*2021/11/24 16:55:29.12 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2010/08/06 08:36:33.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 24 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : C40A9E8F NUMBER OF CHANNELS : 49 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 1125 1148 2 Bottle_Number n/a 1 24 3 Bottle:Firing_Sequence n/a 1 24 4 Pressure decibar 3.2 1761.3 5 Temperature:Secondary 'deg C (ITS90)' 2.2179 11.252 6 Transmissivity %/metre 15.6 63.2 7 Fluorescence:URU:Seapoint mg/m^3 0.49E-01 9.987 8 Salinity:T1:C1 PSS-78 32.0998 34.5573 9 Oxygen:Dissolved:SBE mL/L 0.23 7.24 10 Oxygen:Dissolved:SBE umol/kg 9.9 315.5 11 Number_of_bin_records n/a 121 121 12 Salinity:Bottle PSS-78 32.1044 34.5022 13 Flag:Salinity:Bottle n/a 14 Oxygen:Dissolved mL/L 0.218 7.29 15 Flag:Oxygen:Dissolved n/a 16 Nitrate_plus_Nitrite umol/L 2.6 45.9 17 Flag:Nitrate_plus_Nitrite n/a 18 Silicate umol/L 15.6 169.3 19 Flag:Silicate n/a 20 Phosphate umol/L 0.63 3.31 21 Flag:Phosphate n/a 22 Chlorophyll:Extracted mg/m^3 0.28 18.25 23 Flag:Chlorophyll:Extracted n/a 24 Phaeo-Pigment:Extracted mg/m^3 0.39 5.19 25 HPLC:Chl-c3 mg/m^3 0 0.348 26 HPLC:Chlide-a mg/m^3 0 0.809 27 HPLC:MgDVP mg/m^3 0 0.141 28 HPLC:Chl-c2 mg/m^3 0.16E-01 2.033 29 HPLC:Me-chlide mg/m^3 0 2.077 30 HPLC:Peri mg/m^3 0 0.292 31 HPLC:Pheide-a mg/m^3 0 1.051 32 HPLC:But-fuco mg/m^3 0 0.7E-02 33 HPLC:Fuco mg/m^3 0.91E-01 5.237 34 HPLC:Neo mg/m^3 0 0.41E-01 35 HPLC:Pras mg/m^3 0 0.26E-01 36 HPLC:Viola mg/m^3 0 0.32E-01 37 HPLC:Hex-fuco mg/m^3 0 0.94E-01 38 HPLC:Diadino mg/m^3 0.6E-02 0.492 39 HPLC:Allo mg/m^3 0 0.111 40 HPLC:Diato mg/m^3 0 0.18E-01 41 HPLC:Zea mg/m^3 0 0.38E-01 42 HPLC:Lut mg/m^3 0 0.9E-02 43 HPLC:Chl-b mg/m^3 0 0.157 44 HPLC:DVChl-a mg/m^3 0 0 45 HPLC:Chl-a mg/m^3 0.155 7.781 46 HPLC:Phe mg/m^3 0 0.219 47 HPLC:B-Car mg/m^3 0.4E-02 0.114 48 HPLC:TChl-a mg/m^3 0.155 10.667 49 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 9 F ' ' 4 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 5 I I 0 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 8 F R4 3 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 6 F R4 1 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 6 F R4 1 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 7 F R4 2 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2010-36 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Ocean Acidification SCIENTIST : Ianson D. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : LG7 EVENT NUMBER : 129 LATITUDE : 48 59.36000 N ! (deg min) LONGITUDE : 127 7.22000 W ! (deg min) WATER DEPTH : 1750 ALTIMETER (M) : 13.94 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.18C $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1 2011/01/03 15:44:12 2904 2904 CLEAN 5.0.2 2011/01/03 15:45:28 ? ? ADDSAMP 3.4 2011/01/10 13:11:23 2904 2904 BINAVE 4.1.1 2011/01/10 13:12:37 2904 24 MERGE 3.4 2011/01/14 15:31:34 24 24 CLEAN 5.0.2 2011/01/14 17:23:08 ? ? CALIB 11.7 2011/02/07 09:25:05 24 24 SORT 3.5 2011/02/08 13:09:35 24 24 REMOVECH 8.0 2011/02/08 17:07:38 24 24 REMOVECH 8.0 2011/02/08 17:10:29 24 24 CHGUNITS 3.0 2011/02/08 17:18:37 24 24 REORDER 1.3 2011/02/09 14:56:47 ? ? HDREDIT2 2.5.1 2011/02/09 14:56:56 ? ? CALIB 11.7 2013/07/11 15:10:01 24 24 HDREDIT2 2.6 2013/07/11 15:17:37 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:35:09 24 24 CHANGE_FLAGS 2.0 2014/11/20 09:57:25 24 24 SORT 3.6 2020/11/30 13:15:10 24 24 REORDER 1.3.1 2021/11/24 16:53:54 ? ? MERGE 3.5.1 2021/11/24 16:54:18 24 24 SORT 3.6 2021/11/24 16:54:26 24 24 CLEAN 5.2.4 2021/11/24 16:54:36 24 24 HDREDIT2 3.2 2021/11/24 16:55:06 ? ? HDREDIT2 3.2 2021/11/24 16:55:29 ? ? $END $REMARKS -CLEAN functions: 2011/01/03 15:44:50 20 Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\addsamp3.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2011/01/14 15:31:30 2011/01/14 15:31:30 2011/01/14 15:31: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Flag:Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0129.samavg Secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0129.mrgx Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0129.mrgx -------------------------------------------------------------------- -SORT parameters: 2011/01/14 15:31:12 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2011/01/14 17:23:07 20 Remove Sea-Bird comments from the header. -CALIB parameters: 2011/02/07 09:25:05 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data_Processing\2010-36\Processing\doc\2010-36-recal-fit2.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Oxygen:Dissolved:SBE mL/L 10 0.2383000E+00 0.1034700E+01 -SORT parameters: 2011/02/08 13:09:35 Sorted in ascending order of channel Pressure -REMOVECH 2011/02/08 17:07:38 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] pH:SBE [pH Units] Status:Pump Altimeter [metres] Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -REMOVECH 2011/02/08 17:10:29 No channels were removed. -CHANGE units: 'Oxygen:Dissolved:SBE [mL/L]' changed from mL/L to umol/kg -HEADER EDITS: 2011/02/09 14:56:56 Applied edit header: Q:\Cruise_Data_Processing\2010-36\Processing\doc\2010-36-bot-hdr.txt Channel 10: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 4: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 9: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 6: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 16: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 17: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 7: Oxygen:Dissolved:SBE [umol/kg] Format: F6.2 ==> F6.1 -CALIB parameters: 2013/07/11 15:10:01 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Nitrate_plus_Nitrite umol/L 10 0.1709000E+00 0.9812000E+00 23 Phosphate umol/L 10 0.4530000E-01 0.1011500E+01 -HEADER EDITS: 2013/07/11 15:17:37 Applied edit header: C:\DATA\Nutrients\doc\headedit-correction.txt -SORT parameters: 2020/11/30 13:15:10 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/24 16:54:18 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036_Nov2021\Processing\ IOS\2010-036-0129.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036_Nov2021\Processing\ IOS\2010-036-0129.hplc -SORT parameters: 2021/11/24 16:54:26 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/24 16:54:34 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/24 16:55:06 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 25: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/24 16:55:29 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036-bot-hdr2.txt $END *COMMENTS Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0129.mrgx -------------------------------------------------------------------- Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0129.chl ------------------------------------------------------------------- Sample_Number 1145: CHL: variability of reps >15% CV Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0129.nut ------------------------------------------------------------------- Sample_Number 1140: NUTS: sampled out of order but corrected Analysis methods: - ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone for 24 hours in the lab immediately following the cruise and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen 1978). For details including information on precision see file 2010-36CHL.xls HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~7-10 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2010-036_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). The titration system consists of a Brinkmann 665 Dosimat, a UV light source and a UV detector with a 365nm filter custom built by Scripps Institution of Oceanography. For details including details on precision, see document 2010-36oxy.xls. Salinity samples were collected in glass bottles and analyzed at sea using a Guildline Portasal (#58879) or at IOS on a Guildline model 8400B Autosal standardized with IAPSO standard seawater. See file 2010-36-salinity.xls for data for information on precision see 2010-36-salinity-duplicates.csv. Nutrient samples were collected in plastic tubes. Most samples were analyzed at sea on an Astoria-Pacific analyzer following methods described in Barwell-Clarke and Whitney (1996). Samples from Event #225 at station JF2 were frozen and analyzed after 2 weeks. For full data and details on nutrient duplicates and precision see file QF2010-36nuts.xls. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. Culberson, C. H., and R. T. Williams. 1991. A comparison of methods for the determination of dissolved oxygen in seawater. Report No. WHPO 91-2. WOCE Hydrographic Programme Office. Woods Hole Oceanographic Institution, Woods Hole, Mass. 3. Barwell-Clarke, J. and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 4. Holm-Hansen, O. (1978). Chlorophyll a determination: improvement in methodology. Oikos 30:438-447. ----------------------------------------------------------------------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. The pH:SBE channel was removed from the files because sensor performance and recalibration are being studied and are not yet considered ready for the archive. The SBE Dissolved Oxygen sensor behaved poorly for all or part of some casts with concentration values offset by approximately 0.24mL/L from the normal looking casts. Data were recalibrated by applying an offset where appropriate before applying a general recalibration. The DO data must be considered of lower quality than usual. July 2013: A correction has been applied to Nitrate_plus_Nitrite and Phosphate data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the following report: Corrections to Nitrate and Phosphate for Deep-Water Cruises from 2009 to 2012.pdf which is stored in folder: \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036_Nov2021\Processing\IOS\2010-036-0129. hplc --------------------------------------------------------------------------- Sample_Number 1146: HPLC: >10%CV for TChl-a 8. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- -------- ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.2383 1.0347) Nitrate_plus_Nitrite umol/L 10 -99 () (0.1709 0.9812) Phosphate umol/L 10 -99 () (0.453E-01 1.0115) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8---- --9--- --10- -11- ---12--- 13 ---14-- 15 --16- 17 --18- 19 --20-- 21 --22-- 23 --24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- 49 !Samp Bott Bott Pressu Temperat Trans Fluores Salinity Oxygen Oxyge Numb Salinity Fl Oxygen: Fl Nitra Fl Silic Fl Phosph Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: :T1:C1 : n: er_o :Bottle ag Dissolv ag te_ ag ate ag ate ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: Dissol Disso ~bin ~o ed ~o plus_ ~t ~i ~p Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin ved: lved: _rec tt lv Nitri ri ca ha ted ct Extrac HP ! ence t SBE SBE ords le ed te te te te ed ted LC !---- ---- ---- ------ -------- ----- ------- -------- ------ ----- ---- -------- -- ------- -- ----- -- ----- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 1144 20 24 3.2 11.2520 15.6 9.978 32.1004 7.24 315.5 121 -99.0000 0 7.290 0 2.7 0 15.8 0 0.64 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1148 24 23 6.2 11.2354 15.9 9.748 32.1026 7.22 314.5 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1143 19 21 6.3 11.2512 15.7 9.835 32.0998 7.21 314.3 121 32.1044 0 7.278 0 2.6 0 15.6 0 0.63 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1147 23 22 6.3 11.2277 15.7 9.987 32.1035 7.19 313.2 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 18.25 6 5.19 0.348 0.809 0.141 2.033 2.077 0.292 1.051 0.000 5.237 0.041 0.026 0.032 0.094 0.492 0.111 0.018 0.038 0.009 0.157 0.000 7.781 0.219 0.114 10.667 6 1142 18 20 21.1 8.7447 61.6 0.151 32.3683 5.49 239.1 121 -99.0000 0 5.489 0 12.9 0 18.5 0 1.28 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1146 22 19 30.9 8.1824 60.7 0.192 32.5066 4.55 198.0 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 0.51 6 0.47 0.004 0.000 0.000 0.037 0.000 0.015 0.069 0.007 0.186 0.000 0.000 0.000 0.000 0.011 0.002 0.000 0.002 0.000 0.000 0.000 0.302 0.000 0.007 0.302 36 1141 17 18 31.0 8.1809 60.8 0.205 32.5070 4.56 198.5 121 -99.0000 0 4.608 0 17.5 0 22.1 0 1.49 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1145 21 17 41.2 7.7748 61.5 0.132 32.7867 4.20 182.7 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 0.28 36 0.39 0.000 0.000 0.000 0.016 0.000 0.000 0.000 0.000 0.091 0.000 0.000 0.000 0.000 0.006 0.000 0.008 0.000 0.000 0.000 0.000 0.155 0.000 0.004 0.155 6 1140 16 16 51.3 7.3267 62.7 0.079 32.8920 5.21 226.8 121 -99.0000 0 5.259 6 18.8 6 27.9 6 1.62 6 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1139 15 15 71.6 6.9654 62.3 0.079 33.3557 4.24 184.7 121 -99.0000 0 4.291 0 23.9 0 31.1 0 1.84 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1138 14 14 81.3 6.7320 62.6 0.077 33.4525 4.42 192.4 121 -99.0000 0 4.413 0 24.1 0 31.7 0 1.82 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1137 13 13 91.2 6.6233 63.1 0.065 33.5969 4.29 186.5 121 -99.0000 0 4.348 0 25.0 0 33.5 0 1.85 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1136 12 12 101.3 6.9958 62.7 0.060 33.7621 3.13 136.2 121 -99.0000 0 3.132 0 29.5 0 39.3 0 2.15 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1135 11 11 126.4 6.8005 62.7 0.062 33.8404 2.98 129.6 121 -99.0000 0 2.989 0 30.6 0 42.6 0 2.20 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1134 10 10 152.0 6.6337 62.4 0.061 33.8806 2.55 110.8 121 -99.0000 0 2.551 0 32.5 0 46.9 0 2.34 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1133 9 9 200.9 6.3544 62.3 0.060 33.9164 2.11 91.7 121 -99.0000 0 2.114 0 34.9 0 53.3 0 2.49 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1132 8 8 300.9 5.7225 62.4 0.060 33.9910 1.38 60.0 121 -99.0000 0 1.380 0 39.1 0 66.1 0 2.79 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1131 7 7 401.3 5.2401 63.2 0.055 34.0600 0.87 37.9 121 -99.0000 0 0.860 0 42.1 0 77.7 0 3.00 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1130 6 6 501.1 4.5995 63.2 0.056 34.1126 0.57 24.7 121 -99.0000 0 0.553 0 44.0 0 92.1 0 3.12 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1129 5 5 601.7 4.3699 63.0 0.054 34.1821 0.39 16.9 121 -99.0000 0 0.379 0 44.8 6 101.0 6 3.20 6 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1128 4 4 801.4 3.9133 62.8 0.052 34.3183 0.23 9.9 121 -99.0000 0 0.218 6 45.3 0 118.5 0 3.30 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1127 3 3 1000.9 3.4306 62.6 0.051 34.4018 0.27 11.6 121 -99.0000 0 0.258 0 45.9 0 133.4 0 3.31 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1126 2 2 1400.9 2.6635 62.2 0.049 34.5025 0.57 24.7 121 34.5022 0 0.579 0 45.3 0 156.1 0 3.24 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1125 1 1 1761.3 2.2179 62.0 0.049 34.5573 0.96 41.8 121 -99.0000 0 0.999 0 44.8 0 169.3 0 3.15 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0