*2021/11/23 10:41:56.62 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2004/04/29 02:49:39.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 6 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII NUMBER OF CHANNELS : 36 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 108 113 2 Bottle_Number n/a 1 6 3 Pressure decibar 1 36 4 Temperature:Primary 'deg C (ITS90)' 8.9537 11.5369 5 Transmissivity %/metre 16.6 38.8 6 Fluorescence:URU:Seapoint mg/m^3 1.941 14.762 7 PAR uE/m^2/sec 0.5 21.6 8 PAR:Reference uE/m^2/sec 184.5 241 9 Salinity:T0:C0 PSS-78 25.9709 29.6744 10 Oxygen:Dissolved:SBE mL/L 5.58 8.04 11 Number_of_bin_records n/a 116 121 12 Chlorophyll:Extracted mg/m^3 1.9 13.89 13 Flag:Chlorophyll:Extracted n/a 14 HPLC:Chl-c3 mg/m^3 0 0.253 15 HPLC:Chlide-a mg/m^3 0.17E-01 0.505 16 HPLC:Chl-c2 mg/m^3 0.21 1.594 17 HPLC:Peri mg/m^3 0.13E-01 0.56E-01 18 HPLC:Pheide-a mg/m^3 0 1.317 19 HPLC:But-fuco mg/m^3 0 0.35E-01 20 HPLC:Fuco mg/m^3 0.872 5.089 21 HPLC:Neo mg/m^3 0 0 22 HPLC:Pras mg/m^3 0 0 23 HPLC:Viola mg/m^3 0 0.15E-01 24 HPLC:Hex-fuco mg/m^3 0 0 25 HPLC:Diadino mg/m^3 0.56E-01 0.252 26 HPLC:Allo mg/m^3 0.7E-02 0.24E-01 27 HPLC:Diato mg/m^3 0 0.15E-01 28 HPLC:Zea mg/m^3 0 0.17E-01 29 HPLC:Lut mg/m^3 0 0 30 HPLC:Chl-b mg/m^3 0 0.91E-01 31 HPLC:DVChl-a mg/m^3 -99 -99 32 HPLC:Chl-a mg/m^3 2.243 11.168 33 HPLC:Phe mg/m^3 0 0.598 34 HPLC:B-Car mg/m^3 0.37E-01 0.195 35 HPLC:TChl-a mg/m^3 2.26 11.674 36 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 7 F ' ' 1 9 -99 ' ' 9 F ' ' 4 10 -99 ' ' 7 F ' ' 2 11 -99 ' ' 5 I I 0 12 -99 ' ' 7 F R4 2 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 8 F R4 3 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2004-08 AGENCY : IOS, Ocean Science and Productivity, Sidney, B.C. COUNTRY : Canada PROJECT : SoG / JdeF SCIENTIST : Pena A. PLATFORM : Vector *LOCATION GEOGRAPHIC AREA : Juan de Fuca Strait/Strait of Georgia STATION : 48 EVENT NUMBER : 29 LATITUDE : 48 51.69000 N ! (deg min) LONGITUDE : 123 3.08000 W ! (deg min) WATER DEPTH : 219 ALTIMETER (M) : 10.16 ! custom item $REMARKS Altimeter value is distance from bottom and is calculated as the median of the deepest 2 metres of data. $END *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0550 $TABLE: SENSORS ! Name Abs Depth Serial No ! ------------------------- -------------- ---------- temperature:primary ' ' 2038 conductivity:primary ' ' 2173 pressure ' ' 75636 temperature:secondary ' ' 2968 conductivity:secondary ' ' 1729 Fluorometer ' ' Seapoint Oxygen:SBE ' ' 0615 transmissometer:primary ' ' 498DR altimeter: ' ' ' ' irradiance ' ' primary surface ' ' 'degrees = 0.0' $END $REMARKS SOFTWARE VERSION SEASAVE WIN32 V 5.30A $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 2.7 2004/05/21 16:01:33 726 726 CLEAN 4.6.2 2004/05/21 16:02:45 ? ? ADDSAMP 3.4 2004/05/22 15:42:00 726 726 BINAVE 4.0.1 2004/06/07 13:22:01 726 6 MERGE 3.1 2004/06/07 16:48:44 6 6 MERGE 3.1 2004/06/07 16:49:01 6 6 CALIB 11.4 2004/06/07 16:49:45 6 6 CLEAN 4.6.2 2004/06/07 16:50:04 6 6 SORT 3.4.1 2004/06/07 16:50:08 6 6 REMOVECH 6.0 2004/06/07 16:51:38 6 6 HDREDIT2 2.1 2004/06/07 17:01:40 ? ? MERGE 3.3 2006/05/19 16:10:45 6 6 CLEAN 5.0 2006/05/24 08:19:43 6 6 CHANGE_FLAGS 1.0 2013/07/29 14:29:37 6 6 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:30:04 6 6 CHANGE_FLAGS 2.0 2014/11/20 09:54:14 6 6 CLEAN 5.2.3 2018/07/06 08:58:27 ? ? SORT 3.6 2021/01/19 08:32:12 6 6 REORDER 1.3.1 2021/11/23 10:40:28 ? ? MERGE 3.5.1 2021/11/23 10:40:54 6 6 SORT 3.6 2021/11/23 10:41:01 6 6 CLEAN 5.2.4 2021/11/23 10:41:08 6 6 HDREDIT2 3.2 2021/11/23 10:41:35 ? ? HDREDIT2 3.2 2021/11/23 10:41:56 ? ? $END $REMARKS CLEAN functions: - Set event to last 4 characters of file name -The following ADDSAMP parameters were used: Sample Number Lookup File: Q:\Cruise_Data\2004-08\ctd\hydro\addsamp.csv Bottle Channel Name: Bottle_Number -The following BINAVE parameters were used: Bin channel = Bottle_Number Averaging interval = 1.00 Minimum bin value = 0.000 Average value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -MERGE *** Secondary file not found *** Primary file : Q:\Cruise_Data\2004-08\ctd\hydro\2004-08-0029.samavg Secondary file: Q:\Cruise_Data\2004-08\ctd\hydro\2004-08-0029.sal -MERGE *** Secondary file not found *** Primary file : Q:\Cruise_Data\2004-08\CTD\hydro\2004-08-0029.mrg1 Secondary file: Q:\Cruise_Data\2004-08\CTD\hydro\2004-08-0029.add -The following CALIB parameters were used: Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = Q:\Cruise_Data\2004-08\ctd\doc\2004-08-recal1.ccf Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 11 Oxygen:Dissolved:SBE mL/L 10 0.6080000E-01 0.1015400E+01 CLEAN functions: - Interpolation by Record Number, in Pressure - Set event to last 4 characters of file name -The following SORT parameters were used. Sorted by channel: Pressure Pressure channel sorted according to values in sort channel -The following CHANNEL(S) were removed from file. Scan_Number Temperature:Secondary Conductivity:Primary Conductivity:Secondary Altimeter Salinity:T1:C1 Descent_Rate Oxygen:Voltage:SBE Status:Pump Flag -The following MERGE parameters were used: 2006/05/19 16:10:45 2006/05/19 16:10:45 Merge Channel: Sample_Number Merge Scheme Used: Interweave Records from Both Files Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Chlorophyll:Extracted, Flag:Chlorophyll:Extracted Primary file : Q:\Cruise_Data\2004-08\2004-08-0029.che1 Secondary file: Q:\Cruise_Data\2004-08\2004-08-0029.chcln -CLEAN functions: 2006/05/24 08:19:42 20 Reset #RECS, MIN & MAX values in header. Remove Sea-Bird comments from the header. Remove remark heading: "Comments from secondary" -CLEAN functions: 2018/07/06 08:58:27 20 Change Pad Value to -99 in All Channels. -SORT parameters: 2021/01/19 08:32:12 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/23 10:40:54 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-008_Nov2021\Processing\ IOS\2004-008-0029.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-008_Nov2021\Processing\ IOS\2004-008-0029.hplc -SORT parameters: 2021/11/23 10:41:01 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/23 10:41:08 20 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/23 10:41:35 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-008\Processing\Doc\ 2004-008-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 14: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 15: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/23 10:41:56 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-008\Processing\Doc\ 2004-008-bot-hdr2.txt $END *COMMENTS Analysis methods: ----------------- Salinity samples are collected in glass bottles and analyzed on Guildline model 8410 Portasal Salinometers which are standardized with IAPSO standard seawater. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965). A Brinkmann model 665 Dosimat and model PC910 Colorimeter is controlled by Visual-Basic program to titrate the oxygen samples. Nutrient samples are analyzed frozen using a Technicon AAII autoanalyzer following methods described in Barwell-Clarke and Whitney (1996). Chlorophyll samples are collected in duplicate in calibrated polycarbonate bottles (~330ml), then filtered onto 25mm MFS GF/75 filters at <70mmHg vacuum. The filters are placed in glass scintillation vials and immediately frozen at -20ēC until analysis. The chlorophyll is extracted in 10 ml of 90% acetone for 24h at -20ēC, then brought to room temperature and the extract is decanted and measured using a Turner Designs 10-AU-005-CE fluorometer, which has been calibrated with solutions of commercially prepared chlorophyll. HPLC samples were filtered onto 25mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 1-2 hours or for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~36-45 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, and Chlide-a. For further information see file QF 2004-008_HPLC*.xlsx. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. J. Barwell-Clarke and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 3. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Comments from secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-008_Nov2021\Processing\IOS\2004-008-0029. hplc --------------------------------------------------------------------------- Sample_Number 113: HPLC: >10%CV for TChl-a *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------------- ------ ---- ------ ------------ Oxygen:Dissolved:SBE mL/L 10 -99 () (0.608E-01 1.0154) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- --8--- ---9---- --10-- -11- --12-- 13 ---14-- ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- 36 !Samp Bott Pressu Temperat Trans Fluores PAR PAR: Salinity Oxygen Numb Chloro Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ re ure: missi cence: Refere :T0:C0 : er_o phyll: ag Chl-c3 Chlide- Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb Primary vity URU: nce Dissol ~bin Extrac ~a a a fuco fuco : !er er Seapoin ved: _rec ted ct HP ! t SBE ords ed LC !---- ---- ------ -------- ----- ------- ------ ------ -------- ------ ---- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 113 6 1.0 11.5369 16.6 11.221 21.6 184.5 25.9709 8.04 121 10.21 0 0.159 0.370 1.185 0.056 0.921 0.031 3.625 0.000 0.000 0.000 0.000 0.196 0.021 0.005 0.015 0.000 0.091 -99.000 7.752 0.400 0.104 8.122 36 112 5 4.9 10.6100 20.8 14.762 2.9 191.1 27.4558 7.53 118 13.89 0 0.253 0.505 1.594 0.055 1.317 0.027 5.089 0.000 0.000 0.007 0.000 0.252 0.024 0.015 0.017 0.000 0.078 -99.000 11.168 0.579 0.195 11.674 6 111 4 9.9 10.3207 21.0 14.661 0.8 197.6 27.5553 7.41 116 11.54 0 0.222 0.427 1.391 0.056 1.214 0.035 4.600 0.000 0.000 0.010 0.000 0.229 0.020 0.005 0.000 0.000 0.050 -99.000 10.052 0.598 0.171 10.479 6 110 3 14.9 9.8268 25.6 10.697 0.6 204.1 28.1157 6.65 121 7.24 0 0.123 0.164 0.825 0.032 0.502 0.031 3.039 0.000 0.000 0.015 0.000 0.156 0.007 0.000 0.000 0.000 0.040 -99.000 6.725 0.367 0.107 6.890 6 109 2 25.3 9.1063 27.5 2.235 0.5 219.9 29.3075 5.95 120 2.37 0 0.000 0.017 0.210 0.013 0.000 0.000 0.872 0.000 0.000 0.000 0.000 0.056 0.014 0.000 0.000 0.000 0.000 -99.000 2.243 0.000 0.037 2.260 6 108 1 36.0 8.9537 38.8 1.941 0.5 241.0 29.6744 5.58 120 1.90 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0