*2021/11/25 13:17:05.64 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2012/09/05 07:17:34.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 19 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 3BD654F3 NUMBER OF CHANNELS : 55 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 352 370 2 Bottle_Number n/a 1 19 3 Bottle:Firing_Sequence n/a 1 19 4 Pressure decibar 6.8 1284.3 5 Temperature:Secondary 'deg C (ITS90)' 2.8963 13.501 6 Transmissivity %/metre 52.2 66.1 7 Fluorescence:URU:Seapoint mg/m^3 0.31E-01 0.816 8 Fluorescence:URU:Wetlabs mg/m^3 0.86E-01 1.68 9 Salinity:T1:C1 PSS-78 31.7191 34.4603 10 Oxygen:Dissolved:SBE mL/L 0.28 6.06 11 Oxygen:Dissolved:SBE umol/kg 12.3 264.4 12 Number_of_bin_records n/a 121 121 13 Temperature:Draw 'deg C (ITS90)' 5.8 14.3 14 Salinity:Bottle PSS-78 34.0968 34.4597 15 Flag:Salinity:Bottle n/a 16 Oxygen:Dissolved mL/L 0.291 6.087 17 Oxygen:Dissolved umol/kg 12.7 265.5 18 Flag:Oxygen:Dissolved n/a 19 Nitrate_plus_Nitrite umol/L 4.8 45.1 20 Flag:Nitrate_plus_Nitrite n/a 21 Silicate umol/L 14.9 154.6 22 Flag:Silicate n/a 23 Phosphate umol/L 0.7 3.23 24 Flag:Phosphate n/a 25 Ammonium umol/L 0.75 2.74 26 Flag:Ammonium n/a 27 Chlorophyll:Extracted mg/m^3 0.41 0.47 28 Flag:Chlorophyll:Extracted n/a 29 Phaeo-Pigment:Extracted mg/m^3 0.69 0.7 30 HPLC:Chl-c3 mg/m^3 0.68E-01 0.68E-01 31 HPLC:Chlide-a mg/m^3 0.8E-02 0.8E-02 32 HPLC:MgDVP mg/m^3 0.21E-01 0.21E-01 33 HPLC:Chl-c2 mg/m^3 0.177 0.177 34 HPLC:Chl-c1 mg/m^3 0.6E-02 0.6E-02 35 HPLC:Me-chlide mg/m^3 0.7E-02 0.7E-02 36 HPLC:Peri mg/m^3 0.264 0.264 37 HPLC:Pheide-a mg/m^3 0 0 38 HPLC:But-fuco mg/m^3 0.27E-01 0.27E-01 39 HPLC:Fuco mg/m^3 0.232 0.232 40 HPLC:Neo mg/m^3 0.17E-01 0.17E-01 41 HPLC:Pras mg/m^3 0.34E-01 0.34E-01 42 HPLC:Viola mg/m^3 0.18E-01 0.18E-01 43 HPLC:Hex-fuco mg/m^3 0.93E-01 0.93E-01 44 HPLC:Diadino mg/m^3 0.9E-01 0.9E-01 45 HPLC:Allo mg/m^3 0.51E-01 0.51E-01 46 HPLC:Diato mg/m^3 0.5E-02 0.5E-02 47 HPLC:Zea mg/m^3 0.41E-01 0.41E-01 48 HPLC:Lut mg/m^3 0.3E-02 0.3E-02 49 HPLC:Chl-b mg/m^3 0.156 0.156 50 HPLC:DVChl-a mg/m^3 0 0 51 HPLC:Chl-a mg/m^3 1.317 1.317 52 HPLC:Phe mg/m^3 0.52E-01 0.52E-01 53 HPLC:B-Car mg/m^3 0.26E-01 0.26E-01 54 HPLC:TChl-a mg/m^3 1.332 1.332 55 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 9 F R4 4 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 6 F ' ' 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 6 F R4 1 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 6 F R4 1 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 3 NQ C ' ' 29 -99 ' ' 7 F R4 2 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2012-59 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : LBP5 EVENT NUMBER : 86 LATITUDE : 50 0.00000 N ! (deg min) LONGITUDE : 128 0.22000 W ! (deg min) WATER DEPTH : 1280 ALTIMETER (M) : 9.495001 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.21B $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1.2 2013/02/11 13:46:59 2299 2299 CLEAN 5.1 2013/02/11 13:47:55 2299 2299 ADDSAMP 3.5 2013/02/11 16:23:18 2299 2299 BINAVE 4.1.1 2013/02/11 16:23:34 2299 19 MERGE 3.4 2013/02/12 11:42:23 19 19 CLEAN 5.1 2013/02/12 11:42:46 ? ? CALIB 11.7 2013/02/22 09:02:18 19 19 SORT 3.6 2013/02/22 15:47:46 19 19 REMOVECH 8.1.1 2013/02/22 15:48:56 19 19 CHGUNITS 3.0 2013/02/22 15:52:11 19 19 CHGUNITS 3.0 2013/02/22 15:52:52 19 19 REORDER 1.3 2013/02/22 15:53:58 ? ? HDREDIT2 2.6 2013/02/22 15:55:42 ? ? CLEAN 5.1 2013/05/27 13:27:28 19 19 CALIB 11.7 2013/11/21 09:46:31 19 19 HDREDIT2 2.6 2013/11/21 09:51:29 ? ? CHANGE_FLAGS 2.0 2014/11/20 09:58:19 19 19 CHANGE_CTD_CHANNEL_NAMES 1.0 2015/01/13 11:33:17 19 19 SORT 3.6 2020/08/21 20:55:13 19 19 REORDER 1.3.1 2021/11/25 13:15:56 ? ? MERGE 3.5.1 2021/11/25 13:16:05 19 19 SORT 3.6 2021/11/25 13:16:14 19 19 CLEAN 5.2.4 2021/11/25 13:16:23 19 19 HDREDIT2 3.2 2021/11/25 13:16:46 ? ? HDREDIT2 3.2 2021/11/25 13:17:05 ? ? $END $REMARKS -CLEAN functions: 2013/02/11 13:47:39 20 Interpolation by Channel 1: Scan_Number [n/a] in Pressure Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2012-59\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2013/02/12 11:42:21 2013/02/12 11:42:21 2013/02/12 11:42: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Event_Number, Bottle:Position, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2012-59\Processing\hydro\2012-59-0086.samavg Secondary file: P:\Cruise_Data_Processing\2012-59\Processing\hydro\2012-59-0086.mrgcln1s -CLEAN functions: 2013/02/12 11:42:07 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2013/02/12 11:42:11 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2013/02/12 11:42:45 20 Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2013/02/22 09:02:18 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2012-59\Processing\doc\2012-59-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Oxygen:Dissolved:SBE mL/L 10 0.3290000E-01 0.1046500E+01 -SORT parameters: 2013/02/22 15:47:46 Sorted in ascending order of channel Pressure -REMOVECH 2013/02/22 15:48:56 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2013/02/22 15:55:42 Applied edit header: P:\Cruise_Data_Processing\2012-59\Processing\doc\HYDRO\2012-59-bot-hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 7: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:Wetlabs:ECO-AFL ==> Fluorescence:URU:Wetlabs Channel 8: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 Channel 9: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 13: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Format: F7.2 ==> F5.1 Channel 14: Salinity:Bottle [PSS-78] Units: ==> PSS-78 Channel 15: Flag:Salinity:Bottle [n/a] Units: ==> n/a Channel 16: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 17: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 18: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 19: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 21: Flag:Oxygen:Dissolved [n/a] Units: ==> n/a Channel 22: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Channel 23: Flag:Nitrate_plus_Nitrite [n/a] Units: ==> n/a Channel 24: Silicate [umol/L] Units: ==> umol/L Channel 25: Flag:Silicate [n/a] Units: ==> n/a Channel 26: Phosphate [umol/L] Units: ==> umol/L Channel 27: Flag:Phosphate [n/a] Units: ==> n/a Channel 29: Flag:Ammonium [n/a] Units: ==> n/a Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint -CLEAN functions: 2013/05/27 13:27:27 20 Reset #RECS, MIN & MAX values in header. -CALIB parameters: 2013/11/21 09:46:31 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 22 Nitrate_plus_Nitrite umol/L 10 0.1709000E+00 0.9812000E+00 26 Phosphate umol/L 10 0.4530000E-01 0.1011500E+01 -HEADER EDITS: 2013/11/21 09:51:29 - none. -SORT parameters: 2020/08/21 20:55:13 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 13:16:05 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2021-059_Nov2021\Processing\ IOS\2012-059-0086.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2021-059_Nov2021\Processing\ IOS\2012-059-0086.hplc -SORT parameters: 2021/11/25 13:16:14 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 13:16:22 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 13:16:46 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-059\2012-059-bot- hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 13: Temperature:Draw [deg C (ITS90)] Format: F5.1 ==> F6.1 Channel 30: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 13:17:05 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-059\2012-059-bot- hdr2.txt $END *COMMENTS Sample_Number 368: CHL: analytical error Sample_Number 369: CHL: CV 15-50% Sample_Number 352: OXY: No second shake or water seal. Sample_Number 353: OXY: No second shake or water seal. Sample_Number 354: OXY: Few small bubbles (no water seal) Sample_Number 355: OXY: Few small bubbles (no water seal) Sample_Number 356: OXY: Few small bubbles (no water seal) Sample_Number 357: OXY: No second shake or water seal. Sample_Number 358: OXY: No second shake or water seal. Sample_Number 359: OXY: No second shake or water seal. Sample_Number 360: OXY: No second shake or water seal. Sample_Number 361: OXY: No second shake or water seal. Sample_Number 362: OXY: Few small bubbles (no water seal) Sample_Number 363: OXY: No second shake or water seal. Sample_Number 364: OXY: No second shake or water seal. Sample_Number 365: OXY: No second shake or water seal. Sample_Number 366: OXY: No second shake or water seal. Sample_Number 367: OXY: No second shake or water seal. Sample_Number 368: OXY: No second shake or water seal. Sample_Number 369: OXY: No second shake or water seal. Sample_Number 370: OXY: No second shake or water seal. Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “Precision” in file QF2012-59CHL*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~7 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2012-059_HPLC*.xlsx. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 765 and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2012-59OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 2 months after collection. Full details including a duplicate analysis are in file QF2012-59SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. THey were analyzed using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For raw data, precision and duplicate details see worksheet QF2012-59nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. For full details including a precision study see file 2012-59_NH4*.xls. References*: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, Bethanie A., and Peterson, Bruce J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2. For further processing details see the processing report 2012-12-proc.doc. *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- -------- ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.329E-01 1.0465) Nitrate_plus_Nitrite umol/L 10 -99 () (0.1709 0.9812) Phosphate umol/L 10 -99 () (0.453E-01 1.0115) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8--- ---9---- --10-- --11- -12- --13- ---14--- 15 ---16-- --17- 18 --19- 20 --21- 22 --23-- 24 --25-- 26 --27-- 28 --29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- 55 !Samp Bott Bott Pressu Temperat Trans Fluores Fluores Salinity Oxygen Oxyge Numb Tempe Salinity Fl Oxygen: Oxyge Fl Nitra Fl Silic Fl Phosph Fl Ammoni Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: cence: :T1:C1 : n: er_o ratur :Bottle ag Dissolv n: ag te_ ag ate ag ate ag um ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: URU: Dissol Disso ~bin e: ~o ed Disso ~o plus_ ~t ~i ~p ~o Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin Wetlabs ved: lved: _rec Draw tt lved lv Nitri ri ca ha ni ted ct Extrac HP ! ence t SBE SBE ords le ed te te te te um ed ted LC !---- ---- ---- ------ -------- ----- ------- ------- -------- ------ ----- ---- ----- -------- -- ------- ----- -- ----- -- ----- -- ------ -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 370 19 19 6.8 13.5010 52.2 0.805 1.676 31.7198 6.06 264.4 121 14.3 -99.0000 0 6.087 265.5 36 4.8 0 14.9 0 0.70 0 0.77 6 0.41 6 0.70 0.068 0.008 0.021 0.177 0.006 0.007 0.264 0.000 0.027 0.232 0.017 0.034 0.018 0.093 0.090 0.051 0.005 0.041 0.003 0.156 0.000 1.317 0.052 0.026 1.332 6 369 18 18 11.7 13.4923 52.4 0.816 1.680 31.7191 6.06 264.3 121 14.1 -99.0000 0 6.047 263.7 3 5.0 0 15.1 0 0.73 0 0.75 6 0.47 36 0.69 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 368 17 17 22.1 10.8102 61.3 0.199 0.435 32.0049 4.52 196.9 121 11.5 -99.0000 0 4.547 198.2 3 15.1 0 27.1 0 1.41 0 2.74 6 -99.00 5 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 367 16 16 31.2 8.5312 63.9 0.103 0.236 32.6039 4.69 204.3 121 9.5 -99.0000 0 4.438 193.3 3 19.6 0 29.5 0 1.70 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 366 15 15 51.9 7.4609 66.0 0.032 0.100 33.3031 4.40 191.4 121 8.4 -99.0000 0 4.435 193.0 3 22.2 0 27.7 0 1.72 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 365 14 14 75.4 7.6296 66.1 0.035 0.086 33.6378 3.37 146.6 121 8.6 -99.0000 0 3.401 148.0 3 27.3 0 33.7 0 1.89 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 364 13 13 101.0 7.2905 65.5 0.033 0.088 33.8137 2.93 127.3 121 8.3 -99.0000 0 2.933 127.6 3 30.1 6 40.0 6 2.17 6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 363 12 12 126.5 6.9727 65.2 0.033 0.098 33.8923 2.57 111.8 121 8.2 -99.0000 0 2.566 111.6 36 32.2 0 45.2 0 2.23 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 362 11 11 151.4 6.6782 63.5 0.035 0.106 33.9139 2.19 95.4 121 7.9 -99.0000 0 2.214 96.3 4 34.1 0 51.3 0 2.24 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 361 10 10 176.4 6.6104 63.5 0.034 0.104 33.9216 2.07 90.0 121 7.9 -99.0000 0 2.044 88.9 3 34.6 0 52.9 0 2.41 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 360 9 9 201.4 6.5110 64.5 0.034 0.097 33.9359 2.04 88.7 121 7.8 -99.0000 0 2.030 88.3 3 34.9 0 54.1 0 2.39 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 359 8 8 252.0 6.3258 64.9 0.033 0.102 33.9684 1.70 74.0 121 7.7 -99.0000 0 1.716 74.6 3 36.8 0 58.3 0 2.60 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 358 7 7 301.6 6.0506 65.7 0.031 0.105 34.0066 1.36 59.2 121 7.5 -99.0000 0 1.356 59.0 3 38.6 0 64.0 0 2.70 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 357 6 6 401.2 5.6994 65.3 0.036 0.109 34.0504 1.02 44.2 121 7.3 -99.0000 0 1.024 44.5 3 40.5 0 72.5 0 2.82 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 356 5 5 501.4 5.2772 64.8 0.036 0.108 34.0960 0.75 32.5 121 7.1 34.0968 0 0.757 32.9 4 42.0 6 82.2 6 3.01 6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 355 4 4 751.5 4.3203 66.1 0.037 0.122 34.2622 0.28 12.3 121 6.6 34.2618 0 0.291 12.7 46 44.4 0 109.8 0 3.14 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 354 3 3 1002.3 3.4944 63.3 0.038 0.123 34.3695 0.32 14.0 121 6.1 34.3754 6 0.302 13.1 4 44.9 0 136.1 0 3.21 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 353 2 2 1252.0 2.9316 61.9 0.035 0.119 34.4546 0.47 20.6 121 5.8 34.4597 6 0.460 20.0 3 45.1 0 152.9 0 3.23 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 352 1 1 1284.3 2.8963 60.9 0.037 0.118 34.4603 0.49 21.1 121 5.9 -99.0000 0 0.484 21.0 3 44.9 0 154.6 0 3.22 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0