*2024/04/17 11:48:08.77 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2018/07/19 23:13:00.000 TIME ZERO : UTC 1980/01/01 00:00:00.000 NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 41 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 45 45 2 Pressure decibar 1.2 1.2 3 Depth metres 1.2 1.2 4 Depth:Nominal metres 0 0 5 Temperature:Primary 'deg C (ITS90)' 18.2415 18.2415 6 Conductivity:Primary S/m 3.361825 3.361825 7 Fluorescence:URU:Wetlabs mg/m^3 4.72 4.72 8 Salinity:T0:C0 PSS-78 24.6054 24.6054 9 Oxygen:Dissolved:SBE mL/L 6.59 6.59 10 Oxygen:Dissolved:SBE umol/kg 289.2 289.2 11 Chlorophyll:Extracted mg/m^3 2.33 2.33 12 Flag:Chlorophyll:Extracted n/a 13 Phaeo-Pigment:Extracted mg/m^3 0.77 0.77 14 HPLC:Chl-c3 mg/m^3 0.5E-01 0.5E-01 15 HPLC:Chlide-a mg/m^3 0 0 16 HPLC:MgDVP mg/m^3 0.3E-01 0.3E-01 17 HPLC:Chl-c2 mg/m^3 0.116 0.116 18 HPLC:Chl-c1 mg/m^3 0 0 19 HPLC:Me-chlide mg/m^3 0 0 20 HPLC:Peri mg/m^3 0 0 21 HPLC:Pheide-a mg/m^3 0 0 22 HPLC:But-fuco mg/m^3 0.2E-01 0.2E-01 23 HPLC:Fuco mg/m^3 0.184 0.184 24 HPLC:Neo mg/m^3 0.39E-01 0.39E-01 25 HPLC:Pras mg/m^3 0.111 0.111 26 HPLC:Viola mg/m^3 0.29E-01 0.29E-01 27 HPLC:Hex-fuco mg/m^3 0.57E-01 0.57E-01 28 HPLC:Diadino mg/m^3 0.68E-01 0.68E-01 29 HPLC:Allo mg/m^3 0.287 0.287 30 HPLC:Diato mg/m^3 0.27E-01 0.27E-01 31 HPLC:Zea mg/m^3 0.186 0.186 32 HPLC:Lut mg/m^3 0.41E-01 0.41E-01 33 HPLC:Gyr-de mg/m^3 0 0 34 HPLC:Chl-b mg/m^3 0.364 0.364 35 HPLC:C2mgdg mg/m^3 0 0 36 HPLC:DVChl-a mg/m^3 0 0 37 HPLC:Chl-a mg/m^3 2.235 2.235 38 HPLC:Phe mg/m^3 0 0 39 HPLC:B-Car mg/m^3 0.47E-01 0.47E-01 40 HPLC:TChl-a mg/m^3 2.235 2.235 41 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 7 F R4 1 5 -99 ' ' 9 F R4 4 6 -99 ' ' 11 F R4 6 7 -99 ' ' 8 F R4 3 8 -99 ' ' 9 F R4 4 9 -99 ' ' 7 F R4 2 10 -99 ' ' 6 F R4 1 11 -99 ' ' 7 F R4 2 12 -99 ' ' 3 NQ C ' ' 13 -99 ' ' 7 F R4 2 14 -99 ' ' 8 F R4 3 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2018-037 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 27 EVENT NUMBER : 49 LATITUDE : 49 19.11180 N ! (deg min) LONGITUDE : 123 47.99580 W ! (deg min) WATER DEPTH : 339 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0404 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.2 2018/11/01 11:40:30 1 1 CLEAN 5.2.3 2018/11/01 11:44:18 1 1 REMOVECH 8.2 2018/11/01 11:44:57 1 1 CHGUNITS 3.1.1 2018/11/01 11:45:26 1 1 REORDER 1.3.1 2018/11/01 11:51:10 ? ? SORT 3.6 2018/11/01 11:51:18 1 1 HDREDIT2 3.1.1 2018/11/01 11:51:23 ? ? HDREDIT2 3.2 2020/01/22 14:25:06 ? ? HDREDIT2 3.2 2020/06/08 11:14:38 ? ? CLEAN 5.2.4 2021/04/14 13:39:50 1 1 SORT 3.6 2023/03/27 15:17:22 1 1 REORDER 1.3.1 2023/03/27 15:17:58 ? ? MERGE 3.6 2023/03/27 15:18:17 1 1 SORT 3.6 2023/03/27 15:18:34 1 1 CLEAN 5.3 2023/03/27 15:18:40 1 1 HDREDIT2 3.2 2023/03/27 15:21:35 ? ? HDREDIT2 3.2 2023/03/27 15:22:18 ? ? CLEAN 5.3 2023/03/28 10:14:20 1 1 HDREDIT2 3.2 2024/04/17 11:48:08 ? ? $END $REMARKS -CLEAN functions: 2018/11/01 11:44:17 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Delete Empty Channels: 11 deleted. Set header Start and End times from the data. -REMOVECH 2018/11/01 11:44:57 The following CHANNEL(S) were removed: Date TIME:UTC -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:CTD - No units changed -SORT parameters: 2018/11/01 11:51:18 Sorted in ascending order of channel Depth:Nominal [metres] -HEADER EDITS: 2018/11/01 11:51:23 Applied edit header: P:\Cruise_Data_Processing\2018-037\Processing\doc\HYDRO\2018-037-bot-hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F9.4 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 6: Oxygen:Dissolved:SBE [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Format: F9.4 ==> F7.2 Channel 7: Oxygen:Dissolved:SBE [umol/kg] Name: Oxygen:Dissolved:Mass:CTD ==> Oxygen:Dissolved:SBE Channel 8: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs Channel 10: Depth:Nominal [metres] Format: F6.0 ==> F7.1 -HEADER EDITS: 2020/01/22 14:25:06 - none. -HEADER EDITS: 2020/06/08 11:14:38 Applied edit header: Q:\OSD_DataProcessing\Cruise_Data\Archive-updates\2018-037\Bottle\2018-037- hdr-fix.txt Channel 6: Oxygen:Dissolved:SBE [mL/L] Format: F7.2 ==> F8.3 -CLEAN functions: 2021/04/14 13:39:50 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Set event to last 4 characters of file name -SORT parameters: 2023/03/27 15:17:22 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2023/03/27 15:18:17 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Gyr-de [ ], HPLC:Chl-b [ ], HPLC:C2mgdg [ ], HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [] Primary file : C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-037\Processing\IOS\ 2018-037-0049.che2 Secondary file: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-037\Processing\IOS\ 2018-037-0049.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/27 15:18:34 Sorted in ascending order of channel Sample_Number [n/a] -CLEAN functions: 2023/03/27 15:18:40 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 15:21:35 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-037\2018-037- hdr1.txt Channel 14: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 15: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:C2mgdg [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 41: Flag:HPLC [n/a] Units: ==> n/a Channel 33: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 Channel 12: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a -HEADER EDITS: 2023/03/27 15:22:18 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-037\2018-037- hdr2.txt -CLEAN functions: 2023/03/28 10:14:20 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2024/04/17 11:48:08 Channel 6: Conductivity:Primary [S/m] Format: F10.6 ==> F11.6 $END *COMMENTS Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in cryovials in liquid nitro Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a standa Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~4 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.96-14.26ug/l. Average %CV for this cruise was 3.5% with 0 out of 8 duplicate pairs having a CV>10% and 0 out of 8 duplicate pairs having a CV>30%. Our average dataset %CV is 3.73% for 2013 - 2017 so the overall quality of this dataset is excellent. For details see worksheet “CV%” in file QF2018-037CHL*.xls. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~1 week after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2018-037HPLC*.xlsx. Oxygen samples were analyzed in a lab at the Institute of Ocean Sciences using an automated Winkler titration System (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details see document QF2018-037OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 33 - 37 days after collection. For details including a duplicate analysis, see document QF2018-037SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2018-037nuts*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- Conductivity and Fluorescence:URU:Wetlabs are nominal and unedited except that some records were removed in editing temperature and salinity. Salinity samples were taken at the bottom of a few CTD casts and during 3 hydro casts with 8 bottles each. There are CTD data collected before 2 of the hydro casts. The CTD was found to be higher than bottle samples by approximately 0.007psu but incomplete flushing of Niskin bottles is likely so the CTD salinity may be closer to, or even lower than, in situ conditions. The accuracy of the CTD salinity is likely within +/-0.005psu. No recalibration was applied. There was dissolved oxygen sampling for this cruise, but there was too much scatter in the comparison with CTD DO and too few samples to enable a justifiable method for excluding outliers. Many samples were flagged 3 by the analyst and the range of DO was limited. The comparison suggested a correction of between 4% and 11%. There was an earlier cruise with a comparison to a CTD911+ cast that suggested that the CTD DO should be increased by 6%. While that comparison was also of limited quality, it looks more reliable, so the 6% correction was applied. Warning: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. For details on the processing see document: 2018-037_Processing_Report.doc. --------------------------------------------------------------------------------- !-1-- --2--- --3--- --4--- ---5---- ----6----- ---7--- ---8---- --9--- --10- --11-- 12 --13-- ---14-- ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- 41 !Samp Pressu Depth Depth: Temperat Conductivi Fluores Salinity Oxygen Oxyge Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re Nomina ure: ty:Primary cence: :T0:C0 : n: phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b C2mgdg DVChl-a Chl-a Phe Car TChl-a ag !Numb l Primary URU: Dissol Disso Extrac ~a t: a chlide a fuco fuco : !er Wetlabs ved: lved: ted ct Extrac HP ! SBE SBE ed ted LC !---- ------ ------ ------ -------- ---------- ------- -------- ------ ----- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 45 1.2 1.2 0.0 18.2415 3.361825 4.720 24.6054 6.59 289.2 2.33 6 0.77 0.050 0.000 0.030 0.116 0.000 0.000 0.000 0.000 0.020 0.184 0.039 0.111 0.029 0.057 0.068 0.287 0.027 0.186 0.041 0.000 0.364 0.000 0.000 2.235 0.000 0.047 2.235 6