*2023/03/28 10:19:56.36 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/05/29 14:12:51.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 17 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 18F6A789 NUMBER OF CHANNELS : 57 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 314 330 2 Bottle_Number n/a 1 17 3 Bottle:Firing_Sequence n/a 1 17 4 Pressure decibar 5 771.6 5 Depth metres 5 763.6 6 Temperature:Primary 'deg C (ITS90)' 4.0488 11.1241 7 Transmissivity %/metre 28.6 53.6 8 Fluorescence:URU:Seapoint mg/m^3 0.113 4.393 9 PAR uE/m^2/sec 0 77.2 10 Salinity:T0:C0 PSS-78 32.3152 34.2931 11 Oxygen:Dissolved:SBE mL/L 0.24 6.85 12 Oxygen:Dissolved:SBE umol/kg 10.4 298.6 13 Conductivity:Primary S/m 3.234413 3.645147 14 Number_of_bin_records n/a 241 241 15 Temperature:Draw 'deg C (ITS90)' 6.3 12.1 16 Salinity:Bottle PSS-78 32.32 34.28 17 Flag:Salinity:Bottle n/a 18 Oxygen:Dissolved mL/L 0.231 6.869 19 Flag:Oxygen:Dissolved ' ' 20 Nitrate_plus_Nitrite umol/L 0.3 44.3 21 Flag:Nitrate_plus_Nitrite ' ' 22 Silicate umol/L 2.38 117.19 23 Flag:Silicate ' ' 24 Phosphate umol/L 0.372 3.282 25 Flag:Phosphate ' ' 26 Transmissivity:Green %/metre 41.4 92.3 27 Ammonium umol/L 0.13 0.31 28 Flag:Ammonium ' ' 29 Chlorophyll:Extracted mg/m^3 0.19 3.05 30 Flag:Chlorophyll:Extracted ' ' 31 Phaeo-Pigment:Extracted mg/m^3 0.28 1.28 32 HPLC:Chl-c3 mg/m^3 0.188 0.188 33 HPLC:Chlide-a mg/m^3 0.11 0.11 34 HPLC:MgDVP mg/m^3 0.5E-01 0.5E-01 35 HPLC:Chl-c2 mg/m^3 0.385 0.385 36 HPLC:Chl-c1 mg/m^3 0.19E-01 0.19E-01 37 HPLC:Me-chlide mg/m^3 0.311 0.311 38 HPLC:Peri mg/m^3 0.28E-01 0.28E-01 39 HPLC:But-fuco mg/m^3 0.42E-01 0.42E-01 40 HPLC:Fuco mg/m^3 1.137 1.137 41 HPLC:Neo mg/m^3 0.8E-01 0.8E-01 42 HPLC:Pras mg/m^3 0.111 0.111 43 HPLC:Viola mg/m^3 0.65E-01 0.65E-01 44 HPLC:Hex-fuco mg/m^3 0.248 0.248 45 HPLC:Diadino mg/m^3 0.185 0.185 46 HPLC:Allo mg/m^3 0.104 0.104 47 HPLC:Diato mg/m^3 0.8E-02 0.8E-02 48 HPLC:Zea mg/m^3 0.24E-01 0.24E-01 49 HPLC:Lut mg/m^3 0.1E-01 0.1E-01 50 HPLC:Gyr-de mg/m^3 0.9E-02 0.9E-02 51 HPLC:Chl-b mg/m^3 0.44 0.44 52 HPLC:c2-MGDG mg/m^3 0.35E-01 0.35E-01 53 HPLC:DVChl-a mg/m^3 0 0 54 HPLC:Chl-a mg/m^3 2.447 2.447 55 HPLC:B-Car mg/m^3 0.61E-01 0.61E-01 56 HPLC:TChl-a mg/m^3 2.868 2.868 57 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 6 F ' ' 1 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 7 F ' ' 1 10 -99 ' ' 9 F ' ' 4 11 -99 ' ' 7 F ' ' 2 12 -99 ' ' 6 F ' ' 1 13 -99 ' ' 10 F ' ' 6 14 -99 ' ' 5 I I 0 15 -99 ' ' 6 F R4 1 16 -99 ' ' 9 F R4 4 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 8 F R4 3 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 8 F R4 3 25 -99 ' ' 3 NQ C ' ' 26 -99 ' ' 6 F ' ' 1 27 -99 ' ' 7 F R4 2 28 -99 ' ' 3 NQ C ' ' 29 -99 ' ' 7 F R4 2 30 -99 ' ' 3 NQ C ' ' 31 -99 ' ' 7 F R4 2 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 8 F R4 3 56 -99 ' ' 8 F R4 3 57 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-005 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : LD08 EVENT NUMBER : 124 LATITUDE : 48 39.21000 N ! (deg min) LONGITUDE : 126 23.50000 W ! (deg min) WATER DEPTH : 773.5 ALTIMETER (M) : 10.28 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 $REMARKS Software Version Seasave V 7.26.7.107 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.2.2 2019/09/03 09:26:25 4097 4097 CLEAN 5.2.3 2019/09/03 09:26:52 4097 4097 ADDSAMP 3.6 2019/09/03 15:23:39 4097 4097 BINAVE 4.2 2019/09/03 15:24:07 4097 17 MERGE 3.5 2019/10/31 15:22:35 17 17 CLEAN 5.2.3 2019/10/31 15:22:40 17 17 MERGEHDR 1.3 2019/10/31 15:23:23 17 17 CALIB 12.0 2019/10/31 15:24:19 17 17 SORT 3.6 2019/10/31 15:24:26 17 17 REMOVECH 8.2 2019/10/31 15:24:55 17 17 CHGUNITS 3.1.1 2019/10/31 15:25:58 17 17 CHGUNITS 3.1.1 2019/10/31 15:26:16 17 17 REORDER 1.3.1 2019/10/31 15:27:11 ? ? HDREDIT2 3.1.1 2019/10/31 15:27:19 ? ? HDREDIT2 3.2 2020/12/10 09:48:33 ? ? REORDER 1.3.1 2020/12/10 09:50:14 ? ? HDREDIT2 3.2 2020/12/10 10:32:21 ? ? HDREDIT2 3.2 2020/12/10 10:39:50 ? ? CALIB 12.0.1 2021/01/20 09:19:49 17 17 SORT 3.6 2023/03/19 14:31:59 17 17 REORDER 1.3.1 2023/03/19 14:41:49 ? ? MERGE 3.6 2023/03/19 14:45:58 17 17 SORT 3.6 2023/03/19 14:47:48 17 17 CLEAN 5.3 2023/03/19 14:48:08 17 17 HDREDIT2 3.2 2023/03/19 14:48:56 ? ? HDREDIT2 3.2 2023/03/19 14:49:56 ? ? CLEAN 5.3 2023/03/28 10:19:56 17 17 $END $REMARKS -CLEAN functions: 2019/09/03 09:26:40 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2019/10/31 15:22:35 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Temperature:Draw, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide, Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolve, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total Primary file : Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0124.samavg Secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0124.mrgcln1s Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0124.mrgcln1s --------------------------------------------------------------------------- -CLEAN functions: 2019/10/31 15:22:15 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2019/10/31 15:22:23 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2019/10/31 15:22:38 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Remove Sea-Bird comments from the header. -CALIB parameters: 2019/10/31 15:24:19 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\doc\2019-005-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Salinity:T0:C0 PSS-78 10 0.2300000E-02 0.1000000E+01 20 Oxygen:Dissolved:SBE mL/L 10 0.2260000E-01 0.1030000E+01 -SORT parameters: 2019/10/31 15:24:26 Sorted in ascending order of channel Press* -REMOVECH 2019/10/31 15:24:55 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -HEADER EDITS: 2019/10/31 15:27:19 Applied edit header: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\doc\HYDRO\2019-005-bot- hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F10.3 ==> F7.1 Channel 10: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 12: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 16: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Format: F7.2 ==> F6.1 Channel 17: Salinity:Bottle [PSS-78] Units: ==> PSS-78 Channel 18: Flag:Salinity:Bottle [n/a] Units: ==> n/a Channel 19: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 21: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 22: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 24: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 26: Silicate [umol/L] Units: ==> umol/L Channel 28: Phosphate [umol/L] Units: ==> umol/L Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F10.3 ==> F7.1 Channel 6: Conductivity:Primary [S/m] Format: F11.6 ==> F10.6 -HEADER EDITS: 2020/12/10 09:48:33 Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 1: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 7: Transmissivity:Green [%/metre] Name: Transmissivity ==> Transmissivity:Green Channel 8: Transmissivity [%/metre] Name: Transmissivity2 ==> Transmissivity -HEADER EDITS: 2020/12/10 10:32:21 Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 17: Salinity:Bottle [PSS-78] Format: F7.2 ==> F9.4 Channel 21: Nitrate_plus_Nitrite [umol/L] Format: F6.1 ==> F7.2 -HEADER EDITS: 2020/12/10 10:39:50 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2019\2019-005-transmissivity\ 2019-005-trms-hdr1.txt -CALIB parameters: 2021/01/20 09:19:49 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = 2019-005-transcor.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 9 Transmissivity:Green %/metre 10 -0.2000000E-01 0.1439800E+01 -SORT parameters: 2023/03/19 14:31:59 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/19 14:45:58 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2019-005\Processing\IOS\2019-005-0124.che2 Secondary file: C:\HPLC\2019-005\Processing\IOS\2019-005-0124.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/19 14:47:48 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/19 14:48:07 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/19 14:48:56 Applied edit header: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-005\2019-005-bot-hdr1.txt -HEADER EDITS: 2023/03/19 14:49:56 Applied edit header: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-005\2019-005-bot-hdr2.txt Channel 32: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 55: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 56: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:19:54 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0124.mrgcln1s --------------------------------------------------------------------------- Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0124.sal --------------------------------------------------------------------------- Sample_Number 330: SAL: Flushed x4 and then x2 due to bubble forming near top coil Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0124.oxy --------------------------------------------------------------------------- Sample_Number 320: OXY-1: Sample over-titrated due to late endpoint. Sample_Number 322: OXY: Sample over-titrated due to late endpoint. Sample_Number 329: OXY: Sample over-titrated due to late endpoint. Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~3 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.09-26.73ug/l in 220 samples. Average %CV for this cruise was 4.50% with 3 out of 54 duplicate pairs having a CV>10% and 1 duplicate pair having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is good. For details see worksheet “CV%” in file QF2019-005CHL*.xls. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2.5 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2019-005HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2019-005OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 52 - 63 days after collection. For details including a duplicate analysis, see document QF2019-005SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2019-005nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. Standards are made in LNSW collected at 250m at station LB14. For full details including a precision study see file 2019-005NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- The cast at station CS02 was recorded in 3 different files, named 2019-005-0061, 2019-005-0062 and 2019-005-0063. Bottles were fired during the 1st and 3rd of those files and the CTD data were joined into a single file which was named 2019-005-0061. Conductivity, Transmissivity, Fluorescence:URU:Seapoint and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2019-005_Processing_Report.doc. --------------------------------------------------------------------------------- *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.23E-02 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.226E-01 1.03) Transmissivity:Green %/metre 10 -99 () (-0.2E-01 1.4398) $END !-1-- 2- 3- --4--- --5--- ---6---- --7-- ---8--- --9--- ---10--- --11-- --12- ----13--- -14- --15- ---16--- 17 ---18-- 19 --20-- 21 --22-- 23 ---24-- 25 --26- --27-- 28 --29-- 30 --31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- ---55-- ---56-- 57 !Samp Bo Bo Pressu Depth Temperat Trans Fluores PAR Salinity Oxygen Oxyge Conductiv Numb Tempe Salinity Fl Oxygen: Fl Nitrat Fl Silica Fl Phospha Fl Trans Ammoni Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: missi cence: :T0:C0 : n: ity: er_o ratur :Bottle ag Dissolv ag e_ ag te ag te ag missi um ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Primary vity URU: Dissol Disso Primary ~bin e: ~o ed ~o plus_ ~t ~i ~p vity: ~o Extrac ~a t: a chlide fuco fuco : !er mb en Seapoin ved: lved: _rec Draw tt lv Nitrit ri ca ha Green ni ted ct Extrac HP ! er ce t SBE SBE ords le ed e te te te um ed ted LC !---- -- -- ------ ------ -------- ----- ------- ------ -------- ------ ----- --------- ---- ----- -------- -- ------- -- ------ -- ------ -- ------- -- ----- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 330 17 17 5.0 5.0 11.1241 28.6 4.102 77.2 32.3155 6.85 298.6 3.645147 241 12.1 32.3200 2 6.869 0 0.30 0 2.40 0 0.388 0 41.4 0.13 6 2.85 0 1.28 0.188 0.110 0.050 0.385 0.019 0.311 0.028 0.042 1.137 0.080 0.111 0.065 0.248 0.185 0.104 0.008 0.024 0.010 0.009 0.440 0.035 0.000 2.447 0.061 2.868 6 329 16 16 10.3 10.2 11.1077 28.6 4.393 25.1 32.3152 6.84 297.9 3.643871 241 11.9 -99.0000 0 6.848 2 0.50 0 2.38 0 0.372 0 41.6 0.14 6 3.05 0 1.08 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 328 15 15 20.7 20.5 10.3097 40.4 1.857 3.9 32.3441 6.55 285.3 3.576303 241 11.3 -99.0000 0 6.578 6 1.20 0 2.72 0 0.486 0 65.8 0.31 6 1.31 0 0.85 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 327 14 14 30.0 29.8 10.0195 44.5 1.261 1.2 32.3647 6.44 280.4 3.553058 241 10.9 -99.0000 0 6.454 0 2.30 0 3.44 0 0.561 0 73.6 -99.00 0 0.90 6 0.67 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 326 13 13 50.3 49.9 8.2068 51.7 0.396 0.2 32.6518 5.72 249.2 3.421911 241 9.3 -99.0000 0 5.635 0 12.30 0 13.15 0 1.147 0 88.1 -99.00 0 0.19 0 0.28 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 325 12 12 75.5 74.8 8.0614 52.2 0.169 0.0 33.2984 4.11 178.9 3.471010 241 9.2 -99.0000 0 4.096 0 21.90 6 23.98 6 1.628 6 89.2 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 324 11 11 100.4 99.6 7.8659 52.2 0.150 0.0 33.6105 3.39 147.6 3.483813 241 9.0 -99.0000 0 3.396 0 26.10 0 30.82 0 1.864 0 89.2 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 323 10 10 125.8 124.7 7.7073 52.1 0.134 0.0 33.8660 2.81 122.4 3.494440 241 8.9 -99.0000 0 2.808 0 29.40 0 38.44 0 2.024 0 89.1 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 322 9 9 150.0 148.7 7.4551 52.9 0.134 0.0 33.9154 2.87 124.9 3.477347 241 8.6 -99.0000 0 2.867 2 29.90 0 41.19 0 2.056 0 90.8 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 321 8 8 175.3 173.8 7.3011 53.1 0.130 0.0 33.9399 2.57 111.6 3.466850 241 8.5 -99.0000 0 2.571 0 31.30 0 44.37 0 2.203 0 91.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 320 7 7 200.6 198.8 6.9135 53.3 0.127 0.0 33.9428 2.43 105.7 3.433389 241 8.3 -99.0000 0 2.426 26 32.60 0 48.78 0 2.300 0 91.7 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 319 6 6 250.7 248.4 6.3647 53.6 0.123 0.0 33.9627 2.01 87.2 3.388254 241 7.8 -99.0000 0 1.997 0 35.30 0 56.89 0 2.471 0 92.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 318 5 5 301.2 298.5 5.9957 53.6 0.122 0.0 33.9890 1.60 69.7 3.359913 241 7.6 -99.0000 0 1.607 0 37.40 0 63.77 0 2.626 0 92.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 317 4 4 400.9 397.2 5.3649 53.6 0.122 0.0 34.0341 1.12 48.7 3.312218 241 7.2 -99.0000 0 1.117 0 40.10 0 76.72 0 2.881 0 92.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 316 3 3 500.6 495.7 4.9706 53.5 0.116 0.0 34.0944 0.74 32.2 3.286938 241 6.9 34.0900 0 0.743 0 41.80 0 87.39 0 3.004 0 92.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 315 2 2 751.2 743.5 4.1269 52.8 0.113 0.0 34.2744 0.25 11.0 3.238823 241 6.4 34.2800 0 0.246 0 44.00 6 114.36 6 3.282 6 90.7 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 314 1 1 771.6 763.6 4.0488 52.6 0.113 0.0 34.2931 0.24 10.4 3.234413 241 6.3 -99.0000 0 0.231 0 44.30 0 117.19 0 3.249 0 90.4 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0