*2021/11/24 16:48:51.38 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2010/06/25 08:11:12.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 35A658CD NUMBER OF CHANNELS : 38 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 125 125 2 Bottle_Number n/a 1 1 3 Bottle:Firing_Sequence n/a 1 1 4 Pressure decibar 6.2 6.2 5 Temperature:Secondary 'deg C (ITS90)' 11.0325 11.0325 6 Transmissivity %/metre 49.4 49.4 7 Fluorescence:URU:Seapoint mg/m^3 0.176 0.176 8 PAR:Reference uE/m^2/sec 3.6 3.6 9 Salinity:T1:C1 PSS-78 28.9399 28.9399 10 Oxygen:Dissolved:SBE mL/L 5.29 5.29 11 Oxygen:Dissolved:SBE umol/kg 231.2 231.2 12 Number_of_bin_records n/a 241 241 13 Chlorophyll:Extracted mg/m^3 0.74 0.74 14 Flag:Chlorophyll:Extracted n/a 15 Phaeo-Pigment:Extracted mg/m^3 0.53 0.53 16 HPLC:Chl-c3 mg/m^3 0 0 17 HPLC:Chlide-a mg/m^3 0 0 18 HPLC:Chl-c2 mg/m^3 0.137 0.137 19 HPLC:Peri mg/m^3 0.23E-01 0.23E-01 20 HPLC:Pheide-a mg/m^3 0 0 21 HPLC:But-fuco mg/m^3 0.136 0.136 22 HPLC:Fuco mg/m^3 0.139 0.139 23 HPLC:Neo mg/m^3 0.1E-01 0.1E-01 24 HPLC:Pras mg/m^3 0.5E-02 0.5E-02 25 HPLC:Viola mg/m^3 0 0 26 HPLC:Hex-fuco mg/m^3 0.325 0.325 27 HPLC:Diadino mg/m^3 0.62E-01 0.62E-01 28 HPLC:Allo mg/m^3 0.9E-02 0.9E-02 29 HPLC:Diato mg/m^3 0.9E-02 0.9E-02 30 HPLC:Zea mg/m^3 0.13E-01 0.13E-01 31 HPLC:Lut mg/m^3 0.6E-02 0.6E-02 32 HPLC:Chl-b mg/m^3 0.92E-01 0.92E-01 33 HPLC:DVChl-a mg/m^3 0 0 34 HPLC:Chl-a mg/m^3 0.824 0.824 35 HPLC:Phe mg/m^3 0 0 36 HPLC:B-Car mg/m^3 0.11E-01 0.11E-01 37 HPLC:TChl-a mg/m^3 0.824 0.824 38 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 6 F ' ' 1 7 -99 ' ' 8 F ' ' 3 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 7 F R4 2 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 7 F R4 2 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2010-20 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : SoG/JdF Water Properties Survey SCIENTIST : Chandler P. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : 44 EVENT NUMBER : 36 LATITUDE : 48 56.81000 N ! (deg min) LONGITUDE : 123 5.96000 W ! (deg min) WATER DEPTH : 122 ALTIMETER (M) : 7.37 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 $REMARKS SOFTWARE VERSION SEASAVE V 7.18C $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1 2011/04/20 16:56:37 482 482 ADDSAMP 3.5 2011/04/23 17:05:03 482 482 BINAVE 4.1.1 2011/04/23 17:06:54 482 2 MERGE 3.4 2011/04/27 16:55:18 2 2 CLEAN 5.0.2 2011/04/27 16:55:22 ? ? CALIB 11.7 2011/04/27 16:55:28 2 2 SORT 3.5 2011/04/27 17:26:19 2 2 REMOVECH 8.0 2011/04/30 15:43:13 2 2 CHGUNITS 3.0 2011/04/30 15:43:55 2 2 REORDER 1.3 2011/04/30 15:44:23 ? ? HDREDIT2 2.5.1 2011/04/30 15:44:28 ? ? CHANGE_FLAGS 1.0 2013/07/29 14:31:38 2 2 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:35:01 2 2 CHANGE_FLAGS 2.0 2014/11/20 09:57:16 2 2 SORT 3.6 2020/12/14 13:38:58 2 2 REORDER 1.3.1 2021/11/24 16:47:42 ? ? MERGE 3.5.1 2021/11/24 16:47:51 2 1 SORT 3.6 2021/11/24 16:48:01 1 1 CLEAN 5.2.4 2021/11/24 16:48:08 1 1 HDREDIT2 3.2 2021/11/24 16:48:34 ? ? HDREDIT2 3.2 2021/11/24 16:48:51 ? ? $END $REMARKS -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data_Processing\2010-20\Processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2011/04/27 16:55:18 2011/04/27 16:55:18 2011/04/27 16:55: Merge Channel: Bottle_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Phaeo-Pigment:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate Primary file : Q:\Cruise_Data_Processing\2010-20\Processing\hydro\2010-20-0036.samavg Secondary file: Q:\Cruise_Data_Processing\2010-20\Processing\hydro\2010-20-0036.mrgcln1 -CLEAN functions: 2011/04/27 16:55:21 20 Remove Sea-Bird comments from the header. -CALIB parameters: 2011/04/27 16:55:28 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data_Processing\2010-20\Processing\doc\2010-20-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 20 Oxygen:Dissolved:SBE mL/L 10 0.2302000E+00 0.1036600E+01 -SORT parameters: 2011/04/27 17:26:19 Sorted in ascending order of channel Pressure [dbars] -REMOVECH 2011/04/30 15:43:13 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] PAR [uE/m^2/sec] pH:SBE [pH Units] Status:Pump Altimeter [metres] Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE [mL/L]' changed from mL/L to umol/kg -HEADER EDITS: 2011/04/30 15:44:28 Applied edit header: Q:\Cruise_Data_Processing\2010-20\Processing\doc\2010-20-bot-hdr.txt Channel 11: Bottle:Firing_Sequence [n/a] Name: Bottle_Number ==> Bottle:Firing_Sequence Channel 1: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 4: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 10: Bottle_Number [n/a] Name: Bottle:Position ==> Bottle_Number Channel 7: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 15: Phaeo-Pigment:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 5: PAR:Reference [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 8: Oxygen:Dissolved:SBE [umol/kg] Format: F6.2 ==> F6.1 -SORT parameters: 2020/12/14 13:38:58 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/24 16:47:51 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-020_Nov2021\Processing\ IOS\2010-020-0036.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-020_Nov2021\Processing\ IOS\2010-020-0036.hplc -SORT parameters: 2021/11/24 16:48:01 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/24 16:48:07 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/24 16:48:34 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-020\Processing\Doc\ 2010-020-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 3: Bottle:Firing_Sequence [n/a] Format: I3 ==> I5 Channel 16: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/24 16:48:51 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2010\2010-020\Processing\Doc\ 2010-020-bot-hdr2.txt $END *COMMENTS Analysis methods: - ----------------- Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were analyzed in the lab (5 to 7 days after collection) on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen 1965). For details see worksheet “Total-chl” in file 2010-20_CHL*.xls. Phaeopigment data are provided for information only and no quality flags are assigned to them. HPLC samples were filtered onto 25mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~7 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a and Chlide-a. For further information see file QF 2010-020_HPLC*.xlsx. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965) with modifications by Culberson et al. (1991). Samples were analyzed using an automated titration system consisting of a Brinkman Dosimat (model 665) and a PC 950 Colorimeter. All samples were analyzed at sea. File 2010-20oxy*.xls includes the raw data and duplicate analysis. Salinity samples were collected in glass bottles and analyzed on Guildline Autosal model 8400B, S/N 69086, at IOS. Salinometers are standardized with IAPSO standard seawater. Full results are in file 2010-20-sal*.xls. Nutrient samples were collected in plastic tubes and stored frozen and later analysed at IOS using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). File "QF 2010-20nuts*.xls" includes the raw data and an analysis of duplicates. References: 1. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. ----------------------------------------------------------------------------------- ************************************************************************************ The SBE Dissolved Oxygen sensor behaved oddly for casts 12-41, with data offset from those of the other casts when differences between bottles were plotted against DO values. Similar problems were noted when the same sensor was used during cruises 2010-36 and 2010-14 in July and August 2010. Using an appropriate recalibration brought those casts into reasonable agreement with the other casts. For casts without titrated dissolved oxygen sampling, a decision on how to recalibrate was based on the noise level in the raw oxygen voltage. The casts with the offset all had a higher noise level for all three cruises known to have had this problem. ************************************************************************************ *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.2302 1.0366) $END !-1-- -2-- -3-- --4--- ---5---- --6-- ---7--- --8--- ---9---- --10-- --11- -12- --13-- 14 --15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- 38 !Samp Bott Bott Pressu Temperat Trans Fluores PAR: Salinity Oxygen Oxyge Numb Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re ure: missi cence: Refere :T1:C1 : n: er_o phyll: ag Pigmen Chl-c3 Chlide- Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb ~ng_ Secondar vity URU: nce Dissol Disso ~bin Extrac ~a t: a a fuco fuco : !er er Sequ y Seapoin ved: lved: _rec ted ct Extrac HP ! ence t SBE SBE ords ed ted LC !---- ---- ---- ------ -------- ----- ------- ------ -------- ------ ----- ---- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 125 1 1 6.2 11.0325 49.4 0.176 3.6 28.9399 5.29 231.2 241 0.74 6 0.53 0.000 0.000 0.137 0.023 0.000 0.136 0.139 0.010 0.005 0.000 0.325 0.062 0.009 0.009 0.013 0.006 0.092 0.000 0.824 0.000 0.011 0.824 6