*2021/11/25 10:36:48.95 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2011/04/14 17:51:22.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 16 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 27185A22 NUMBER OF CHANNELS : 52 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 28 43 2 Bottle:Firing_Sequence n/a 1 16 3 Pressure decibar 3.2 350.8 4 Temperature:Secondary 'deg C (ITS90)' 7.9951 9.259 5 Transmissivity %/metre 32.5 59.3 6 Fluorescence:URU:Seapoint mg/m^3 0.87E-01 8.032 7 PAR uE/m^2/sec 0 389.2 8 PAR:Reference uE/m^2/sec 972.9 1959.5 9 Salinity:T1:C1 PSS-78 28.6765 30.7598 10 Oxygen:Dissolved:SBE mL/L 3.02 7.87 11 Oxygen:Dissolved:SBE umol/kg 131.5 343.8 12 Number_of_bin_records n/a 241 241 13 Temperature:Draw 'deg C (ITS90)' 7.8 8.6 14 Salinity:Bottle PSS-78 30.6845 30.6845 15 Flag:Salinity:Bottle n/a 16 Oxygen:Dissolved mL/L 3.071 7.881 17 Oxygen:Dissolved umol/kg 133.9 344.2 18 Flag:Oxygen:Dissolved n/a 19 Nitrate_plus_Nitrite umol/L 13.1 30.3 20 Flag:Nitrate_plus_Nitrite n/a 21 Silicate umol/L 24.2 68.6 22 Flag:Silicate n/a 23 Phosphate umol/L 1.38 2.73 24 Flag:Phosphate n/a 25 Chlorophyll:Extracted mg/m^3 16.62 18.76 26 Flag:Chlorophyll:Extracted n/a 27 Phaeo-Pigment:Extracted mg/m^3 1.32 3.15 28 Bottle:Firing_Sequence n/a 1 16 29 HPLC:Chl-c3 mg/m^3 0.34E-01 0.66E-01 30 HPLC:Chlide-a mg/m^3 0.654 0.809 31 HPLC:MgDVP mg/m^3 0.104 0.128 32 HPLC:Chl-c2 mg/m^3 1.072 1.373 33 HPLC:Peri mg/m^3 0.83E-01 0.9E-01 34 HPLC:Pheide-a mg/m^3 0 0.184 35 HPLC:But-fuco mg/m^3 0 0.3E-01 36 HPLC:Fuco mg/m^3 6.332 7.989 37 HPLC:Neo mg/m^3 0 0 38 HPLC:Pras mg/m^3 0 0 39 HPLC:Viola mg/m^3 0.6E-02 0.11E-01 40 HPLC:Hex-fuco mg/m^3 0 0 41 HPLC:Diadino mg/m^3 0.45 0.7 42 HPLC:Allo mg/m^3 0.5E-02 0.16E-01 43 HPLC:Diato mg/m^3 0.31E-01 0.57E-01 44 HPLC:Zea mg/m^3 0.35E-01 0.45E-01 45 HPLC:Lut mg/m^3 0 0 46 HPLC:Chl-b mg/m^3 0 0.11E-01 47 HPLC:DVChl-a mg/m^3 0 0 48 HPLC:Chl-a mg/m^3 8.712 11.98 49 HPLC:Phe mg/m^3 0 0.2 50 HPLC:B-Car mg/m^3 0.218 0.28 51 HPLC:TChl-a mg/m^3 9.522 12.776 52 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 6 F R4 1 14 -99 ' ' 9 F R4 4 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 6 F ' ' 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 6 F R4 1 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 6 F R4 1 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 -99 ' ' 3 NQ C ' ' 27 -99 ' ' 7 F R4 2 28 -99 ' ' 5 I I 0 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2011-08 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : SoG/JdeF Water Properties Survey SCIENTIST : Chandler P. PLATFORM : Vector *LOCATION GEOGRAPHIC AREA : Strait of Georgia / Juan de Fuca Strait STATION : 12 EVENT NUMBER : 12 LATITUDE : 49 43.60000 N ! (deg min) LONGITUDE : 124 40.80000 W ! (deg min) WATER DEPTH : 355 ALTIMETER (M) : 5.86 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0941 $REMARKS SOFTWARE VERSION SEASAVE V 7.20G $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1 2011/09/29 14:52:07 3856 3856 CLEAN 5.0.3 2011/09/29 14:52:48 ? ? ADDSAMP 3.5 2011/09/29 15:27:49 3856 3856 BINAVE 4.1.1 2011/09/29 15:28:18 3856 16 MERGE 3.4 2011/10/05 09:15:34 16 16 CLEAN 5.0.3 2011/10/05 09:15:41 ? ? CALIB 11.7 2011/10/05 09:15:48 16 16 SORT 3.5 2011/10/05 09:16:05 16 16 REMOVECH 8.0 2011/10/05 09:16:10 16 16 CHGUNITS 3.0 2011/10/05 09:16:47 16 16 CHGUNITS 3.0 2011/10/05 09:17:10 16 16 REORDER 1.3 2011/10/05 09:17:19 ? ? HDREDIT2 2.5.1 2011/10/05 09:17:32 ? ? HDREDIT2 2.5.1 2011/10/10 15:38:36 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:35:25 16 16 CHANGE_FLAGS 2.0 2014/11/20 09:57:31 16 16 SORT 3.6 2020/11/30 14:17:33 16 16 REORDER 1.3.1 2021/11/25 10:35:31 ? ? MERGE 3.5.1 2021/11/25 10:35:39 16 16 SORT 3.6 2021/11/25 10:35:47 16 16 CLEAN 5.2.4 2021/11/25 10:35:54 16 16 HDREDIT2 3.2 2021/11/25 10:36:26 ? ? HDREDIT2 3.2 2021/11/25 10:36:48 ? ? $END $REMARKS -CLEAN functions: 2011/09/29 14:52:45 20 Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: P:\Cruise_Data_Processing\2011-08\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2011/10/05 09:15:33 2011/10/05 09:15:33 2011/10/05 09:15: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Temperature:Draw, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Flag:Dimethyl_Sulphide Primary file : P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0012.samavg Secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0012.mrgcln1 -CLEAN functions: 2011/10/05 09:15:41 20 Remove Sea-Bird comments from the header. -CALIB parameters: 2011/10/05 09:15:48 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = P:\Cruise_Data_Processing\2011-08\Processing\doc\2011-08-recal.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 3 Pressure dbars 10 -0.9039000E+00 0.1000000E+01 12 PAR:Reference uE/m^2/se 10 0.0000000E+00 0.1527282E+04 -SORT parameters: 2011/10/05 09:16:05 Sorted in ascending order of channel Pressure -REMOVECH 2011/10/05 09:16:10 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Status:Pump Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -CHANGE units: 'Oxygen:Dissolved' changed from mL/L to umol/kg -HEADER EDITS: 2011/10/05 09:17:32 Applied edit header: P:\Cruise_Data_Processing\2011-08\Processing\doc\2011-08-bot-hdr.txt Channel 2: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 3: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 6: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 7: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 1: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 10: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 17: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 19: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 20: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 25: Silicate [umol/L] Format: F7.2 ==> F6.1 Channel 8: PAR:Reference [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 11: Oxygen:Dissolved:SBE [umol/kg] Format: F6.2 ==> F6.1 Channel 21: Oxygen:Dissolved [umol/kg] Format: F6.2 ==> F6.1 -HEADER EDITS: 2011/10/10 15:38:36 Channel 1: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 9: Salinity:Practical:T1:C1 [PSS-78] Name: Salinity:T1:C1 ==> Salinity:Practical:T1:C1 -SORT parameters: 2020/11/30 14:17:33 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/25 10:35:39 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008_Nov2021\Processing\ IOS\2011-008-0012.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008_Nov2021\Processing\ IOS\2011-008-0012.hplc -SORT parameters: 2021/11/25 10:35:47 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/25 10:35:53 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/25 10:36:26 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008-bot-hdr1.txt Channel 2: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 28: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 13: Temperature:Draw [deg C (ITS90)] Format: F9.4 ==> F6.1 Channel 29: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 50: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 51: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/25 10:36:48 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008-bot-hdr2.txt $END *COMMENTS Comments from secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0012.mrgcln1 ----------------------------------------------------------------------- Comments from secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0012.chl ------------------------------------------------------------------- Sample_Number 40: CHL: variability of reps >15% CV Sample_Number 43: CHL: variability of reps >15% CV Comments from secondary file: P:\Cruise_Data_Processing\2011-08\Processing\hydro\2011-08-0012.oxy ------------------------------------------------------------------- Sample_Number 31: DO: poor replicates; Looks ok in comparison with SBE DO. Sample_Number 32: DO: stopper not seated properly; Looks ok in comparison with SBE DO. Analysis methods: ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours in the lab within a week of collection and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab and at IOS. The average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see worksheet “CV%” in file 2011-08_CHL*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~3 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a and Chlide-a For further information see file QF 2011-008_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system (Brinkmann Dosimat model 665 and a UV light source and detector with a 365 nm filter controlled by LVO2 software designed and built by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details including a duplicate analysis, see document 2011-08oxy*.xls. Salinity samples were collected in glass bottles and analyzed on Guildline Autosal model 8400B, S/N 68572, at IOS. Salinometers are standardized with IAPSO standard seawater. Full results are in file 2011-08-sal*.xls. Nutrient samples were collected in plastic tubes. Samples were stored frozen and later analysed at IOS. An Astoria analyzer was used following methods described in Barwell-Clarke and Whitney (1996). File "QF 2011-08nuts*.xls" includes the raw data and a precision study. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. * For PDF versions of these papers see folder \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Data Processing Notes: ---------------------- Transmissivity, PAR and SBE Fluorescence data are nominal and unedited. For details on how the transmissivity calibration parameters were calculated see the document in folder "\cruise_data\documents\transmissivity". SBE DO calibration was done using the method described in the SeaBird Application NOte #64-2. For further processing details see the processing report 2011-08-proc.doc. Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2011\2011-008_Nov2021\Processing\IOS\2011-008-0012. hplc --------------------------------------------------------------------------- Sample_Number 40: HPLC: TChl-a low compared to Turner value Sample_Number 41: HPLC: TChl-a low compared to Turner value Sample_Number 43: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! --------------- ------------ ---- ------ ------------ Pressure dbars 10 -99 () (-0.9039 1) PAR:Reference uE/m^2/sec 10 -99 () (0 1527.282) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- --8--- ---9---- --10-- --11- -12- --13- ---14--- 15 ---16-- --17- 18 --19- 20 --21- 22 --23-- 24 --25-- 26 --27-- -28- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- 52 !Samp Bott Pressu Temperat Trans Fluores PAR PAR: Salinity Oxygen Oxyge Numb Tempe Salinity Fl Oxygen: Oxyge Fl Nitra Fl Silic Fl Phosph Fl Chloro Fl Phaeo- Bott HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le:F re ure: missi cence: Refere :T1:C1 : n: er_o ratur :Bottle ag Dissolv n: ag te_ ag ate ag ate ag phyll: ag Pigmen le:F Chl-c3 Chlide- MgDVP Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb ~ng_ Secondar vity URU: nce Dissol Disso ~bin e: ~o ed Disso ~o plus_ ~t ~i ~p Extrac ~a t: ~ng_ a a fuco fuco : !er Sequ y Seapoin ved: lved: _rec Draw tt lved lv Nitri ri ca ha ted ct Extrac Sequ HP ! ence t SBE SBE ords le ed te te te te ed ted ence LC !---- ---- ------ -------- ----- ------- ------ ------ -------- ------ ----- ---- ----- -------- -- ------- ----- -- ----- -- ----- -- ------ -- ------ -- ------ ---- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 43 16 3.2 8.0069 32.5 5.286 389.2 1959.5 28.6792 7.87 343.8 241 8.0 -99.0000 0 7.881 344.2 0 13.1 0 24.2 0 1.40 0 18.04 3 3.15 16 0.052 0.797 0.128 1.373 0.088 0.184 0.030 7.989 0.000 0.000 0.006 0.000 0.700 0.016 0.057 0.045 0.000 0.011 0.000 11.980 0.200 0.280 12.776 36 42 15 3.8 8.0007 32.8 6.908 365.3 1699.9 28.6765 7.85 342.7 241 8.0 -99.0000 0 7.875 343.9 0 13.3 0 24.4 0 1.38 0 -99.00 0 -99.00 15 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 41 14 9.1 7.9979 33.2 7.907 31.8 1814.4 28.6808 7.83 342.0 241 7.9 -99.0000 0 7.857 343.1 6 13.2 0 24.3 0 1.39 0 18.76 6 1.32 14 0.066 0.809 0.104 1.085 0.090 0.000 0.000 6.332 0.000 0.000 0.011 0.000 0.508 0.005 0.041 0.035 0.000 0.000 0.000 8.712 0.000 0.218 9.522 26 40 13 19.2 7.9951 33.2 7.947 1.0 1890.8 28.6861 7.77 339.3 241 7.9 -99.0000 0 7.809 341.0 0 13.5 0 24.6 0 1.45 0 16.62 3 2.82 13 0.034 0.654 0.106 1.072 0.083 0.000 0.000 6.437 0.000 0.000 0.006 0.000 0.450 0.006 0.031 0.038 0.000 0.009 0.000 9.320 0.000 0.234 9.974 26 39 12 28.9 7.9977 33.9 8.032 0.0 1915.2 28.7299 7.69 335.8 241 7.8 -99.0000 0 7.678 335.3 0 14.4 0 25.9 0 1.52 0 -99.00 0 -99.00 12 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 38 11 39.3 8.0084 34.7 7.732 0.0 1657.1 28.7962 7.52 328.4 241 7.9 -99.0000 0 7.540 329.2 0 15.2 0 27.7 0 1.60 0 -99.00 0 -99.00 11 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 37 10 49.0 8.0038 36.5 6.795 0.0 1553.2 28.9366 7.17 313.1 241 7.8 -99.0000 0 7.119 310.8 0 17.3 0 31.2 0 1.91 0 -99.00 0 -99.00 10 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 36 9 73.8 8.2926 58.2 0.374 0.0 1487.6 29.7918 4.54 198.0 241 8.1 -99.0000 0 4.464 194.8 0 29.9 0 59.6 0 2.55 0 -99.00 0 -99.00 9 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 35 8 98.9 8.4486 59.3 0.167 0.0 1415.8 30.0929 4.26 185.9 241 8.1 -99.0000 0 4.342 189.4 0 30.1 0 58.7 0 2.46 0 -99.00 0 -99.00 8 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 34 7 123.8 8.3143 58.4 0.115 0.0 1289.0 30.2703 4.39 191.3 241 8.1 -99.0000 0 4.427 193.1 0 30.0 0 58.0 0 2.50 0 -99.00 0 -99.00 7 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 33 6 149.3 8.2067 56.9 0.109 0.0 1160.7 30.3294 4.49 195.6 241 7.9 -99.0000 0 4.502 196.3 0 29.6 6 57.4 6 2.48 6 -99.00 0 -99.00 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 32 5 173.5 8.2255 56.4 0.115 0.0 1064.5 30.3801 4.44 193.6 241 8.0 -99.0000 0 4.434 193.4 3 29.7 0 57.8 0 2.41 0 -99.00 0 -99.00 5 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 31 4 199.1 8.3319 55.9 0.098 0.0 1005.0 30.4657 4.25 185.4 241 8.1 -99.0000 0 4.240 184.9 36 29.6 0 58.8 0 2.51 0 -99.00 0 -99.00 4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 30 3 249.5 8.7784 54.3 0.093 0.0 972.9 30.6081 3.72 162.2 241 8.3 -99.0000 0 3.740 163.1 0 30.3 0 62.0 0 2.64 0 -99.00 0 -99.00 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 29 2 298.6 9.0174 54.3 0.087 0.0 977.5 30.6820 3.42 149.3 241 8.3 30.6845 0 3.443 150.1 0 30.0 0 63.4 0 2.63 0 -99.00 0 -99.00 2 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 28 1 350.8 9.2590 49.3 0.093 0.0 994.3 30.7598 3.02 131.5 241 8.6 -99.0000 0 3.071 133.9 0 30.2 0 68.6 0 2.73 0 -99.00 0 -99.00 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0