*2021/11/25 13:17:04.04 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2012/09/02 22:35:52.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 18 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 5B1E494F NUMBER OF CHANNELS : 53 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 132 149 2 Bottle_Number n/a 1 18 3 Bottle:Firing_Sequence n/a 1 18 4 Pressure decibar 6.2 645.6 5 Temperature:Secondary 'deg C (ITS90)' 4.7806 14.0953 6 Transmissivity %/metre 16.9 66.7 7 Fluorescence:URU:Seapoint mg/m^3 0.24E-01 6.262 8 Fluorescence:URU:Wetlabs mg/m^3 0.107 11.113 9 Salinity:T1:C1 PSS-78 31.814 34.1957 10 Oxygen:Dissolved:SBE mL/L 0.4 6.82 11 Oxygen:Dissolved:SBE umol/kg 17.2 297.3 12 Number_of_bin_records n/a 118 121 13 Temperature:Draw 'deg C (ITS90)' 7.2 15 14 Oxygen:Dissolved mL/L 0.391 6.843 15 Oxygen:Dissolved umol/kg 17 298.5 16 Flag:Oxygen:Dissolved n/a 17 Nitrate_plus_Nitrite umol/L 0.2 43.7 18 Flag:Nitrate_plus_Nitrite n/a 19 Silicate umol/L 6.8 96 20 Flag:Silicate n/a 21 Phosphate umol/L 0.48 3.22 22 Flag:Phosphate n/a 23 Ammonium umol/L 0.19 0.73 24 Flag:Ammonium n/a 25 Chlorophyll:Extracted mg/m^3 0.18 0.18 26 Flag:Chlorophyll:Extracted n/a 27 Phaeo-Pigment:Extracted mg/m^3 0.89 0.89 28 HPLC:Chl-c3 mg/m^3 0.454 0.496 29 HPLC:Chlide-a mg/m^3 0.38E-01 0.41E-01 30 HPLC:MgDVP mg/m^3 0.52E-01 0.66E-01 31 HPLC:Chl-c2 mg/m^3 0.732 0.804 32 HPLC:Chl-c1 mg/m^3 0 0 33 HPLC:Me-chlide mg/m^3 0 0 34 HPLC:Peri mg/m^3 0.142 0.151 35 HPLC:Pheide-a mg/m^3 0 0 36 HPLC:But-fuco mg/m^3 0.233 0.253 37 HPLC:Fuco mg/m^3 1.801 2.159 38 HPLC:Neo mg/m^3 0.38E-01 0.61E-01 39 HPLC:Pras mg/m^3 0.59E-01 0.6E-01 40 HPLC:Viola mg/m^3 0.49E-01 0.53E-01 41 HPLC:Hex-fuco mg/m^3 0.691 0.717 42 HPLC:Diadino mg/m^3 0.277 0.361 43 HPLC:Allo mg/m^3 0.61E-01 0.62E-01 44 HPLC:Diato mg/m^3 0.33E-01 0.48E-01 45 HPLC:Zea mg/m^3 0.82E-01 0.89E-01 46 HPLC:Lut mg/m^3 0.5E-02 0.5E-02 47 HPLC:Chl-b mg/m^3 0.366 0.405 48 HPLC:DVChl-a mg/m^3 0 0 49 HPLC:Chl-a mg/m^3 3.755 4.162 50 HPLC:Phe mg/m^3 0 0 51 HPLC:B-Car mg/m^3 0.67E-01 0.74E-01 52 HPLC:TChl-a mg/m^3 3.793 4.203 53 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 8 F R4 3 15 -99 ' ' 6 F ' ' 1 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 6 F R4 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 6 F R4 1 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2012-59 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : LC09 EVENT NUMBER : 40 LATITUDE : 48 25.87000 N ! (deg min) LONGITUDE : 126 14.00000 W ! (deg min) WATER DEPTH : 645 ALTIMETER (M) : 11.6 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.21B $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1.2 2013/02/11 13:46:48 2178 2178 CLEAN 5.1 2013/02/11 13:47:45 2178 2178 CTDEDIT 4.61 2013/02/11 13:54:34 2178 2178 ADDSAMP 3.5 2013/02/11 16:23:09 2178 2178 BINAVE 4.1.1 2013/02/11 16:23:29 2178 18 MERGE 3.4 2013/02/12 11:42:22 18 18 CLEAN 5.1 2013/02/12 11:42:46 ? ? CALIB 11.7 2013/02/22 09:02:16 18 18 SORT 3.6 2013/02/22 15:47:45 18 18 REMOVECH 8.1.1 2013/02/22 15:48:56 18 18 CHGUNITS 3.0 2013/02/22 15:52:10 18 18 CHGUNITS 3.0 2013/02/22 15:52:52 18 18 REORDER 1.3 2013/02/22 15:53:57 ? ? HDREDIT2 2.6 2013/02/22 15:55:41 ? ? CLEAN 5.1 2013/05/27 13:27:27 18 18 CALIB 11.7 2013/11/21 09:46:30 18 18 HDREDIT2 2.6 2013/11/21 09:51:29 ? ? CHANGE_FLAGS 2.0 2014/11/20 09:58:18 18 18 CHANGE_CTD_CHANNEL_NAMES 1.0 2015/01/13 11:33:17 18 18 SORT 3.6 2020/08/21 20:55:13 18 18 REORDER 1.3.1 2021/11/25 13:15:55 ? ? MERGE 3.5.1 2021/11/25 13:16:04 18 18 SORT 3.6 2021/11/25 13:16:13 18 18 CLEAN 5.2.4 2021/11/25 13:16:22 18 18 HDREDIT2 3.2 2021/11/25 13:16:44 ? ? HDREDIT2 3.2 2021/11/25 13:17:04 ? ? $END $REMARKS -CLEAN functions: 2013/02/11 13:47:39 20 Interpolation by Channel 1: Scan_Number [n/a] in Pressure Set event to last 4 characters of file name -CTDEDIT 2013/02/11 13:54:34 3 changes in channel Salinity:T0:C0 -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2012-59\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2013/02/12 11:42:21 2013/02/12 11:42:21 2013/02/12 11:42: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Event_Number, Bottle:Position, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2012-59\Processing\hydro\2012-59-0040.samavg Secondary file: P:\Cruise_Data_Processing\2012-59\Processing\hydro\2012-59-0040.mrgcln1s -CLEAN functions: 2013/02/12 11:42:07 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2013/02/12 11:42:10 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2013/02/12 11:42:45 20 Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2013/02/22 09:02:16 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2012-59\Processing\doc\2012-59-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Oxygen:Dissolved:SBE mL/L 10 0.3290000E-01 0.1046500E+01 -SORT parameters: 2013/02/22 15:47:45 Sorted in ascending order of channel Pressure -REMOVECH 2013/02/22 15:48:56 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2013/02/22 15:55:41 Applied edit header: P:\Cruise_Data_Processing\2012-59\Processing\doc\HYDRO\2012-59-bot-hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 7: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:Wetlabs:ECO-AFL ==> Fluorescence:URU:Wetlabs Channel 8: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 Channel 9: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 13: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Format: F7.2 ==> F5.1 Channel 14: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 15: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 16: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 17: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 19: Flag:Oxygen:Dissolved [n/a] Units: ==> n/a Channel 20: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Channel 21: Flag:Nitrate_plus_Nitrite [n/a] Units: ==> n/a Channel 22: Silicate [umol/L] Units: ==> umol/L Channel 23: Flag:Silicate [n/a] Units: ==> n/a Channel 24: Phosphate [umol/L] Units: ==> umol/L Channel 25: Flag:Phosphate [n/a] Units: ==> n/a Channel 27: Flag:Ammonium [n/a] Units: ==> n/a Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint -CLEAN functions: 2013/05/27 13:27:27 20 Reset #RECS, MIN & MAX values in header. -CALIB parameters: 2013/11/21 09:46:30 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 20 Nitrate_plus_Nitrite umol/L 10 0.1709000E+00 0.9812000E+00 24 Phosphate umol/L 10 0.4530000E-01 0.1011500E+01 -HEADER EDITS: 2013/11/21 09:51:29 - none. -SORT parameters: 2020/08/21 20:55:13 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 13:16:04 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2021-059_Nov2021\Processing\ IOS\2012-059-0040.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2021-059_Nov2021\Processing\ IOS\2012-059-0040.hplc -SORT parameters: 2021/11/25 13:16:13 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 13:16:22 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 13:16:44 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-059\2012-059-bot- hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 13: Temperature:Draw [deg C (ITS90)] Format: F5.1 ==> F6.1 Channel 28: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 13:17:04 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-059\2012-059-bot- hdr2.txt $END *COMMENTS CTDEDIT was used to remove 3 points in primary salinity from the surface bottle. Sample_Number 145: CHL: Value low compared to SeaPoint fluorescence. Sample_Number 146: CHL: Lost sample in analysis Sample_Number 148: CHL: Value severe outlier compared to SeaPoint fluorescence and HPLC chl a. Sample_Number 149: CHL: Value severe outlier compared to SeaPoint fluorescence and HPLC chl a. Sample_Number 132: OXY: Niskin spigot leaking Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “Precision” in file QF2012-59CHL*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~7 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2012-059_HPLC*.xlsx. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 765 and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2012-59OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 2 months after collection. Full details including a duplicate analysis are in file QF2012-59SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. THey were analyzed using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For raw data, precision and duplicate details see worksheet QF2012-59nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. For full details including a precision study see file 2012-59_NH4*.xls. References*: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, Bethanie A., and Peterson, Bruce J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2. For further processing details see the processing report 2012-12-proc.doc. Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2021-059_Nov2021\Processing\IOS\2012-059-0040. hplc --------------------------------------------------------------------------- Sample_Number 149: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- -------- ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.329E-01 1.0465) Nitrate_plus_Nitrite umol/L 10 -99 () (0.1709 0.9812) Phosphate umol/L 10 -99 () (0.453E-01 1.0115) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8--- ---9---- --10-- --11- -12- --13- ---14-- --15- 16 --17- 18 --19- 20 --21-- 22 --23-- 24 --25-- 26 --27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- 53 !Samp Bott Bott Pressu Temperat Trans Fluores Fluores Salinity Oxygen Oxyge Numb Tempe Oxygen: Oxyge Fl Nitra Fl Silic Fl Phosph Fl Ammoni Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: cence: :T1:C1 : n: er_o ratur Dissolv n: ag te_ ag ate ag ate ag um ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: URU: Dissol Disso ~bin e: ed Disso ~o plus_ ~t ~i ~p ~o Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin Wetlabs ved: lved: _rec Draw lved lv Nitri ri ca ha ni ted ct Extrac HP ! ence t SBE SBE ords ed te te te te um ed ted LC !---- ---- ---- ------ -------- ----- ------- ------- -------- ------ ----- ---- ----- ------- ----- -- ----- -- ----- -- ------ -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 148 17 18 6.2 14.0947 16.9 5.259 9.221 31.8271 6.82 297.3 121 -99.0 -99.000 -99.0 0 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 5 -99.00 0.454 0.038 0.052 0.732 0.000 0.000 0.151 0.000 0.233 1.801 0.038 0.059 0.049 0.691 0.361 0.061 0.048 0.089 0.005 0.366 0.000 3.755 0.000 0.067 3.793 6 147 16 17 6.4 14.0953 17.0 5.283 9.239 31.8271 6.82 297.3 121 15.0 6.843 298.5 0 0.2 0 6.8 0 0.48 0 0.19 6 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 146 15 15 11.0 13.9512 18.5 6.262 10.883 31.8143 6.79 296.3 118 14.6 6.775 295.5 0 0.4 0 7.4 0 0.51 0 0.23 6 -99.00 5 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 149 18 16 11.0 13.9297 18.6 6.257 11.113 31.8140 6.79 296.1 121 -99.0 -99.000 -99.0 0 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 5 -99.00 0.496 0.041 0.066 0.804 0.000 0.000 0.142 0.000 0.253 2.159 0.061 0.060 0.053 0.717 0.277 0.062 0.033 0.082 0.005 0.405 0.000 4.162 0.000 0.074 4.203 36 145 14 14 21.1 9.5511 50.7 0.847 2.117 32.4765 6.00 261.5 121 10.3 6.034 262.8 0 9.0 0 12.1 0 1.11 0 0.73 6 0.18 36 0.89 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 144 13 13 31.4 8.4795 63.0 0.246 0.455 32.6370 6.05 263.2 121 9.3 6.049 263.4 6 10.9 0 13.2 0 1.00 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 143 12 12 51.4 7.4527 64.7 0.056 0.161 32.7230 6.21 270.3 121 8.4 6.128 266.8 0 13.8 0 17.7 0 1.28 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 142 11 11 76.3 7.9494 65.8 0.040 0.131 33.4483 3.71 161.4 121 8.9 3.731 162.4 0 24.5 0 28.8 0 1.89 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 141 10 10 101.6 7.6829 65.3 0.041 0.139 33.7330 3.01 130.9 121 8.7 3.007 130.8 0 29.0 6 35.7 6 2.12 6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 140 9 9 126.2 7.4008 64.8 0.037 0.138 33.8380 2.69 116.8 121 8.5 2.675 116.4 0 31.1 0 40.5 0 2.28 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 139 8 8 151.1 7.1116 64.7 0.030 0.119 33.8984 2.15 93.3 121 8.3 2.151 93.6 0 33.4 0 47.6 0 2.45 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 138 7 7 176.2 6.9147 63.8 0.026 0.117 33.9236 1.93 83.9 121 8.1 1.947 84.7 0 34.4 0 50.9 0 2.57 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 137 6 6 201.4 6.6947 62.2 0.032 0.127 33.9428 1.78 77.4 121 8.0 1.792 77.9 0 35.5 0 53.9 0 2.59 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 136 5 5 251.5 6.4690 64.7 0.027 0.114 33.9739 1.70 73.8 121 7.9 1.684 73.2 6 36.6 0 56.9 0 2.58 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 135 4 4 301.1 6.2769 65.4 0.024 0.120 33.9988 1.51 65.7 121 7.7 1.508 65.6 0 37.8 0 60.5 0 2.72 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 134 3 3 400.7 5.8937 66.1 0.026 0.109 34.0381 1.16 50.4 121 7.6 1.161 50.5 0 39.8 0 68.3 0 2.86 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 133 2 2 501.5 5.3849 66.7 0.024 0.107 34.0954 0.77 33.4 121 7.4 0.765 33.3 0 41.7 0 79.4 0 2.49 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 132 1 1 645.6 4.7806 66.2 0.024 0.115 34.1957 0.40 17.2 121 7.2 0.391 17.0 2 43.7 6 96.0 6 3.22 6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0