*2025/02/13 09:48:52.44 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2018/03/04 17:53:00.000 NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 42 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 11 11 2 Pressure decibar 2 2 3 Depth metres 2 2 4 Temperature:Primary 'deg C (ITS90)' 6.94 6.94 5 Salinity:CTD PSS-78 27.8322 27.8322 6 Oxygen:Dissolved mL/L 6.99 6.99 7 Oxygen:Dissolved umol/kg 305.4 305.4 8 Fluorescence:URU:Wetlabs mg/m^3 1.148 1.148 9 pH:SBE:Nominal n/a 8.837 8.837 10 Conductivity:Primary S/m 2.864251 2.864251 11 Depth:Nominal metres 3 3 12 Chlorophyll:Extracted mg/m^3 2 2 13 Flag:Chlorophyll:Extracted n/a 14 Phaeo-Pigment:Extracted mg/m^3 5 5 15 HPLC:Chl-c3 mg/m^3 0.166 0.166 16 HPLC:Chlide-a mg/m^3 0.11E-01 0.11E-01 17 HPLC:MgDVP mg/m^3 0.32E-01 0.32E-01 18 HPLC:Chl-c2 mg/m^3 0.325 0.325 19 HPLC:Chl-c1 mg/m^3 0.113 0.113 20 HPLC:Me-chlide mg/m^3 0.84E-01 0.84E-01 21 HPLC:Peri mg/m^3 0.27E-01 0.27E-01 22 HPLC:Pheide-a mg/m^3 0 0 23 HPLC:But-fuco mg/m^3 0.23E-01 0.23E-01 24 HPLC:Fuco mg/m^3 2.055 2.055 25 HPLC:Neo mg/m^3 0.2E-01 0.2E-01 26 HPLC:Pras mg/m^3 0 0 27 HPLC:Viola mg/m^3 0.13E-01 0.13E-01 28 HPLC:Hex-fuco mg/m^3 0.19E-01 0.19E-01 29 HPLC:Diadino mg/m^3 0.236 0.236 30 HPLC:Allo mg/m^3 0.74E-01 0.74E-01 31 HPLC:Diato mg/m^3 0.7E-01 0.7E-01 32 HPLC:Zea mg/m^3 0.9E-02 0.9E-02 33 HPLC:Lut mg/m^3 0.4E-02 0.4E-02 34 HPLC:Gyr-de mg/m^3 0 0 35 HPLC:Chl-b mg/m^3 0.79E-01 0.79E-01 36 HPLC:c2-MGDG mg/m^3 0 0 37 HPLC:DVChl-a mg/m^3 0 0 38 HPLC:Chl-a mg/m^3 3.178 3.178 39 HPLC:Phe mg/m^3 0 0 40 HPLC:B-Car mg/m^3 0.45E-01 0.45E-01 41 HPLC:TChl-a mg/m^3 3.272 3.272 42 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 9 F R4 4 5 -99 ' ' 9 F R4 4 6 -99 ' ' 8 F R4 3 7 -99 ' ' 6 F R4 1 8 -99 ' ' 8 F R4 3 9 -99 ' ' 8 F R4 3 10 -99 ' ' 10 F R4 5 11 -99 ' ' 6 F ' ' 0 12 -99 ' ' 7 F R4 2 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 0 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2018-96 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. PROJECT : Zooplankton - Strait of Georgia SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION STATION : 2 EVENT NUMBER : 8 LATITUDE : 49 24.12000 N ! (deg min) LONGITUDE : 124 9.24000 W ! (deg min) WATER DEPTH : 280 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0404 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.1 2018/05/24 12:47:09 1 1 CLEAN 5.2.2 2018/05/24 12:47:17 1 1 REMOVECH 8.2 2018/05/24 12:47:21 1 1 SORT 3.6 2018/05/24 12:47:23 1 1 HDREDIT2 3.1.1 2018/05/24 12:47:33 ? ? HDREDIT2 3.2 2020/01/22 14:20:16 ? ? ADDCHANS 1.4 2021/03/16 10:09:56 ? ? REORDER 1.3.1 2021/03/16 10:31:34 ? ? REORDER 1.3.1 2021/03/16 10:47:39 ? ? CLEAN 5.2.4 2021/03/16 11:02:25 1 1 CLEAN 5.2.4 2021/03/17 12:58:06 1 1 HDREDIT2 3.2 2022/06/06 12:01:43 ? ? SORT 3.6 2023/04/04 10:43:07 1 1 MERGE 3.6 2023/04/04 10:44:43 1 1 SORT 3.6 2023/04/04 10:45:12 1 1 CLEAN 5.3 2023/04/04 10:45:25 1 1 HDREDIT2 3.2 2023/04/04 10:45:54 ? ? HDREDIT2 3.2 2023/04/04 10:48:55 ? ? HDREDIT2 3.2 2025/02/13 09:48:52 ? ? $END $REMARKS -CLEAN functions: 2018/05/24 12:47:16 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Delete Empty Channels: 21 deleted. Set header Start and End times from the data. -REMOVECH 2018/05/24 12:47:21 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2018/05/24 12:47:23 Sorted in ascending order of channel Depth:Nominal [metres] -HEADER EDITS: 2018/05/24 12:47:33 Applied edit header: P:\Cruise_Data_Processing\2018-96\Processing\doc\HYDRO\2018-96-bot-hdr.txt Channel 2: Depth:Nominal [metres] Format: F6.0 ==> I6 -HEADER EDITS: 2020/01/22 14:20:16 - none. -CLEAN functions: 2021/03/16 11:02:25 20 Reset #RECS, MIN & MAX values in header. -CLEAN functions: 2021/03/17 12:58:06 Reset #RECS, MIN & MAX values in header. -HEADER EDITS: 2022/06/06 12:01:43 - none. -SORT parameters: 2023/04/04 10:43:07 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2023/04/04 10:44:43 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Gyr-de [ ], HPLC:Chl-b [ ], HPLC:C2mgdg [ ], HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [] Primary file : C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-096\Processing\IOS\ 2018-096-0008.srt1 Secondary file: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-096\Processing\IOS\ 2018-096-0008.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/04/04 10:45:12 Sorted in ascending order of channel Pressure:CTD [decibar] -CLEAN functions: 2023/04/04 10:45:25 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/04/04 10:45:54 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-096\2018-096- hdr1.txt Channel 15: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:C2mgdg [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 42: Flag:HPLC [n/a] Units: ==> n/a Channel 34: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 Channel 13: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a -HEADER EDITS: 2023/04/04 10:48:55 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-096\ Processing\Doc\2018-096-hdr2.txt -HEADER EDITS: 2025/02/13 09:48:52 Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 6: Oxygen:Dissolved [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved Format: F7.2 ==> F8.3 Channel 7: Oxygen:Dissolved [umol/kg] Name: Oxygen:Dissolved:Mass:CTD ==> Oxygen:Dissolved Channel 8: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:URU:CTD ==> Fluorescence:URU:Wetlabs Channel 10: Conductivity:Primary [S/m] Format: F10.6 ==> F10.5 Channel 36: HPLC:c2-MGDG [mg/m^3] Name: HPLC:C2mgdg ==> HPLC:c2-MGDG Channel 42: Flag:HPLC [n/a] Pad: -99 ==> 0 Channel 11: Depth:Nominal [metres] Format: I6 ==> F6.0 $END *COMMENTS Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials in liquid nitrogen (onboard) and then at -80C (at IOS) prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~3 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 1.23-7.51ug/l. Average %CV for this cruise was 2.62% with 0 out of 11 duplicate pairs having a CV>10% and 0 out of 11 duplicate pairs having a CV>30%. Our average dataset %CV is 3.73 % for 2013 - 2017 so the overall quality of this dataset is excellent. For details see worksheet “CV%” in file QF2018-96CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6 weeks after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2018-096HPLC*.xlsx. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in a -20C freezer. All analysis was performed on frozen samples. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke, J. and F. Whitney. For details see document NUTS_QF2018-96*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 13 to 17 days days after collection. For details, see document QF2018-96SAL*.xls. Dissolved inorganic carbon (DIC) was determined using coulometric analysis. Total alkalinity (Alk) was determined by potentiometric titration. Complete documentation on DIC, ALK analysis and precision estimates can be found in in the Metadata section of document QF2018-96_DIC*.xlsx. Note that samples with nominal depth 0 were surface samples with no matching CTD data, so the shallowest available CTD data were chosen. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 3. A. Dickson, C. Goyet, Handbook of Methods for the Analysis of the Various Parameters of the Carbon Dioxide System in Sea Water, September 1994, U.S. Department of Energy 4. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- The CTD data in these files were extracted from CTD profile files after the recalibration of the CTD dissolved oxygen channels. Fluorescence, Dissolved Oxygen and pH:SBE data are nominal and unedited, except that some records were removed in editing temperature and salinity. Channel Fluorescence:URU:Wetlabs had some very small negative values in deep water which were set to 0 using program Calibrate. There were 8 deep salinity bottle samples during this cruise. The CTD salinity values were higher than the bottles by a median value of 0.0031psu which is within the expected accuracy. Some of this difference may be due to the fact that flushing of Niskin bottles is somewhat inefficient so that the water in the bottles may have come from slightly higher in the water column. A comparison with an SBE911+ cast during a previous cruise also showed that the salinity was likely within expected accuracy. No recalibration was applied to the CTD salinity. There was no dissolved oxygen sampling during this cruise or previous cruises using this equipment in 2017. An inter-ship comparison of 2 casts from a June 2017 cruise using these sensors with a cast from a cruise that did include calibration sampling, suggested that dissolved oxygen values were likely low by up to 4%. A comparison with an SBE911+ cast at the same site during a recent cruise had a lot of scatter but suggested that dissolved oxygen was reading low by at least 5%. Channel Oxygen:Dissolved:SBE was recalibrated by adding 5% to all values. WARNING: The pH:SBE:Nominal data should be used with caution; no field calibration data were available at the time of processing. Calibration is required for each cast to get absolute values, although general trends within a cast are likely real. For details on the processing see processing report: 2018-96-Processing_Report.doc. --------------------------------------------------------------------------------- !-1-- --2--- --3--- ---4---- ---5---- ---6--- --7-- ---8--- ---9--- ----10--- --11- --12-- 13 --14-- ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- 42 !Samp Pressu Depth Temperat Salinity Oxygen: Oxyge Fluores pH:SBE: Conductiv Depth Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re ure: :CTD Dissolv n: cence: Nominal ity: : phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Phe Car TChl-a ag !Numb Primary ed Disso URU: Primary Nomin Extrac ~a t: a chlide a fuco fuco : !er lved Wetlabs al ted ct Extrac HP ! ed ted LC !---- ------ ------ -------- -------- ------- ----- ------- ------- --------- ----- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 11 2.0 2.0 6.9400 27.8322 6.990 305.4 1.148 8.837 2.86425 3. 2.00 .6 5.00 0.166 0.011 0.032 0.325 0.113 0.084 0.027 0.000 0.023 2.055 0.020 0.000 0.013 0.019 0.236 0.074 0.070 0.009 0.004 0.000 0.079 0.000 0.000 3.178 0.000 0.045 3.272 6