*2021/11/25 13:09:09.88 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2012/08/22 18:28:11.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 17 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 92E126BC NUMBER OF CHANNELS : 53 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ------------------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 430 446 2 Bottle_Number n/a 1 17 3 Bottle:Firing_Sequence n/a 1 17 4 Pressure decibar 2.1 300.4 5 Temperature:Secondary 'deg C (ITS90)' 4.3203 13.6577 6 Transmissivity %/metre 54.3 67.2 7 Fluorescence:URU:Seapoint mg/m^3 0.61E-01 1.471 8 PAR uE/m^2/sec 0 1340.6 9 Salinity:T1:C1 PSS-78 32.5187 33.9144 10 Oxygen:Dissolved:SBE mL/L 1.83 7.27 11 Oxygen:Dissolved:SBE umol/kg 79.4 316.4 12 Number_of_bin_records n/a 194 241 13 Nitrate_plus_Nitrite umol/L 11.7 39.9 14 Flag:Nitrate_plus_Nitrite n/a 15 Silicate umol/L 19 81.7 16 Flag:Silicate n/a 17 Phosphate umol/L 1.16 2.85 18 Flag:Phosphate n/a 19 Dimethyl_Sulphide nmol/L 0 17.56 20 Flag:Dimethyl_Sulphide n/a 21 Dimethylsulfoniopropionate_Dissolved nmol/L -99 -99 22 Flag:Dimethylsulfoniopropionate_Dissolved ' ' 23 Dimethylsulfoniopropionate_Total nmol/L -99 -99 24 Flag:Dimethylsulfoniopropionate_Total ' ' 25 Chlorophyll:Extracted mg/m^3 0.9E-01 0.3 26 Flag:Chlorophyll:Extracted n/a 27 Phaeo-Pigment:Extracted mg/m^3 0 0.26 28 HPLC:Chl-c3 mg/m^3 0.39E-01 0.102 29 HPLC:Chlide-a mg/m^3 0 0 30 HPLC:MgDVP mg/m^3 0 0.4E-02 31 HPLC:Chl-c2 mg/m^3 0.52E-01 0.74E-01 32 HPLC:Chl-c1 mg/m^3 0 0 33 HPLC:Me-chlide mg/m^3 0 0 34 HPLC:Peri mg/m^3 0.1E-01 0.23E-01 35 HPLC:Pheide-a mg/m^3 0 0 36 HPLC:But-fuco mg/m^3 0.5E-01 0.58E-01 37 HPLC:Fuco mg/m^3 0.37E-01 0.94E-01 38 HPLC:Neo mg/m^3 0 0.7E-02 39 HPLC:Pras mg/m^3 0 0.2E-02 40 HPLC:Viola mg/m^3 0 0.4E-02 41 HPLC:Hex-fuco mg/m^3 0.144 0.253 42 HPLC:Diadino mg/m^3 0.9E-02 0.58E-01 43 HPLC:Allo mg/m^3 0 0 44 HPLC:Diato mg/m^3 0 0.27E-01 45 HPLC:Zea mg/m^3 0.2E-02 0.1E-01 46 HPLC:Lut mg/m^3 0 0.5E-02 47 HPLC:Chl-b mg/m^3 0.18E-01 0.54E-01 48 HPLC:DVChl-a mg/m^3 0 0 49 HPLC:Chl-a mg/m^3 0.299 0.394 50 HPLC:Phe mg/m^3 0 0 51 HPLC:B-Car mg/m^3 0.2E-02 0.5E-02 52 HPLC:TChl-a mg/m^3 0.299 0.394 53 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 6 F R4 1 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 7 F R4 2 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 8 F R4 3 53 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2012-13 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P20 EVENT NUMBER : 58 LATITUDE : 49 33.96000 N ! (deg min) LONGITUDE : 138 39.97000 W ! (deg min) WATER DEPTH : 3947 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0550 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.21B $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1.2 2013/01/24 12:15:48 4027 4027 CLEAN 5.0.4 2013/01/24 12:16:40 4027 4027 ADDSAMP 3.5 2013/01/24 12:19:11 4027 4027 BINAVE 4.1.1 2013/01/24 13:06:06 4027 17 MERGE 3.4 2013/01/24 13:06:17 17 17 CLEAN 5.0.4 2013/01/30 16:56:27 17 17 CALIB 11.7 2013/02/06 13:02:57 17 17 SORT 3.6 2013/02/06 13:38:52 17 17 REMOVECH 8.1.1 2013/02/06 13:42:44 17 17 CHGUNITS 3.0 2013/02/06 13:49:43 17 17 CHGUNITS 3.0 2013/02/06 13:49:58 17 17 REORDER 1.3 2013/02/06 13:51:01 ? ? HDREDIT2 2.6 2013/02/06 14:49:10 ? ? CALIB 11.7 2013/06/04 13:20:14 17 17 HDREDIT2 2.6 2013/07/08 08:52:15 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:37:45 17 17 CHANGE_FLAGS 2.0 2014/11/20 09:58:07 17 17 SORT 3.6 2015/07/16 11:13:38 17 17 MERGE 3.5 2015/07/16 11:13:48 17 17 SORT 3.6 2015/07/16 11:14:00 17 17 SORT 3.6 2017/04/11 10:33:42 17 17 REMOVECH 8.2 2020/07/27 11:58:53 17 17 SORT 3.6 2020/07/27 11:59:40 17 17 MERGE 3.5.1 2020/07/27 12:00:44 17 17 SORT 3.6 2020/07/27 12:08:40 17 17 CLEAN 5.2.4 2020/07/27 12:12:07 17 17 SORT 3.6 2020/08/27 19:26:04 17 17 REORDER 1.3.1 2021/11/25 13:07:58 ? ? MERGE 3.5.1 2021/11/25 13:08:10 17 17 SORT 3.6 2021/11/25 13:08:18 17 17 $END $REMARKS -CLEAN functions: 2013/01/24 12:16:13 20 Interpolation by Channel 1: Scan_Number [n/a] in Pressure Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2012-13\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2013/01/24 13:06:16 2013/01/24 13:06:16 2013/01/24 13:06: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Event_Number, Bottle:Position, Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2012-13\Processing\ios\2012-13-0058.samavg Secondary file: P:\Cruise_Data_Processing\2012-13\Processing\ios\2012-13-0058.mrgcln1s -SORT parameters: 2013/01/22 14:40:20 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2013/01/30 16:56:25 20 Reset #RECS, MIN & MAX values in header. Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2013/02/06 13:02:57 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2012-13\Processing\doc\2012-13-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 18 Oxygen:Dissolved:SBE mL/L 10 0.1590000E-01 0.1043500E+01 -SORT parameters: 2013/02/06 13:38:52 Sorted in ascending order of channel Pressure -REMOVECH 2013/02/06 13:42:44 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: - No units changed -HEADER EDITS: 2013/02/06 14:49:10 Applied edit header: P:\Cruise_Data_Processing\2012-13\Processing\doc\HYDRO\2012-13-bot-hdr.txt Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 2: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 7: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 8: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 Channel 9: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 13: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 14: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 15: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 16: Nitrate_plus_Nitrite [umol/L] Units: ==> umol/L Channel 17: Flag:Nitrate_plus_Nitrite [n/a] Units: ==> n/a Channel 18: Silicate [umol/L] Units: ==> umol/L Channel 19: Flag:Silicate [n/a] Units: ==> n/a Channel 20: Phosphate [umol/L] Units: ==> umol/L Channel 21: Flag:Phosphate [n/a] Units: ==> n/a Channel 22: Dimethyl_Sulphide [nmol/L] Units: ==> nmol/L Channel 23: Flag:Dimethyl_Sulphide [n/a] Units: ==> n/a Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint -CALIB parameters: 2013/06/04 13:20:14 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 16 Nitrate_plus_Nitrite umol/L 10 0.1709000E+00 0.9812000E+00 20 Phosphate umol/L 10 0.4530000E-01 0.1011500E+01 -HEADER EDITS: 2013/07/08 08:52:15 Applied edit header: C:\DATA\Nutrients\doc\headedit-correction.txt -SORT parameters: 2015/07/16 11:13:38 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2015/07/16 11:13:48 Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolve, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total Primary file : P:\Cruise_Data_Processing\DMSP\2012-13\Bottle\2012-13-0058.che1 Secondary file: P:\Cruise_Data_Processing\DMSP\2012-13\Bottle\2012-13-0058.dmsp -SORT parameters: 2015/07/16 11:14:00 Sorted in ascending order of channel Sample_Number -SORT parameters: 2017/04/11 10:33:42 Sorted in ascending order of channel Pressure -REMOVECH 2020/07/27 11:58:53 The following CHANNEL(S) were removed: Dimethylsulfoniopropionate_Dissolved [nmol/L] Flag:Dimethylsulfoniopropionate_Dissolve Dimethylsulfoniopropionate_Total [nmol/L] Flag:Dimethylsulfoniopropionate_Total -SORT parameters: 2020/07/27 11:59:40 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2020/07/27 12:00:44 Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Dimethylsulfoniopropionate_Dissolved, Flag:Dimethylsulfoniopropionate_Dissolved, Dimethylsulfoniopropionate_Total, Flag:Dimethylsulfoniopropionate_Total Primary file : Q:\OSD_DataProcessing\Cruise_Data\DMSP\Joe's Work\2012-013\Processing\2012-013- 0058.sort1 Secondary file: Q:\OSD_DataProcessing\Cruise_Data\DMSP\Joe's Work\2012-013\Processing\2012-013- 0058.dmsp -SORT parameters: 2020/07/27 12:08:40 Sorted in ascending order of channel Sample_Number -CLEAN functions: 2020/07/27 12:11:38 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag* -SORT parameters: 2020/08/27 19:26:04 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 13:08:10 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-013_Nov2021\Processing\ IOS\2012-013-0058.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-013_Nov2021\Processing\ IOS\2012-013-0058.hplc -SORT parameters: 2021/11/25 13:08:18 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 13:08:25 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 13:08:49 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-013\2012-013-bot- hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 28: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 52: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 13:09:09 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-013\2012-013-bot- hdr2.txt $END *COMMENTS Sample_Number 436: CHL:CV>15-50% Comments from secondary file: Q:\OSD_DataProcessing\Cruise_Data\DMSP\Joe's Work\2012-013\Processing\2012-013-0058.dmsp --------------------------------------------------------------------------- Sample_Number 431: DMSP-D & DMSP-T: Elevated results due to contamination Sample_Number 432: DMSP-D & DMSP-T: Elevated results due to contamination Sample_Number 438: DMSP-D & DMSP-T: Elevated results due to contamination Sample_Number 441: DMSP-D & DMSP-T: Elevated results due to contamination Sample_Number 446: DMSP-D & DMSP-T: Elevated results due to contamination Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “Precision” in file QF2012-13CHL*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. Note: The variability between replicates was higher than usual and the values are lower than they typically are in comparison to HPLC chl. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6-7 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2012-013_HPLC*.xlsx. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document QF2012-13OXY*.xls. Salinity samples were collected in 200 mL type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater within 1.6 months after collection. Full details including a duplicate analysis are in file QF2012-13SAL*.xls. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples from 400m and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. THey were analyzed using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For raw data, precision and duplicate details see worksheet QF2012-13nuts*.xls. The minimum detectable level for DMS is 0.10 nM, so “0” values should be interpreted as <0.10. For details on the collection and analysis of the DMS data see file 2012-13DMS Report.doc and for data see 2012-13DMS*.xls. The minimum detectable level for DMSP in this file is 0.1 nmol/L, so “0” values should be interpreted as <0.10 nmol/L. For details on analysis and raw data, see document "2012-13 dmsp QF summary.xls. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2 with a modification allowing a small offset. For further processing details see the processing report 2012-13-proc.doc. July 2013: A correction has been applied to Nitrate_plus_Nitrite and Phosphate data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the following report: Corrections to Nitrate and Phosphate for Deep-Water Cruises from 2009 to 2012.pdf which is stored in folder: \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2012\2012-013_Nov2021\Processing\IOS\2012-013-0058. hplc --------------------------------------------------------------------------- Sample_Number 445: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- -------- ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.159E-01 1.0435) Nitrate_plus_Nitrite umol/L 10 -99 () (0.1709 0.9812) Phosphate umol/L 10 -99 () (0.453E-01 1.0115) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- --8--- ---9---- --10-- --11- -12- --13- 14 --15- 16 --17-- 18 --19-- 20 --21-- 22 --23-- 24 --25-- 26 --27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- ---52-- 53 !Samp Bott Bott Pressu Temperat Trans Fluores PAR Salinity Oxygen Oxyge Numb Nitra Fl Silic Fl Phosph Fl Dimeth Fl Dimeth Fl Dimeth Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: :T1:C1 : n: er_o te_ ag ate ag ate ag yl_ ag ylsulf ag ylsulf ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: Dissol Disso ~bin plus_ ~t ~i ~p Sulphi ~p ~piona ~o ~oprop ~T Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin ved: lved: _rec Nitri ri ca ha de hi te_Dis lv ionate ot ted ct Extrac HP ! ence t SBE SBE ords te te te te de solved ed _Total al ed ted LC !---- ---- ---- ------ -------- ----- ------- ------ -------- ------ ----- ---- ----- -- ----- -- ------ -- ------ -- ------ -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 442 17 17 2.1 13.6562 54.3 0.213 1340.6 32.5199 6.22 271.2 241 11.7 0 19.0 0 1.16 0 16.71 0 -99.00 0 -99.00 0 0.25 0 0.06 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 441 12 14 5.5 13.6577 54.5 0.214 778.2 32.5196 6.21 270.8 194 11.8 0 19.0 0 1.19 0 12.15 0 -99.00 5 -99.00 5 0.28 0 0.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 445 16 15 5.6 13.6570 54.5 0.210 742.2 32.5196 6.22 271.1 218 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 0 -99.00 0 0.29 6 0.06 0.039 0.000 0.000 0.052 0.000 0.000 0.020 0.000 0.055 0.037 0.000 0.000 0.000 0.144 0.058 0.000 0.027 0.002 0.000 0.018 0.000 0.299 0.000 0.005 0.299 36 446 15 16 5.7 13.6524 54.5 0.223 762.5 32.5195 6.22 271.1 241 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 5 -99.00 5 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 440 11 13 10.8 13.6453 54.5 0.376 392.9 32.5197 6.22 271.2 241 11.7 0 19.0 0 1.16 0 13.57 0 -99.00 0 -99.00 0 0.21 0 0.06 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 439 10 12 15.7 13.5941 54.4 0.548 271.9 32.5187 6.24 271.9 241 11.7 0 19.1 0 1.17 0 16.40 0 -99.00 0 -99.00 0 0.21 0 0.07 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 438 9 11 20.2 12.8771 56.9 0.647 145.1 32.5231 6.39 278.4 241 11.9 0 19.7 0 1.20 0 17.56 0 -99.00 5 -99.00 5 0.27 0 0.04 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 437 8 10 25.8 12.2993 57.4 0.796 108.6 32.5471 6.48 282.4 241 12.0 0 19.6 0 1.21 0 15.33 0 -99.00 0 -99.00 0 0.21 0 0.09 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 436 7 9 31.1 10.6720 58.7 0.833 55.9 32.5977 6.83 297.5 241 12.4 0 20.3 0 1.24 0 14.90 0 -99.00 0 -99.00 0 0.26 36 0.09 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 435 6 8 40.6 8.9656 60.2 0.897 29.5 32.6242 7.09 308.9 241 13.0 0 22.6 0 1.28 0 10.83 0 -99.00 0 -99.00 0 0.16 0 0.11 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 434 5 7 50.7 7.5636 59.2 1.444 16.6 32.6482 7.22 314.4 241 13.7 0 23.8 0 1.32 0 4.32 0 -99.00 0 -99.00 0 0.30 0 0.18 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 444 15 6 55.5 6.8358 59.8 1.471 12.3 32.6661 7.27 316.4 241 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 0 -99.00 0 0.30 6 0.17 0.102 0.000 0.004 0.074 0.000 0.000 0.023 0.000 0.050 0.094 0.006 0.000 0.000 0.253 0.019 0.000 0.001 0.004 0.003 0.050 0.000 0.394 0.000 0.002 0.394 6 443 14 5 65.4 5.9811 62.5 1.070 6.1 32.6926 7.23 314.6 241 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 0 -99.00 0 0.24 6 0.18 0.092 0.000 0.004 0.054 0.000 0.000 0.010 0.000 0.058 0.071 0.007 0.002 0.004 0.200 0.009 0.000 0.000 0.010 0.005 0.054 0.000 0.341 0.000 0.002 0.341 6 433 4 4 75.8 5.4602 64.5 0.598 3.1 32.7205 7.03 306.0 241 16.6 0 26.4 0 1.49 0 0.45 0 -99.00 0 -99.00 0 0.09 0 0.26 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 432 3 3 100.6 4.7373 66.3 0.251 0.8 32.8240 6.71 291.8 241 19.5 0 30.2 0 1.52 0 0.39 0 -99.00 5 -99.00 5 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 431 2 2 200.6 4.6215 66.9 0.062 0.0 33.7833 2.99 129.8 241 35.1 6 65.7 6 2.48 6 0.00 0 -99.00 5 -99.00 5 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 430 1 1 300.4 4.3203 67.2 0.061 0.0 33.9144 1.83 79.4 241 39.9 0 81.7 0 2.85 0 0.00 0 -99.00 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0