*2021/11/29 10:49:36.70 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2004/03/05 18:08:00.000 NUMBER OF RECORDS : 15 DATA DESCRIPTION : Bottle:Wire FILE TYPE : ASCII NUMBER OF CHANNELS : 36 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- -------- -------------- -------------- 1 Sample_Number n/a 271 285 2 Depth:Nominal metres 1 15 3 Nitrate_plus_Nitrite umol/L 10.2 27.6 4 Flag:Nitrate_plus_Nitrite n/a 5 Silicate umol/L 0 40.3 6 Flag:Silicate n/a 7 Phosphate umol/L 1.05 2.22 8 Flag:Phosphate n/a 9 Salinity:Bottle PSS-78 33.3988 33.3988 10 Flag:Salinity:Bottle n/a 11 Chlorophyll:Extracted mg/m^3 3.11 11.24 12 Flag:Chlorophyll:Extracted n/a 13 HPLC:Chl-c3 mg/m^3 0.134 0.145 14 HPLC:Chlide-a mg/m^3 0.111 0.135 15 HPLC:MgDVP mg/m^3 0.77E-01 0.125 16 HPLC:Chl-c2 mg/m^3 0.927 1.067 17 HPLC:Peri mg/m^3 0.44E-01 0.52E-01 18 HPLC:Pheide-a mg/m^3 0 0 19 HPLC:But-fuco mg/m^3 0 0 20 HPLC:Fuco mg/m^3 3.794 4.435 21 HPLC:Neo mg/m^3 0 0 22 HPLC:Pras mg/m^3 0 0 23 HPLC:Viola mg/m^3 0.5E-02 0.6E-02 24 HPLC:Hex-fuco mg/m^3 0 0.5E-02 25 HPLC:Diadino mg/m^3 0.237 0.287 26 HPLC:Allo mg/m^3 0.27E-01 0.34E-01 27 HPLC:Diato mg/m^3 0.3E-02 0.11E-01 28 HPLC:Zea mg/m^3 0 0 29 HPLC:Lut mg/m^3 0 0 30 HPLC:Chl-b mg/m^3 0.59E-01 0.6E-01 31 HPLC:DVChl-a mg/m^3 0 0 32 HPLC:Chl-a mg/m^3 9.047 10.075 33 HPLC:Phe mg/m^3 0.125 0.18 34 HPLC:B-Car mg/m^3 0.16 0.167 35 HPLC:TChl-a mg/m^3 9.157 10.21 36 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 6 F R4 0 3 -99 ' ' 6 F R4 1 4 -99 ' ' 3 NQ C ' ' 5 -99 ' ' 6 F R4 1 6 -99 ' ' 3 NQ C ' ' 7 -99 ' ' 7 F R4 2 8 -99 ' ' 3 NQ C ' ' 9 -99 ' ' 9 F R4 4 10 -99 ' ' 3 NQ C ' ' 11 -99 ' ' 7 F R4 2 12 -99 ' ' 3 NQ C ' ' 13 -99 ' ' 8 F R4 3 14 -99 ' ' 8 F R4 3 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 3 NQ C ' ' $END $REMARKS Flag channels initialized with zeros. Non-zero values have the following significance: -------------------------------------------------------------------------------------- 1 = Sample for this measurement was drawn from water bottle but not analyzed (not normally used). 2 = Acceptable measurement (not normally used). 3 = Questionable measurement (no problem observed in sampling or analysis, but value is not trusted, nonetheless; includes outlyers). 4 = Bad measurement (known problem with sampling or analysis, but not serious enough to completely discard the value). 5 = Not reported (lost sample; unredeemably bad measurement). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample not drawn for this measurement from this bottle (not normally used). -------------------------------------------------------------------------------------- $END *ADMINISTRATION MISSION : 2004-04 AGENCY : IOS, Ocean Science and Productivity, Sidney, B.C. COUNTRY : Canada PROJECT : Bio_Moorings SCIENTIST : Pena A. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : WCVI/Strait of Georgia STATION : SI03 EVENT NUMBER : 46 LATITUDE : 48 35.60000 N ! (deg min) LONGITUDE : 123 29.97000 W ! (deg min) WATER DEPTH : 223 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0585 $TABLE: SENSORS ! Name Abs Depth Serial No ! ------------------------- -------------- ---------- temperature:primary ' ' 2710 conductivity:primary ' ' 1763 pressure ' ' 77511 temperature:secondary ' ' 2095 conductivity:secondary ' ' 1764 Fluorometer ' ' Seapoint Oxygen:SBE ' ' 0047 transmissometer:primary ' ' 333DR altimeter: ' ' ' ' irradiance ' ' primary surface ' ' 'degrees = 0.0' $END *COMMENTS Comments from secondary file: Q:\Cruise_Data\2004-04\hydro\2004-04-0002.chl --------------------------------------------- Sample #5 & 6: Variability of duplicates >10% Analysis methods: ----------------- Salinity samples are collected in glass bottles and analyzed on Guildline model 8410 Portasal Salinometers which are standardized with IAPSO standard seawater. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965). A Brinkmann model 665 Dosimat and model PC910 Colorimeter is controlled by Visual-Basic program to titrate the oxygen samples. Nutrient samples were stored frozen and analysed at IOS. Nutrient samples are analyzed frozen using a Technicon AAII autoanalyzer following methods described in Barwell-Clarke and Whitney (1996). Chlorophyll samples are collected in duplicate in calibrated polycarbonate bottles (~330ml), then filtered onto 25mm MFS GF/75 filters at <70mmHg vacuum. The filters are placed in glass scintillation vials and immediately frozen at -20ēC until analysis. The chlorophyll is extracted in 10 ml of 90% acetone for 24h at -20ēC, then brought to room temperature and the extract is decanted and measured using a Turner Designs 10-AU-005-CE fluorometer, which has been calibrated with solutions of commercially prepared chlorophyll. HPLC samples were filtered onto 25mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~4 years after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, and Chlide-a. For further information see file QF 2004-004_HPLC*.xlsx. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. J. Barwell-Clarke and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 3. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. -------------------------------------------------------------------------- The CTD data for this cruise were lost. Depths were taken from the rosette sheet so are considered estimates and named Depth:Nominal. -------------------------------------------------------------------------- *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! -------- ------ ---------- -------- --------- --------- MERGE 3.5.1 2021/01/07 09:42:19 16 15 CLEAN 5.2.4 2021/01/07 09:42:28 15 15 HDREDIT2 3.2 2021/01/07 10:24:49 ? ? HDREDIT2 3.2 2021/01/07 10:26:01 ? ? HDREDIT2 3.2 2021/11/29 10:49:36 ? ? $END $REMARKS -The following MERGE parameters were used: 2021/01/07 09:42:19 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: Nitrate_plus_Nitrite [ ], Flag:Nitrate_plus_Nitrite [ ], Silicate [ ], Flag:Silicate [ ], Phosphate [ ], Flag:Phosphate [ ], Salinity:Bottle [ ], Flag:Salinity:Bottle [ ], Chlorophyll:Extracted [ ], Flag:Chlorophyll:Extracted [ ], HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Gyr-de [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-004\Processing\IOS\2004- 004-0046.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-004\Processing\IOS\2004- 004-0046.hplc -CLEAN functions: 2021/01/07 09:42:28 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/01/07 10:24:49 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-004\Processing\Doc\ 2004-004-bot-hdr1.txt Channel 13: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 14: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 15: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 35: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/01/07 10:26:01 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2004\2004-004\Processing\Doc\ 2004-004-bot-hdr2.txt -HEADER EDITS: 2021/11/29 10:49:36 Channel 3: Nitrate_plus_Nitrite [umol/L] Format: F7.2 ==> F6.1 Channel 5: Silicate [umol/L] Format: F7.2 ==> F6.1 Channel 7: Phosphate [umol/L] Format: F8.3 ==> F7.2 $END !-1-- --2-- --3-- 4- --5-- 6- --7--- 8- ---9---- 10 --11-- 12 ---13-- ---14-- ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- 36 !Samp Depth Nitra Fl Silic Fl Phosph Fl Salinity Fl Chloro Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ : te_ ag ate ag ate ag :Bottle ag phyll: ag Chl-c3 Chlide- MgDVP Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Nomin plus_ ~t ~i ~p ~o Extrac ~a a a fuco fuco : !er al Nitri ri ca ha tt ted ct HP ! te te te te le ed LC !---- ----- ----- -- ----- -- ------ -- -------- -- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 271 1. 27.6 0 39.2 0 2.18 0 33.3988 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 272 2. 27.2 0 39.0 0 2.22 0 -99.0000 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 273 3. 24.8 0 36.3 0 2.13 0 -99.0000 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 274 4. 24.8 0 37.9 0 1.96 0 -99.0000 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 275 5. 25.2 0 40.3 0 2.07 0 -99.0000 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 276 6. 24.9 0 40.2 0 2.07 0 -99.0000 0 -99.00 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 277 7. 20.7 0 33.3 0 1.81 0 -99.0000 0 3.11 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 278 8. 16.9 0 26.7 0 1.56 0 -99.0000 0 5.02 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 279 9. 10.9 0 15.3 0 1.14 0 -99.0000 0 10.50 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 280 10. -99.0 0 -99.0 0 -99.00 0 -99.0000 0 9.74 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 281 11. 10.4 0 15.0 0 1.05 0 -99.0000 0 -99.00 0 0.134 0.111 0.077 0.927 0.044 0.000 0.000 3.794 0.000 0.000 0.005 0.000 0.237 0.027 0.003 0.000 0.000 0.059 0.000 9.047 0.180 0.160 9.157 6 282 12. 10.2 0 0.0 0 1.06 0 -99.0000 0 11.24 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 283 13. -99.0 0 -99.0 0 -99.00 0 -99.0000 0 9.76 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 284 14. -99.0 0 -99.0 0 -99.00 0 -99.0000 0 -99.00 0 0.145 0.135 0.125 1.067 0.052 0.000 0.000 4.435 0.000 0.000 0.006 0.005 0.287 0.034 0.011 0.000 0.000 0.060 0.000 10.075 0.125 0.167 10.210 6 285 15. -99.0 0 -99.0 0 -99.00 0 -99.0000 0 10.20 0 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0