*2023/03/28 10:19:55.27 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2019/05/25 11:23:26.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 12 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 189B3F3B NUMBER OF CHANNELS : 55 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 77 88 2 Bottle_Number n/a 1 12 3 Bottle:Firing_Sequence n/a 1 12 4 Pressure decibar 5.1 186.8 5 Depth metres 5.1 185.1 6 Temperature:Primary 'deg C (ITS90)' 6.6264 10.3251 7 Transmissivity %/metre 48.1 53.9 8 Fluorescence:URU:Seapoint mg/m^3 0.143 0.516 9 PAR uE/m^2/sec 0 0.6 10 Salinity:T0:C0 PSS-78 31.7399 33.8893 11 Oxygen:Dissolved:SBE mL/L 1.87 6.41 12 Oxygen:Dissolved:SBE umol/kg 81.5 279.4 13 Conductivity:Primary S/m 3.39747 3.517216 14 Number_of_bin_records n/a 241 241 15 Temperature:Draw 'deg C (ITS90)' 7.7 10.7 16 Oxygen:Dissolved mL/L 1.862 6.438 17 Flag:Oxygen:Dissolved ' ' 18 Nitrate_plus_Nitrite umol/L 4.6 34 19 Flag:Nitrate_plus_Nitrite ' ' 20 Silicate umol/L 10.57 59.05 21 Flag:Silicate ' ' 22 Phosphate umol/L 0.777 2.507 23 Flag:Phosphate ' ' 24 Transmissivity:Green %/metre 75.6 86.7 25 Ammonium umol/L 1.59 1.6 26 Flag:Ammonium ' ' 27 Chlorophyll:Extracted mg/m^3 0.13 0.99 28 Flag:Chlorophyll:Extracted ' ' 29 Phaeo-Pigment:Extracted mg/m^3 0.14 0.3 30 HPLC:Chl-c3 mg/m^3 0.1E-01 0.1E-01 31 HPLC:Chlide-a mg/m^3 0 0 32 HPLC:MgDVP mg/m^3 0.2E-02 0.2E-02 33 HPLC:Chl-c2 mg/m^3 0.43E-01 0.43E-01 34 HPLC:Chl-c1 mg/m^3 0.6E-02 0.6E-02 35 HPLC:Me-chlide mg/m^3 0 0 36 HPLC:Peri mg/m^3 0.14E-01 0.14E-01 37 HPLC:But-fuco mg/m^3 0.2E-02 0.2E-02 38 HPLC:Fuco mg/m^3 0.113 0.113 39 HPLC:Neo mg/m^3 0 0 40 HPLC:Pras mg/m^3 0 0 41 HPLC:Viola mg/m^3 0 0 42 HPLC:Hex-fuco mg/m^3 0.13E-01 0.13E-01 43 HPLC:Diadino mg/m^3 0.18E-01 0.18E-01 44 HPLC:Allo mg/m^3 0.29E-01 0.29E-01 45 HPLC:Diato mg/m^3 0 0 46 HPLC:Zea mg/m^3 0.1E-02 0.1E-02 47 HPLC:Lut mg/m^3 0 0 48 HPLC:Gyr-de mg/m^3 0 0 49 HPLC:Chl-b mg/m^3 0.3E-02 0.3E-02 50 HPLC:c2-MGDG mg/m^3 0.2E-02 0.2E-02 51 HPLC:DVChl-a mg/m^3 0 0 52 HPLC:Chl-a mg/m^3 0.298 0.298 53 HPLC:B-Car mg/m^3 0.3E-02 0.3E-02 54 HPLC:TChl-a mg/m^3 0.298 0.298 55 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 7 F ' ' 1 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 6 F ' ' 1 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 7 F ' ' 1 10 -99 ' ' 9 F ' ' 4 11 -99 ' ' 7 F ' ' 2 12 -99 ' ' 6 F ' ' 1 13 -99 ' ' 10 F ' ' 6 14 -99 ' ' 5 I I 0 15 -99 ' ' 6 F R4 1 16 -99 ' ' 8 F R4 3 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 8 F R4 3 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 6 F ' ' 1 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 3 NQ C ' ' 29 -99 ' ' 7 F R4 2 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2019-005 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : CS09 EVENT NUMBER : 36 LATITUDE : 51 12.53000 N ! (deg min) LONGITUDE : 128 27.83000 W ! (deg min) WATER DEPTH : 194.5 ALTIMETER (M) : 9.07 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 $REMARKS Software Version Seasave V 7.26.7.107 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 4.2.2 2019/09/03 09:26:15 2892 2892 CLEAN 5.2.3 2019/09/03 09:26:43 2892 2892 CTDEDIT 5.2.3 2019/09/03 10:13:54 2892 2892 ADDSAMP 3.6 2019/09/03 15:23:31 2892 2892 BINAVE 4.2 2019/09/03 15:24:02 2892 12 MERGE 3.5 2019/10/31 15:22:34 12 12 CLEAN 5.2.3 2019/10/31 15:22:39 12 12 MERGEHDR 1.3 2019/10/31 15:23:22 12 12 CALIB 12.0 2019/10/31 15:24:18 12 12 SORT 3.6 2019/10/31 15:24:25 12 12 REMOVECH 8.2 2019/10/31 15:24:54 12 12 CHGUNITS 3.1.1 2019/10/31 15:25:57 12 12 CHGUNITS 3.1.1 2019/10/31 15:26:15 12 12 REORDER 1.3.1 2019/10/31 15:27:10 ? ? HDREDIT2 3.1.1 2019/10/31 15:27:18 ? ? HDREDIT2 3.2 2020/12/10 09:48:32 ? ? REORDER 1.3.1 2020/12/10 09:50:12 ? ? HDREDIT2 3.2 2020/12/10 10:32:19 ? ? HDREDIT2 3.2 2020/12/10 10:39:48 ? ? CALIB 12.0.1 2021/01/20 09:19:48 12 12 SORT 3.6 2023/03/19 14:31:58 12 12 REORDER 1.3.1 2023/03/19 14:41:48 ? ? MERGE 3.6 2023/03/19 14:45:57 12 12 SORT 3.6 2023/03/19 14:47:48 12 12 CLEAN 5.3 2023/03/19 14:48:07 12 12 HDREDIT2 3.2 2023/03/19 14:48:56 ? ? HDREDIT2 3.2 2023/03/19 14:49:56 ? ? CLEAN 5.3 2023/03/28 10:19:55 12 12 $END $REMARKS -CLEAN functions: 2019/09/03 09:26:40 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -CTDEDIT v5.2.3 2019/09/03 10:13:54 5 changes in channel Salinity:T0:C0 -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2019/10/31 15:22:34 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Temperature:Draw, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide, Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolve, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total Primary file : Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0036.samavg Secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0036.mrgcln1s Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0036.mrgcln1s --------------------------------------------------------------------------- -CLEAN functions: 2019/10/31 15:22:15 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2019/10/31 15:22:22 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2019/10/31 15:22:38 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Remove Sea-Bird comments from the header. -CALIB parameters: 2019/10/31 15:24:18 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\doc\2019-005-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Salinity:T0:C0 PSS-78 10 0.2300000E-02 0.1000000E+01 20 Oxygen:Dissolved:SBE mL/L 10 0.2260000E-01 0.1030000E+01 -SORT parameters: 2019/10/31 15:24:25 Sorted in ascending order of channel Press* -REMOVECH 2019/10/31 15:24:54 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -HEADER EDITS: 2019/10/31 15:27:18 Applied edit header: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\doc\HYDRO\2019-005-bot- hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F10.3 ==> F7.1 Channel 10: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 12: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 16: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Format: F7.2 ==> F6.1 Channel 17: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 19: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 20: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 22: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 24: Silicate [umol/L] Units: ==> umol/L Channel 26: Phosphate [umol/L] Units: ==> umol/L Channel 4: Depth [metres] Name: Depth:Salt_Water ==> Depth Format: F10.3 ==> F7.1 Channel 6: Conductivity:Primary [S/m] Format: F11.6 ==> F10.6 -HEADER EDITS: 2020/12/10 09:48:32 Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 1: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 7: Transmissivity:Green [%/metre] Name: Transmissivity ==> Transmissivity:Green Channel 8: Transmissivity [%/metre] Name: Transmissivity2 ==> Transmissivity -HEADER EDITS: 2020/12/10 10:32:19 Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 19: Nitrate_plus_Nitrite [umol/L] Format: F6.1 ==> F7.2 -HEADER EDITS: 2020/12/10 10:39:48 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2019\2019-005-transmissivity\ 2019-005-trms-hdr1.txt -CALIB parameters: 2021/01/20 09:19:48 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = 2019-005-transcor.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 9 Transmissivity:Green %/metre 10 -0.2000000E-01 0.1439800E+01 -SORT parameters: 2023/03/19 14:31:58 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2023/03/19 14:45:57 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Gyr-de, HPLC:Chl-b, HPLC:c2-MGDG, HPLC:DVChl-a, HPLC:Phe, HPLC:Chl-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : C:\HPLC\2019-005\Processing\IOS\2019-005-0036.che2 Secondary file: C:\HPLC\2019-005\Processing\IOS\2019-005-0036.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/19 14:47:48 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/19 14:48:07 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/19 14:48:56 Applied edit header: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-005\2019-005-bot-hdr1.txt -HEADER EDITS: 2023/03/19 14:49:56 Applied edit header: C:\Users\jared\OneDrive\Documents\DFO\HPLC\2019-005\2019-005-bot-hdr2.txt Channel 30: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:c2-MGDG [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2023/03/28 10:19:54 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Remarks from CTDEDIT: CTDEDIT was used to clean a few points in channel Salinity:T0:C0 around 175db. Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0036.mrgcln1s --------------------------------------------------------------------------- Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\2019-005\Processing\hydro\2019-005-0036.oxy --------------------------------------------------------------------------- Sample_Number 78: OXY-2: Sample over-titrated due to a bad endpoint. Sample_Number 79: OXY: Sample over-titrated due to bad endpoint. Sample_Number 84: OXY: Sample over-titrated due to late endpoint. Sample_Number 87: ALL: Niskin had a leaky top cap prior to sampling. Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~3 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.09-26.73ug/l in 220 samples. Average %CV for this cruise was 4.50% with 3 out of 54 duplicate pairs having a CV>10% and 1 duplicate pair having a CV>30%. Our average dataset %CV is 3.80% for 2013 - 2018 so the overall quality of this dataset is good. For details see worksheet “CV%” in file QF2019-005CHL*.xls. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2.5 months after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2019-005HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2019-005OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 52 - 63 days after collection. For details including a duplicate analysis, see document QF2019-005SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2019-005nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. Standards are made in LNSW collected at 250m at station LB14. For full details including a precision study see file 2019-005NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, B. A., and Peterson, B.J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- The cast at station CS02 was recorded in 3 different files, named 2019-005-0061, 2019-005-0062 and 2019-005-0063. Bottles were fired during the 1st and 3rd of those files and the CTD data were joined into a single file which was named 2019-005-0061. Conductivity, Transmissivity, Fluorescence:URU:Seapoint and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 2 WetLabs CStar transmissometers in use during this cruise: Channel Transmissometer refers to sensor #1185DR (650nm - red) Channel Transmissometer:Green refers to sensor #1883DG (530nm - green) For comparison with other Institute of Ocean Sciences cruises, note that the transmissometer wavelength is 650nm unless otherwise stated. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2019-005_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: C:\HPLC\2019-005\Processing\IOS\2019-005-0036.hplc -------------------------------------------------- Sample_Number 87: HPLC: >10%CV for TChl-a, value low compared to Turner and Seapoint *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- --------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.23E-02 1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.226E-01 1.03) Transmissivity:Green %/metre 10 -99 () (-0.2E-01 1.4398) $END !-1-- 2- 3- --4--- --5--- ---6---- --7-- ---8--- --9--- ---10--- --11-- --12- ----13--- -14- --15- ---16-- 17 --18-- 19 --20-- 21 ---22-- 23 --24- --25-- 26 --27-- 28 --29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- 55 !Samp Bo Bo Pressu Depth Temperat Trans Fluores PAR Salinity Oxygen Oxyge Conductiv Numb Tempe Oxygen: Fl Nitrat Fl Silica Fl Phospha Fl Trans Ammoni Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: missi cence: :T0:C0 : n: ity: er_o ratur Dissolv ag e_ ag te ag te ag missi um ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Car TChl-a ag !Numb ~u ~u Primary vity URU: Dissol Disso Primary ~bin e: ed ~o plus_ ~t ~i ~p vity: ~o Extrac ~a t: a chlide fuco fuco : !er mb en Seapoin ved: lved: _rec Draw lv Nitrit ri ca ha Green ni ted ct Extrac HP ! er ce t SBE SBE ords ed e te te te um ed ted LC !---- -- -- ------ ------ -------- ----- ------- ------ -------- ------ ----- --------- ---- ----- ------- -- ------ -- ------ -- ------- -- ----- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 87 11 12 5.1 5.1 10.3251 51.7 0.438 0.6 31.7407 6.41 279.4 3.516883 241 10.7 6.429 3 4.60 3 10.62 3 0.794 3 82.5 1.59 36 0.99 0 0.14 0.010 0.000 0.002 0.043 0.006 0.000 0.014 0.002 0.113 0.000 0.000 0.000 0.013 0.018 0.029 0.000 0.001 0.000 0.000 0.003 0.002 0.000 0.298 0.003 0.298 36 86 10 11 10.8 10.7 10.3244 51.7 0.446 0.1 31.7401 6.41 279.4 3.516996 241 10.7 6.432 0 4.60 0 10.61 0 0.782 0 82.6 1.60 6 0.54 6 0.17 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 85 9 10 20.1 19.9 10.3227 51.8 0.450 0.0 31.7399 6.41 279.3 3.517216 241 10.7 6.438 0 4.60 0 10.57 0 0.777 0 82.6 1.60 6 0.55 0 0.21 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 88 12 9 28.1 27.9 10.2016 51.3 0.479 0.0 31.8082 6.34 276.5 3.513808 241 -99.0 -99.000 0 -99.00 0 -99.00 0 -99.000 0 81.6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 84 8 8 29.9 29.6 10.1654 50.9 0.516 0.0 31.8280 6.33 275.9 3.512690 241 10.5 6.333 2 5.50 6 11.46 6 0.834 6 80.9 -99.00 0 0.55 0 0.26 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 83 7 7 49.8 49.4 8.4959 53.9 0.216 0.0 32.2286 5.84 254.2 3.407026 241 9.2 5.897 0 10.50 0 15.04 0 1.104 0 86.7 -99.00 0 0.13 0 0.30 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 82 6 6 75.5 74.8 8.0088 53.9 0.171 0.0 32.5648 4.84 210.6 3.397470 241 8.8 4.859 0 17.80 0 25.62 0 1.509 0 86.5 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 81 5 5 100.6 99.7 7.7117 53.0 0.148 0.0 33.1885 3.33 145.0 3.430743 241 8.6 3.396 0 24.90 0 37.99 0 1.975 0 84.8 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 80 4 4 124.0 122.9 7.3131 51.7 0.148 0.0 33.5194 2.67 116.2 3.426979 241 8.3 2.683 0 28.80 0 46.52 0 2.147 0 82.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 79 3 3 151.1 149.7 6.8834 50.9 0.147 0.0 33.7789 2.11 91.9 3.413581 241 7.9 2.118 2 32.40 0 54.19 0 2.377 0 80.9 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 78 2 2 175.5 173.9 6.6863 49.1 0.143 0.0 33.8756 1.89 82.2 3.405754 241 7.8 1.893 6 33.80 0 58.12 0 2.477 0 77.3 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 77 1 1 186.8 185.1 6.6264 48.1 0.145 0.0 33.8893 1.87 81.5 3.402198 241 7.7 1.862 0 34.00 0 59.05 0 2.507 0 75.6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0