*2021/11/23 14:38:03.84 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2005/03/04 16:10:28.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 17 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII NUMBER OF CHANNELS : 50 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 183 199 2 Bottle_Number n/a 1 17 3 Pressure decibar 2.4 261.7 4 Temperature:Secondary 'deg C (ITS90)' 7.7692 9.475 5 Transmissivity %/metre 12.5 45.8 6 Fluorescence:URU:Seapoint mg/m^3 0.159 7.951 7 PAR uE/m^2/sec 0.5 130.7 8 PAR:Reference uE/m^2/sec 409.1 485.9 9 Salinity:T1:C1 PSS-78 25.8481 30.929 10 Oxygen:Dissolved:SBE mL/L 3.06 7.13 11 Number_of_bin_records n/a 61 121 12 Salinity:Bottle PSS-78 30.9222 30.9222 13 Flag:Salinity:Bottle n/a 14 Oxygen:Dissolved mL/L -99.99 7.161 15 Flag:Oxygen:Dissolved n/a 16 Nitrate_plus_Nitrite umol/L 19.3 29.5 17 Flag:Nitrate_plus_Nitrite n/a 18 Silicate umol/L 39 62.3 19 Flag:Silicate n/a 20 Phosphate umol/L 1.6 2.8 21 Flag:Phosphate n/a 22 Chlorophyll:Extracted mg/m^3 0.38 17.72 23 Flag:Chlorophyll:Extracted n/a 24 HPLC:Chl-c3 mg/m^3 0 0.36E-01 25 HPLC:Chlide-a mg/m^3 0 0.155 26 HPLC:MgDVP mg/m^3 0 0.103 27 HPLC:Chl-c2 mg/m^3 0.67E-01 0.669 28 HPLC:Chl-c1 mg/m^3 0.51E-01 0.617 29 HPLC:Me-chlide mg/m^3 0 0.127 30 HPLC:Peri mg/m^3 0 0.6E-02 31 HPLC:Pheide-a mg/m^3 0 0 32 HPLC:But-fuco mg/m^3 0 0.21E-01 33 HPLC:Fuco mg/m^3 0.687 6.549 34 HPLC:Neo mg/m^3 0 0.38E-01 35 HPLC:Pras mg/m^3 0 0.46E-01 36 HPLC:Viola mg/m^3 0 0 37 HPLC:Hex-fuco mg/m^3 0 0 38 HPLC:Diadino mg/m^3 0.49E-01 0.481 39 HPLC:Allo mg/m^3 0 0.21E-01 40 HPLC:Diato mg/m^3 0 0 41 HPLC:Zea mg/m^3 0.2E-01 0.48E-01 42 HPLC:Lut mg/m^3 0 0.3E-02 43 HPLC:Chl-b mg/m^3 0 0.16E-01 44 HPLC:C2mgdg mg/m^3 0 0.5E-02 45 HPLC:DVChl-a mg/m^3 0 0 46 HPLC:Chl-a mg/m^3 1.145 9.572 47 HPLC:Phe mg/m^3 0 0 48 HPLC:B-Car mg/m^3 0.28E-01 0.199 49 HPLC:TChl-a mg/m^3 1.145 9.854 50 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 5 I I 0 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 8 F ' ' 3 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 6 F R4 1 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 6 F R4 1 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 3 NQ C ' ' 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2005-02 AGENCY : IOS, Ocean Science and Productivity, Sidney, B.C. COUNTRY : Canada PROJECT : Optical Properties Off Vancouver Island SCIENTIST : Pena A. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI/Strait of Georgia STATION : BIO2 EVENT NUMBER : 31 LATITUDE : 49 0.46000 N ! (deg min) LONGITUDE : 123 23.11000 W ! (deg min) ALTIMETER (M) : 11.97 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 $TABLE: SENSORS ! Name Abs Depth Serial No ! ------------------------- -------------- ---------- temperature:primary ' ' 2449 conductivity:primary ' ' 2102 pressure ' ' 63507 temperature:secondary ' ' 2023 conductivity:secondary ' ' 1766 Fluorometer ' ' Seapoint transmissometer:primary ' ' 333DR Oxygen:SBE ' ' 0766 irradiance:primary ' ' 4656 altimeter: ' ' ' ' surface ' ' 'degrees = 0.0' $END $REMARKS SOFTWARE VERSION SEASAVE WIN32 V 5.32 Comments from secondary file: Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.add ------------------------------------------------- Dissolved oxygen concentration determined using an automated titration system consisting of a Brinkman dosimat (model 665) and a PC 900 Colorimeter. $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 2.9 2005/04/04 15:22:53 1942 1942 CLEAN 4.7.1 2005/04/04 15:25:02 1942 1942 ADDSAMP 3.4 2005/04/19 09:39:42 1942 1942 BINAVE 4.1 2005/04/19 09:39:59 1942 17 MERGE 3.3 2005/04/19 10:14:06 17 17 MERGE 3.3 2005/04/19 10:16:46 17 17 CALIB 11.4 2005/04/19 15:27:17 17 17 CLEAN 4.7.1 2005/04/20 14:54:37 ? ? SORT 3.5 2005/04/20 14:54:51 17 17 REMOVECH 7.0 2005/04/20 15:29:08 17 17 HDREDIT2 2.4 2005/04/20 16:09:56 ? ? SORT 3.5 2005/05/29 12:17:09 17 17 MERGE 3.3 2005/05/29 12:17:14 17 17 SORT 3.5 2005/05/29 14:58:19 17 17 SORT 3.5 2005/07/05 16:56:15 17 17 MERGE 3.3 2005/07/05 16:56:45 17 17 SORT 3.5 2005/07/05 17:00:34 17 17 CHANGE_FLAGS 1.0 2013/07/16 11:47:58 17 17 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:31:56 17 17 CHANGE_FLAGS 2.0 2014/11/20 09:54:40 17 17 CLEAN 5.2.3 2018/07/06 08:45:41 ? ? SORT 3.6 2021/03/22 08:36:50 17 17 REORDER 1.3.1 2021/11/23 14:37:00 ? ? MERGE 3.5.1 2021/11/23 14:37:09 17 17 SORT 3.6 2021/11/23 14:37:15 17 17 CLEAN 5.2.4 2021/11/23 14:37:22 17 17 HDREDIT2 3.2 2021/11/23 14:37:47 ? ? HDREDIT2 3.2 2021/11/23 14:38:03 ? ? $END $REMARKS -CLEAN functions: 2005/04/04 15:24:37 20 Interpolation by Channel 1: Scan_Number [n/a] in Pressure Change Pad Value to -99 in Channel: Oxygen:Dissolved:SBE [mL/L] Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data\2005-02\ctd\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 0.50 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following MERGE parameters were used: 2005/04/19 10:14:05 2005/04/19 10:14: Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Chlorophyll:Extracted, Flag:Chlorophyll:Extracted Primary file : Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.samavg Secondary file: Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.chl -The following MERGE parameters were used: 2005/04/19 10:16:46 2005/04/19 10:16: Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Oxygen:Dissolved, Flag:Oxygen:Dissolved Primary file : Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.mrg1 Secondary file: Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.add Comments from secondary file: Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.add ------------------------------------------------- C.H. Culbertson's paper on Dissolved Oxygen in the 'Woce Hydrographic Program Operations and Methods - July 1991' -The following CALIB parameters were used: Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data\2005-02\ctd\doc\2005-02-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 11 Oxygen:Dissolved:SBE mL/L 10 0.1110000E+00 0.1463000E+01 -CLEAN functions: 2005/04/20 14:54:36 20 Remove Sea-Bird comments from the header. -SORT parameters: 2005/04/20 14:54:51 Sorted in ascending order of channel Pressure -REMOVECH 2005/04/20 15:29:08 The following CHANNEL(S) were removed: Scan_Number Temperature:Primary [deg C (ITS90)] Conductivity:Primary [S/m] Conductivity:Secondary [S/m] Oxygen:Voltage:SBE [volts] Altimeter [metres] Descent_Rate [m/s] Salinity:T0:C0 [PSS-78] Flag -HEADER EDITS: 2005/04/20 16:09:56 Applied edit header: Q:\Cruise_Data\2005-02\ctd\doc\2005-02-bot-hdr.txt Channel 2: Pressure [decibar] Units: dbars ==> decibar Format: F10.3 ==> F7.1 Channel 5: Fluorescence:URU:Seapoint [mg/m^3] Name: Fluorescence:Seapoint ==> Fluorescence:URU:Seapoint Channel 6: Oxygen:Dissolved:SBE [mL/L] Format: F8.3 ==> F7.2 Channel 7: PAR [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 8: PAR:Reference [uE/m^2/sec] Format: F11.3 ==> F7.1 Channel 12: Chlorophyll:Extracted [mg/m^3] Units: ==> mg/m^3 Channel 13: Flag:Chlorophyll:Extracted [n/a] Units: ==> n/a Channel 15: Flag:Oxygen:Dissolved [n/a] Units: newunits ==> n/a -SORT parameters: 2005/05/29 12:17:09 Sorted in ascending order of channel Sample_Number -The following MERGE parameters were used: 2005/05/29 12:17:13 2005/05/29 12:17:13 2005/05/29 12:17: Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Salinity:Bottle, Flag:Salinity:Bottle Primary file : Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.pche2 Secondary file: Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.sal -SORT parameters: 2005/05/29 14:58:19 Sorted in ascending order of channel Pressure -SORT parameters: 2005/07/05 16:56:15 Sorted in descending order of channel Sample_Number -The following MERGE parameters were used: 2005/07/05 16:56:44 2005/07/05 16:56:44 2005/07/05 16:56: Merge Channel: Sample_Number Merge Scheme Used: Interweave Records from Both Files Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Nitrate_plus_Nitrite, Flag:Nitrate_plus_Nitrite, Silicate, Flag:Silicate, Phosphate, Flag:Phosphate Primary file : H:\Data Processing\2005\2005-02\NUts\2005-02-0031.srt_che Secondary file: H:\Data Processing\2005\2005-02\NUts\2005-02-0031.ios -SORT parameters: 2005/07/05 17:00:34 Sorted in ascending order of channel Pressure -CLEAN functions: 2018/07/06 08:45:41 20 Change Pad Value to -99 in All Channels. -SORT parameters: 2021/03/22 08:36:50 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/23 14:37:09 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2005\2005-002_Nov2021\Processing\ IOS\2005-002-0031.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2005\2005-002_Nov2021\Processing\ IOS\2005-002-0031.hplc -SORT parameters: 2021/11/23 14:37:15 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/23 14:37:22 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/23 14:37:47 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2005\2005-002\Processing\Doc\ 2005-002-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 24: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Chl-c1 [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Me-chlide [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 36: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 37: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 38: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 39: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 40: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 41: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 42: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 43: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 44: HPLC:C2mgdg [mg/m^3] Units: ==> mg/m^3 Channel 45: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 46: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 47: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 48: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 49: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/23 14:38:03 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2005\2005-002\Processing\Doc\ 2005-002-bot-hdr2.txt $END *COMMENTS Comments from secondary file: Q:\Cruise_Data\2005-02\ctd\hydro\2005-02-0031.chl ------------------------------------------------- Sample_Number 191: variability > 10% Sample_Number 192: variability > 10% Analysis methods: ----------------- Salinity samples are collected in glass bottles and analyzed on Guildline model 8410 Portasal Salinometers which are standardized with IAPSO standard seawater. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965). A Brinkmann model 665 Dosimat and model PC910 Colorimeter is controlled by Visual-Basic program to titrate the oxygen samples. Nutrient samples are analyzed frozen using a Technicon AAII autoanalyzer following methods described in Barwell-Clarke and Whitney (1996). Chlorophyll samples are collected in duplicate in calibrated polycarbonate bottles (~330ml), then filtered onto 25mm MFS GF/75 filters at <70mmHg vacuum. The filters are placed in glass scintillation vials and immediately frozen at -20ēC until analysis. The chlorophyll is extracted in 10 ml of 90% acetone for 24h at -20ēC, then brought to room temperature and the extract is decanted and measured using a Turner Designs 10-AU-005-CE fluorometer, which has been calibrated with solutions of commercially prepared chlorophyll. HPLC samples were filtered onto 25mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~3 years after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a and DVchl-a, and Chlide-a and Me-chlide. For further information see file QF 2005-002_HPLC*.xlsx. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. J. Barwell-Clarke and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 3. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2005\2005-002_Nov2021\Processing\IOS\2005-002-0031. hplc --------------------------------------------------------------------------- Sample_Number 194: HPLC: TChl-a low compared to Turner values, all pigments may be underestimated Sample_Number 198: HPLC: TChl-a low compared to Turner values, all pigments may be underestimated *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.111 1.463) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- --8--- ---9---- --10-- -11- ---12--- 13 ---14-- 15 --16- 17 --18- 19 --20-- 21 --22-- 23 ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- 50 !Samp Bott Pressu Temperat Trans Fluores PAR PAR: Salinity Oxygen Numb Salinity Fl Oxygen: Fl Nitra Fl Silic Fl Phosph Fl Chloro Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ re ure: missi cence: Refere :T1:C1 : er_o :Bottle ag Dissolv ag te_ ag ate ag ate ag phyll: ag Chl-c3 Chlide- MgDVP Chl-c2 Chl-c1 Me- Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b C2mgdg DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb Secondar vity URU: nce Dissol ~bin ~o ed ~o plus_ ~t ~i ~p Extrac ~a a chlide a fuco fuco : !er er y Seapoin ved: _rec tt lv Nitri ri ca ha ted ct HP ! t SBE ords le ed te te te te ed LC !---- ---- ------ -------- ----- ------- ------ ------ -------- ------ ---- -------- -- ------- -- ----- -- ----- -- ------ -- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 199 17 2.4 7.7692 12.5 7.951 130.7 479.6 25.8481 7.13 121 -99.0000 0 7.161 0 19.3 0 39.0 0 1.60 0 17.72 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 197 15 4.8 7.8397 21.2 6.644 26.7 481.7 27.7276 6.51 61 -99.0000 0 6.571 0 22.2 0 43.1 0 1.86 0 15.85 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 198 16 4.8 7.8401 20.5 7.192 26.6 481.7 27.7411 6.51 120 -99.0000 0 -99.990 0 -99.0 0 -99.0 0 -99.00 0 -99.00 0 0.036 0.155 0.103 0.669 0.617 0.127 0.006 0.000 0.021 6.549 0.038 0.046 0.000 0.000 0.481 0.021 0.000 0.048 0.000 0.016 0.005 0.000 9.572 0.000 0.199 9.854 46 196 14 9.8 8.0344 39.8 1.113 3.4 485.5 28.4627 5.50 121 -99.0000 0 5.600 0 27.8 0 54.8 0 2.28 0 2.11 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 195 13 14.8 8.1102 39.3 1.418 1.4 485.9 28.6853 5.46 121 -99.0000 0 5.767 0 27.4 0 53.2 0 2.31 0 2.59 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 193 11 19.0 8.1334 39.0 1.371 0.9 485.9 28.7426 5.40 68 -99.0000 0 5.329 0 27.9 0 56.4 0 2.35 0 2.05 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 194 12 19.0 8.1325 39.2 1.395 0.9 485.9 28.7416 5.40 120 -99.0000 0 -99.990 0 -99.0 0 -99.0 0 -99.00 0 -99.00 0 0.000 0.000 0.000 0.067 0.051 0.000 0.000 0.000 0.000 0.687 0.000 0.000 0.000 0.000 0.049 0.000 0.000 0.020 0.003 0.000 0.000 0.000 1.145 0.000 0.028 1.145 46 192 10 23.8 8.4661 44.0 0.472 0.7 481.7 29.2834 4.88 121 -99.0000 0 4.887 0 29.2 0 56.1 0 2.50 0 0.60 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 191 9 28.8 8.4826 44.9 0.394 0.6 475.4 29.3560 4.83 121 -99.0000 0 4.881 0 29.5 0 56.5 0 2.44 0 0.38 3 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 190 8 48.6 8.1921 37.9 0.377 0.5 471.3 29.6953 4.93 121 -99.0000 0 5.153 0 28.9 0 53.8 0 2.37 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 189 7 74.1 8.1031 39.6 0.241 0.5 471.3 29.8566 4.97 121 -99.0000 0 4.919 0 28.8 0 54.1 0 2.36 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 188 6 99.3 8.1596 39.4 0.240 0.5 460.4 30.0183 4.87 121 -99.0000 0 4.878 0 25.0 0 47.2 0 2.34 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 187 5 124.0 8.4689 42.5 0.191 0.5 444.6 30.1982 4.50 121 -99.0000 0 5.025 0 29.0 0 54.1 0 2.42 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 186 4 149.2 8.4783 39.3 0.193 0.5 434.0 30.3763 4.42 121 -99.0000 0 4.558 0 28.9 0 54.7 0 2.44 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 185 3 174.6 8.8640 40.8 0.177 0.5 436.9 30.5946 3.92 121 -99.0000 0 3.876 0 29.1 0 56.6 0 2.58 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 184 2 199.7 9.2798 45.8 0.159 0.5 415.6 30.7589 3.41 121 -99.0000 0 3.475 0 29.2 0 58.5 0 2.64 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 183 1 261.7 9.4750 29.4 0.167 0.5 409.1 30.9290 3.06 121 30.9222 0 3.158 0 29.4 0 62.3 0 2.80 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0