*2023/03/28 10:11:56.39 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2018/04/29 15:40:00.000 TIME ZERO : UTC 1980/01/01 00:00:00.000 NUMBER OF RECORDS : 4 DATA DESCRIPTION : Bottle:Wire + CTD:Down FILE TYPE : ASCII NUMBER OF CHANNELS : 44 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 13 16 2 Pressure decibar 0.5 337 3 Depth metres 0.5 333.9 4 Depth:Nominal metres 0 337 5 Temperature:Primary 'deg C (ITS90)' 9.5456 10.9371 6 Conductivity:Primary S/m 3.12113 3.394327 7 Fluorescence:URU:Wetlabs mg/m^3 0.69E-01 4.569 8 pH:SBE:Nominal n/a 8.292 9.101 9 Salinity:T0:C0 PSS-78 27.3471 31.0398 10 Oxygen:Dissolved:SBE mL/L 1.75 6.45 11 Oxygen:Dissolved:SBE umol/kg 76.1 281.9 12 Salinity:Bottle PSS-78 31.0426 31.0426 13 Flag:Salinity:Bottle n/a 14 Chlorophyll:Extracted mg/m^3 6.06 6.06 15 Flag:Chlorophyll:Extracted n/a 16 Phaeo-Pigment:Extracted mg/m^3 1.32 1.32 17 HPLC:Chl-c3 mg/m^3 0.362 0.362 18 HPLC:Chlide-a mg/m^3 0.95E-01 0.95E-01 19 HPLC:MgDVP mg/m^3 0.26E-01 0.26E-01 20 HPLC:Chl-c2 mg/m^3 0.653 0.653 21 HPLC:Chl-c1 mg/m^3 0.81E-01 0.81E-01 22 HPLC:Me-chlide mg/m^3 0.408 0.408 23 HPLC:Peri mg/m^3 0.102 0.102 24 HPLC:Pheide-a mg/m^3 0 0 25 HPLC:But-fuco mg/m^3 0.35 0.35 26 HPLC:Fuco mg/m^3 1.833 1.833 27 HPLC:Neo mg/m^3 0.51E-01 0.51E-01 28 HPLC:Pras mg/m^3 0.2E-01 0.2E-01 29 HPLC:Viola mg/m^3 0.21E-01 0.21E-01 30 HPLC:Hex-fuco mg/m^3 0.29E-01 0.29E-01 31 HPLC:Diadino mg/m^3 0.258 0.258 32 HPLC:Allo mg/m^3 0.28 0.28 33 HPLC:Diato mg/m^3 0.42E-01 0.42E-01 34 HPLC:Zea mg/m^3 0.59E-01 0.59E-01 35 HPLC:Lut mg/m^3 0 0 36 HPLC:Gyr-de mg/m^3 0 0 37 HPLC:Chl-b mg/m^3 0.179 0.179 38 HPLC:C2mgdg mg/m^3 0 0 39 HPLC:DVChl-a mg/m^3 0 0 40 HPLC:Chl-a mg/m^3 4.021 4.021 41 HPLC:Phe mg/m^3 0 0 42 HPLC:B-Car mg/m^3 0.4E-01 0.4E-01 43 HPLC:TChl-a mg/m^3 4.524 4.524 44 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 7 F R4 1 3 -99 ' ' 7 F R4 1 4 -99 ' ' 7 F R4 1 5 -99 ' ' 9 F R4 4 6 -99 ' ' 10 F R4 6 7 -99 ' ' 8 F R4 3 8 -99 ' ' 8 F R4 3 9 -99 ' ' 9 F R4 4 10 -99 ' ' 7 F R4 2 11 -99 ' ' 6 F R4 1 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 3 NQ C ' ' $END $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment 3 = Questionable measurement (Probably Good) 4 = Poor measurement (Probably Bad) 5 = Measurement not reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2018-035 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Strait of Georgia Zooplankton SCIENTIST : Young K. PLATFORM : Neocaligus *LOCATION GEOGRAPHIC AREA : Strait of Georgia STATION : 22 EVENT NUMBER : 24 LATITUDE : 49 40.16880 N ! (deg min) LONGITUDE : 124 16.39620 W ! (deg min) WATER DEPTH : 351 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-25 SERIAL NUMBER : 0404 *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SPRD2IS 5.2 2018/10/17 16:26:28 4 4 CLEAN 5.2.3 2018/10/17 16:28:04 4 4 REMOVECH 8.2 2018/10/17 16:28:40 4 4 SORT 3.6 2018/10/17 16:28:50 4 4 HDREDIT2 3.1.1 2018/10/18 09:02:30 ? ? HDREDIT2 3.2 2020/01/22 14:17:53 ? ? CLEAN 5.2.4 2021/03/17 10:22:59 4 4 CLEAN 5.2.4 2021/04/14 13:36:06 4 4 SORT 3.6 2023/03/27 13:38:29 4 4 REORDER 1.3.1 2023/03/27 13:57:40 ? ? MERGE 3.6 2023/03/27 13:58:03 4 4 SORT 3.6 2023/03/27 13:58:10 4 4 CLEAN 5.3 2023/03/27 13:58:18 4 4 HDREDIT2 3.2 2023/03/27 13:58:39 ? ? HDREDIT2 3.2 2023/03/27 13:58:55 ? ? CLEAN 5.3 2023/03/28 10:11:56 4 4 $END $REMARKS -CLEAN functions: 2018/10/17 16:28:04 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Delete Empty Channels: 0 deleted. Set header Start and End times from the data. -REMOVECH 2018/10/17 16:28:40 The following CHANNEL(S) were removed: Date TIME:UTC -SORT parameters: 2018/10/17 16:28:50 Sorted in ascending order of channel Depth:Nominal [metres] -HEADER EDITS: 2018/10/18 09:02:30 Applied edit header: P:\Cruise_Data_Processing\2018-035\Processing\doc\HYDRO\2018-035-bot-hdr.txt Channel 2: Pressure [decibar] Name: Pressure:CTD ==> Pressure Format: F9.4 ==> F7.1 Channel 3: Depth [metres] Name: Depth:CTD ==> Depth Channel 4: Temperature:Primary [deg C (ITS90)] Name: Temperature:CTD ==> Temperature:Primary Channel 5: Salinity:T0:C0 [PSS-78] Name: Salinity:CTD ==> Salinity:T0:C0 Channel 6: Oxygen:Dissolved:SBE [mL/L] Name: Oxygen:Dissolved:Volume:CTD ==> Oxygen:Dissolved:SBE Format: F9.4 ==> F7.2 Channel 7: Oxygen:Dissolved:SBE [umol/kg] Name: Oxygen:Dissolved:Mass:CTD ==> Oxygen:Dissolved:SBE Format: F7.2 ==> F6.1 Channel 8: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:CTD ==> Fluorescence:URU:Wetlabs Channel 11: Depth:Nominal [metres] Format: F6.0 ==> F7.1 -HEADER EDITS: 2020/01/22 14:17:53 - none. -CLEAN functions: 2021/03/17 10:22:59 20 Reset #RECS, MIN & MAX values in header. -CLEAN functions: 2021/04/14 13:36:06 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Set event to last 4 characters of file name -SORT parameters: 2023/03/27 13:38:29 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2023/03/27 13:58:03 Merge Channel: Sample_Number Merge Scheme Used: 4: Add Secondary to Matching Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Gyr-de [ ], HPLC:Chl-b [ ], HPLC:C2mgdg [ ], HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [] Primary file : C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-035\Processing\IOS\ 2018-035-0024.che2 Secondary file: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-035\Processing\IOS\ 2018-035-0024.hplc 1 secondary records matched to primary records. -SORT parameters: 2023/03/27 13:58:10 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2023/03/27 13:58:18 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2023/03/27 13:58:39 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-035\2018-035- hdr1.txt Channel 17: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:C2mgdg [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 44: Flag:HPLC [n/a] Units: ==> n/a Channel 36: HPLC:Gyr-de [mg/m^3] Units: ==> mg/m^3 Channel 13: Flag:Salinity:Bottle [n/a] Units: ==> n/a Channel 15: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a -HEADER EDITS: 2023/03/27 13:58:55 Applied edit header: C:\Users\huntingtons\Desktop\HPLC notes\Jared_cruises\2018\2018-035\2018-035- hdr2.txt -CLEAN functions: 2023/03/28 10:11:56 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* $END *COMMENTS Sample_Number 15: 24 Sample_Number 16: 24 Sample_Number 13: 24 Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in cryovials in liquid n Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a st Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 ~6 weeks after the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. For details see worksheet “CV%” in file QF2018-35 CHL*.xlsx. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6 weeks after collection The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2018-035HPLC*.xlsx. Salinity samples were collected in 200 mL type II glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater within 52 to 55 days days after collection. For details, see document QF2018-035SAL*.xls. Note that samples with nominal depth 0 were surface samples with no matching CTD data, so the shallowest available CTD data were chosen. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 3. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Conductivity, Fluorescence:URU:Wetlabs and pH:SBE data are nominal and unedited except that some records were removed in editing temperature and salinity. There were 6 deep salinity bottle samples from this cruise. The CTD salinity values were lower than the bottles by a median value of 0.001psu which is well within the expected accuracy. However, delay in analysis may have led to increased bottle salinity values that would make the CTD appear to read lower than it really is. As well, incomplete flushing of Niskin bottles may lead to bottle salinity differing from in situ waters in an unpredictable way; local vertical salinity gradients were complex with some large reversals so the errors may be of variable sign. Some of these errors would be compensating. So the CTD salinity may be a little less accurate than it appears in the comparison, but is likely within +/- 0.003psu. A comparison with an SBE911+ cast during a previous cruise also showed that the salinity was likely within expected accuracy. No recalibration was applied to the CTD salinity. There was no dissolved oxygen sampling during this cruise or previous cruises using this equipment in 2017. An inter-ship comparison of 2 casts from a June 2017 cruise using these sensors with a cast from a cruise that did include calibration sampling, suggested that dissolved oxygen values were likely low by up to 4%. A comparison with an SBE911+ cast at the same site during a February 2018 cruise had a lot of scatter but suggested that dissolved oxygen was reading low low by at least 5%. Channel Oxygen:Dissolved:SBE was recalibrated by adding 5% to all values. When plotting or exporting data from these files it is recommended that you select Depth:Nominal to capture surface samples for which there is no Pressure channel. WARNING: The pH:SBE:Nominal data should be used with caution; no field calibration data were available at the time of processing. Calibration is required for each cast to get absolute values, although general trends within a cast are likely real. WARNING: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples. For details on the processing see document: 2018-035_Processing_Report.doc. --------------------------------------------------------------------------------- !-1-- --2--- --3--- --4--- ---5---- ----6---- ---7--- ---8--- ---9---- --10-- --11- ---12--- 13 --14-- 15 --16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- 44 !Samp Pressu Depth Depth: Temperat Conductiv Fluores pH:SBE: Salinity Oxygen Oxyge Salinity Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ re Nomina ure: ity: cence: Nominal :T0:C0 : n: :Bottle ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Gyr-de Chl-b C2mgdg DVChl-a Chl-a Phe Car TChl-a ag !Numb l Primary Primary URU: Dissol Disso ~o Extrac ~a t: a chlide a fuco fuco : !er Wetlabs ved: lved: tt ted ct Extrac HP ! SBE SBE le ed ted LC !---- ------ ------ ------ -------- --------- ------- ------- -------- ------ ----- -------- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 14 0.5 0.5 0.0 10.9371 3.121130 3.466 9.101 27.3471 6.45 281.9 -99.0000 0 6.06 0 1.32 0.362 0.095 0.026 0.653 0.081 0.408 0.102 0.000 0.350 1.833 0.051 0.020 0.021 0.029 0.258 0.280 0.042 0.059 0.000 0.000 0.179 0.000 0.000 4.021 0.000 0.040 4.524 6 15 5.0 5.0 5.0 10.7669 3.148341 4.569 9.046 27.7375 6.33 276.6 -99.0000 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 16 10.0 9.9 10.0 10.2195 3.133234 4.227 8.982 28.0057 6.02 263.3 -99.0000 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 13 337.0 333.9 337.0 9.5456 3.394327 0.069 8.292 31.0398 1.75 76.1 31.0426 36 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0