*2021/11/23 15:47:06.03 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2006/04/18 17:42:24.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 8 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 3C314310 NUMBER OF CHANNELS : 37 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 192 199 2 Bottle_Number n/a 1 8 3 Pressure decibar 1.8 29.9 4 Temperature:Primary 'deg C (ITS90)' 8.4417 8.9152 5 Transmissivity %/metre 18.7 54.7 6 Fluorescence:URU:Seapoint mg/m^3 0.39 1.438 7 PAR uE/m^2/sec 1.5 583 8 PAR:Reference uE/m^2/sec 2400.1 2546.4 9 Salinity:T0:C0 PSS-78 26.4212 29.6037 10 Oxygen:Dissolved:SBE mL/L 4.88 6.64 11 Oxygen:Dissolved:SBE umol/kg 213.1 290.7 12 Number_of_bin_records n/a 121 121 13 Chlorophyll:Extracted mg/m^3 0.77 1.51 14 Flag:Chlorophyll:Extracted n/a 15 HPLC:Chl-c3 mg/m^3 0 0.14E-01 16 HPLC:Chlide-a mg/m^3 0 0.17E-01 17 HPLC:Chl-c2 mg/m^3 0.74E-01 0.163 18 HPLC:Peri mg/m^3 0.18E-01 0.33E-01 19 HPLC:Pheide-a mg/m^3 0 0 20 HPLC:But-fuco mg/m^3 0 0 21 HPLC:Fuco mg/m^3 0.309 0.791 22 HPLC:Neo mg/m^3 0 0 23 HPLC:Pras mg/m^3 0 0 24 HPLC:Viola mg/m^3 0 0 25 HPLC:Hex-fuco mg/m^3 0 0 26 HPLC:Diadino mg/m^3 0.48E-01 0.52E-01 27 HPLC:Allo mg/m^3 0 0.31E-01 28 HPLC:Diato mg/m^3 0 0 29 HPLC:Zea mg/m^3 0 0 30 HPLC:Lut mg/m^3 0 0 31 HPLC:Chl-b mg/m^3 0 0 32 HPLC:DVChl-a mg/m^3 -99 -99 33 HPLC:Chl-a mg/m^3 0.767 1.486 34 HPLC:Phe mg/m^3 0 0 35 HPLC:B-Car mg/m^3 0.16E-01 0.27E-01 36 HPLC:TChl-a mg/m^3 0.767 1.503 37 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 6 F ' ' 1 12 -99 ' ' 5 I I 0 13 -99 ' ' 7 F R4 2 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2006-11 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : SoG/JdeF SCIENTIST : Pena A. PLATFORM : Vector *LOCATION GEOGRAPHIC AREA : Strait of Georgia/Juan de Fuca Strait STATION : 42 EVENT NUMBER : 43 LATITUDE : 49 1.87000 N ! (deg min) LONGITUDE : 123 26.27000 W ! (deg min) *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 $TABLE: SENSORS ! Name Abs Depth Serial No ! ------------------------- -------------- ---------- temperature:primary ' ' 2968 conductivity:primary ' ' 1766 pressure ' ' 63507 temperature:secondary ' ' 2106 conductivity:secondary ' ' 1729 Fluorometer ' ' Seapoint transmissometer:primary ' ' 723DR Oxygen:SBE ' ' 0766 altimeter: ' ' ' ' irradiance:primary ' ' 4565 surface ' ' 'degrees = 0.0' $END $REMARKS SOFTWARE VERSION SEASAVE WIN32 V 5.29B $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 3.0 2006/06/12 13:40:50 968 968 ADDSAMP 3.4 2006/06/14 17:21:31 968 968 BINAVE 4.1 2006/06/14 17:32:48 968 8 MERGE 3.3 2006/07/12 15:23:21 8 8 CLEAN 5.0 2006/07/12 15:23:27 8 8 CALIB 11.7 2006/07/12 15:24:37 8 8 SORT 3.5 2006/07/12 16:22:01 8 8 REMOVECH 7.0 2006/07/12 16:22:11 8 8 CHGUNITS 3.0 2006/07/12 16:22:18 8 8 REORDER 1.2.1 2006/07/12 16:22:24 ? ? HDREDIT2 2.5.1 2006/07/12 16:25:14 ? ? HDREDIT2 2.5.1 2006/12/12 18:03:16 ? ? CHANGE_FLAGS 1.0 2013/07/16 11:48:20 8 8 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:32:30 8 8 CHANGE_FLAGS 2.0 2014/11/20 09:55:06 8 8 SORT 3.6 2021/01/13 09:21:11 8 8 REORDER 1.3.1 2021/11/23 15:45:49 ? ? MERGE 3.5.1 2021/11/23 15:46:08 8 8 SORT 3.6 2021/11/23 15:46:15 8 8 CLEAN 5.2.4 2021/11/23 15:46:21 8 8 HDREDIT2 3.2 2021/11/23 15:46:46 ? ? HDREDIT2 3.2 2021/11/23 15:47:06 ? ? $END $REMARKS -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data\2006-11\CTD\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2006/07/12 15:23:19 2006/07/12 15:23:19 2006/07/12 15:23: Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle, Chlorophyll:Extracted, Flag:Chlorophyll:Extracted, Oxygen:Dissolved, Flag:Oxygen:Dissolved Primary file : Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.samavg Secondary file: Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.mrg4 -MERGE *** Secondary file not found *** Primary file : Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.cst Secondary file: Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.sal -The following MERGE parameters were used: 2006/06/14 17:38:38 2006/06/14 17:38:38 2006/06/14 17:38: Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Chlorophyll:Extracted, Flag:Chlorophyll:Extracted Primary file : Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.mrg1 Secondary file: Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.chl -MERGE *** Secondary file not found *** Primary file : Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.mrg2 Secondary file: Q:\Cruise_Data\2006-11\CTD\hydro\2006-11-0043.add -MERGE *** Secondary file not found *** Primary file : Q:\Cruise_Data\2006-11\ctd\hydro\2006-11-0043.mrg3 Secondary file: Q:\Cruise_Data\2006-11\ctd\hydro\2006-11-0043.nuts -CLEAN functions: 2006/07/12 15:23:26 20 Reset #RECS, MIN & MAX values in header. Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CALIB parameters: 2006/07/12 15:24:37 Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data\2006-11\CTD\doc\2006-11-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 11 Oxygen:Dissolved:SBE mL/L 10 0.7830000E-01 0.1066300E+01 -SORT parameters: 2006/07/12 16:22:01 Sorted in ascending order of channel Pressure -REMOVECH 2006/07/12 16:22:11 The following CHANNEL(S) were removed: Scan_Number Temperature:Secondary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Descent_Rate [m/s] Altimeter [metres] Status:Pump Salinity:T1:C1 [PSS-78] Flag -CHANGE units: 'Oxygen:Dissolved:SBE' changed from mL/L to umol/kg -HEADER EDITS: 2006/07/12 16:25:14 Applied edit header: Q:\Cruise_Data\2006-11\CTD\doc\2006-11-BOT-HDR.TXT Channel 2: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 5: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 6: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 8: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 9: PAR:Reference [uE/m^2/sec] Format: F11.3 ==> F7.1 -HEADER EDITS: 2006/12/12 18:03:16 Applied edit header: Q:\Cruise_Data\Bottle Header Fix\fix-hdr-qual.txt -SORT parameters: 2021/01/13 09:21:11 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/23 15:46:08 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2006\2006-011_Nov2021\Processing\ IOS\2006-011-0043.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2006\2006-011_Nov2021\Processing\ IOS\2006-011-0043.hplc -SORT parameters: 2021/11/23 15:46:15 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/23 15:46:21 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/23 15:46:46 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2006\2006-011\Processing\Doc\ 2006-011-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 15: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/23 15:47:06 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2006\2006-011\Processing\Doc\ 2006-011-bot-hdr2.txt $END *COMMENTS Sample_Number 192: Extracted Chlorophyll: variability>10% Analysis methods: ----------------- Salinity samples are collected in glass bottles and analyzed on a Guildline Autosal 8400B Salinometer which was standardized with IAPSO standard seawater. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965). A Brinkmann model 665 Dosimat and model PC910 Colorimeter is controlled by Visual-Basic program to titrate the oxygen samples. Nutrient samples are analyzed frozen using a Technicon AAII autoanalyzer following methods described in Barwell-Clarke and Whitney (1996). Chlorophyll samples are collected in duplicate in calibrated polycarbonate bottles (~330ml), then filtered onto 25mm MFS GF/75 filters at <70mmHg vacuum. The filters are placed in glass scintillation vials and immediately frozen at -20ēC until analysis. The chlorophyll is extracted in 10 ml of 90% acetone for 24h at -20ēC, then brought to room temperature and the extract is decanted and measured using a Turner Designs 10-AU-005-CE fluorometer, which has been calibrated with solutions of commercially prepared chlorophyll. Average of two samples is reported unless stated otherwise. Variability is assessed as the % (std dev/mean*100). HPLC samples were filtered onto 25mm GF/F filters and stored at -80C prior to analysis. Replicates were extracted in 95% methanol by different methods, either -20C soakfor 24 hours or 1-4 hr soak after sonication and analyzed n a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~14 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a and Chlide-a. For further information see file QF 2006-011_HPLC*.xlsx. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. J. Barwell-Clarke and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 3. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. ------------------------------------------------------------------------------ Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2006\2006-011_Nov2021\Processing\IOS\2006-011-0043. hplc --------------------------------------------------------------------------- Sample_Number 195: HPLC: >10%CV for TChl-a Sample_Number 198: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.783E-01 1.0663) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- --8--- ---9---- --10-- --11- -12- --13-- 14 ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- 37 !Samp Bott Pressu Temperat Trans Fluores PAR PAR: Salinity Oxygen Oxyge Numb Chloro Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ re ure: missi cence: Refere :T0:C0 : n: er_o phyll: ag Chl-c3 Chlide- Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb Primary vity URU: nce Dissol Disso ~bin Extrac ~a a a fuco fuco : !er er Seapoin ved: lved: _rec ted ct HP ! t SBE SBE ords ed LC !---- ---- ------ -------- ----- ------- ------ ------ -------- ------ ----- ---- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 198 7 1.8 8.8925 19.1 0.692 583.0 2541.1 26.4212 6.64 290.7 121 0.90 0 0.000 0.000 0.074 0.018 0.000 0.000 0.309 0.000 0.000 0.000 0.000 0.052 0.031 0.000 0.000 0.000 0.000 -99.000 0.767 0.000 0.016 0.767 36 199 8 1.8 8.8906 18.7 0.591 570.9 2529.8 26.4238 6.64 290.4 121 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 197 6 5.3 8.9152 26.2 0.831 100.7 2514.4 27.5435 6.49 283.8 121 1.23 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 195 4 10.4 8.4878 48.9 1.127 23.5 2546.4 29.0641 6.16 269.1 121 1.51 0 0.014 0.017 0.163 0.033 0.000 0.000 0.791 0.000 0.000 0.000 0.000 0.048 0.000 0.000 0.000 0.000 0.000 -99.000 1.486 0.000 0.027 1.503 36 196 5 10.4 8.4897 48.9 1.235 23.3 2541.5 29.0601 6.17 269.3 121 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 194 3 20.0 8.4446 51.5 1.244 4.9 2400.1 29.2730 5.96 260.1 121 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 193 2 20.1 8.4417 51.5 1.438 5.0 2432.9 29.2743 5.96 260.1 121 1.15 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 192 1 29.9 8.5002 54.7 0.390 1.5 2519.0 29.6037 4.88 213.1 121 0.77 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0