*2025/02/26 16:14:58.08 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2018/05/27 10:45:00.000 TIME ZERO : UTC 1980/01/01 00:00:00.000 NUMBER OF RECORDS : 2 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 42 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 9 10 2 Pressure decibar 1.3 10 3 Depth metres 1.3 5 4 Depth:Nominal metres 0 5 5 Temperature:Primary 'deg C (ITS90)' 14.8655 15.1499 6 Conductivity:Primary S/m 2.037789 2.125773 7 Fluorescence:URU:Wetlabs mg/m^3 4.659 6.091 8 pH:SBE:Nominal n/a 9.194 9.273 9 Salinity:T0:C0 PSS-78 15.3339 16.1759 10 Oxygen:Dissolved:SBE mL/L 6.87 7.52 11 Oxygen:Dissolved:SBE umol/kg 303.2 332.1 12 Chlorophyll:Extracted mg/m^3 11.42 11.42 13 Flag:Chlorophyll:Extracted n/a 14 Phaeo-Pigment:Extracted mg/m^3 2.16 2.16 15 HPLC:Chl-c3 mg/m^3 0.29E-01 0.29E-01 16 HPLC:Chlide-a mg/m^3 0.104 0.104 17 HPLC:MgDVP mg/m^3 0.51E-01 0.51E-01 18 HPLC:Chl-c2 mg/m^3 0.904 0.904 19 HPLC:Chl-c1 mg/m^3 0.222 0.222 20 HPLC:Me-chlide mg/m^3 0.34E-01 0.34E-01 21 HPLC:Peri mg/m^3 0.917 0.917 22 HPLC:Pheide-a mg/m^3 0 0 23 HPLC:But-fuco mg/m^3 0.41E-01 0.41E-01 24 HPLC:Fuco mg/m^3 3.109 3.109 25 HPLC:Neo mg/m^3 0.125 0.125 26 HPLC:Pras mg/m^3 0 0 27 HPLC:Viola mg/m^3 0.51 0.51 28 HPLC:Hex-fuco mg/m^3 0 0 29 HPLC:Diadino mg/m^3 0.867 0.867 30 HPLC:Allo mg/m^3 0.345 0.345 31 HPLC:Diato mg/m^3 0.351 0.351 32 HPLC:Zea mg/m^3 0.223 0.223 33 HPLC:Lut mg/m^3 0.183 0.183 34 HPLC:Gyr-de mg/m^3 0 0 35 HPLC:Chl-b mg/m^3 0.645 0.645 36 HPLC:c2-MGDG mg/m^3 0 0 37 HPLC:DVChl-a mg/m^3 0 0 38 HPLC:Chl-a mg/m^3 11.705 11.705 39 HPLC:Phe mg/m^3 0 0 40 HPLC:B-Car mg/m^3 0.287 0.287 41 HPLC:TChl-a mg/m^3 11.843 11.843 42 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 7 F R4 1 5 -99 ' ' 9 F R4 4 6 -99 ' ' 10 F R4 6 7 -99 ' ' 8 F R4 3 8 -99 ' ' 8 F R4 3 9 -99 ' ' 9 F R4 4 10 -99 ' ' 7 F R4 2 11 -99 ' ' 6 F R4 1 12 -99 ' ' 7 F R4 2 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2018-036 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 28 EVENT NUMBER : 13 LATITUDE : 49 24.12480 N ! (deg min) LONGITUDE : 123 45.33180 W ! (deg min) WATER DEPTH : 135 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0404 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.2 2018/10/19 16:39:57 2 2 CLEAN 5.2.3 2018/10/22 08:27:51 2 2 REMOVECH 8.2 2018/10/22 08:28:15 2 2 SORT 3.6 2018/10/22 08:28:20 2 2 HDREDIT2 3.1.1 2018/10/22 10:13:40 ? ? HDREDIT2 3.2 2020/01/22 14:18:58 ? ? CLEAN 5.2.4 2021/03/17 10:47:30 2 2 CLEAN 5.2.4 2021/04/14 13:43:22 2 2 SORT 3.6 2023/03/27 14:17:21 2 2 REORDER 1.3.1 2023/03/27 14:17:40 ? ? MERGE 3.6 2023/03/27 14:18:16 2 2 SORT 3.6 2023/03/27 14:18:31 2 2 CLEAN 5.3 2023/03/27 14:18:40 2 2 HDREDIT2 3.2 2023/03/27 14:19:05 ? ? HDREDIT2 3.2 2023/03/27 14:19:20 ? ? CLEAN 5.3 2023/03/28 10:13:11 2 2 HDREDIT2 3.2 2025/02/26 16:14:58 ? ? $END $REMARKS -CLEAN functions: 2018/10/22 08:27:51 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Delete Empty Channels: 2 deleted. Set header Start and End times from the data. -REMOVECH 2018/10/22 08:28:15 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2018/10/22 08:28:20 Sorted in ascending order of channel Depth:Nominal [metres] -HEADER EDITS: 2018/10/22 10:13:40 Applied edit header: P:\Cruise_Data_Processing\2018-036\Processing\doc\HYDRO\2018-036-bot-hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F9.4 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 6: Oxygen:Dissolved:SBE [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Format: F9.4 ==> F7.2 Channel 7: Oxygen:Dissolved:SBE [umol/kg] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Channel 8: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs Channel 11: Depth:Nominal [metres] Format: F6.0 ==> F7.1 -HEADER EDITS: 2020/01/22 14:18:58 - none. -CLEAN functions: 2021/03/17 10:47:29 20 Reset #RECS, MIN & MAX values in header. -CLEAN functions: 2021/04/14 13:43:22 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Set event to last 4 characters of file name -SORT parameters: 2023/03/27 14:17:21 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2023/03/27 14:18:16 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Gyr-de [ ], HPLC:Chl-b [ ], HPLC:C2mgdg [ ], HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [] Primary file : C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-036\Processing\IOS\ 2018-036-0013.che2 Secondary file: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-036\Processing\IOS\ 2018-036-0013.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/27 14:18:31 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/27 14:18:40 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 14:19:05 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-036\2018-036- hdr1.txt Channel 15: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:C2mgdg [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 42: Flag:HPLC [n/a] Units: ==> n/a Channel 34: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 Channel 13: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a -HEADER EDITS: 2023/03/27 14:19:20 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-036\2018-036- hdr2.txt -CLEAN functions: 2023/03/28 10:13:11 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2025/02/26 16:14:58 Channel 36: HPLC:c2-MGDG [mg/m^3] Name: HPLC:C2mgdg ==> HPLC:c2-MGDG $END *COMMENTS Analysis methods: ----------------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in cryovials in liquid nitro Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a standa Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~6 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Chlorophyll values ranged from 0.60 to 11.42ug/l. Average %CV for this cruise was 5.57 with 0 out of 3 duplicate pairs having a CV>10% and 0 out of 3 duplicate pairs having a CV>30%. Our average dataset %CV is 3.73% for 2013-2017 so the overall quality of this dataset is good. For details see worksheet “CV%” in file QF2018-36 CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 weeks after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2018-036HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 21 to 26 days days after collection. For details, see document QF2018-036SAL*.xls. Note that samples with nominal depth 0 were surface samples with no matching CTD data, so the shallowest available CTD data were chosen. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 3. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: ---------------------------- Conductivity, Fluorescence:URU:Wetlabs and pH:SBE data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 6 deep salinity bottle samples from this cruise. The CTD salinity values were higher than the bottles by a median value of 0.002psu. However, incomplete flushing of Niskin bottles may lead to bottle values being lower than in situ values. Thus the CTD may look like it is reading higher than it really is. The CTD salinity data are considered +/- 0.003psu which is well within the expected accuracy for this equipment. No recalibration was applied to the CTD salinity. There was no dissolved oxygen sampling during this cruise or previous cruises using this equipment in 2017. An inter-ship comparison of 2 casts from a June 2017 cruise using these sensors with a cast from a cruise that did include calibration sampling, suggested that dissolved oxygen values were likely low by up to 4%. A comparison with an SBE911+ cast at the same site during a February 2018 cruise had a lot of scatter but suggested that dissolved oxygen was reading low low by at least 5%. Channel Oxygen:Dissolved:SBE was recalibrated by adding 5% to all values. WARNING: The pH:SBE:Nominal data should be used with caution; no field calibration data were available at the time of processing. Calibration is required for each cast to get absolute values, although general trends within a cast are likely real. Warning: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. For details on the processing see document: 2018-036_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-036\Processing\IOS\2018-036-0013. hplc --------------------------------------------------------------------------- Sample_Number 9: HPLC: >10%CV for TChl-a !-1-- --2--- --3--- --4--- ---5---- ----6---- ---7--- ---8--- ---9---- --10-- --11- --12-- 13 --14-- ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- 42 !Samp Pressu Depth Depth: Temperat Conductiv Fluores pH:SBE: Salinity Oxygen Oxyge Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re Nomina ure: ity: cence: Nominal :T0:C0 : n: phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b c2-MGDG DVChl-a Chl-a Phe Car TChl-a ag !Numb l Primary Primary URU: Dissol Disso Extrac ~a t: a chlide a fuco fuco : !er Wetlabs ved: lved: ted ct Extrac HP ! SBE SBE ed ted LC !---- ------ ------ ------ -------- --------- ------- ------- -------- ------ ----- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 9 1.3 1.3 0.0 15.1499 2.037789 4.659 9.273 15.3339 7.52 332.1 11.42 2 2.16 0.029 0.104 0.051 0.904 0.222 0.034 0.917 0.000 0.041 3.109 0.125 0.000 0.510 0.000 0.867 0.345 0.351 0.223 0.183 0.000 0.645 0.000 0.000 11.705 0.000 0.287 11.843 36 10 10.0 5.0 5.0 14.8655 2.125773 6.091 9.194 16.1759 6.87 303.2 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0