*2021/11/25 10:36:49.64 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2011/04/16 15:51:48.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 14 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : E970694C NUMBER OF CHANNELS : 52 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 163 176 2 Bottle:Firing_Sequence n/a 1 14 3 Pressure decibar 3 251.2 4 Temperature:Secondary 'deg C (ITS90)' 6.7947 8.3999 5 Transmissivity %/metre 27.1 55.6 6 Fluorescence:URU:Seapoint mg/m^3 0.178 2.02 7 PAR uE/m^2/sec 0 393.5 8 PAR:Reference uE/m^2/sec 248.9 882.8 9 Salinity:T1:C1 PSS-78 29.1356 33.907 10 Oxygen:Dissolved:SBE mL/L 1.92 6.9 11 Oxygen:Dissolved:SBE umol/kg 83.6 301.3 12 Number_of_bin_records n/a 153 241 13 Temperature:Draw 'deg C (ITS90)' 6.8 8.2 14 Salinity:Bottle PSS-78 33.9062 33.9062 15 Flag:Salinity:Bottle n/a 16 Oxygen:Dissolved mL/L 1.913 6.905 17 Oxygen:Dissolved umol/kg 83.2 301.4 18 Flag:Oxygen:Dissolved n/a 19 Nitrate_plus_Nitrite umol/L 9.5 35.4 20 Flag:Nitrate_plus_Nitrite n/a 21 Silicate umol/L 22.8 53.8 22 Flag:Silicate n/a 23 Phosphate umol/L 0.8 2.48 24 Flag:Phosphate n/a 25 Chlorophyll:Extracted mg/m^3 0.75 2.78 26 Flag:Chlorophyll:Extracted n/a 27 Phaeo-Pigment:Extracted mg/m^3 1.14 1.18 28 Bottle:Firing_Sequence n/a 1 14 29 HPLC:Chl-c3 mg/m^3 0.26E-01 0.57E-01 30 HPLC:Chlide-a mg/m^3 0.37E-01 0.43E-01 31 HPLC:MgDVP mg/m^3 0.17E-01 0.34E-01 32 HPLC:Chl-c2 mg/m^3 0.133 0.285 33 HPLC:Peri mg/m^3 0.21E-01 0.105 34 HPLC:Pheide-a mg/m^3 0 0 35 HPLC:But-fuco mg/m^3 0.4E-02 0.23E-01 36 HPLC:Fuco mg/m^3 0.681 1.278 37 HPLC:Neo mg/m^3 0.9E-02 0.14E-01 38 HPLC:Pras mg/m^3 0.15E-01 0.2E-01 39 HPLC:Viola mg/m^3 0.6E-02 0.18E-01 40 HPLC:Hex-fuco mg/m^3 0.7E-02 0.32E-01 41 HPLC:Diadino mg/m^3 0.46E-01 0.155 42 HPLC:Allo mg/m^3 0.21E-01 0.87E-01 43 HPLC:Diato mg/m^3 0.4E-02 0.11E-01 44 HPLC:Zea mg/m^3 0.7E-02 0.1E-01 45 HPLC:Lut mg/m^3 0.5E-02 0.6E-02 46 HPLC:Chl-b mg/m^3 0.6E-01 0.104 47 HPLC:DVChl-a mg/m^3 0 0 48 HPLC:Chl-a mg/m^3 1.191 2.413 49 HPLC:Phe mg/m^3 0 0.104 50 HPLC:B-Car mg/m^3 0.31E-01 0.53E-01 51 HPLC:TChl-a mg/m^3 1.228 2.455 52 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 9 F R4 4 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 6 F ' ' 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 6 F R4 1 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 6 F R4 1 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 5 I I 0 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2011-08 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : SoG/JdeF Water Properties Survey SCIENTIST : Chandler P. PLATFORM : Vector *LOCATION GEOGRAPHIC AREA : Strait of Georgia / Juan de Fuca Strait STATION : 102 EVENT NUMBER : 45 LATITUDE : 48 30.03000 N ! (deg min) LONGITUDE : 124 43.98000 W ! (deg min) WATER DEPTH : 256 ALTIMETER (M) : 7.25 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0941 $REMARKS SOFTWARE VERSION SEASAVE V 7.20G $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1 2011/09/29 14:52:23 3286 3286 CLEAN 5.0.3 2011/09/29 14:53:00 ? ? ADDSAMP 3.5 2011/09/29 15:28:02 3286 3286 BINAVE 4.1.1 2011/09/29 15:28:24 3286 14 MERGE 3.4 2011/10/05 09:15:35 14 14 CLEAN 5.0.3 2011/10/05 09:15:42 ? ? CALIB 11.7 2011/10/05 09:15:49 14 14 SORT 3.5 2011/10/05 09:16:05 14 14 REMOVECH 8.0 2011/10/05 09:16:10 14 14 CHGUNITS 3.0 2011/10/05 09:16:47 14 14 CHGUNITS 3.0 2011/10/05 09:17:11 14 14 REORDER 1.3 2011/10/05 09:17:21 ? ? HDREDIT2 2.5.1 2011/10/05 09:17:34 ? ? HDREDIT2 2.5.1 2011/10/10 15:38:50 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:35:25 14 14 CHANGE_FLAGS 2.0 2014/11/20 09:57:31 14 14 SORT 3.6 2020/11/30 14:17:33 14 14 REORDER 1.3.1 2021/11/25 10:35:31 ? ? MERGE 3.5.1 2021/11/25 10:35:40 14 14 SORT 3.6 2021/11/25 10:35:47 14 14 CLEAN 5.2.4 2021/11/25 10:35:55 14 14 HDREDIT2 3.2 2021/11/25 10:36:27 ? ? HDREDIT2 3.2 2021/11/25 10:36:49 ? ? $END $REMARKS -CLEAN functions: 2011/09/29 14:52:45 20 Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2011-08\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2011/10/05 09:15:33 2011/10/05 09:15:33 2011/10/05 09:15: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0045.samavg Secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0045.mrgcln1 -CLEAN functions: 2011/10/05 09:15:41 20 Remove Sea-Bird comments from the header. -CALIB parameters: 2011/10/05 09:15:49 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2011-08\Processing\doc\2011-08-recal.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 3 Pressure dbars 10 -0.9039000E+00 0.1000000E+01 12 PAR:Reference uE/m^2/se 10 0.0000000E+00 0.1527282E+04 -SORT parameters: 2011/10/05 09:16:05 Sorted in ascending order of channel Pressure -REMOVECH 2011/10/05 09:16:10 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2011/10/05 09:17:34 Applied edit header: P:\Cruise_Data_Processing\2011-08\Processing\doc\2011-08-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 7: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 10: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 17: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 19: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 20: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 25: Silicate [umol/L] Format: F7.2 ==> F6.1 Channel 8: PAR:Reference [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 11: Oxygen:Dissolved:SBE [umol/kg] Format: F6.2 ==> F6.1 Channel 21: Oxygen:Dissolved [umol/kg] Format: F6.2 ==> F6.1 -HEADER EDITS: 2011/10/10 15:38:50 Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 9: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 -SORT parameters: 2020/11/30 14:17:33 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 10:35:40 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008_Nov2021\Processing\ IOS\2011-008-0045.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008_Nov2021\Processing\ IOS\2011-008-0045.hplc -SORT parameters: 2021/11/25 10:35:47 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 10:35:53 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 10:36:27 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008-bot-hdr1.txt Channel 2: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 28: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 13: Temperature:Draw [deg C (ITS90)] Format: F9.4 ==> F6.1 Channel 29: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 10:36:49 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008-bot-hdr2.txt $END *COMMENTS Comments from secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0045.mrgcln1 ----------------------------------------------------------------------- Comments from secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0045.chl ------------------------------------------------------------------- Sample_Number 173: CHL: variability of reps >15% CV Comments from secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0045.nut ------------------------------------------------------------------- Sample_Number 164: not tripped at target depth, see rosette log Sample_Number 165: not tripped at target depth, see rosette log Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab within a week of collection and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab and at IOS. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “CV%” in file 2011-08_CHL*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~3 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a and Chlide-a For further information see file QF 2011-008_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Brinkmann Dosimat model 665 and a UV light source and detector with a 365 nm filter controlled by LVO2 software designed and built by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document 2011-08oxy*.xls. Salinity samples were collected in glass bottles and analyzed on Guildline Autosal model 8400B, S/N 68572, at IOS. Salinometers are standardized with IAPSO standard seawater. Full results are in file 2011-08-sal*.xls. Nutrient samples were collected in plastic tubes. Samples were stored frozen and later analysed at IOS. An Astoria analyzer was used following methods described in Barwell-Clarke and Whitney (1996). File "QF 2011-08nuts*.xls" includes the raw data and a precision study. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, PAR and SBE Fluorescence data are nominal and unedited. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2. For further processing details see the processing report 2011-08-proc.doc. Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008_Nov2021\Processing\IOS\2011-008-0045. hplc --------------------------------------------------------------------------- Sample_Number 173: HPLC: >10%CV for TChl-a Sample_Number 176: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! --------------- ------------ ---- ------ ------------ Pressure dbars 10 -99 () (-0.9039 1) PAR:Reference uE/m^2/sec 10 -99 () (0 1527.282) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- --8--- ---9---- --10-- --11- -12- --13- ---14--- 15 ---16-- --17- 18 --19- 20 --21- 22 --23-- 24 --25-- 26 --27-- -28- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- 52 !Samp Bott Pressu Temperat Trans Fluores PAR PAR: Salinity Oxygen Oxyge Numb Tempe Salinity Fl Oxygen: Oxyge Fl Nitra Fl Silic Fl Phosph Fl Chloro Fl Phaeo- Bott HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le:F re ure: missi cence: Refere :T1:C1 : n: er_o ratur :Bottle ag Dissolv n: ag te_ ag ate ag ate ag phyll: ag Pigmen le:F Chl-c3 Chlide- MgDVP Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb ~ng_ Secondar vity URU: nce Dissol Disso ~bin e: ~o ed Disso ~o plus_ ~t ~i ~p Extrac ~a t: ~ng_ a a fuco fuco : !er Sequ y Seapoin ved: lved: _rec Draw tt lved lv Nitri ri ca ha ted ct Extrac Sequ HP ! ence t SBE SBE ords le ed te te te te ed ted ence LC !---- ---- ------ -------- ----- ------- ------ ------ -------- ------ ----- ---- ----- -------- -- ------- ----- -- ----- -- ----- -- ------ -- ------ -- ------ ---- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 176 14 3.0 8.3999 42.0 1.269 393.5 882.8 29.1511 6.90 301.3 241 8.2 -99.0000 0 6.897 301.1 0 9.6 0 22.9 0 0.80 0 2.12 6 1.14 14 0.026 0.040 0.034 0.238 0.105 0.000 0.023 1.072 0.014 0.020 0.018 0.032 0.155 0.087 0.011 0.010 0.006 0.081 0.000 2.238 0.000 0.053 2.278 36 175 13 3.7 8.3984 42.0 1.449 291.8 829.3 29.1356 6.87 300.1 241 8.1 -99.0000 0 6.905 301.4 0 9.5 0 22.8 0 0.92 0 -99.00 0 -99.00 13 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 174 12 9.2 8.3347 43.1 2.020 50.1 754.5 29.9746 6.57 286.5 241 8.1 -99.0000 0 6.662 290.6 0 12.0 0 25.1 0 1.00 0 2.78 6 1.18 12 0.057 0.043 0.032 0.285 0.064 0.000 0.006 1.278 0.011 0.017 0.013 0.023 0.117 0.045 0.011 0.010 0.005 0.104 0.000 2.413 0.104 0.053 2.455 6 173 11 18.5 8.1274 47.1 1.436 8.9 719.3 30.3980 6.32 275.4 241 7.9 -99.0000 0 6.332 276.1 0 18.2 0 33.9 0 1.43 0 0.75 3 1.14 11 0.026 0.037 0.017 0.133 0.021 0.000 0.004 0.681 0.009 0.015 0.006 0.007 0.046 0.021 0.004 0.007 0.005 0.060 0.000 1.191 0.000 0.031 1.228 36 172 10 29.5 7.9758 48.0 1.227 1.3 696.4 30.6684 6.13 267.1 241 7.7 -99.0000 0 6.190 269.9 0 21.6 6 39.2 6 1.68 6 -99.00 0 -99.00 10 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 171 9 40.8 7.9259 48.5 1.016 0.2 661.3 30.7508 5.93 258.7 241 7.7 -99.0000 0 5.959 259.8 6 22.8 0 40.6 0 1.68 0 -99.00 0 -99.00 9 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 170 8 51.8 7.8707 48.8 0.819 0.0 598.7 30.9194 5.69 248.1 241 7.6 -99.0000 0 5.754 250.8 0 22.7 0 39.6 0 1.75 0 -99.00 0 -99.00 8 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 169 7 73.5 7.7593 43.4 0.377 0.0 554.4 31.5790 5.01 218.2 241 7.5 -99.0000 0 5.038 219.5 0 23.9 0 39.3 0 1.86 0 -99.00 0 -99.00 7 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 168 6 103.4 7.9862 49.0 0.255 0.0 490.3 32.9051 4.15 180.5 241 7.7 -99.0000 0 4.311 187.6 0 21.0 0 30.6 0 1.66 0 -99.00 0 -99.00 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 167 5 121.7 7.4866 52.6 0.221 0.0 438.3 33.5676 2.85 124.1 241 7.3 -99.0000 0 2.886 125.5 0 29.2 0 41.6 0 2.12 0 -99.00 0 -99.00 5 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 166 4 146.5 7.3009 52.6 0.198 0.0 398.6 33.7120 2.53 110.0 241 7.1 -99.0000 0 2.530 110.0 6 31.4 0 45.1 0 2.25 0 -99.00 0 -99.00 4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 165 3 147.0 7.3012 53.4 0.201 0.0 392.5 33.7124 2.53 110.0 153 7.1 -99.0000 0 2.584 112.4 0 31.4 0 45.1 0 2.26 0 -99.00 0 -99.00 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 164 2 174.5 7.1725 55.6 0.178 0.0 342.1 33.7982 2.32 100.9 241 7.1 -99.0000 0 2.286 99.4 0 32.8 0 47.3 0 2.33 0 -99.00 0 -99.00 2 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 163 1 251.2 6.7947 27.1 0.195 0.0 248.9 33.9070 1.92 83.6 241 6.8 33.9062 0 1.913 83.2 0 35.4 0 53.8 0 2.48 0 -99.00 0 -99.00 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0