*2021/11/25 16:09:56.92 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2016/09/11 11:14:28.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 11 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : CA1F8DE6 NUMBER OF CHANNELS : 48 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 391 401 2 Bottle_Number n/a 1 11 3 Bottle:Firing_Sequence n/a 1 11 4 Pressure decibar 2.2 288.7 5 Temperature:Secondary 'deg C (ITS90)' 9.6584 15.3853 6 Transmissivity %/metre 30.3 52.7 7 Fluorescence:URU:Seapoint mg/m^3 0.125 2.678 8 PAR uE/m^2/sec 0 0.2 9 Salinity:T1:C1 PSS-78 27.7536 30.8361 10 Oxygen:Dissolved:SBE mL/L 2.67 6.4 11 Oxygen:Dissolved:SBE umol/kg 116.6 280.1 12 Number_of_bin_records n/a 241 241 13 Nitrate_plus_Nitrite umol/L 1.71 29.74 14 Flag:Nitrate_plus_Nitrite ' ' 15 Silicate umol/L 33.37 67.82 16 Flag:Silicate ' ' 17 Phosphate umol/L 0.541 2.739 18 Flag:Phosphate ' ' 19 Chlorophyll:Extracted mg/m^3 0.4 2.68 20 Flag:Chlorophyll:Extracted ' ' 21 Phaeo-Pigment:Extracted mg/m^3 0.56 0.93 22 HPLC:Chl-c3 mg/m^3 0.89E-01 0.89E-01 23 HPLC:Chlide-a mg/m^3 0 0 24 HPLC:MgDVP mg/m^3 0.29E-01 0.29E-01 25 HPLC:Chl-c2 mg/m^3 0.162 0.162 26 HPLC:Chl-c1 mg/m^3 0 0 27 HPLC:Me-chlide mg/m^3 0 0 28 HPLC:Peri mg/m^3 0.21E-01 0.21E-01 29 HPLC:Pheide-a mg/m^3 0 0 30 HPLC:But-fuco mg/m^3 0.85E-01 0.85E-01 31 HPLC:Fuco mg/m^3 0.423 0.423 32 HPLC:Neo mg/m^3 0.47E-01 0.47E-01 33 HPLC:Pras mg/m^3 0.103 0.103 34 HPLC:Viola mg/m^3 0.37E-01 0.37E-01 35 HPLC:Hex-fuco mg/m^3 0.2E-01 0.2E-01 36 HPLC:Diadino mg/m^3 0.55E-01 0.55E-01 37 HPLC:Allo mg/m^3 0.171 0.171 38 HPLC:Diato mg/m^3 0 0 39 HPLC:Zea mg/m^3 0.88E-01 0.88E-01 40 HPLC:Lut mg/m^3 0 0 41 HPLC:Chl-b mg/m^3 0.384 0.384 42 HPLC:C2mgdg mg/m^3 0 0 43 HPLC:DVChl-a mg/m^3 0 0 44 HPLC:Chl-a mg/m^3 2.045 2.045 45 HPLC:Phe mg/m^3 0 0 46 HPLC:B-Car mg/m^3 0.57E-01 0.57E-01 47 HPLC:TChl-a mg/m^3 2.045 2.045 48 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 7 F R4 2 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 7 F R4 2 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 8 F R4 3 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels were initialized with zeros. Non-zero values have the following significance: ---------------------------------------------------------------------------------- 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable Measurement 3 = Questionable Measurement (Probably Good) 4 = Poor Measurement (Probably Bad) 5 = Measurement Not Reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2016-62 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse Zooplankton Monitoring SCIENTIST : Yelland D. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : 11 EVENT NUMBER : 189 LATITUDE : 49 42.30000 N ! (deg min) LONGITUDE : 124 43.36000 W ! (deg min) WATER DEPTH : 295 ALTIMETER (M) : 9.62 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 443 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 4752 Conductivity ' ' 2280 'Pressure:Digiquartz with TC' ' ' 0443 Temperature:2 ' ' 2710 Conductivity:2 ' ' 2754 'Oxygen:SBE 43' ' ' 0997 Fluorometer:Seapoint ' ' 3640 'Transmissometer:WET Labs C-Star' ' ' 1185DR Altimeter ' ' 62354 PAR/Irradiance:Biospherical/Licor ' ' 70613 $END $REMARKS SOFTWARE VERSION SEASAVE V 7.26.1.8 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 3.5.2 2016/12/09 13:32:15 2651 2651 CLEAN 5.2.1 2016/12/09 13:32:45 2651 2651 ADDSAMP 3.6 2016/12/09 14:16:14 2651 2651 BINAVE 4.2 2016/12/09 14:16:32 2651 11 MERGE 3.5 2016/12/15 11:02:31 11 11 CLEAN 5.2.1 2016/12/15 11:02:47 11 11 CALIB 11.9 2016/12/23 10:44:35 11 11 SORT 3.6 2016/12/23 10:44:47 11 11 REMOVECH 8.2 2016/12/23 10:45:16 11 11 CHGUNITS 3.1.1 2016/12/23 10:46:44 11 11 CHGUNITS 3.1.1 2016/12/23 10:47:07 11 11 REORDER 1.3.1 2016/12/23 10:47:19 ? ? HDREDIT2 3.0.2 2017/01/13 10:51:27 ? ? SORT 3.6 2020/08/09 15:41:20 11 11 REORDER 1.3.1 2021/11/25 16:08:19 ? ? MERGE 3.5.1 2021/11/25 16:08:32 11 11 SORT 3.6 2021/11/25 16:08:41 11 11 CLEAN 5.2.4 2021/11/25 16:08:51 11 11 HDREDIT2 3.2 2021/11/25 16:09:25 ? ? HDREDIT2 3.2 2021/11/25 16:09:56 ? ? $END $REMARKS -CLEAN functions: 2016/12/09 13:32:22 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2016-62\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2016/12/15 11:02:31 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2016-62\Processing\hydro\2016-62-0189.samavg Secondary file: P:\Cruise_Data_Processing\2016-62\Processing\hydro\2016-62-0189.mrgcln1s Comments from secondary file: P:\Cruise_Data_Processing\2016-62\Processing\hydro\2016-62-0189.mrgcln1s ------------------------------------------------------------------------ -CLEAN functions: 2016/12/13 15:22:02 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2016/12/13 15:22:06 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2016/12/15 11:02:45 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Set event to last 4 characters of file name -CALIB parameters: 2016/12/23 10:44:35 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2016-62\Processing\doc\2016-62-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Oxygen:Dissolved:SBE mL/L 10 0.2330000E-01 0.1024800E+01 -SORT parameters: 2016/12/23 10:44:47 Sorted in ascending order of channel Pressure* -REMOVECH 2016/12/23 10:45:16 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T0:C0 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Secondary Salinity reference channel: Salinity:T1:C1 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T1:C1 - No units changed -HEADER EDITS: 2017/01/13 10:51:27 Applied edit header: P:\Cruise_Data_Processing\2016-62\Processing\doc\HYDRO\2016-62-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F9.4 ==> F7.1 Channel 7: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 9: Oxygen:Dissolved:SBE [mL/L] Format: F11.3 ==> F7.2 Channel 13: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Format: F8.3 ==> F7.2 Channel 15: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 16: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 18: Silicate [umol/L] Units: ==> umol/L Channel 20: Phosphate [umol/L] Units: ==> umol/L -SORT parameters: 2020/08/09 15:41:20 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 16:08:32 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2016\2016-062_Nov2021\Processing\ IOS\2016-062-0189.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2016\2016-062_Nov2021\Processing\ IOS\2016-062-0189.hplc -SORT parameters: 2021/11/25 16:08:41 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 16:08:49 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 16:09:25 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2016\2016_062\Processing\IOS\ 2016-062-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 22: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:C2mgdg [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 16:09:56 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2016\2016_062\Processing\IOS\ 2016-062-bot-hdr2.txt $END *COMMENTS SBE HEADER Sea-Bird SBE 9 Data File: FileName = C:\CTD Data\2016-62\2016-62-0189.hex Software Version Seasave V 7.26.1.8 Temperature SN = 4752 Conductivity SN = 2280 Number of Bytes Per Scan = 37 Number of Voltage Words = 4 Number of Scans Averaged by the Deck Unit = 1 System UpLoad Time = Sep 11 2016 11:20:46 NMEA Latitude = 49 42.30 N NMEA Longitude = 124 43.36 W NMEA UTC (Time) = Sep 11 2016 11:14:28 Store Lat/Lon Data = Append to Every Scan SBE 11plus V 5.1e number of scans to average = 1 pressure baud rate = 9600 NMEA baud rate = 4800 GPIB address = 1 advance primary conductivity 0.073 seconds advance secondary conductivity 0.073 seconds S> Mission: 2016-62 Ship: J.P. Tully Station: 11 Depth: 295 System UTC = Sep 11 2016 11:20:46 # nquan = 19 # nvalues = 2651 # units = specified # name 0 = scan: Scan Count # name 1 = bpos: Bottle Position in Carousel # name 2 = nbf: Bottles Fired # name 3 = prDM: Pressure, Digiquartz [db] # name 4 = t090C: Temperature [ITS-90, deg C] # name 5 = t190C: Temperature, 2 [ITS-90, deg C] # name 6 = c0S/m: Conductivity [S/m] # name 7 = c1S/m: Conductivity, 2 [S/m] # name 8 = CStarTr0: Beam Transmission, WET Labs C-Star [%] # name 9 = flSP: Fluorescence, Seapoint # name 10 = par: PAR/Irradiance, Biospherical/Licor # name 11 = sbeox0V: Oxygen raw, SBE 43 [V] # name 12 = altM: Altimeter [m] # name 13 = dz/dtM: Descent Rate [m/s] # name 14 = pumps: Pump Status # name 15 = sal00: Salinity, Practical [PSU] # name 16 = sal11: Salinity, Practical, 2 [PSU] # name 17 = sbeox0ML/L: Oxygen, SBE 43 [ml/l] # name 18 = flag: 0.000e+00 # interval = seconds: 0.0416667 # start_time = Sep 11 2016 11:14:28 [NMEA time, header] # bad_flag = -9.990e-29 # # # # # 4752 # 16-Dec-15 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.35917908e-003 # 6.36752535e-004 # 2.08584233e-005 # 1.76255754e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 2280 # 19-Dec-14 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.00904009e+001 # 1.35149315e+000 # -1.84720682e-003 # 1.90856808e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 0443 # 17-Dec-14 # -4.435292e+004 # -1.635794e-001 # 1.192740e-002 # 3.979900e-002 # 0.000000e+000 # 2.993627e+001 # -3.271008e-004 # 3.759160e-006 # 1.565300e-009 # 0.99991613 # -1.00000 # 0.000000e+000 # 1.282462e-002 # -8.599824e+000 # # # # # # 2710 # 16-Dec-15 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.36717385e-003 # 6.46681437e-004 # 2.34978657e-005 # 2.22502310e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 2754 # 19-Dec-14 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.05920237e+001 # 1.61356129e+000 # -1.94911294e-003 # 2.41671700e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 0997 # 17-Jan-15 # 1 # # # 0.0000 # 0.0000e+000 # 0.0000 # 0.00e+000 # 0.0000 # 0.0 # # # # 4.0785e-001 # -0.6967 # -3.5082e-003 # 1.2934e-004 # -1.8411e-006 # 2.5826e+000 # 1.92634e-004 # -4.64803e-002 # 3.6000e-002 # 1.6200 #

-3.3000e-002

#

5.0000e+003

#

1.4500e+003

# # # # # # # 3640 # # # 2 # 0.000 # # # # # # 1185DR # APR 27. 2016 # 19.3479 # -1.1028 # 0.250 # # # # # # # # # 62354 # # 15.000 # 0.000 # # # # # # # # # 70613 # 03/21/16 # 1.00000000 # 0.00000000 # 11876484560.57000000 # 1.00000000 # -0.08460813 # # # # # # # datcnv_date = Dec 09 2016 12:55:01, 7.23.2 # datcnv_in = P:\Cruise_Data_Processing\2016-62\RAW\CTD\2016-62-0189.hex P:\Crui se_Data_Processing\2016-62\Processing\doc\2016-62-ctd.xmlcon # datcnv_ox_hysteresis_correction = yes # datcnv_ox_tau_correction = yes # datcnv_bottle_scan_range_source = BL file # datcnv_scans_per_bottle = 241 END* Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423 within ~6 weeks of the cruise. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.14-35.10 ug/l. Average %CV for this cruise was 3.25% with 3 out of 127 duplicate pairs having a CV>15% and 0 out of 127 duplicate pairs having a CV>50%. Our average dataset %CV is 2.83 % for 2013 - 2015 so the overall quality of this dataset is excellent. For details see worksheet “CV%” in file QF2016-62CHL*.xls. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~3 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2016-062HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2016-62OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8410A Portasal Salinometer (S/N 58879) standardized with IAPSO standard seawater within 48 - 54 days after collection. Data are reported to the third decimal place on account of the reduced accuracy and precision from the delay in analysis and the slightly less accurate instrument (±0.001 PSU less accurate the the 8400B Autosal). For details including a duplicate analysis, see document QF2016-62SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2016-62nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. For full details including a precision study see file 2016-62NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, Bethanie A., and Peterson, Bruce J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. Dissolved oxygen was calibrated using the method described in SeaBird Application Note #64-2, June 2012 revision, except that a small offset in the fit was allowed. For details on the processing see the report: 2016-62_Processing_Report.doc. --------------------------------------------------------------------------------- Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2016\2016-062_Nov2021\Processing\IOS\2016-062-0189. hplc --------------------------------------------------------------------------- Sample_Number 401: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.233E-01 1.0248) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- --8--- ---9---- --10-- --11- -12- --13-- 14 --15-- 16 ---17-- 18 --19-- 20 --21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- 48 !Samp Bott Bott Pressu Temperat Trans Fluores PAR Salinity Oxygen Oxyge Numb Nitrat Fl Silica Fl Phospha Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: :T1:C1 : n: er_o e_ ag te ag te ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b C2mgdg DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: Dissol Disso ~bin plus_ ~t ~i ~p Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin ved: lved: _rec Nitrit ri ca ha ted ct Extrac HP ! ence t SBE SBE ords e te te te ed ted LC !---- ---- ---- ------ -------- ----- ------- ------ -------- ------ ----- ---- ------ -- ------ -- ------- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 401 11 11 2.2 15.3853 30.7 2.654 0.2 27.7586 6.40 280.1 241 1.77 0 33.37 0 0.541 0 2.68 6 0.93 0.089 0.000 0.029 0.162 0.000 0.000 0.021 0.000 0.085 0.423 0.047 0.103 0.037 0.020 0.055 0.171 0.000 0.088 0.000 0.384 0.000 0.000 2.045 0.000 0.057 2.045 36 400 10 10 5.5 15.3841 30.3 2.678 0.0 27.7536 6.38 279.4 241 1.71 0 33.41 0 0.544 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 399 9 9 10.8 15.3060 32.6 2.284 0.0 27.8236 6.26 274.1 241 2.85 0 33.61 0 0.661 0 2.25 6 0.93 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 398 8 8 20.8 13.2254 48.4 0.588 0.0 28.7010 4.93 215.6 241 18.15 0 42.76 0 1.864 0 0.40 6 0.56 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 397 7 7 30.3 10.7793 52.6 0.225 0.0 29.2937 3.42 149.5 241 26.09 6 53.72 6 2.385 6 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 396 6 6 40.3 10.2196 52.7 0.176 0.0 29.5530 3.13 136.6 241 28.32 0 59.24 0 2.549 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 395 5 5 50.5 10.4735 51.5 0.202 0.0 29.8028 3.31 144.5 241 26.69 0 55.33 0 2.442 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 394 4 4 75.6 9.8120 52.6 0.144 0.0 30.0261 2.87 125.3 241 29.74 0 57.42 0 2.605 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 393 3 3 100.7 9.6903 47.6 0.136 0.0 30.3276 2.78 121.4 241 29.50 0 60.73 0 2.661 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 392 2 2 200.6 9.6584 47.4 0.125 0.0 30.7966 2.74 119.4 241 28.36 0 65.58 0 2.682 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 391 1 1 288.7 9.6608 45.6 0.128 0.0 30.8361 2.67 116.6 241 28.31 0 67.82 0 2.739 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0