*2025/05/29 09:22:36.42 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2015/09/04 18:54:43.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 8 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : A82CDD49 NUMBER OF CHANNELS : 53 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 20 27 2 Bottle_Number n/a 1 8 3 Bottle:Firing_Sequence n/a 1 8 4 Pressure decibar 4.6 106.5 5 Temperature:Secondary 'deg C (ITS90)' 7.7085 13.9676 6 Transmissivity %/metre 43.7 60.8 7 Fluorescence:URU:Seapoint mg/m^3 0.161 2.203 8 PAR uE/m^2/sec 0 1036.6 9 Salinity:T1:C1 PSS-78 32.0324 33.5798 10 Oxygen:Dissolved:SBE mL/L 2.48 6.37 11 Oxygen:Dissolved:SBE umol/kg 107.7 277.6 12 Number_of_bin_records n/a 241 241 13 Temperature:Draw 'deg C (ITS90)' 8.5 14.6 14 Oxygen:Dissolved mL/L 2.476 6.336 15 Oxygen:Dissolved umol/kg 107.7 276.3 16 Flag:Oxygen:Dissolved n/a 17 Nitrate_plus_Nitrite umol/L 2.26 28.24 18 Flag:Nitrate_plus_Nitrite n/a 19 Silicate umol/L 4.37 47.38 20 Flag:Silicate n/a 21 Phosphate umol/L 0.548 2.326 22 Flag:Phosphate n/a 23 Ammonium umol/L 0.9E-01 1.34 24 Flag:Ammonium n/a 25 Chlorophyll:Extracted mg/m^3 1.66 2.4 26 Flag:Chlorophyll:Extracted n/a 27 Phaeo-Pigment:Extracted mg/m^3 0.43 0.71 28 HPLC:Chl-c3 mg/m^3 0.137 0.137 29 HPLC:Chlide-a mg/m^3 0.68E-01 0.68E-01 30 HPLC:MgDVP mg/m^3 0.21E-01 0.21E-01 31 HPLC:Chl-c2 mg/m^3 0.219 0.219 32 HPLC:Chl-c1 mg/m^3 0.33E-01 0.33E-01 33 HPLC:Me-chlide mg/m^3 0.54E-01 0.54E-01 34 HPLC:Peri mg/m^3 0.114 0.114 35 HPLC:Pheide-a mg/m^3 0 0 36 HPLC:But-fuco mg/m^3 0.3E-02 0.3E-02 37 HPLC:Fuco mg/m^3 0.676 0.676 38 HPLC:Neo mg/m^3 0.41E-01 0.41E-01 39 HPLC:Pras mg/m^3 0.37E-01 0.37E-01 40 HPLC:Viola mg/m^3 0.24E-01 0.24E-01 41 HPLC:Hex-fuco mg/m^3 0.28E-01 0.28E-01 42 HPLC:Diadino mg/m^3 0.328 0.328 43 HPLC:Allo mg/m^3 0.116 0.116 44 HPLC:Diato mg/m^3 0.44E-01 0.44E-01 45 HPLC:Zea mg/m^3 0.39E-01 0.39E-01 46 HPLC:Lut mg/m^3 0.21E-01 0.21E-01 47 HPLC:Chl-b mg/m^3 0.147 0.147 48 HPLC:DVChl-a mg/m^3 0 0 49 HPLC:Chl-a mg/m^3 1.914 1.914 50 HPLC:Phe-a mg/m^3 0.91E-01 0.91E-01 51 HPLC:B-Car mg/m^3 0.42E-01 0.42E-01 52 HPLC:TChl-a mg/m^3 2.036 2.036 53 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 8 F R4 3 15 -99 ' ' 6 F ' ' 1 16 0 ' ' 3 NQ C ' ' 17 -99 ' ' 7 F R4 2 18 0 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 0 ' ' 3 NQ C ' ' 21 -99 ' ' 8 F R4 3 22 0 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 0 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 0 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 0 ' ' 3 NQ C ' ' $END $REMARKS Flag channels were initialized with zeros. Non-zero values have the following significance: ---------------------------------------------------------------------------------- 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable Measurement 3 = Questionable Measurement (Probably Good) 4 = Poor Measurement (Probably Bad) 5 = Measurement Not Reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2015-21 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Yelland D. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : LB06 EVENT NUMBER : 10 LATITUDE : 48 32.21000 N ! (deg min) LONGITUDE : 125 15.56000 W ! (deg min) WATER DEPTH : 113 ALTIMETER (M) : 9.04 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 2106 Conductivity ' ' 2280 'Pressure:Digiquartz with TC' ' ' 0443 Temperature:2 ' ' 2663 Conductivity:2 ' ' 2754 'Oxygen:SBE 43' ' ' 0997 Fluorometer:Seapoint ' ' 3640 'Transmissometer:WET Labs C-Star' ' ' 1396DR PAR/Irradiance:Biospherical/Licor ' ' '4565 QSP200L4S' Altimeter ' ' 62354 $END $REMARKS SOFTWARE VERSION SEASAVE V 7.23.2 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- SBE_IOS 3.4.1 2016/01/11 11:49:21 1928 1928 CLEAN 5.2.1 2016/01/11 11:55:52 1928 1928 ADDSAMP 3.6 2016/01/11 13:05:39 1928 1928 BINAVE 4.2 2016/01/11 13:06:09 1928 8 MERGE 3.5 2016/01/11 13:48:07 8 8 CLEAN 5.2.1 2016/01/11 13:48:26 8 8 CALIB 11.8.1 2016/01/12 14:10:48 8 8 SORT 3.6 2016/01/12 15:28:42 8 8 REMOVECH 8.2 2016/01/12 15:29:58 8 8 CHGUNITS 3.1 2016/01/20 15:42:18 8 8 CHGUNITS 3.1 2016/01/20 15:43:08 8 8 REORDER 1.3.1 2016/01/20 16:51:28 ? ? HDREDIT2 3.0.2 2016/01/20 17:21:46 ? ? SORT 3.6 2020/07/17 15:41:52 8 8 REORDER 1.3.1 2020/07/17 15:42:40 ? ? MERGE 3.5.1 2020/07/21 14:05:37 8 8 SORT 3.6 2020/07/21 14:05:55 8 8 HDREDIT2 3.2 2020/07/21 14:06:08 ? ? HDREDIT2 3.2 2020/07/21 14:09:35 ? ? CLEAN 5.3 2025/05/29 09:22:36 8 8 $END $REMARKS -CLEAN functions: 2016/01/11 11:55:50 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2015-21\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Bin value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2016/01/11 13:48:07 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2015-21\Processing\hydro\2015-21-0010.samavg Secondary file: P:\Cruise_Data_Processing\2015-21\Processing\hydro\2015-21-0010.mrgcln1s Comments from secondary file: P:\Cruise_Data_Processing\2015-21\Processing\hydro\2015-21-0010.mrgcln1s ------------------------------------------------------------------------ -CLEAN functions: 2016/01/11 13:47:32 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2016/01/11 13:47:43 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2016/01/11 13:48:26 20 Reset #RECS, MIN & MAX values in header. Change character data from " " to "0" in channels Flag:* Set event to last 4 characters of file name -CALIB parameters: 2016/01/12 14:10:48 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2015-21\Processing\doc\2015-21-recal1.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 20 Oxygen:Dissolved:SBE mL/L 10 0.3090000E-01 0.1022500E+01 -SORT parameters: 2016/01/12 15:28:42 Sorted in ascending order of channel Pressure* -REMOVECH 2016/01/12 15:29:58 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Voltage:6 [volts] Voltage:7 [volts] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Secondary [deg C (ITS90)] Salinity reference channel: Salinity:T1:C1 [PSS-78] 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T1:C1 [PSS-78] 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2016/01/20 17:21:46 Applied edit header: P:\Cruise_Data_Processing\2015-21\Processing\doc\HYDRO\2015-21-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Format: F9.4 ==> F7.1 Channel 7: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 9: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 13: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Format: F7.2 ==> F6.1 Channel 14: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Format: F8.3 ==> F7.2 Channel 15: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 16: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 17: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 19: Flag:Oxygen:Dissolved [n/a] Units: ==> n/a Channel 20: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Format: F6.2 ==> F7.2 Channel 21: Flag:Nitrate_plus_Nitrite [n/a] Units: ==> n/a Channel 22: Silicate [umol/L] Units: ==> umol/L Channel 23: Flag:Silicate [n/a] Units: ==> n/a Channel 24: Phosphate [umol/L] Units: ==> umol/L Channel 25: Flag:Phosphate [n/a] Units: ==> n/a Channel 27: Flag:Ammonium [n/a] Units: ==> n/a -SORT parameters: 2020/07/17 15:41:52 Sorted in ascending order of channel Sample_number -The following MERGE parameters were used: 2020/07/21 14:05:37 Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3, HPLC:Chlide-a, HPLC:MgDVP, HPLC:Chl-c2, HPLC:Chl-c1, HPLC:Me-chlide, HPLC:Peri, HPLC:Pheide-a, HPLC:But-fuco, HPLC:Fuco, HPLC:Neo, HPLC:Pras, HPLC:Viola, HPLC:Hex-fuco, HPLC:Diadino, HPLC:Allo, HPLC:Diato, HPLC:Zea, HPLC:Lut, HPLC:Chl-b, HPLC:DVChl-a, HPLC:Chl-a, HPLC:Phe-a, HPLC:B-Car, HPLC:TChl-a, Flag:HPLC Primary file : D:\Telework\HPLC\2015-021\Processing\2015-021-0010.CHE2 Secondary file: D:\Telework\HPLC\2015-021\Processing\2015-021-0010.hplc -SORT parameters: 2020/07/21 14:05:55 Sorted in ascending order of channel Pressure -HEADER EDITS: 2020/07/21 14:06:08 Applied edit header: D:\Telework\HPLC\2015-021\Processing\DOC\2015-021-bot-hdr1.txt Channel 12: Number_of_bin_records [n/a] Pad: ' ' ==> -99 -HEADER EDITS: 2020/07/21 14:09:35 Applied edit header: D:\Telework\HPLC\2015-021\Processing\DOC\2015-021-bot-hdr2.txt -CLEAN functions: 2025/05/29 09:22:36 20 Change character data from " " to "0" in channels Flag* $END *COMMENTS SBE HEADER Sea-Bird SBE 9 Data File: FileName = C:\CTD Data\2015-21\2015-21-0010.hex Software Version Seasave V 7.23.2 Temperature SN = 2106 Conductivity SN = 2280 Number of Bytes Per Scan = 37 Number of Voltage Words = 4 Number of Scans Averaged by the Deck Unit = 1 System UpLoad Time = Sep 04 2015 18:58:54 NMEA Latitude = 48 32.21 N NMEA Longitude = 125 15.56 W NMEA UTC (Time) = Sep 04 2015 18:54:43 Store Lat/Lon Data = Append to Every Scan SBE 11plus V 5.1e number of scans to average = 1 pressure baud rate = 9600 NMEA baud rate = 4800 GPIB address = 1 advance primary conductivity 0.073 seconds advance secondary conductivity 0.073 seconds S> Mission: 2015-21 Ship: J.P. Tully Station: LB06 Depth: 113 System UTC = Sep 04 2015 18:58:54 # nquan = 21 # nvalues = 1928 # units = specified # name 0 = scan: Scan Count # name 1 = bpos: Bottle Position in Carousel # name 2 = nbf: Bottles Fired # name 3 = prDM: Pressure, Digiquartz [db] # name 4 = t090C: Temperature [ITS-90, deg C] # name 5 = t190C: Temperature, 2 [ITS-90, deg C] # name 6 = c0S/m: Conductivity [S/m] # name 7 = c1S/m: Conductivity, 2 [S/m] # name 8 = CStarTr0: Beam Transmission, WET Labs C-Star [%] # name 9 = flSP: Fluorescence, Seapoint # name 10 = sbeox0V: Oxygen raw, SBE 43 [V] # name 11 = par: PAR/Irradiance, Biospherical/Licor # name 12 = altM: Altimeter [m] # name 13 = dz/dtM: Descent Rate [m/s] # name 14 = pumps: Pump Status # name 15 = v6: Voltage 6 # name 16 = v7: Voltage 7 # name 17 = sal00: Salinity, Practical [PSU] # name 18 = sal11: Salinity, Practical, 2 [PSU] # name 19 = sbeox0ML/L: Oxygen, SBE 43 [ml/l] # name 20 = flag: 0.000e+00 # interval = seconds: 0.0416667 # start_time = Sep 04 2015 18:54:43 [NMEA time, header] # bad_flag = -9.990e-29 # # # # # 2106 # 17-Dec-14 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.14703785e-003 # 6.31044927e-004 # 2.13121431e-005 # 2.18594910e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 2280 # 19-Dec-14 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.00904009e+001 # 1.35149315e+000 # -1.84720682e-003 # 1.90856808e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 0443 # 17-Dec-14 # -4.435292e+004 # -1.635794e-001 # 1.192740e-002 # 3.979900e-002 # 0.000000e+000 # 2.993627e+001 # -3.271008e-004 # 3.759160e-006 # 1.565300e-009 # 0.99991613 # 0.05243 # 0.000000e+000 # 1.282462e-002 # -8.599824e+000 # # # # # # 2663 # 15-Jan-15 # 1 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.000 # 4.36565531e-003 # 6.42337654e-004 # 2.40578991e-005 # 2.42263656e-006 # 1000.000 # 1.00000000 # 0.0000 # # # # # # 2754 # 19-Dec-14 # 1 # # 0.0000 # 2000.0000 # 0 # # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.00000000e+000 # 0.0 # -9.57000000e-008 # # # -1.05920237e+001 # 1.61356129e+000 # -1.94911294e-003 # 2.41671700e-004 # -9.57000000e-008 # 3.2500e-006 # # 0.00000000e+000 # # 1.00000000 # 0.00000 # # # # # # 0997 # 17-Jan-15 # 1 # # # 0.0000 # 0.0000e+000 # 0.0000 # 0.00e+000 # 0.0000 # 0.0 # # # # 4.0785e-001 # -0.6967 # -3.5082e-003 # 1.2934e-004 # -1.8411e-006 # 2.5826e+000 # 1.92634e-004 # -4.64803e-002 # 3.7500e-002 # 1.6200 #

-3.3000e-002

#

5.0000e+003

#

1.4500e+003

# # # # # # # 3640 # # # 2 # 0.000 # # # # # # 1396DR # Feb 5th 2014 # 19.0844 # -0.0191 # 0.250 # # # # # # # # # 4565 QSP200L4S # 16-Mar-2011 # 1.00000000 # 0.00000000 # 4115226337.44999980 # 1.00000000 # -0.36780000 # # # # # # 62354 # # 15.000 # 0.000 # # # # # # # # # # datcnv_date = Jan 11 2016 11:46:05, 7.23.2 # datcnv_in = P:\Cruise_Data_Processing\2015-21\RAW\CTD data\2015-21-0010.hex P: \Cruise_Data_Processing\2015-21\Processing\doc\2015-21-ctd.XMLCON # datcnv_ox_hysteresis_correction = yes # datcnv_ox_tau_correction = yes # datcnv_bottle_scan_range_source = BL file # datcnv_scans_per_bottle = 241 END* Comments from secondary file: P:\Cruise_Data_Processing\2015-21\Processing\hydro\2015-21-0010.mrgcln1s ------------------------------------------------------------------------ Comments from secondary file: P:\Cruise_Data_Processing\2015-21\Processing\hydro\2015-21-0010.oxy ------------------------------------------------------------------- Sample_Number 22: OXY: Leaky spigot Sample_Number 23: OXY: Leaky spigot Sample_Number 26: OXY: There may have been a mix up with flask ID. Value seems reasonable but there is no way to verify due to missing electronic file Sample_Number 27: OXY: There may have been a mix up with flask ID. Value seems reasonable but there is no way to verify due to missing electronic file Comments from secondary file: P:\Cruise_Data_Processing\2015-21\Processing\hydro\2015-21-0010.NH4 ------------------------------------------------------------------- Sample_Number 25: NH4: large intercept and poor calibration curve, values likely underestimated Sample_Number 26: NH4: large intercept and poor calibration curve, values likely underestimated Sample_Number 27: NH4: large intercept and poor calibration curve, values likely underestimated Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in Room 2423a bout 2 weeks after the cruise. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “CV%” in file QF2015-21CHL*.xls. Precision Statement: Chlorophyll values ranged from 0.17-9.75 ug/l. Average %CV for this cruise was a very good 3.51% with 2 out of 104 duplicate pairs having a CV>15% and 0 out of 104 duplicate pairs having a CV>50% . Our average dataset %CV is 4 % for 2013 - 2014 so the overall quality of this dataset is very good. Flags and comments apply to chlorophyll values only. No flags or comments are assigned for Phaeopigment values. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6 weeks after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF2015-021HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2015-21OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with disposable plastic inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 76 to 82 days after collection. For details including a duplicate analysis, see document QF2015-21SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For details including a duplicate analysis, see document QF2015-21nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. For full details including a precision study see file 2015-21NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, Bethanie A., and Peterson, Bruce J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- CTD Data Processing Notes: -------------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". Dissolved oxygen was calibrated using the method described in SeaBird Application Note #64-2, June 2012 revision, except that a small offset in the fit was allowed. For details on the processing see the report: 2015-21_Processing_Report.doc. --------------------------------------------------------------------------------- *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.309E-01 1.0225) $END !-1-- 2- 3- --4--- ---5---- --6-- ---7--- --8--- ---9---- --10-- --11- -12- --13- ---14-- --15- 16 --17-- 18 --19-- 20 ---21-- 22 --23-- 24 --25-- 26 --27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- 53 !Samp Bo Bo Pressu Temperat Trans Fluores PAR Salinity Oxygen Oxyge Numb Tempe Oxygen: Oxyge Fl Nitrat Fl Silica Fl Phospha Fl Ammoni Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re ure: missi cence: :T1:C1 : n: er_o ratur Dissolv n: ag e_ ag te ag te ag um ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe-a Car TChl-a ag !Numb ~u ~u Secondar vity URU: Dissol Disso ~bin e: ed Disso ~o plus_ ~t ~i ~p ~o Extrac ~a t: a chlide a fuco fuco : !er mb en y Seapoin ved: lved: _rec Draw lved lv Nitrit ri ca ha ni ted ct Extrac HP ! er ce t SBE SBE ords ed e te te te um ed ted LC !---- -- -- ------ -------- ----- ------- ------ -------- ------ ----- ---- ----- ------- ----- -- ------ -- ------ -- ------- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 27 8 8 4.6 13.9676 46.5 0.886 1036.6 32.0324 6.36 277.3 241 14.6 6.336 276.3 2 2.26 0 4.37 0 0.548 0 0.09 36 2.40 6 0.43 0.137 0.068 0.021 0.219 0.033 0.054 0.114 0.000 0.003 0.676 0.041 0.037 0.024 0.028 0.328 0.116 0.044 0.039 0.021 0.147 0.000 1.914 0.091 0.042 2.036 6 26 7 7 10.0 13.8152 43.7 2.203 344.5 32.0490 6.37 277.6 241 14.3 6.324 275.7 2 2.47 0 4.55 0 0.551 0 0.29 36 1.92 6 0.49 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 25 6 6 19.9 13.3546 54.1 1.786 57.0 32.1547 5.69 247.9 241 12.9 5.341 232.8 0 8.70 0 11.88 0 1.024 0 1.34 36 1.66 6 0.71 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 24 5 5 30.3 9.4855 57.8 0.964 14.6 32.9280 3.87 168.6 241 10.0 3.919 170.6 0 21.15 0 26.85 0 1.794 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 23 4 4 49.8 8.0653 60.8 0.239 2.7 33.2492 3.70 161.2 241 8.8 3.681 160.2 3 24.98 0 31.44 0 1.927 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 22 3 3 75.5 7.8428 58.9 0.194 0.4 33.4697 3.01 131.1 241 8.6 3.022 131.5 3 25.69 6 40.74 6 2.153 6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 21 2 2 100.2 7.7584 54.2 0.175 0.0 33.5510 2.60 113.2 241 8.5 2.562 111.5 6 27.81 0 46.20 0 2.301 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 20 1 1 106.5 7.7085 49.6 0.161 0.0 33.5798 2.48 107.7 241 8.5 2.476 107.7 0 28.24 0 47.38 0 2.326 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0