*2023/03/19 10:46:20.04 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2009/11/30 22:28:31.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 9 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 1C01BB46 NUMBER OF CHANNELS : 48 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 1 9 2 Bottle_Number n/a 1 9 3 Pressure decibar 1.6 100.7 4 Temperature:Primary 'deg C (ITS90)' 9.1507 10.0998 5 Transmissivity %/metre 1.9 53 6 Fluorescence:URU:Seapoint mg/m^3 0.82E-01 1.736 7 PAR uE/m^2/sec 0.5 246.9 8 PAR:Reference uE/m^2/sec 307.7 472.8 9 Salinity:T0:C0 PSS-78 23.2951 31.1232 10 Oxygen:Dissolved:SBE mL/L 0.37 6.13 11 Oxygen:Dissolved:SBE umol/kg 29.7 261.5 12 Number_of_bin_records n/a 121 121 13 Salinity:Bottle PSS-78 31.1147 31.1147 14 Flag:Salinity:Bottle n/a 15 Oxygen:Dissolved mL/L 0.682 5.963 16 Flag:Oxygen:Dissolved n/a 17 Nitrate_plus_Nitrite umol/L 24.4 27.9 18 Flag:Nitrate_plus_Nitrite n/a 19 Silicate umol/L 52.7 72.6 20 Silicate:Flag n/a 21 Phosphate umol/L 2.26 3.56 22 Flag:Phosphate n/a 23 Chlorophyll:Extracted mg/m^3 0.8E-01 0.65 24 Flag:Chlorophyll:Extracted n/a 25 Bottle:Position n/a 1 9 26 HPLC:Chl-c3 mg/m^3 0.4E-02 0.4E-02 27 HPLC:Chlide-a mg/m^3 0 0 28 HPLC:Chl-c2 mg/m^3 0.15E-01 0.15E-01 29 HPLC:Peri mg/m^3 0 0 30 HPLC:Pheide-a mg/m^3 0 0 31 HPLC:But-fuco mg/m^3 0.6E-02 0.6E-02 32 HPLC:Fuco mg/m^3 0.99E-01 0.99E-01 33 HPLC:Neo mg/m^3 0.7E-02 0.7E-02 34 HPLC:Pras mg/m^3 0.14E-01 0.14E-01 35 HPLC:Viola mg/m^3 0.7E-02 0.7E-02 36 HPLC:Hex-fuco mg/m^3 0 0 37 HPLC:Diadino mg/m^3 0.5E-02 0.5E-02 38 HPLC:Allo mg/m^3 0.9E-02 0.9E-02 39 HPLC:Diato mg/m^3 0 0 40 HPLC:Zea mg/m^3 0.2E-02 0.2E-02 41 HPLC:Lut mg/m^3 0.1E-02 0.1E-02 42 HPLC:Chl-b mg/m^3 0.66E-01 0.66E-01 43 HPLC:DVChl-a mg/m^3 0 0 44 HPLC:Chl-a mg/m^3 0.293 0.293 45 HPLC:Phe mg/m^3 0 0 46 HPLC:B-Car mg/m^3 0.5E-02 0.5E-02 47 HPLC:TChl-a mg/m^3 0.293 0.293 48 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 9 F R4 4 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 8 F R4 3 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 6 F R4 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 3 NQ C ' ' 25 -99 ' ' 3 I I 0 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2009-64 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : SoG/JdF Water Properties Survey SCIENTIST : Chandler P. PLATFORM : Vector *LOCATION GEOGRAPHIC AREA : SoG/JdF STATION : S1 EVENT NUMBER : 1 LATITUDE : 48 39.41000 N ! (deg min) LONGITUDE : 123 29.91000 W ! (deg min) WATER DEPTH : 172 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0550 $REMARKS SOFTWARE VERSION SEASAVE V 7.18 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.1 2010/05/21 14:17:28 1089 1089 CLEAN 5.0.2 2010/05/21 14:19:55 ? ? ADDSAMP 3.4 2010/05/23 16:54:25 1089 1089 BINAVE 4.1.1 2010/05/23 16:54:52 1089 9 MERGE 3.3 2010/06/23 10:40:42 9 9 CLEAN 5.0.2 2010/06/23 10:40:49 ? ? CALIB 11.7 2010/06/23 10:46:22 9 9 SORT 3.5 2010/06/23 11:16:44 9 9 REMOVECH 8.0 2010/06/23 11:18:59 9 9 CHGUNITS 3.0 2010/06/23 11:22:52 9 9 REORDER 1.2.1 2010/06/23 11:31:08 ? ? HDREDIT2 2.5.1 2010/06/23 11:44:39 ? ? CALIB 11.7 2010/10/18 11:13:02 9 9 HDREDIT2 2.5.1 2010/10/18 11:14:38 ? ? CHANGE_FLAGS 1.0 2013/07/16 11:49:42 9 9 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:34:33 9 9 CHANGE_FLAGS 2.0 2014/11/20 09:56:54 9 9 Silicate_Correction 1.0 2018/02/20 14:42:20 9 9 SORT 3.6 2020/12/17 16:02:20 9 9 REORDER 1.3.1 2021/11/24 14:31:19 ? ? MERGE 3.5.1 2021/11/24 14:31:28 9 9 SORT 3.6 2021/11/24 14:31:35 9 9 CLEAN 5.2.4 2021/11/24 14:31:44 9 9 HDREDIT2 3.2 2021/11/24 14:32:10 ? ? HDREDIT2 3.2 2021/11/24 14:32:29 ? ? Silicate_Modification 1.2 2023/03/19 10:46:20 9 9 $END $REMARKS -CLEAN functions: 2010/05/21 14:19:55 Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data_Processing\2009-64\processing\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2010/06/23 10:40:42 Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved, Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate Primary file : Q:\Cruise_Data_Processing\2009-64\Processing\hydro\\2009-64-0001.samavg Secondary file: Q:\Cruise_Data_Processing\2009-64\Processing\hydro\\2009-64-0001.mrgcln1 -CLEAN functions: 2010/06/23 10:40:49 Remove Sea-Bird comments from the header. -CALIB parameters: 2010/06/23 10:46:22 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data_Processing\2009-64\Processing\doc\2009-64-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 9 Fluorescence:Seapoint mg/m^3 10 0.0000000E+00 0.1000000E+00 18 Oxygen:Dissolved:SBE mL/L 10 0.6960000E-01 0.1020300E+01 -SORT parameters: 2010/06/23 11:16:44 Sorted in ascending order of channel Pressure -REMOVECH 2010/06/23 11:18:59 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved [ ]' changed from mL/L to umol/kg -HEADER EDITS: 2010/06/23 11:44:39 Applied edit header: Q:\Cruise_Data_Processing\2009-64\Processing\doc\2009-64-bot-hdr.txt Channel 2: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 5: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 6: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 7: PAR:Reference [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 10: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 15: Flag:Salinity:Bottle [n/a] Units: ==> n/a Channel 16: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Format: F8.3 ==> F7.2 Channel 17: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 18: Oxygen:Dissolved [mL/L] Units: ==> mL/L Channel 19: Flag:Oxygen:Dissolved [n/a] Units: ==> n/a -CALIB parameters: 2010/10/18 11:13:02 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data_Processing\Trans Cor\2009-64\DOC\2010-64-recal-tr.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 4 Transmissivity %/metre 10 0.0000000E+00 0.2087000E+01 -HEADER EDITS: 2010/10/18 11:14:38 Applied edit header: Q:\Cruise_Data_Processing\Trans Cor\2009-64\DOC\Trans_Correction-2009-64.TXT Channel 4: Transmissivity [%/metre] Format: F7.2 ==> F6.1 -SORT parameters: 2020/12/17 16:02:20 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/24 14:31:28 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2009\2009-064_Nov2021\Processing\ IOS\2009-064-0001.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2009\2009-064_Nov2021\Processing\ IOS\2009-064-0001.hplc -SORT parameters: 2021/11/24 14:31:35 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/24 14:31:44 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/24 14:32:10 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2009\2009-064\Processing\Doc\ 2009-064-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 28: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 Channel 21: Silicate:Corrected [umol/L] Format: F6.1 ==> F7.2 -HEADER EDITS: 2021/11/24 14:32:29 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2009\2009-064\Processing\Doc\ 2009-064-bot-hdr2.txt $END *COMMENTS ===================================================================================== Silicate correction was applied after the original processing. 2023/03/19 ------------------------------------------------------------------------------------- Silicate data has been adjusted to account for salinity effect and analysis on the Water Properties Group Astoria Pacific continuous flow nutrient analyser. Adjustment was made for salinity values (when available) of <= 25 psu. Adjustment equation: Silicate:Corrected = Silicate * [1+((-0.003)*(32-S))] where, Silicate:Corrected = corrected silicate concentration in micromoles/litre Silicate = uncorrected silicate concentration in micromoles/litre -0.003 = salt correction factor 32 = salt concentration of artificial seawater used by the WPG Astoria Pacific continuous flow analyser S = salinity of the sample At Station: S1, Sample Number: 9, Silicate corrected using CTD salinity. ===================================================================================== Comments from secondary file: Q:\Cruise_Data_Processing\2009-64\Processing\hydro\\2009-64-0001.mrgcln1 ------------------------------------------------------------------------ Comments from secondary file: Q:\Cruise_Data_Processing\2009-64\Processing\hydro\2009-64-0001.chl ------------------------------------------------------------------- Sample_Number 6: CHL: duplicates varied by > 10% Sample_Number 9: CHL: duplicates varied by > 10% Comments from secondary file: Q:\Cruise_Data_Processing\2009-64\Processing\hydro\2009-64-0001.add ------------------------------------------------------------------- Sample_Number 1: DO: outlier in COMPARE. Sample_Number 3: DO: bubble on probe, overshot endpoint Sample_Number 4: DO: recalc. after dosimat reset error; but looks ok in COMPARE. Analysis methods: ----------------- Chlorophyll samples are collected in duplicate in calibrated polycarbonate bottles (~330ml), then filtered onto 25mm MFS GF/75 filters at <70mmHg vacuum. The filters are placed in glass scintillation vials and immediately frozen at -20ēC until analysis. The chlorophyll is extracted in 10 ml of 90% acetone for 24h at -20ēC, then brought to room temperature and the extract is decanted and measured using a Turner Designs 10-AU-005-CE fluorometer, which has been calibrated with solutions made from commercially prepared pure chlorophyll a. Fluorometer readings are taken before and after acidification of the extract with two drops of 1.5N HCl. Average of two samples is reported. Variability is assessed as the % (std dev/mean*100) and samples are flagged if >10%. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a and Chlide-a. For further information see file QF 2009-064_HPLC*.xlsx. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965). Samples were analyzed at sea using an automated titration system consisting of a Brinkman Dosimat (model 665) and a PC 950 Colorimeter. Endpoint titers were optimized in post-processing using a best fit linear regression utility. File 2009-64oxygen.xls includes the raw data and duplicate analysis. Salinity samples were collected in glass bottles and analyzed on Guildline Autosal model 8400B, S/N 69086, at IOS. Salinometers are standardized with IAPSO standard seawater. Nutrient samples were collected in plastic tubes and stored frozen and later analysed at IOS using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). File "QF 2009-64nuts.xls" includes the raw data and an analysis of duplicates. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. J. Barwell-Clarke and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 3. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. ******************************************************************************** Transmissometer #1005DR was calibrated in March 2008, and drifted significantly but steadily until July 2009. Then a sudden shift occurred so that values between September 2009 and July 2010 were very low, ~25%/m. After the shift, drift continued at a similar rate to that before the shift. There is some pressure dependence after the shift, so that transmissivity is decreasing with pressure even in deep offshore waters. In August 2010 a study was made of transmissivity that led to a decision to apply post-processing corrections to all cruises between March 2008 and June 2010. Transmissivity data from this cruise were corrected by multiplying the original values by a correction factor 2.087, which was based on assumptions that deep offshore transmissivity from February 2010 and June 2010 Line P cruises should be about 62%/m and that drift was linear with time. The corrections produced reasonable results for other cruises in late 2009. For details on how the correction factor was derived see: OSD_Data_Archive:\Cruise_Data\DOCUMENTS\Transmissometer 1005DR Corrections.doc These data should be considered estimates and even the shape may not be as reliable as usual. ******************************************************************************** *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ----------------------- --------- ---- ------ ------------ Fluorescence:Seapoint mg/m^3 10 -99 () (0 0.1) Oxygen:Dissolved:SBE mL/L 10 -99 () (0.696E-01 1.0203) Transmissivity %/metre 10 -99 () (0 2.087) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- --8--- ---9---- --10-- --11- -12- ---13--- 14 ---15-- 16 --17- 18 --19-- 20 --21-- 22 --23-- 24 25 ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- 48 !Samp Bott Pressu Temperat Trans Fluores PAR PAR: Salinity Oxygen Oxyge Numb Salinity Fl Oxygen: Fl Nitra Fl Silica Si Phosph Fl Chloro Fl Bo HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ re ure: missi cence: Refere :T0:C0 : n: er_o :Bottle ag Dissolv ag te_ ag te li ate ag phyll: ag tt Chl-c3 Chlide- Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb Primary vity URU: nce Dissol Disso ~bin ~o ed ~o plus_ ~t ~: ~p Extrac ~a ~i a a fuco fuco : !er er Seapoin ved: lved: _rec tt lv Nitri ri Fl ha ted ct ti HP ! t SBE SBE ords le ed te te ag te ed on LC !---- ---- ------ -------- ----- ------- ------ ------ -------- ------ ----- ---- -------- -- ------- -- ----- -- ------ -- ------ -- ------ -- -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 9 9 1.6 9.1507 1.9 1.736 246.9 316.9 23.2951 6.13 261.5 121 -99.0000 0 5.963 0 24.4 0 59.30 0 2.26 0 0.65 63 9 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 8 8 5.6 9.1663 30.5 1.256 19.0 315.5 27.6987 5.53 234.8 121 -99.0000 0 5.373 0 26.7 0 57.90 0 2.39 0 -99.00 0 8 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 7 7 10.5 9.2011 35.5 0.810 4.4 313.2 28.2499 5.31 226.8 121 -99.0000 0 5.190 0 27.1 0 56.80 0 2.40 0 0.21 6 7 0.004 0.000 0.015 0.000 0.000 0.006 0.099 0.007 0.014 0.007 0.000 0.005 0.009 0.000 0.002 0.001 0.066 0.000 0.293 0.000 0.005 0.293 6 6 6 21.0 9.3224 47.8 0.249 1.0 307.7 29.7716 4.61 197.3 121 -99.0000 0 4.522 0 27.9 0 54.80 0 2.43 0 0.08 63 6 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 5 5 30.7 9.7864 48.8 0.166 0.6 315.5 30.1097 3.77 162.6 121 -99.0000 0 3.726 0 27.7 0 55.10 0 2.45 0 -99.00 0 5 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 4 4 41.1 10.0103 51.3 0.145 0.6 361.2 30.3013 3.45 145.5 121 -99.0000 0 3.335 0 27.4 0 53.80 0 2.51 0 -99.00 0 4 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 3 3 51.0 10.0998 53.0 0.113 0.6 403.1 30.4266 3.22 -99.0 121 -99.0000 0 -99.000 5 27.3 6 52.70 6 2.48 6 -99.00 0 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 2 2 76.0 9.5319 47.0 0.103 0.5 439.0 30.7260 3.73 154.8 121 -99.0000 0 3.550 0 27.8 0 54.10 0 2.55 0 -99.00 0 2 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1 1 100.7 9.4185 45.5 0.082 0.5 472.8 31.1232 0.37 29.7 121 31.1147 0 0.682 3 26.5 0 72.60 0 3.56 0 -99.00 0 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0