the wire angle was sometimes high which would lead to bottles closing above the nominal depths. Further, there are likely errors due to incomplete flushing of Niskin bottles which also leads to bottles containing water from above the nominal depths. CTD salinity values were higher than the bottle samples by a median of 0.012psu. Some of that difference may be due to the samples coming from higher in the water column where salinity is lower. No recalibration was applied to the CTD salinity. Similarly, while Oxygen:Dissolved:SBE values appeared to be low by about 2%, samples coming from higher in the water column would mostly have higher values than found at the depth from which the CTD data come. So the error is likely <2%. No recalibration was applied to Oxygen:Dissolved:SBE. Warning: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. For details on the processing see document: 2019-007_Processing_Report.doc. --------------------------------------------------------------------------------- !-1-- --2--- --3--- ---4---- ---5---- --6--- --7-- ---8--- ----9---- --10- --11- ---12--- 13 --14-- 15 --16-- 17 ---18-- 19 --20-- 21 --22-- ---23-- --24- 25 ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- 51 !Samp Pressu Depth Temperat Salinity Oxygen Oxyge Fluores Conductiv Depth Tempe Salinity Fl Nitrat Fl Silica Fl Phospha Fl Chloro Fl Phaeo- Oxygen: Oxyge Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re ure: :T0:C0 : n: cence: ity: : ratur :Bottle ag e_ ag te ag te ag phyll: ag Pigmen Dissolv n: ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb Primary Dissol Disso URU: Primary Nomin e: ~o plus_ ~t ~i ~p Extrac ~a t: ed Disso ~o a chlide fuco fuco : !er ved: lved: Wetlabs al Draw tt Nitrit ri ca ha ted ct Extrac lved lv HP ! SBE SBE le e te te te ed ted ed LC !---- ------ ------ -------- -------- ------ ----- ------- --------- ----- ----- -------- -- ------ -- ------ -- ------- -- ------ -- ------ ------- ----- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 33 1.3 1.3 6.7100 28.7933 7.95 347.1 10.371 2.935880 0. -99.0 -99.0000 0 -99.00 0 -99.00 0 -99.000 0 11.56 0 3.77 -99.000 -99.0 0 0.025 1.157 0.142 1.907 0.508 3.348 0.051 0.036 7.315 0.000 0.000 0.014 0.000 0.591 0.046 0.097 0.009 0.000 0.002 0.048 0.000 0.000 8.219 0.171 12.724 6