*2021/11/24 16:55:28.08 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2010/08/02 04:34:04.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 24 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 61BE51B1 NUMBER OF CHANNELS : 49 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 787 810 2 Bottle_Number n/a 1 24 3 Bottle:Firing_Sequence n/a 1 24 4 Pressure decibar 3.4 1876.2 5 Temperature:Secondary 'deg C (ITS90)' 2.0109 12.0435 6 Transmissivity %/metre 21.4 63.5 7 Fluorescence:URU:Seapoint mg/m^3 0.4E-01 6.704 8 Salinity:T1:C1 PSS-78 31.6103 34.5775 9 Oxygen:Dissolved:SBE mL/L 0.28 6.89 10 Oxygen:Dissolved:SBE umol/kg 12 300.4 11 Number_of_bin_records n/a 120 121 12 Salinity:Bottle PSS-78 31.6223 34.4842 13 Flag:Salinity:Bottle n/a 14 Oxygen:Dissolved mL/L 0.269 6.873 15 Flag:Oxygen:Dissolved n/a 16 Nitrate_plus_Nitrite umol/L 1.6 46.2 17 Flag:Nitrate_plus_Nitrite n/a 18 Silicate umol/L 7.2 175 19 Flag:Silicate n/a 20 Phosphate umol/L 0.54 3.28 21 Flag:Phosphate n/a 22 Chlorophyll:Extracted mg/m^3 5.44 8.85 23 Flag:Chlorophyll:Extracted n/a 24 Phaeo-Pigment:Extracted mg/m^3 1.37 1.52 25 HPLC:Chl-c3 mg/m^3 0.135 0.151 26 HPLC:Chlide-a mg/m^3 0.2E-01 0.173 27 HPLC:MgDVP mg/m^3 0.23E-01 0.64E-01 28 HPLC:Chl-c2 mg/m^3 0.634 1.07 29 HPLC:Me-chlide mg/m^3 0.19E-01 0.163 30 HPLC:Peri mg/m^3 0.72E-01 0.201 31 HPLC:Pheide-a mg/m^3 0 0.141 32 HPLC:But-fuco mg/m^3 0.25E-01 0.32E-01 33 HPLC:Fuco mg/m^3 2.568 4.214 34 HPLC:Neo mg/m^3 0 0.16E-01 35 HPLC:Pras mg/m^3 0 0.24E-01 36 HPLC:Viola mg/m^3 0 0.21E-01 37 HPLC:Hex-fuco mg/m^3 0.24E-01 0.124 38 HPLC:Diadino mg/m^3 0.142 0.464 39 HPLC:Allo mg/m^3 0.15E-01 0.75E-01 40 HPLC:Diato mg/m^3 0.18E-01 0.56E-01 41 HPLC:Zea mg/m^3 0.27E-01 0.54E-01 42 HPLC:Lut mg/m^3 0 0.9E-02 43 HPLC:Chl-b mg/m^3 0.37E-01 0.127 44 HPLC:DVChl-a mg/m^3 0 0 45 HPLC:Chl-a mg/m^3 4.659 7.906 46 HPLC:Phe mg/m^3 0.66E-01 0.341 47 HPLC:B-Car mg/m^3 0.124 0.279 48 HPLC:TChl-a mg/m^3 4.698 8.241 49 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 9 F ' ' 4 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 5 I I 0 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 8 F R4 3 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 6 F R4 1 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 6 F R4 1 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 7 F R4 2 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2010-36 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Ocean Acidification SCIENTIST : Ianson D. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : OACS5 EVENT NUMBER : 89 LATITUDE : 50 34.50000 N ! (deg min) LONGITUDE : 129 0.08000 W ! (deg min) WATER DEPTH : 1859 ALTIMETER (M) : 9.3 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item $REMARKS SOFTWARE VERSION SEASAVE V 7.18C $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1 2011/01/03 15:44:00 1935 1935 CLEAN 5.0.2 2011/01/03 15:45:17 ? ? JOIN 2.4.2 2011/01/05 13:42:32 2903 2903 ADDSAMP 3.4 2011/01/10 13:11:12 2903 2903 BINAVE 4.1.1 2011/01/10 13:12:31 2903 24 MERGE 3.4 2011/01/19 12:52:17 24 24 CLEAN 5.0.2 2011/01/19 12:52:19 ? ? CALIB 11.7 2011/02/07 09:25:04 24 24 SORT 3.5 2011/02/08 13:09:34 24 24 REMOVECH 8.0 2011/02/08 17:07:38 24 24 REMOVECH 8.0 2011/02/08 17:10:29 24 24 CHGUNITS 3.0 2011/02/08 17:18:37 24 24 REORDER 1.3 2011/02/09 14:56:47 ? ? HDREDIT2 2.5.1 2011/02/09 14:56:55 ? ? CALIB 11.7 2013/07/11 15:09:59 24 24 HDREDIT2 2.6 2013/07/11 15:17:36 ? ? CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:35:09 24 24 CHANGE_FLAGS 2.0 2014/11/20 09:57:24 24 24 SORT 3.6 2020/11/30 13:15:09 24 24 REORDER 1.3.1 2021/11/24 16:53:53 ? ? MERGE 3.5.1 2021/11/24 16:54:17 24 24 SORT 3.6 2021/11/24 16:54:26 24 24 CLEAN 5.2.4 2021/11/24 16:54:35 24 24 HDREDIT2 3.2 2021/11/24 16:55:05 ? ? HDREDIT2 3.2 2021/11/24 16:55:28 ? ? $END $REMARKS -CLEAN functions: 2011/01/03 15:44:50 20 Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\addsamp3.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2011/01/19 12:52:17 Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Flag:Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate, Ammonium, Flag:Ammonium, pH, Flag:pH, Dimethyl_Sulphide, Flag:Dimethyl_Sulphide Primary file : Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0089.samavg Secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0089.mrgx Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0089.mrgx -------------------------------------------------------------------- -SORT parameters: 2011/01/19 12:33:54 Sorted in ascending order of channel Bottle_Number -CLEAN functions: 2011/01/19 12:52:19 Remove Sea-Bird comments from the header. -CALIB parameters: 2011/02/07 09:25:04 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data_Processing\2010-36\Processing\doc\2010-36-recal-fit2.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Oxygen:Dissolved:SBE mL/L 10 0.2383000E+00 0.1034700E+01 -SORT parameters: 2011/02/08 13:09:34 Sorted in ascending order of channel Pressure -REMOVECH 2011/02/08 17:07:38 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] pH:SBE [pH Units] Status:Pump Altimeter [metres] Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -REMOVECH 2011/02/08 17:10:29 No channels were removed. -CHANGE units: 'Oxygen:Dissolved:SBE [mL/L]' changed from mL/L to umol/kg -HEADER EDITS: 2011/02/09 14:56:55 Applied edit header: Q:\Cruise_Data_Processing\2010-36\Processing\doc\2010-36-bot-hdr.txt Channel 10: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 4: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 9: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 6: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 16: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 17: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 7: Oxygen:Dissolved:SBE [umol/kg] Format: F6.2 ==> F6.1 -CALIB parameters: 2013/07/11 15:09:59 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = C:\DATA\Nutrients\doc\Nutrients-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 19 Nitrate_plus_Nitrite umol/L 10 0.1709000E+00 0.9812000E+00 23 Phosphate umol/L 10 0.4530000E-01 0.1011500E+01 -HEADER EDITS: 2013/07/11 15:17:36 Applied edit header: C:\DATA\Nutrients\doc\headedit-correction.txt -SORT parameters: 2020/11/30 13:15:09 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/24 16:54:17 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036_Nov2021\Processing\ IOS\2010-036-0089.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036_Nov2021\Processing\ IOS\2010-036-0089.hplc -SORT parameters: 2021/11/24 16:54:26 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/24 16:54:34 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/24 16:55:05 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 25: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/24 16:55:28 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036-bot-hdr2.txt $END *COMMENTS Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0089.mrgx -------------------------------------------------------------------- Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0089.sal ------------------------------------------------------------------- Sample 789: SAL: Originally named #788, but that is outlier in COMPARE; looks like really came from Niskin #3 so renamed #789. Comments from secondary file: Q:\Cruise_Data_Processing\2010-36\Processing\hydro\2010-36-0089.add ------------------------------------------------------------------- Sample_Number 790: OXY: problem with titration Analysis methods: - ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone for 24 hours in the lab immediately following the cruise and analyzed on a Turner 10AU flourometer calibrated with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen 1978). For details including information on precision see file 2010-36CHL.xls HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~7-10 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide For further information see file QF 2010-036_HPLC*.xlsx. Oxygen samples were analyzed at sea using an automated Winkler titration system with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). The titration system consists of a Brinkmann 665 Dosimat, a UV light source and a UV detector with a 365nm filter custom built by Scripps Institution of Oceanography. For details including details on precision, see document 2010-36oxy.xls. Salinity samples were collected in glass bottles and analyzed at sea using a Guildline Portasal (#58879) or at IOS on a Guildline model 8400B Autosal standardized with IAPSO standard seawater. See file 2010-36-salinity.xls for data for information on precision see 2010-36-salinity-duplicates.csv. Nutrient samples were collected in plastic tubes. Most samples were analyzed at sea on an Astoria-Pacific analyzer following methods described in Barwell-Clarke and Whitney (1996). Samples from Event #225 at station JF2 were frozen and analyzed after 2 weeks. For full data and details on nutrient duplicates and precision see file QF2010-36nuts.xls. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. Culberson, C. H., and R. T. Williams. 1991. A comparison of methods for the determination of dissolved oxygen in seawater. Report No. WHPO 91-2. WOCE Hydrographic Programme Office. Woods Hole Oceanographic Institution, Woods Hole, Mass. 3. Barwell-Clarke, J. and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 4. Holm-Hansen, O. (1978). Chlorophyll a determination: improvement in methodology. Oikos 30:438-447. ----------------------------------------------------------------------------------- Transmissivity, fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. The pH:SBE channel was removed from the files because sensor performance and recalibration are being studied and are not yet considered ready for the archive. The SBE Dissolved Oxygen sensor behaved poorly for all or part of some casts with concentration values offset by approximately 0.24mL/L from the normal looking casts. Data were recalibrated by applying an offset where appropriate before applying a general recalibration. The DO data must be considered of lower quality than usual. July 2013: A correction has been applied to Nitrate_plus_Nitrite and Phosphate data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the following report: Corrections to Nitrate and Phosphate for Deep-Water Cruises from 2009 to 2012.pdf which is stored in folder: \\Cruise_Data\DOCUMENTS\Analysis Reference Papers Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-036_Nov2021\Processing\IOS\2010-036-0089. hplc --------------------------------------------------------------------------- Sample_Number 808: HPLC: one replicate thawed but values appear unaffected 8. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- -------- ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.2383 1.0347) Nitrate_plus_Nitrite umol/L 10 -99 () (0.1709 0.9812) Phosphate umol/L 10 -99 () (0.453E-01 1.0115) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- ---8---- --9--- --10- -11- ---12--- 13 ---14-- 15 --16- 17 --18- 19 --20-- 21 --22-- 23 --24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- 49 !Samp Bott Bott Pressu Temperat Trans Fluores Salinity Oxygen Oxyge Numb Salinity Fl Oxygen: Fl Nitra Fl Silic Fl Phosph Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: :T1:C1 : n: er_o :Bottle ag Dissolv ag te_ ag ate ag ate ag phyll: ag Pigmen Chl-c3 Chlide- MgDVP Chl-c2 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: Dissol Disso ~bin ~o ed ~o plus_ ~t ~i ~p Extrac ~a t: a chlide a fuco fuco : !er er Sequ y Seapoin ved: lved: _rec tt lv Nitri ri ca ha ted ct Extrac HP ! ence t SBE SBE ords le ed te te te te ed ted LC !---- ---- ---- ------ -------- ----- ------- -------- ------ ----- ---- -------- -- ------- -- ----- -- ----- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 807 21 24 3.4 12.0435 21.4 6.704 31.6103 6.89 300.4 121 -99.0000 0 6.866 0 1.6 0 7.2 0 0.55 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 806 20 23 6.3 12.0321 21.4 6.546 31.6129 6.88 299.7 121 31.6223 0 6.873 0 1.6 0 7.3 0 0.54 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 810 24 22 6.3 12.0254 21.5 6.439 31.6144 6.88 300.0 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 8.85 6 1.52 0.139 0.173 0.059 1.070 0.162 0.195 0.000 0.029 4.214 0.015 0.007 0.018 0.106 0.464 0.075 0.056 0.053 0.009 0.117 0.000 7.906 0.341 0.279 8.241 6 809 23 21 11.4 11.9312 22.2 6.574 31.6358 6.79 296.1 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 8.82 6 1.51 0.135 0.166 0.064 1.023 0.163 0.201 0.000 0.032 3.998 0.016 0.024 0.021 0.124 0.437 0.072 0.035 0.054 0.005 0.127 0.000 7.511 0.194 0.247 7.841 6 805 19 19 21.7 11.0197 39.3 2.625 31.7234 6.24 272.1 121 -99.0000 0 6.211 0 6.0 0 14.9 0 0.92 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 808 22 20 21.9 11.0774 39.8 2.919 31.7111 6.29 274.0 121 -99.0000 0 -99.000 0 -99.0 0 -99.0 0 -99.00 0 5.44 6 1.37 0.151 0.020 0.023 0.634 0.019 0.072 0.141 0.025 2.568 0.000 0.000 0.000 0.024 0.142 0.015 0.018 0.027 0.000 0.037 0.000 4.659 0.066 0.124 4.698 26 804 18 18 30.9 10.0145 51.6 1.187 31.9853 5.50 239.8 121 -99.0000 0 5.510 0 11.1 0 23.0 0 1.29 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 803 17 17 71.0 7.1042 62.8 0.061 33.2641 4.03 175.4 120 33.2613 0 4.019 0 24.7 0 34.4 0 1.90 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 802 16 16 71.3 7.1293 62.7 0.068 33.2537 4.05 176.1 121 33.2729 0 4.024 0 24.8 0 34.4 0 1.89 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 801 15 15 91.3 6.8076 63.0 0.050 33.4471 3.55 154.4 121 -99.0000 0 3.532 6 28.4 0 40.6 0 2.06 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 800 14 14 101.8 6.7495 63.0 0.049 33.4938 3.48 151.5 121 -99.0000 0 3.466 0 28.5 0 41.3 0 2.07 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 799 13 13 126.5 6.4250 63.5 0.049 33.6642 4.36 189.4 121 -99.0000 0 4.346 0 25.7 0 35.9 0 1.85 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 798 12 12 151.6 6.4521 63.4 0.049 33.7303 3.96 172.2 121 -99.0000 0 3.984 0 26.6 0 39.1 0 1.96 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 797 11 11 201.1 5.9853 63.3 0.050 33.8236 3.46 150.6 121 -99.0000 0 3.502 0 29.6 0 47.6 0 2.16 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 796 10 10 301.3 5.5737 63.2 0.049 33.9771 1.49 64.6 121 -99.0000 0 1.481 0 38.1 0 67.9 0 2.77 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 795 9 9 400.8 5.0910 63.3 0.049 34.0435 0.96 41.9 121 -99.0000 0 0.964 0 41.1 0 80.2 0 2.97 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 794 8 8 500.7 4.7582 62.7 0.049 34.1134 0.64 27.8 121 -99.0000 0 0.629 0 43.0 0 91.3 0 3.10 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 793 7 7 601.3 4.4704 62.4 0.049 34.1738 0.45 19.6 121 -99.0000 0 0.435 0 44.4 0 100.6 0 3.17 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 792 6 6 700.8 4.1924 63.2 0.049 34.2318 0.32 13.9 121 -99.0000 0 0.304 0 45.0 6 109.8 6 3.23 6 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 791 5 5 801.6 3.9393 62.5 0.048 34.2901 0.28 12.0 121 -99.0000 0 0.269 0 45.7 0 118.1 0 3.25 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 790 4 4 1001.7 3.4819 62.4 0.049 34.3762 0.28 12.0 121 -99.0000 0 -99.000 5 46.1 0 132.9 0 3.28 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 789 3 3 1401.1 2.6693 62.5 0.045 34.4847 0.51 22.4 121 34.4842 3 0.542 0 46.2 0 156.6 0 3.20 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 788 2 2 1800.9 2.0726 61.6 0.040 34.5668 1.07 46.6 121 -99.0000 0 1.153 0 44.0 0 173.8 0 3.08 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 787 1 1 1876.2 2.0109 61.5 0.042 34.5775 1.15 50.0 121 -99.0000 0 1.198 6 43.6 0 175.0 0 3.06 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0