*2021/11/25 13:12:46.72 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2012/05/18 10:05:18.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 17 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 7A894BE7 NUMBER OF CHANNELS : 55 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 342 358 2 Bottle_Number n/a 1 17 3 Bottle:Firing_Sequence n/a 1 17 4 Pressure decibar 6.4 1401.3 5 Temperature:Secondary 'deg C (ITS90)' 2.6214 8.9681 6 Transmissivity %/metre 37.5 67.8 7 Fluorescence:URU:Seapoint mg/m^3 0 3.9 8 Fluorescence:URU:Wetlabs mg/m^3 0.14 6.418 9 Salinity:T1:C1 PSS-78 31.2617 34.4811 10 Oxygen:Dissolved:SBE mL/L 0.29 7.24 11 Oxygen:Dissolved:SBE umol/kg 12.8 315.8 12 Number_of_bin_records n/a 241 241 13 Temperature:Draw 'deg C (ITS90)' 3.8 8.9 14 Salinity:Bottle PSS-78 31.2654 34.2546 15 Flag:Salinity:Bottle n/a 16 Oxygen:Dissolved mL/L 0.283 7.234 17 Oxygen:Dissolved umol/kg 12.3 315.3 18 Flag:Oxygen:Dissolved n/a 19 Nitrate_plus_Nitrite umol/L 4.6 46.6 20 Flag:Nitrate_plus_Nitrite n/a 21 Silicate umol/L 11.6 156.6 22 Flag:Silicate n/a 23 Phosphate umol/L 0.65 3.24 24 Flag:Phosphate n/a 25 Ammonium umol/L 0.52 0.81 26 Flag:Ammonium n/a 27 Chlorophyll:Extracted mg/m^3 4.8 6.54 28 Flag:Chlorophyll:Extracted n/a 29 Phaeo-Pigment:Extracted mg/m^3 0.65 0.78 30 HPLC:Chl-c3 mg/m^3 0.339 0.339 31 HPLC:Chlide-a mg/m^3 0.107 0.107 32 HPLC:MgDVP mg/m^3 0.6E-01 0.6E-01 33 HPLC:Chl-c2 mg/m^3 0.846 0.846 34 HPLC:Chl-c1 mg/m^3 0.27E-01 0.27E-01 35 HPLC:Me-chlide mg/m^3 0.53E-01 0.53E-01 36 HPLC:Peri mg/m^3 0.72E-01 0.72E-01 37 HPLC:Pheide-a mg/m^3 0 0 38 HPLC:But-fuco mg/m^3 0.33E-01 0.33E-01 39 HPLC:Fuco mg/m^3 3.027 3.027 40 HPLC:Neo mg/m^3 0.21E-01 0.21E-01 41 HPLC:Pras mg/m^3 0.34E-01 0.34E-01 42 HPLC:Viola mg/m^3 0.18E-01 0.18E-01 43 HPLC:Hex-fuco mg/m^3 0.193 0.193 44 HPLC:Diadino mg/m^3 0.262 0.262 45 HPLC:Allo mg/m^3 0.122 0.122 46 HPLC:Diato mg/m^3 0.13E-01 0.13E-01 47 HPLC:Zea mg/m^3 0.22E-01 0.22E-01 48 HPLC:Lut mg/m^3 0 0 49 HPLC:Chl-b mg/m^3 0.167 0.167 50 HPLC:DVChl-a mg/m^3 0 0 51 HPLC:Chl-a mg/m^3 5.426 5.426 52 HPLC:Phe mg/m^3 0 0 53 HPLC:B-Car mg/m^3 0.79E-01 0.79E-01 54 HPLC:TChl-a mg/m^3 5.586 5.586 55 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 9 F R4 4 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 6 F ' ' 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 6 F R4 1 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 6 F R4 1 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 3 NQ C ' ' 29 -99 ' ' 7 F R4 2 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 8 F R4 3 54 -99 ' ' 8 F R4 3 55 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2012-25 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Yelland D. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : CS02 EVENT NUMBER : 89 LATITUDE : 50 41.24000 N ! (deg min) LONGITUDE : 129 28.12000 W ! (deg min) WATER DEPTH : 1894 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.21B $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1.2 2012/07/04 09:54:08 4097 4097 CLEAN 5.0.4 2012/07/04 10:10:13 4097 4097 ADDSAMP 3.5 2012/07/04 10:40:24 4097 4097 BINAVE 4.1.1 2012/07/04 10:41:09 4097 17 MERGE 3.4 2012/07/31 09:02:26 17 17 CLEAN 5.0.4 2012/07/31 09:02:37 17 17 CALIB 11.7 2012/08/03 12:02:54 17 17 SORT 3.5 2012/08/03 13:50:05 17 17 REMOVECH 8.1.1 2012/08/03 13:52:39 17 17 CHGUNITS 3.0 2012/08/03 13:56:02 17 17 CHGUNITS 3.0 2012/08/03 13:56:20 17 17 REORDER 1.3 2012/08/03 13:59:22 ? ? HDREDIT2 2.5.1 2012/08/06 14:20:09 ? ? CALIB 11.7 2013/06/04 13:18:35 17 17 HDREDIT2 2.6 2013/07/08 08:52:29 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:38:08 17 17 CHANGE_FLAGS 2.0 2014/11/20 09:58:14 17 17 SORT 3.6 2020/08/21 20:01:46 17 17 REORDER 1.3.1 2021/11/25 13:11:23 ? ? MERGE 3.5.1 2021/11/25 13:11:47 17 17 SORT 3.6 2021/11/25 13:11:56 17 17 CLEAN 5.2.4 2021/11/25 13:12:03 17 17 HDREDIT2 3.2 2021/11/25 13:12:26 ? ? HDREDIT2 3.2 2021/11/25 13:12:46 ? ? $END $REMARKS -CLEAN functions: 2012/07/04 10:09:43 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2012-25\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2012/07/31 09:02:23 2012/07/31 09:02:23 2012/07/31 09:02: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Event_Number, Bottle:Position, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2012-25\processing\hydro\2012-25-0089.samavg Secondary file: P:\Cruise_Data_Processing\2012-25\processing\hydro\2012-25-0089.mrgcln1s -CLEAN functions: 2012/07/31 09:02:11 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2012/07/31 09:02:16 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2012/07/31 09:02:36 20 Reset #RECS, MIN & MAX values in header. Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2012/08/03 12:02:54 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2012-25\Processing\doc\2012-25-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 17 Salinity:T0:C0 PSS-78 10 0.5000000E-02 0.1000000E+01 18 Salinity:T1:C1 PSS-78 10 0.1500000E-02 0.1000000E+01 -SORT parameters: 2012/08/03 13:50:05 Sorted in ascending order of channel Pressure [dbars] -REMOVECH 2012/08/03 13:52:39 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] PAR [uE/m^2/sec] Oxygen:Voltage:SBE [volts] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2012/08/06 14:20:09 Applied edit header: P:\Cruise_Data_Processing\2012-25\Processing\doc\2012-25-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 7: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:Wetlabs:ECO-AFL ==> Fluorescence:URU:Wetlabs Channel 8: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 Channel 9: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 13: Temperature:Draw [deg C (ITS90)] Units: ==> deg C (ITS90) Channel 14: Salinity:Bottle [PSS-78] Units: ==> PSS-78 Channel 15: Flag:Salinity:Bottle [n/a] Units: ==> n/a Channel 17: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 19: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 21: Flag:Oxygen:Dissolved [n/a] Units: ==> n/a Channel 22: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Channel 23: Flag:Nitrate_plus_Nitrite [n/a] Units: ==> n/a Channel 24: Silicate [umol/L] Units: ==> umol/L Format: F7.2 ==> F6.1 Channel 25: Flag:Silicate [n/a] Units: ==> n/a Channel 26: Phosphate [umol/L] Units: ==> umol/L Channel 27: Flag:Phosphate [n/a] Units: ==> n/a Channel 28: Ammonium [umol/L] Format: F8.4 ==> F7.2 Channel 29: Flag:Ammonium [n/a] Units: ==> n/a Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Format: F5.1 ==> F8.3 -CALIB parameters: 2013/06/04 13:18:35 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 22 Nitrate_plus_Nitrite umol/L 10 0.1709000E+00 0.9812000E+00 26 Phosphate umol/L 10 0.4530000E-01 0.1011500E+01 -HEADER EDITS: 2013/07/08 08:52:29 Applied edit header: C:\DATA\Nutrients\doc\headedit-correction.txt -SORT parameters: 2020/08/21 20:01:46 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 13:11:47 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025_Nov2021\Processing\ IOS\2012-025-0089.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025_Nov2021\Processing\ IOS\2012-025-0089.hplc -SORT parameters: 2021/11/25 13:11:56 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 13:12:02 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 13:12:26 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025\2012-025-bot- hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 13: Temperature:Draw [deg C (ITS90)] Format: F5.1 ==> F6.1 Channel 30: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 53: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 54: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 13:12:46 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025\2012-025-bot- hdr2.txt $END *COMMENTS Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone for 24 hours at -20C within 10 days of collection and analyzed on a Turner 10AU fluorometer calibrated annually with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “CV%” in file QF2012-25_CHL-*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2012-025_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Brinkmann Dosimat model 765 and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2012-25oxy*.xls. Salinity samples were collected in 200 mL type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and crew caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 1 month after collection. Full details are in file QF2012-25SAL*.xls. Nutrient samples were collected in plastic tubes. One set of samples was collected and and quick frozen in aluminum blocks stored in -20C freezer. These samples were returned to IOS for analysis. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. Theses samples were analyzed at sea during cruise 2012-12 (Line P). All samples were analyzed using the Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For precision and duplicate study see file QF2012-25nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. For full details including a precision study see file 2012-25_NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, Bethanie A., and Peterson, Bruce J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2. For further processing details see the processing report 2012-25-proc.doc. July 2013: A correction has been applied to Nitrate_plus_Nitrite and Phosphate data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the following report: Corrections to Nitrate and Phosphate for Deep-Water Cruises from 2009 to 2012.pdf which is stored in folder: \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025_Nov2021\Processing\IOS\2012-025-0089. hplc --------------------------------------------------------------------------- Sample_Number 358: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- -------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.5E-02 1) Salinity:T1:C1 PSS-78 10 -99 () (0.15E-02 1) Nitrate_plus_Nitrite umol/L 10 -99 () (0.1709 0.9812) Phosphate umol/L 10 -99 () (0.453E-01 1.0115) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8--- ---9---- --10-- --11- -12- --13- ---14--- 15 ---16-- --17- 18 --19- 20 --21- 22 --23-- 24 --25-- 26 --27-- 28 --29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- ---53-- ---54-- 55 !Samp Bott Bott Pressu Temperat Trans Fluores Fluores Salinity Oxygen Oxyge Numb Tempe Salinity Fl Oxygen: Oxyge Fl Nitra Fl Silic Fl Phosph Fl Ammoni Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: cence: :T1:C1 : n: er_o ratur :Bottle ag Dissolv n: ag te_ ag ate ag ate ag um ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: URU: Dissol Disso ~bin e: ~o ed Disso ~o plus_ ~t ~i ~p ~o Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin Wetlabs ved: lved: _rec Draw tt lved lv Nitri ri ca ha ni ted ct Extrac HP ! ence t SBE SBE ords le ed te te te te um ed ted LC !---- ---- ---- ------ -------- ----- ------- ------- -------- ------ ----- ---- ----- -------- -- ------- ----- -- ----- -- ----- -- ------ -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 358 17 17 6.4 8.9681 37.5 3.900 6.418 31.2617 7.24 315.8 241 8.9 31.2654 0 7.234 315.3 0 4.6 0 11.6 0 0.65 0 0.60 6 6.54 6 0.72 0.339 0.107 0.060 0.846 0.027 0.053 0.072 0.000 0.033 3.027 0.021 0.034 0.018 0.193 0.262 0.122 0.013 0.022 0.000 0.167 0.000 5.426 0.000 0.079 5.586 36 357 16 16 11.2 8.9594 40.1 3.400 5.696 31.3211 7.19 313.3 241 8.8 -99.0000 0 7.187 313.2 0 4.9 0 12.0 0 0.68 0 0.52 6 5.58 6 0.78 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 356 15 15 21.4 8.9058 43.4 2.600 4.783 31.3864 7.07 308.0 241 8.7 -99.0000 0 7.073 308.3 0 5.8 0 12.9 0 0.74 0 0.81 6 4.80 6 0.65 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 355 14 14 31.6 8.8845 44.5 2.400 4.141 31.3954 7.01 305.7 241 8.7 -99.0000 0 7.016 305.8 0 6.4 0 13.4 0 0.79 0 -99.00 0 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1.71 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 351 10 10 125.8 7.0270 67.1 0.000 0.152 33.7510 4.36 189.8 241 7.2 -99.0000 0 4.324 188.1 0 24.9 0 33.6 0 1.76 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 350 9 9 151.5 6.7883 67.3 0.000 0.141 33.8632 3.89 169.2 241 7.0 -99.0000 0 3.880 168.7 0 27.8 0 39.7 0 1.94 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 349 8 8 176.4 6.2965 67.2 0.000 0.141 33.8791 3.22 140.1 241 6.7 -99.0000 0 3.217 139.9 0 31.5 0 47.6 0 2.21 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 348 7 7 201.4 6.0035 67.1 0.000 0.146 33.8761 3.23 140.3 241 6.5 -99.0000 0 3.152 137.1 0 32.4 0 50.9 0 2.25 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 347 6 6 251.5 5.7197 66.9 0.000 0.145 33.9169 2.27 98.9 241 6.3 -99.0000 0 2.248 97.8 0 36.5 0 59.5 0 2.53 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 346 5 5 301.7 5.4877 67.2 0.000 0.140 33.9575 1.67 72.7 241 6.1 -99.0000 0 1.706 74.2 0 39.2 0 67.0 0 2.76 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 345 4 4 401.3 5.2395 67.4 0.000 0.145 34.0574 0.89 38.5 241 5.9 -99.0000 0 0.864 37.6 0 42.7 0 78.6 0 3.05 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 344 3 3 501.1 4.8246 67.6 0.000 0.146 34.1256 0.56 24.4 241 5.9 -99.0000 0 0.566 24.6 6 44.3 6 90.3 6 3.16 6 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 343 2 2 751.6 3.9206 67.7 0.000 0.151 34.2518 0.29 12.8 241 4.9 34.2546 0 0.283 12.3 0 46.1 0 116.0 0 3.24 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 342 1 1 1401.3 2.6214 67.8 0.000 0.144 34.4811 0.50 21.7 241 3.8 -99.0000 0 0.487 21.2 0 46.6 0 156.6 0 3.24 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0