*2021/11/22 12:53:03.08 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2013/06/15 16:41:12.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 10 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : D4BF8285 NUMBER OF CHANNELS : 46 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 494 503 2 Bottle_Number n/a 1 10 3 Bottle:Firing_Sequence n/a 1 10 4 Pressure decibar 5 60.6 5 Temperature:Primary 'deg C (ITS90)' 6.0063 9.2163 6 Transmissivity %/metre 60 62.4 7 Fluorescence:URU:Seapoint mg/m^3 1.379 1.616 8 Salinity:T0:C0 PSS-78 32.5266 32.6172 9 Oxygen:Dissolved:SBE mL/L 6.73 7.17 10 Oxygen:Dissolved:SBE umol/kg 293.1 312.3 11 Number_of_bin_records n/a 121 121 12 Nitrate_plus_Nitrite umol/L 11.6 15 13 Flag:Nitrate_plus_Nitrite n/a 14 Silicate umol/L 20 22.8 15 Flag:Silicate n/a 16 Phosphate umol/L 1.14 1.4 17 Flag:Phosphate n/a 18 Chlorophyll:Extracted mg/m^3 0.14 0.24 19 Flag:Chlorophyll:Extracted n/a 20 Phaeo-Pigment:Extracted mg/m^3 0.6E-01 0.13 21 HPLC:Chl-c3 mg/m^3 0.23E-01 0.44E-01 22 HPLC:Chlide-a mg/m^3 0 0 23 HPLC:MgDVP mg/m^3 0 0.6E-02 24 HPLC:Chl-c2 mg/m^3 0.25E-01 0.4E-01 25 HPLC:Chl-c1 mg/m^3 0 0 26 HPLC:Me-chlide mg/m^3 0 0 27 HPLC:Peri mg/m^3 0 0 28 HPLC:Pheide-a mg/m^3 0 0 29 HPLC:But-fuco mg/m^3 0.18E-01 0.35E-01 30 HPLC:Fuco mg/m^3 0.22E-01 0.38E-01 31 HPLC:Neo mg/m^3 0 0.3E-02 32 HPLC:Pras mg/m^3 0 0 33 HPLC:Viola mg/m^3 0 0.4E-02 34 HPLC:Hex-fuco mg/m^3 0.79E-01 0.121 35 HPLC:Diadino mg/m^3 0.2E-02 0.27E-01 36 HPLC:Allo mg/m^3 0 0 37 HPLC:Diato mg/m^3 0 0.5E-02 38 HPLC:Zea mg/m^3 0.7E-02 0.27E-01 39 HPLC:Lut mg/m^3 0 0.2E-02 40 HPLC:Chl-b mg/m^3 0.1E-01 0.34E-01 41 HPLC:DVChl-a mg/m^3 0 0 42 HPLC:Chl-a mg/m^3 0.164 0.226 43 HPLC:Phe mg/m^3 0 0 44 HPLC:B-Car mg/m^3 0 0.3E-02 45 HPLC:TChl-a mg/m^3 0.164 0.226 46 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 9 F ' ' 4 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 5 I I 0 12 -99 ' ' 6 F R4 1 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 6 F R4 1 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: ---------------------------------------------------------------------------------- 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable Measurement 3 = Questionable Measurement (Probably Good) 4 = Poor Measurement (Probably Bad) 5 = Measurement Not Reported (Bad) 6 = Mean of replicate measurements 7 = Manual chromatographic peak measurement 8 = Irregular digital chromatographic peak integration 9 = Sample was planned for this measurement from this bottle but was not collected ---------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2013-17 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P24 EVENT NUMBER : 75 LATITUDE : 49 50.22000 N ! (deg min) LONGITUDE : 142 40.04000 W ! (deg min) WATER DEPTH : 3086 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------------------------- -------------- ---------- Temperature ' ' 2023 Conductivity ' ' 2280 'Pressure:Digiquartz with TC' ' ' 0443 Temperature:2 ' ' 2668 Conductivity:2 ' ' 2754 Fluorometer:Seapoint ' ' 2228 'Oxygen:SBE 43' ' ' 0997 'Transmissometer:WET Labs C-Star' ' ' 1396 Altimeter ' ' 1204 PAR/Irradiance:Biospherical/Licor ' ' 4601 $END $REMARKS SOFTWARE VERSION SEASAVE V 7.22.4 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------ ------ ---------- -------- --------- --------- SBE_IOS 3.2 2013/08/30 14:30:18 1210 1210 CLEAN 5.1 2013/08/30 14:30:55 1210 1210 ADDSAMP 3.5 2013/11/07 15:38:59 1210 1210 BINAVE 4.1.1 2013/12/10 08:55:02 1210 10 MERGE 3.5 2013/12/10 08:55:29 10 10 CLEAN 5.1 2013/12/10 08:55:37 10 10 CALIB 11.8 2013/12/10 08:56:25 10 10 SORT 3.6 2013/12/10 08:57:41 10 10 REMOVECH 8.2 2013/12/10 08:58:31 10 10 CHGUNITS 3.1 2013/12/10 09:02:54 10 10 CHGUNITS 3.1 2013/12/10 09:03:21 10 10 REORDER 1.3 2013/12/10 09:03:53 ? ? HDREDIT2 2.6 2013/12/17 14:42:39 ? ? CHANGE_FLAGS 2.0 2014/11/20 09:58:29 10 10 HDREDIT2 3.0.2 2016/07/14 15:09:20 ? ? SORT 3.6 2017/01/18 17:06:47 10 10 REMOVECH 8.2 2017/01/18 17:19:31 10 10 HDREDIT2 3.0.2 2017/01/19 11:16:00 ? ? MERGE 3.5 2017/01/19 11:19:09 10 10 SORT 3.6 2017/01/19 11:19:14 10 10 REORDER 1.3.1 2017/01/19 11:19:23 ? ? HDREDIT2 3.0.2 2017/01/19 14:19:00 ? ? CLEAN 5.2.1 2017/01/19 14:19:05 10 10 SORT 3.6 2020/08/26 20:57:56 10 10 REORDER 1.3.1 2021/11/22 12:47:16 ? ? MERGE 3.5.1 2021/11/22 12:48:47 10 10 SORT 3.6 2021/11/22 12:50:05 10 10 CLEAN 5.2.4 2021/11/22 12:50:22 10 10 HDREDIT2 3.2 2021/11/22 12:50:58 ? ? HDREDIT2 3.2 2021/11/22 12:53:03 ? ? $END $REMARKS -CLEAN functions: 2013/08/30 14:30:34 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2013-17\Processing\HYDRO\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2013/12/10 08:55:29 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Event_Number, Bottle:Position, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2013-17\Processing\hydro\2013-17-0075.samavg Secondary file: P:\Cruise_Data_Processing\2013-17\Processing\hydro\2013-17-0075.mrgcln1s -SORT parameters: 2013/12/04 09:00:56 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2013/12/10 08:55:36 20 Reset #RECS, MIN & MAX values in header. Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2013/12/10 08:56:25 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2013-17\Processing\doc\2013-17-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 17 Oxygen:Dissolved:SBE mL/L 10 0.0000000E+00 0.1054000E+01 -SORT parameters: 2013/12/10 08:57:41 Sorted in ascending order of channel Pressure* -REMOVECH 2013/12/10 08:58:31 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: Temperature reference channel: Temperature:Primary Salinity reference channel: Salinity:T0:C0 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: Temperature reference channel: Temperature:Draw Salinity reference channel: Salinity:T0:C0 - No units changed -HEADER EDITS: 2013/12/17 14:42:39 Applied edit header: P:\Cruise_Data_Processing\2013-17\Processing\doc\HYDRO\2013-17-bot-hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 8: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 12: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 13: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 14: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 15: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Channel 16: Flag:Nitrate_plus_Nitrite [n/a] Units: ==> n/a Channel 17: Silicate [umol/L] Units: ==> umol/L Channel 18: Flag:Silicate [n/a] Units: ==> n/a Channel 19: Phosphate [umol/L] Units: ==> umol/L Channel 20: Flag:Phosphate [n/a] Units: ==> n/a -HEADER EDITS: 2016/07/14 15:09:20 Applied edit header: P:\Cruise_Data_Processing\2013-FL\2013-HDR-FL.TXT -SORT parameters: 2017/01/18 17:06:47 Sorted in ascending order of channel Sample_Number -REMOVECH 2017/01/18 17:19:31 The following CHANNEL(S) were removed: Chlorophyll:Extracted [mg/m^3] Flag:Chlorophyll:Extracted Phaeo-Pigment:Extracted [mg/m^3] -HEADER EDITS: 2017/01/19 11:16:00 -The following MERGE parameters were used: 2017/01/19 11:19:09 Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted Primary file : P:\Cruise_Data_Processing\2013-17\update_working_files\2013-17-0075.hdr1 Secondary file: P:\Cruise_Data_Processing\2013-17\update_working_files\2013-17-0075.chl -SORT parameters: 2017/01/19 11:19:14 Sorted in ascending order of channel Pressure -HEADER EDITS: 2017/01/19 14:19:00 Applied edit header: P:\Cruise_Data_Processing\Temp\2013-17-temp\update_working_files\2013-17-bot- hdr-new.txt Channel 12: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 13: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 14: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 -CLEAN functions: 2017/01/19 14:19:04 20 Change character data from " " to "0" in channels Flag* -SORT parameters: 2020/08/26 20:57:56 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/22 12:48:47 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2013\2013-017_Nov2021\Processing\ ios\2013-017-0075.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2013\2013-017_Nov2021\Processing\ ios\2013-017-0075.hplc -SORT parameters: 2021/11/22 12:50:05 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/22 12:50:21 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/22 12:50:58 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2013\2013-017\2013-017-bot- hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 21: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/22 12:53:03 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2013\2013-017\2013-017-bot- hdr2.txt $END *COMMENTS Comments from secondary file: P:\Cruise_Data_Processing\2013-17\update_working_files\2013-17-0075.chl ----------------------------------------------------------------------- Sample_Number 494: CHL: analysis on different instrument Sample_Number 495: CHL: analysis on different instrument Sample_Number 496: CHL: analysis on different instrument Sample_Number 497: CHL: analysis on different instrument Sample_Number 498: CHL: bad value, contamination suspected Sample_Number 500: CHL: bad value, contamination suspected Sample_Number 501: CHL: analysis on different instrument Sample_Number 502: CHL: analysis on different instrument Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in June or in July-August at IOS in a controlled temperature lab. A number of samples were analyzed on a different fluorometer (plankton lab) and values seem to be slightly lower than those analyzed on WP fluorometer. These are flagged. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “CV%” in file QF2013-17CHL*.xlsx where * with the most recent date is the most recent update. Flags and comments apply to chlorophyll values only. No flags or comments are assigned for Phaeopigment values. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6-8 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2013-017_HPLC*.xlsx. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2013-17OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 22 to 40 days after collection. Full details including a duplicate analysis are in file QF2013-17SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples was collected and frozen immediately. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For raw data, precision and duplicate details see file QF2013-17nuts*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers --------------------------------------------------------------------------------- Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". The CTD salinity was lower than bottle salinity by an average of ~0.0017. The Dissolved:Oxygen:SBE data are considered of lower quality than usual which is likely due to noisy secondary temperature and/or problems in the secondary plumbing. Problems were less severe during bottle stops except for casts #18, 19 and 22 from which the SBE DO channel was removed. For details on the processing see the report: 2013-17-proc.doc. --------------------------------------------------------------------------------- WARNING: SeaPoint Fluorometer #2228 malfunctioned during the latter half of 2013. There were large values at great depth that got steadily higher through the year until it was removed from service. The data have been left in the file so researchers can see the shape of the profile. Comparison with extracted chlorophyll is recommended. Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2013\2013-017_Nov2021\Processing\ios\2013-017-0075. hplc --------------------------------------------------------------------------- Sample_Number 497: HPLC: >10%CV for TChl-a Sample_Number 502: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0 1.054) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8---- --9--- --10- -11- --12- 13 --14- 15 --16-- 17 --18-- 19 --20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- 46 !Samp Bott Bott Pressu Temperat Trans Fluores Salinity Oxygen Oxyge Numb Nitra Fl Silic Fl Phosph Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: :T0:C0 : n: er_o te_ ag ate ag ate ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Primary vity URU: Dissol Disso ~bin plus_ ~t ~i ~p Extrac ~a t: a chlide a fuco fuco : !er er Sequ Seapoin ved: lved: _rec Nitri ri ca ha ted ct Extrac HP ! ence t SBE SBE ords te te te te ed ted LC !---- ---- ---- ------ -------- ----- ------- -------- ------ ----- ---- ----- -- ----- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 503 10 10 5.0 9.2160 60.2 1.379 32.5283 6.74 293.6 121 11.7 0 20.0 0 1.17 0 0.24 6 0.08 0.025 0.000 0.001 0.028 0.000 0.000 0.000 0.000 0.020 0.024 0.000 0.000 0.002 0.079 0.025 0.000 0.000 0.013 0.000 0.016 0.000 0.172 0.000 0.002 0.172 6 502 9 9 14.7 9.2163 60.2 1.426 32.5284 6.74 293.3 121 11.6 0 20.0 0 1.17 0 0.18 2 0.06 0.026 0.000 0.001 0.029 0.000 0.000 0.000 0.000 0.020 0.022 0.000 0.000 0.000 0.081 0.027 0.000 0.000 0.014 0.000 0.010 0.000 0.179 0.000 0.000 0.179 36 501 8 8 20.1 9.2093 60.0 1.446 32.5286 6.73 293.1 121 11.7 0 20.0 0 1.15 0 0.14 26 0.07 0.023 0.000 0.000 0.027 0.000 0.000 0.000 0.000 0.021 0.022 0.000 0.000 0.002 0.079 0.025 0.000 0.000 0.013 0.000 0.010 0.000 0.167 0.000 0.000 0.167 6 500 7 7 25.2 9.2162 60.2 1.456 32.5279 6.73 293.1 121 11.6 0 20.0 0 1.14 0 -99.00 5 -99.00 0.023 0.000 0.000 0.025 0.000 0.000 0.000 0.000 0.018 0.023 0.000 0.000 0.000 0.079 0.024 0.000 0.000 0.013 0.000 0.011 0.000 0.165 0.000 0.000 0.165 6 499 6 6 30.0 9.2081 60.3 1.437 32.5266 6.74 293.3 121 11.7 0 20.1 0 1.18 0 0.24 6 0.11 0.029 0.000 0.002 0.032 0.000 0.000 0.000 0.000 0.024 0.028 0.000 0.000 0.002 0.088 0.024 0.000 0.004 0.015 0.000 0.012 0.000 0.186 0.000 0.000 0.186 6 498 5 5 35.2 8.0693 60.1 1.574 32.5637 7.00 304.8 121 12.4 0 20.8 0 1.22 0 -99.00 5 -99.00 0.040 0.000 0.002 0.040 0.000 0.000 0.000 0.000 0.035 0.038 0.000 0.000 0.000 0.121 0.015 0.000 0.005 0.018 0.000 0.015 0.000 0.226 0.000 0.002 0.226 6 497 4 4 40.2 6.3311 61.5 1.616 32.6005 7.17 312.3 121 13.8 0 21.1 0 1.30 0 0.15 2 0.13 0.039 0.000 0.003 0.034 0.000 0.000 0.000 0.000 0.025 0.029 0.000 0.000 0.000 0.108 0.008 0.000 0.000 0.007 0.002 0.020 0.000 0.184 0.000 0.000 0.184 36 496 3 3 45.0 6.2745 61.6 1.608 32.6018 7.15 311.2 121 14.0 6 21.2 6 1.31 6 0.18 2 0.11 0.041 0.000 0.004 0.031 0.000 0.000 0.000 0.000 0.023 0.025 0.000 0.000 0.000 0.097 0.006 0.000 0.000 0.019 0.000 0.018 0.000 0.174 0.000 0.000 0.174 6 495 2 2 50.6 6.1909 61.9 1.553 32.6030 7.17 312.3 121 14.4 0 22.1 0 1.34 0 0.22 2 0.12 0.038 0.000 0.002 0.028 0.000 0.000 0.000 0.000 0.021 0.024 0.000 0.000 0.000 0.088 0.006 0.000 0.000 0.026 0.000 0.024 0.000 0.164 0.000 0.000 0.164 6 494 1 1 60.6 6.0063 62.4 1.501 32.6172 7.12 309.9 121 15.0 0 22.8 0 1.40 0 0.18 26 0.13 0.044 0.000 0.006 0.028 0.000 0.000 0.000 0.000 0.031 0.028 0.003 0.000 0.004 0.079 0.002 0.000 0.000 0.027 0.000 0.034 0.000 0.188 0.000 0.003 0.188 6