*2021/11/25 13:12:43.44 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2012/05/14 06:24:17.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : EFC76DEA NUMBER OF CHANNELS : 42 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 61 61 2 Bottle_Number n/a 1 1 3 Bottle:Firing_Sequence n/a 1 1 4 Pressure decibar 6.5 6.5 5 Temperature:Secondary 'deg C (ITS90)' 10.9507 10.9507 6 Transmissivity %/metre 57.7 57.7 7 Fluorescence:URU:Seapoint mg/m^3 0.5 0.5 8 Fluorescence:URU:Wetlabs mg/m^3 0.828 0.828 9 PAR uE/m^2/sec 0 0 10 Salinity:T1:C1 PSS-78 30.1207 30.1207 11 Oxygen:Dissolved:SBE mL/L 7.14 7.14 12 Oxygen:Dissolved:SBE umol/kg 311.6 311.6 13 Number_of_bin_records n/a 241 241 14 Chlorophyll:Extracted mg/m^3 0.7 0.7 15 Flag:Chlorophyll:Extracted n/a 16 Phaeo-Pigment:Extracted mg/m^3 0.4E-01 0.4E-01 17 HPLC:Chl-c3 mg/m^3 0.35E-01 0.35E-01 18 HPLC:Chlide-a mg/m^3 0 0 19 HPLC:MgDVP mg/m^3 0.7E-02 0.7E-02 20 HPLC:Chl-c2 mg/m^3 0.71E-01 0.71E-01 21 HPLC:Chl-c1 mg/m^3 0.6E-02 0.6E-02 22 HPLC:Me-chlide mg/m^3 0 0 23 HPLC:Peri mg/m^3 0.17E-01 0.17E-01 24 HPLC:Pheide-a mg/m^3 0 0 25 HPLC:But-fuco mg/m^3 0.4E-02 0.4E-02 26 HPLC:Fuco mg/m^3 0.188 0.188 27 HPLC:Neo mg/m^3 0 0 28 HPLC:Pras mg/m^3 0.8E-02 0.8E-02 29 HPLC:Viola mg/m^3 0.4E-02 0.4E-02 30 HPLC:Hex-fuco mg/m^3 0.74E-01 0.74E-01 31 HPLC:Diadino mg/m^3 0.45E-01 0.45E-01 32 HPLC:Allo mg/m^3 0.11E-01 0.11E-01 33 HPLC:Diato mg/m^3 0.3E-02 0.3E-02 34 HPLC:Zea mg/m^3 0.7E-02 0.7E-02 35 HPLC:Lut mg/m^3 0 0 36 HPLC:Chl-b mg/m^3 0.26E-01 0.26E-01 37 HPLC:DVChl-a mg/m^3 0 0 38 HPLC:Chl-a mg/m^3 0.546 0.546 39 HPLC:Phe mg/m^3 0 0 40 HPLC:B-Car mg/m^3 0.5E-02 0.5E-02 41 HPLC:TChl-a mg/m^3 0.546 0.546 42 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 8 F ' ' 3 9 -99 ' ' 7 F ' ' 1 10 -99 ' ' 9 F ' ' 4 11 -99 ' ' 7 F ' ' 2 12 -99 ' ' 6 F ' ' 1 13 -99 ' ' 5 I I 0 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 3 NQ C ' ' $END $REMARKS Data quality is expressed with the following flags ---------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). ---------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2012-25 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : La Perouse SCIENTIST : Yelland D. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI STATION : LB10 EVENT NUMBER : 27 LATITUDE : 48 18.54000 N ! (deg min) LONGITUDE : 125 41.18000 W ! (deg min) WATER DEPTH : 152 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.21B $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1.2 2012/07/04 09:53:44 241 241 CLEAN 5.0.4 2012/07/04 10:09:48 241 241 ADDSAMP 3.5 2012/07/04 10:40:01 241 241 BINAVE 4.1.1 2012/07/04 10:40:55 241 1 MERGE 3.4 2012/07/31 09:02:24 1 1 CLEAN 5.0.4 2012/07/31 09:02:36 1 1 CALIB 11.7 2012/08/03 12:02:52 1 1 SORT 3.5 2012/08/03 13:50:03 1 1 REMOVECH 8.1.1 2012/08/03 13:52:55 1 1 CHGUNITS 3.0 2012/08/03 13:56:01 1 1 CHGUNITS 3.0 2012/08/03 13:56:19 1 1 REORDER 1.3 2012/08/03 13:59:21 ? ? HDREDIT2 2.5.1 2012/08/06 14:20:08 ? ? CALIB 11.7 2013/06/04 13:18:34 1 1 HDREDIT2 2.6 2013/07/08 08:52:26 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:38:07 1 1 SORT 3.6 2020/08/21 20:01:46 1 1 REORDER 1.3.1 2021/11/25 13:11:21 ? ? MERGE 3.5.1 2021/11/25 13:11:44 1 1 SORT 3.6 2021/11/25 13:11:55 1 1 CLEAN 5.2.4 2021/11/25 13:12:02 1 1 HDREDIT2 3.2 2021/11/25 13:12:24 ? ? HDREDIT2 3.2 2021/11/25 13:12:43 ? ? $END $REMARKS -CLEAN functions: 2012/07/04 10:09:43 20 Reset #RECS, MIN & MAX values in header. Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2012-25\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2012/07/31 09:02:23 2012/07/31 09:02:23 2012/07/31 09:02: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Event_Number, Bottle:Position, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2012-25\processing\hydro\2012-25-0027.samavg Secondary file: P:\Cruise_Data_Processing\2012-25\processing\hydro\2012-25-0027.mrgcln1s -CLEAN functions: 2012/07/31 09:02:11 20 Reduce header to sections: FIL ADM LOC DEP REC INS HIS RAW CAL COM Remarks removed from kept sections. -SORT parameters: 2012/07/31 09:02:15 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2012/07/31 09:02:36 20 Reset #RECS, MIN & MAX values in header. Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2012/08/03 12:02:52 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2012-25\Processing\doc\2012-25-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 17 Salinity:T0:C0 PSS-78 10 0.5000000E-02 0.1000000E+01 18 Salinity:T1:C1 PSS-78 10 0.1500000E-02 0.1000000E+01 -SORT parameters: 2012/08/03 13:50:03 Sorted in ascending order of channel Pressure [dbars] -REMOVECH 2012/08/03 13:52:55 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: - No units changed -HEADER EDITS: 2012/08/06 14:20:08 Applied edit header: P:\Cruise_Data_Processing\2012-25\Processing\doc\2012-25-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 7: Fluorescence:URU:Wetlabs [mg/m^3] Name: Fluorescence:Wetlabs:ECO-AFL ==> Fluorescence:URU:Wetlabs Channel 8: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 9: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 Channel 10: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 15: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Format: F5.1 ==> F8.3 -CALIB parameters: 2013/06/04 13:18:34 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf WARNING: No calibration done on file C:\Data\Nutrients\2012-25\2012-25-0027.cor1 -HEADER EDITS: 2013/07/08 08:52:26 Applied edit header: C:\DATA\Nutrients\doc\headedit-correction.txt -SORT parameters: 2020/08/21 20:01:46 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 13:11:44 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025_Nov2021\Processing\ IOS\2012-025-0027.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025_Nov2021\Processing\ IOS\2012-025-0027.hplc -SORT parameters: 2021/11/25 13:11:55 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 13:12:02 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 13:12:24 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025\2012-025-bot- hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 17: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 13:12:43 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025\2012-025-bot- hdr2.txt $END *COMMENTS Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone for 24 hours at -20C within 10 days of collection and analyzed on a Turner 10AU fluorometer calibrated annually with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “CV%” in file QF2012-25_CHL-*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2012-025_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Brinkmann Dosimat model 765 and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2012-25oxy*.xls. Salinity samples were collected in 200 mL type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and crew caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 1 month after collection. Full details are in file QF2012-25SAL*.xls. Nutrient samples were collected in plastic tubes. One set of samples was collected and and quick frozen in aluminum blocks stored in -20C freezer. These samples were returned to IOS for analysis. Another set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. Theses samples were analyzed at sea during cruise 2012-12 (Line P). All samples were analyzed using the Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For precision and duplicate study see file QF2012-25nuts*.xls. The ammonium method used at IOS is a simplification of the fluorometric technique of Holmes et al, 1999. This method applies to a wide range of ammonium concentrations, uses the addition of a single working reagent and reduces contamination. Typically, samples are collected during the day and stored in the cold room. The working reagent is added to standards and samples at the same time, and the fluorescence is read approximately 8 hours later using a calibrated TD 700 Fluorometer. For full details including a precision study see file 2012-25_NH4*.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Holmes, R.M., Aminto, A., Kérouel, R., Hooker, Bethanie A., and Peterson, Bruce J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Can. J. Fish. Aquat. Sci., 56: 1801-1808. 6. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2. For further processing details see the processing report 2012-25-proc.doc. July 2013: A correction has been applied to Nitrate_plus_Nitrite and Phosphate data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the following report: Corrections to Nitrate and Phosphate for Deep-Water Cruises from 2009 to 2012.pdf which is stored in folder: \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-025_Nov2021\Processing\IOS\2012-025-0027. hplc --------------------------------------------------------------------------- Sample_Number 61: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------- -------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -99 () (0.5E-02 1) Salinity:T1:C1 PSS-78 10 -99 () (0.15E-02 1) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8--- --9--- ---10--- --11-- --12- -13- --14-- 15 --16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- 42 !Samp Bott Bott Pressu Temperat Trans Fluores Fluores PAR Salinity Oxygen Oxyge Numb Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: cence: :T1:C1 : n: er_o phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: URU: Dissol Disso ~bin Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin Wetlabs ved: lved: _rec ted ct Extrac HP ! ence t SBE SBE ords ed ted LC !---- ---- ---- ------ -------- ----- ------- ------- ------ -------- ------ ----- ---- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 61 1 1 6.5 10.9507 57.7 0.500 0.828 0.0 30.1207 7.14 311.6 241 0.70 6 0.04 0.035 0.000 0.007 0.071 0.006 0.000 0.017 0.000 0.004 0.188 0.000 0.008 0.004 0.074 0.045 0.011 0.003 0.007 0.000 0.026 0.000 0.546 0.000 0.005 0.546 36