*2023/03/28 10:33:49.40 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/06/07 13:34:27.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 16 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : E36D6C40 NUMBER OF CHANNELS : 56 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ------------------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 298 313 2 Bottle_Number n/a 1 16 3 Bottle:Firing_Sequence n/a 1 16 4 Pressure decibar 2.6 299.5 5 Depth metres 2.6 296.8 6 Temperature:Primary 'deg C (ITS90)' 5.1741 11.5116 7 Conductivity:Primary S/m 3.279101 3.682275 8 Transmissivity %/metre 45.3 53.6 9 Transmissivity:Green %/metre 75.4 92.8 10 Fluorescence:URU:Seapoint mg/m^3 0.122 1.876 11 PAR uE/m^2/sec 0 101.4 12 Salinity:T0:C0 PSS-78 32.3264 33.9005 13 Oxygen:Dissolved:SBE mL/L 1.97 6.95 14 Oxygen:Dissolved:SBE umol/kg 85.7 302.8 15 Number_of_bin_records n/a 234 241 16 Chlorophyll:Extracted mg/m^3 0.15 0.53 17 Flag:Chlorophyll:Extracted ' ' 18 Phaeo-Pigment:Extracted mg/m^3 0.5E-01 0.19 19 Nitrate_plus_Nitrite umol/L 3.64 37.94 20 Flag:Nitrate_plus_Nitrite ' ' 21 Silicate umol/L 2.07 69.19 22 Flag:Silicate ' ' 23 Phosphate umol/L 0.685 2.696 24 Flag:Phosphate ' ' 25 Dimethyl_Sulphide nmol/L 0 5.3 26 Flag:Dimethyl_Sulphide n/a 27 Dimethylsulfoniopropionate_Dissolved nmol/L 0.14 1.3 28 Flag:Dimethylsulfoniopropionate_Dissolved ' ' 29 Dimethylsulfoniopropionate_Total nmol/L 0.35 62 30 Flag:Dimethylsulfoniopropionate_Total ' ' 31 HPLC:Chl-c3 mg/m^3 0.34E-01 0.68E-01 32 HPLC:Chlide-a mg/m^3 0 0.2E-02 33 HPLC:MgDVP mg/m^3 0.3E-02 0.4E-02 34 HPLC:Chl-c2 mg/m^3 0.61E-01 0.93E-01 35 HPLC:Chl-c1 mg/m^3 0 0 36 HPLC:Me-chlide mg/m^3 0 0.5E-02 37 HPLC:Peri mg/m^3 0.7E-02 0.2E-01 38 HPLC:But-fuco mg/m^3 0.32E-01 0.55E-01 39 HPLC:Fuco mg/m^3 0.62E-01 0.159 40 HPLC:Neo mg/m^3 0.3E-02 0.7E-02 41 HPLC:Pras mg/m^3 0 0 42 HPLC:Viola mg/m^3 0 0.6E-02 43 HPLC:Hex-fuco mg/m^3 0.126 0.186 44 HPLC:Diadino mg/m^3 0.16E-01 0.55E-01 45 HPLC:Allo mg/m^3 0.3E-02 0.5E-02 46 HPLC:Diato mg/m^3 0.3E-02 0.7E-02 47 HPLC:Zea mg/m^3 0.4E-02 0.7E-02 48 HPLC:Lut mg/m^3 0.2E-02 0.8E-02 49 HPLC:Gyr-de mg/m^3 0.3E-02 0.4E-02 50 HPLC:Chl-b mg/m^3 0.19E-01 0.4E-01 51 HPLC:c2-MGDG mg/m^3 0.4E-02 0.11E-01 52 HPLC:DVChl-a mg/m^3 0 0 53 HPLC:Chl-a mg/m^3 0.323 0.498 54 HPLC:B-Car mg/m^3 0.3E-02 0.7E-02 55 HPLC:TChl-a mg/m^3 0.323 0.504 56 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 10 F ' ' 6 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 6 F ' ' 1 10 -99 ' ' 8 F ' ' 3 11 -99 ' ' 7 F ' ' 1 12 -99 ' ' 9 F ' ' 4 13 -99 ' ' 7 F ' ' 2 14 -99 ' ' 6 F ' ' 1 15 -99 ' ' 5 I I 0 16 -99 ' ' 7 F R4 2 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 8 F R4 3 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 3 NQ C ' ' 29 -99 ' ' 7 F R4 2 30 -99 ' ' 3 NQ C ' ' 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 8 F R4 3 56 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-006 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P16 EVENT NUMBER : 53 LATITUDE : 49 17.09000 N ! (deg min) LONGITUDE : 134 39.97000 W ! (deg min) WATER DEPTH : 3624 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS Software Version Seasave V 7.26.7.107 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.2.2 2019/10/10 09:18:42 3849 3849 CLEAN 5.2.3 2019/10/10 09:25:03 3849 3849 ADDSAMP 3.6 2019/10/10 10:44:33 3849 3849 BINAVE 4.2 2019/10/10 10:44:54 3849 16 MERGE 3.5 2019/11/13 13:43:15 16 16 CLEAN 5.2.3 2019/11/13 13:44:09 16 16 CALIB 12.0 2019/11/13 13:44:48 16 16 SORT 3.6 2019/11/13 13:45:12 16 16 REMOVECH 8.2 2019/11/13 13:45:43 16 16 CHGUNITS 3.1.1 2019/11/13 13:54:28 16 16 CHGUNITS 3.1.1 2019/11/13 14:20:34 16 16 REORDER 1.3.1 2019/11/13 14:21:46 ? ? HDREDIT2 3.1.1 2019/11/13 14:22:33 ? ? HDREDIT2 3.2 2020/12/10 11:15:16 ? ? REORDER 1.3.1 2020/12/10 11:15:54 ? ? HDREDIT2 3.2 2020/12/10 11:17:05 ? ? HDREDIT2 3.2 2020/12/10 11:19:12 ? ? CALIB 12.0.1 2021/01/20 10:25:06 16 16 REMOVECH 8.2 2021/06/03 13:28:15 16 16 MERGE 3.6 2021/06/03 13:51:59 16 16 REORDER 1.3.1 2021/06/03 14:21:38 ? ? SORT 3.6 2023/03/22 18:38:06 16 16 MERGE 3.6 2023/03/26 15:06:13 16 16 SORT 3.6 2023/03/26 15:07:44 16 16 CLEAN 5.3 2023/03/26 15:07:55 16 16 HDREDIT2 3.2 2023/03/26 15:09:14 ? ? HDREDIT2 3.2 2023/03/26 15:09:39 ? ? CLEAN 5.3 2023/03/28 10:33:49 16 16 $END $REMARKS -CLEAN functions: 2019/10/10 09:24:53 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2019/11/13 13:43:15 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Temperature:Draw, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide, Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolve, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total Primary file : Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\2019-006-0053.samavg Secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\2019-006-0053.mrgcln1s Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\2019-006-0053.mrgcln1s --------------------------------------------------------------------------- -SORT parameters: 2019/11/13 13:42:50 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2019/11/13 13:44:07 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Remove Sea-Bird comments from the header. -CALIB parameters: 2019/11/13 13:44:48 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\doc\2019-006-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Salinity:T0:C0 PSS-78 10 0.3400000E-02 0.1000000E+01 19 Salinity:T1:C1 PSS-78 10 0.6000000E-03 0.1000000E+01 20 Oxygen:Dissolved:SBE mL/L 10 0.1380000E-01 0.1035000E+01 -SORT parameters: 2019/11/13 13:45:12 Sorted in ascending order of channel Press* -REMOVECH 2019/11/13 13:45:43 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 [PSS-78] 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 [PSS-78] - No units changed -HEADER EDITS: 2019/11/13 14:22:33 Applied edit header: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\doc\HYDRO\2019-006-bot- hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F10.3 ==> F7.1 Channel 10: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 12: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 16: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 18: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 19: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 21: Silicate [umol/L] Units: ==> umol/L Channel 23: Phosphate [umol/L] Units: ==> umol/L Channel 25: Dimethyl_Sulphide [nmol/L] Units: ==> nmol/L Channel 26: Flag:Dimethyl_Sulphide [n/a] Units: ==> n/a Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F10.3 ==> F7.1 Channel 6: Conductivity:Primary [S/m] Format: F11.6 ==> F10.6 -HEADER EDITS: 2020/12/10 11:15:16 Channel 1: Bottle_Number [n/a] Format: I3 ==> I5 Channel 2: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 7: Transmissivity:Green [%/metre] Name: Transmissivity ==> Transmissivity:Green Channel 8: Transmissivity [%/metre] Name: Transmissivity2 ==> Transmissivity -HEADER EDITS: 2020/12/10 11:17:05 Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 16: Nitrate_plus_Nitrite [umol/L] Format: F6.1 ==> F7.2 -HEADER EDITS: 2020/12/10 11:19:12 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2019\2019-006-transmissivity\ 2019-006-trms-hdr1.txt -CALIB parameters: 2021/01/20 10:25:06 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = 2019-006-transcor.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 9 Transmissivity:Green %/metre 10 -0.2000000E-01 0.1439800E+01 -REMOVECH 2021/06/03 13:28:15 The following CHANNEL(S) were removed: Nitrate_plus_Nitrite [umol/L] -The following MERGE parameters were used: 2021/06/03 13:51:59 Merge Channel: Pressure Merge Scheme Used: 2: Add Secondary to Closest Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Nitrate_plus_Nitrite Primary file : Q:\OSD_DataProcessing\Cruise_Data\2019-006\old\2019-006-0053.rem Secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-006\old\2019-006-0053.nutcln 16 secondary records matched to primary records. -SORT parameters: 2023/03/22 18:38:06 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/26 15:06:13 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2019-006\Processing\IOS\2019-006-0053.che1 Secondary file: C:\HPLC\2019-006\Processing\IOS\2019-006-0053.hplc 6 secondary records matched to primary records. -SORT parameters: 2023/03/26 15:07:44 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/26 15:07:55 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/26 15:09:14 Applied edit header: C:\HPLC\2019-006\2019-006-bot-hdr1.txt -HEADER EDITS: 2023/03/26 15:09:39 Applied edit header: C:\HPLC\2019-006\2019-006-bot-hdr2.txt Channel 31: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 55: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:33:48 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\2019-006-0053.mrgcln1s --------------------------------------------------------------------------- Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\2019-006-0053.dms --------------------------------------------------------------------------- Sample_Number 310: DMS: Dewar was not fully boiling therefore my not have gotten complete desorption Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-006\Processing\hydro\2019-006-0053.dmsp --------------------------------------------------------------------------- Sample_Number 308: DMSP-T: %relative difference >20% but <49% Sample_Number 308: DMSP-D: Duplicate for this sample was lost. Result is not averaged. Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~2 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.04-26.35ug/l in 108 samples. Average %CV for this cruise was 3.56% with 2 out of 33 duplicate pairs having a CV>10% and 0 duplicate pairs having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is very good. For details see worksheet “CV%” in file QF2019-006CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~4 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2019-006HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2019-006OXY*.xlsx. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 31 - 50 days after collection. For details including a duplicate analysis, see document QF2019-006SAL*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2019-006nuts*.xlsx. DMS samples were collected in 250mL ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100mL/min. The DMS was extracted from the water and absorbed into a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a dewar containing boiling water) onto a Chromosil 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/L, so “0” values should be interpreted as <0.1nmol/L. DMS data are limited to 2 significant figures. Unless otherwise indicated the % relative difference between duplicates is <20%. For more detail see file "2019-006 DMS report.doc" and for data and duplicate analysis see "QF 2019-006 DMS*.xlsx." DMSP-D: Approximately 50-75mL of seawater was allowed to flow directly from the niskin into a filtration funnel containing a 0.7um GF/F filter. The first 3.5mL was collected in a polypropylene tube (15mL) containing 50uL of a 50% sulphuric acid solution. DMPS-T: 3.5mL of seawater was collected directly from the niskin into a polypropylene tube (15mL) containing 50uL of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples are stored in the dark at 4 degrees C for a minimum of 24 hours. They are hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. DMSP data are limited to 2 significant figures. Unless otherwise indicated the % relative difference between duplicates is <20%. For details on analysis and raw data, see document "QF2019-006 dmsp*.xlsx. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Conductivity, Transmissivity, Fluorescence:URU:Seapoint and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2019-006_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: C:\HPLC\2019-006\Processing\IOS\2019-006-0053.hplc -------------------------------------------------- Sample_Number 304: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.34E-02 1) Salinity:T1:C1 PSS-78 10 -99 () (0.6E-03 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.138E-01 1.035) Transmissivity:Green %/metre 10 -99 () (-0.2E-01 1.4398) $END !-1-- 2- 3- --4--- --5--- ---6---- ----7---- --8-- --9-- ---10-- --11-- ---12--- --13-- --14- -15- --16-- 17 --18-- --19-- 20 --21-- 22 ---23-- 24 --25-- 26 --27-- 28 --29-- 30 ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- ---55-- 56 !Samp Bo Bo Pressu Depth Temperat Conductiv Trans Trans Fluores PAR Salinity Oxygen Oxyge Numb Chloro Fl Phaeo- Nitrat Fl Silica Fl Phospha Fl Dimeth Fl Dimeth Fl Dimeth Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: ity: missi missi cence: :T0:C0 : n: er_o phyll: ag Pigmen e_ ag te ag te ag yl_ ag ylsulf ag ylsulf ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Primary Primary vity vity: URU: Dissol Disso ~bin Extrac ~a t: plus_ ~t ~i ~p Sulphi ~p ~piona ~o ~oprop ~T a chlide fuco fuco : !er mb en Green Seapoin ved: lved: _rec ted ct Extrac Nitrit ri ca ha de hi te_Dis lv ionate ot HP ! er ce t SBE SBE ords ed ted e te te te de solved ed _Total al LC !---- -- -- ------ ------ -------- --------- ----- ----- ------- ------ -------- ------ ----- ---- ------ -- ------ ------ -- ------ -- ------- -- ------ -- ------ -- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 312 15 16 2.6 2.6 11.5073 3.681099 46.2 77.2 1.080 101.4 32.3347 6.54 284.9 234 0.41 0 0.05 3.66 0 2.66 0 0.690 0 4.20 0 1.00 0 44.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 311 14 15 5.0 4.9 11.5071 3.681187 46.0 77.2 1.027 57.6 32.3348 6.53 284.6 241 0.39 0 0.06 3.67 0 2.70 0 0.696 0 5.20 0 1.30 0 62.00 0 0.037 0.001 0.003 0.062 0.000 0.000 0.017 0.033 0.081 0.004 0.000 0.005 0.128 0.055 0.005 0.007 0.004 0.006 0.003 0.019 0.004 0.000 0.379 0.007 0.380 6 310 13 14 10.2 10.2 11.5072 3.681424 46.1 77.6 1.146 30.5 32.3348 6.53 284.5 241 0.42 0 0.05 3.69 0 2.63 0 0.722 0 4.10 4 -99.00 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 309 12 13 14.8 14.7 11.5105 3.681927 46.1 77.4 1.154 20.1 32.3349 6.53 284.5 241 0.43 0 0.05 3.67 6 2.65 6 0.711 6 4.90 0 -99.00 0 -99.00 0 0.034 0.001 0.003 0.061 0.000 0.002 0.016 0.032 0.080 0.004 0.000 0.005 0.126 0.053 0.005 0.007 0.004 0.007 0.003 0.022 0.005 0.000 0.361 0.006 0.365 6 308 11 12 20.5 20.3 11.5116 3.682275 46.1 77.3 1.150 12.8 32.3350 6.53 284.5 241 0.41 0 0.06 3.64 0 2.55 0 0.691 0 4.90 6 0.93 2 51.00 36 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 307 10 11 25.9 25.7 11.5054 3.681980 46.1 77.4 1.160 8.8 32.3352 6.53 284.6 241 0.42 6 0.06 3.65 0 2.45 0 0.685 0 5.00 0 -99.00 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 306 9 10 29.9 29.6 11.4537 3.677864 45.9 76.4 1.319 6.6 32.3385 6.57 286.1 241 0.39 0 0.07 3.67 0 2.07 0 0.698 0 5.30 0 1.20 0 48.00 0 0.047 0.002 0.003 0.072 0.000 0.000 0.007 0.039 0.095 0.003 0.000 0.006 0.153 0.044 0.005 0.007 0.005 0.008 0.003 0.027 0.006 0.000 0.428 0.006 0.430 6 305 8 9 40.5 40.1 11.1051 3.649245 45.3 75.4 1.713 3.2 32.3588 6.67 290.7 241 0.53 0 0.15 4.22 0 2.22 0 0.736 0 4.10 0 -99.00 0 -99.00 0 0.068 0.002 0.004 0.093 0.000 0.005 0.010 0.055 0.159 0.007 0.000 0.004 0.174 0.034 0.004 0.006 0.005 0.007 0.004 0.040 0.007 0.000 0.498 0.007 0.504 6 313 16 8 45.0 44.7 9.2910 3.485990 46.9 78.7 1.823 2.2 32.3301 6.95 302.8 241 -99.00 0 -99.00 -99.00 0 -99.00 0 -99.000 0 -99.00 0 -99.00 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 304 7 7 50.2 49.8 8.9935 3.459754 48.3 81.6 1.876 1.5 32.3264 6.93 302.0 241 0.38 0 0.13 5.94 0 5.11 0 0.839 0 1.80 0 -99.00 0 -99.00 0 0.064 0.000 0.003 0.069 0.000 0.000 0.015 0.051 0.064 0.005 0.000 0.000 0.185 0.019 0.003 0.005 0.005 0.002 0.004 0.026 0.007 0.000 0.323 0.003 0.323 36 303 6 6 59.8 59.3 8.8102 3.445964 49.2 83.5 1.570 0.7 32.3456 6.84 297.9 241 0.36 0 0.14 5.95 0 5.39 0 0.842 0 -99.00 0 -99.00 0 -99.00 0 0.068 0.000 0.003 0.066 0.000 0.000 0.020 0.047 0.062 0.006 0.000 0.000 0.186 0.016 0.003 0.003 0.007 0.003 0.004 0.036 0.011 0.000 0.336 0.003 0.336 6 302 5 5 75.3 74.6 8.5814 3.427327 50.5 85.6 1.229 0.2 32.3529 6.68 291.1 241 0.26 0 0.19 6.44 0 5.88 0 0.894 0 0.59 0 -99.00 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 301 4 4 100.5 99.7 7.7762 3.373091 52.4 90.3 0.466 0.0 32.5109 6.24 271.6 241 0.15 0 0.13 12.18 0 11.83 0 1.169 0 0.32 0 -99.00 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 300 3 3 151.1 149.8 6.7018 3.387655 53.6 92.8 0.139 0.0 33.6728 3.70 161.0 241 -99.00 0 -99.00 27.86 0 38.84 0 2.014 0 0.00 0 0.16 0 0.94 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 299 2 2 200.6 198.8 6.1774 3.358801 53.5 92.6 0.127 0.0 33.8462 3.27 142.1 241 -99.00 0 -99.00 30.97 0 49.36 0 2.188 0 0.00 0 0.14 0 0.35 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 298 1 1 299.5 296.8 5.1741 3.279101 53.6 92.8 0.122 0.0 33.9005 1.97 85.7 241 -99.00 0 -99.00 37.94 0 69.19 0 2.696 0 0.00 0 -99.00 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0