*2021/11/23 09:24:31.63 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2000/03/16 15:18:40.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 12 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII NUMBER OF CHANNELS : 35 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- --------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 1 12 2 Bottle_Number n/a 1 12 3 Pressure decibar 1.7 82.4 4 Temperature:Primary 'deg C (ITS90)' 8.7389 8.7606 5 Transmissivity %/metre 28 61.4 6 Fluorescence:URU:Seapoint mg/m^3 0.26 0.595 7 PAR uE/m^2/sec 2.1 129.8 8 Salinity:T0:C0 PSS-78 32.6124 32.7421 9 Nitrate_plus_Nitrite umol/L 4.8 7.7 10 Silicate umol/L 9.9 12.7 11 Phosphate umol/L 0.77 0.92 12 HPLC:Chl-c3 mg/m^3 0.17E-01 0.44E-01 13 HPLC:Chlide-a mg/m^3 0 0 14 HPLC:MgDVP mg/m^3 0.6E-02 0.17E-01 15 HPLC:Chl-c2 mg/m^3 0.14E-01 0.55E-01 16 HPLC:Peri mg/m^3 0 0 17 HPLC:Pheide-a mg/m^3 0 0 18 HPLC:But-fuco mg/m^3 0.16E-01 0.32E-01 19 HPLC:Fuco mg/m^3 0.21E-01 0.32E-01 20 HPLC:Neo mg/m^3 0 0.6E-02 21 HPLC:Pras mg/m^3 0 0.16E-01 22 HPLC:Viola mg/m^3 0 0 23 HPLC:Hex-fuco mg/m^3 0.26E-01 0.69E-01 24 HPLC:Diadino mg/m^3 0 0.8E-02 25 HPLC:Allo mg/m^3 0 0.27E-01 26 HPLC:Diato mg/m^3 0 0 27 HPLC:Zea mg/m^3 0.7E-02 0.13E-01 28 HPLC:Lut mg/m^3 0 0 29 HPLC:Chl-b mg/m^3 0.54E-01 0.107 30 HPLC:DVChl-a mg/m^3 -99 -99 31 HPLC:Chl-a mg/m^3 0.22 0.535 32 HPLC:Phe mg/m^3 0 0 33 HPLC:B-Car mg/m^3 0 0.8E-02 34 HPLC:TChl-a mg/m^3 0.22 0.535 35 Flag:HPLC ' ' $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 7 F ' ' 1 4 -99 ' ' 9 F ' ' 4 5 -99 ' ' 6 F ' ' 1 6 -99 ' ' 8 F ' ' 3 7 -99 ' ' 7 F ' ' 1 8 -99 ' ' 9 F ' ' 4 9 -99 ' ' 6 F R4 1 10 -99 ' ' 6 F R4 1 11 -99 ' ' 7 F R4 2 12 -99 ' ' 8 F R4 3 13 -99 ' ' 8 F R4 3 14 -99 ' ' 8 F R4 3 15 -99 ' ' 8 F R4 3 16 -99 ' ' 8 F R4 3 17 -99 ' ' 8 F R4 3 18 -99 ' ' 8 F R4 3 19 -99 ' ' 8 F R4 3 20 -99 ' ' 8 F R4 3 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 3 NQ C ' ' $END $REMARKS This file converted from Sea-Bird file: C:\DATA\2000-03\20030001.ROS $END *ADMINISTRATION MISSION : 2000-03 AGENCY : 'IOS, Ocean Science and Productivity Division,' CONTINUED : ' Sidney, B.C.' COUNTRY : Canada PROJECT : Laperouse, Line P SCIENTIST : Pena A. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : West Coast, station MP04 STATION : P4 EVENT NUMBER : 1 LATITUDE : 48 39.10000 N ! (deg min) LONGITUDE : 126 40.20000 W ! (deg min) WATER DEPTH : 1324 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE 911plus SERIAL NUMBER : 0550 $TABLE: SENSORS ! Name Abs Depth Serial No ! ----------------- -------------- ---------- temperature ' ' primary conductivity ' ' primary pressure ' ' 75636 temperature ' ' secondary conductivity ' ' secondary Seapoint ' ' ' ' transmissometer ' ' 192D irradiance ' ' 4565 $END $REMARKS SOFTWARE VERSION 4.243 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SBE_IOS 1.1 2000/03/17 20:42:24 1164 1164 DESPIKE 1.4 2000/03/17 20:53:34 1164 1164 BINAVE 3.0 2000/03/18 18:53:55 1164 12 REMOVECH 3.1 2000/03/18 12:48:45 12 12 REMOVECH 5.0 2000/10/23 16:01:02 12 12 CALIB 9.0 2000/11/17 09:40:27 12 12 REMOVECH 5.0 2000/11/17 10:32:10 12 12 MERGE 2.6 2001/02/28 08:37:53 12 12 CALIB 10.2 2002/01/21 16:22:58 12 12 REMOVECH 6.0 2004/01/19 12:22:21 12 12 HDREDIT2 1.9 2004/01/19 13:54:45 ? ? SORT 3.4.1 2004/01/19 13:55:40 12 12 CLEAN 4.5 2004/01/19 13:55:47 12 12 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 15:20:37 12 12 SORT 3.6 2021/01/12 14:38:56 12 12 REORDER 1.3.1 2021/11/23 09:22:44 ? ? MERGE 3.5.1 2021/11/23 09:23:10 12 12 SORT 3.6 2021/11/23 09:23:18 12 12 CLEAN 5.2.4 2021/11/23 09:23:28 12 12 HDREDIT2 3.2 2021/11/23 09:24:09 ? ? HDREDIT2 3.2 2021/11/23 09:24:31 ? ? $END $REMARKS -The following DESPIKE parameters were used: DESPIKE TABLE CHANNEL FIT OVER- MIN MAX MIN MAX SPIKE WIDTH LAP VALUE VALUE STDDEV STDDEV TOL. -------------------- ----- ----- ------- ------- ------- ------- ----- PR 55 5 .00 2000.00 .10000 999.000 3.00 Temperature:Primary 55 5 2.00 20.00 .00100 999.000 3.00 Temperature:Secondar 55 5 2.00 20.00 .00100 999.000 3.00 Salinity:T0:C0 55 5 20.00 35.00 .00100 999.000 3.00 Salinity:T1:C1 55 5 20.00 35.00 .00100 999.000 3.00 -The following BINAVE parameters were used: Bin channel = BOTTLE_NUMBER Averaging interval = 1.0 Minimum bin value = 0.000 Bin value was used Interpolated values were NOT used for empty bins Channel 'NUMBER_OF_BIN_RECORDS' was added to file. -The following CHANNEL(S) were removed from file. Scan_Number flag -The following CHANNEL(S) were removed from file. Conductivity:Primary Conductivity:Secondary -The following CALIB parameters were used: Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Pressure offset = 0.0 Calibration file = d:\data\2000\2000-03\salcor.txt Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 3 Salinity:T0:C0 PSS-78 10 0.7000000E-02 0.1000000E+01 5 Salinity:T1:C1 PSS-78 10 0.2100000E-01 0.1000000E+01 -The following CHANNEL(S) were removed from file. Temperature:Secondary Salinity:T1:C1 Number_of_bin_records -The following MERGE parameters were used: Merge Channel: Sample_Number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: Pressure, Temperature:Primary, Salinity:T0:C0, Transmissivity, Chl_fluorescence:Seapoint, Irradiance_PAR, Bottle_Number, Sample_Number Secondary Channels to Include: Nitrate, Silicate, Phosphate Primary file : d:\data\2000\2000-03\20030001.HYD Secondary file: d:\data\2000\2000-03\20030001.NUT -The following CALIB parameters were used: Calibration type = Correct Mode: ONLY - calibration specs from Cal File only. Calibration file = M:\data\fluor\pena\2000-03\2003par.CCF Calibrations applied: Ch Name Units Fmla Coefficents -- ----------------------------- --------- --- ----------------------------- 7 Irradiance_PAR n/a 10 0.0000000E+00 0.1000000E+00 -The following CHANNEL(S) were removed from file. Sample_Number -The following SORT parameters were used. Sorted by channel: Pressure Pressure channel sorted according to values in sort channel CLEAN functions: - Reset #RECS, MIN & MAX values in header. - Change Pad Value to -99 in All Channels. - Delete Empty Channels: 0 deleted. - Set channel name post-colon letters to upper case. - Remove Sea-Bird comments from the header. -SORT parameters: 2021/01/12 14:38:56 Sorted in ascending order of channel Sample_Number [n/a] -The following MERGE parameters were used: 2021/11/23 09:23:10 Merge Channel: sample_number Merge Scheme Used: Add Secondary to Primary Overlap Scheme Used: Keep Primary Primary Channels to Include: ALL Secondary Channels to Include: HPLC:Chl-c3 [ ], HPLC:Chlide-a [ ], HPLC:MgDVP [ ], HPLC:Chl-c2 [ ], HPLC:Chl-c1 [ ], HPLC:Me-chlide [ ], HPLC:Peri [ ], HPLC:Pheide-a [ ], HPLC:But-fuco [ ], HPLC:Fuco [ ], HPLC:Neo [ ], HPLC:Pras [ ], HPLC:Viola [ ], HPLC:Hex-fuco [ ], HPLC:Diadino [ ], HPLC:Allo [ ], HPLC:Diato [ ], HPLC:Zea [ ], HPLC:Lut [ ], HPLC:Chl-b [ ], HPLC:C2mgdg, HPLC:DVChl-a [ ], HPLC:Chl-a [ ], HPLC:Phe [ ], HPLC:B-Car [ ], HPLC:TChl-a [ ], Flag:HPLC [ ] Primary file : C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2000\2000-003_Nov2021\Processing\ ios\2000-003-0001.che2 Secondary file: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2000\2000-003_Nov2021\Processing\ ios\2000-003-0001.hplc -SORT parameters: 2021/11/23 09:23:18 Sorted in ascending order of channel Pressure [decibar] -CLEAN functions: 2021/11/23 09:23:28 Reset #RECS, MIN & MAX values in header. Change Pad Value to -99 in All Channels. Change character data from " " to "0" in channels Flag:* -HEADER EDITS: 2021/11/23 09:24:09 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2000\2000-003\Processing\Doc\ 2000-003-bot-hdr1.txt Channel 2: Bottle_Number [n/a] Format: I3 ==> I5 Channel 12: HPLC:Chl-c3 [mg/m^3] Units: ==> mg/m^3 Channel 13: HPLC:Chlide-a [mg/m^3] Units: ==> mg/m^3 Channel 14: HPLC:MgDVP [mg/m^3] Units: ==> mg/m^3 Channel 15: HPLC:Chl-c2 [mg/m^3] Units: ==> mg/m^3 Channel 16: HPLC:Peri [mg/m^3] Units: ==> mg/m^3 Channel 17: HPLC:Pheide-a [mg/m^3] Units: ==> mg/m^3 Channel 18: HPLC:But-fuco [mg/m^3] Units: ==> mg/m^3 Channel 19: HPLC:Fuco [mg/m^3] Units: ==> mg/m^3 Channel 20: HPLC:Neo [mg/m^3] Units: ==> mg/m^3 Channel 21: HPLC:Pras [mg/m^3] Units: ==> mg/m^3 Channel 22: HPLC:Viola [mg/m^3] Units: ==> mg/m^3 Channel 23: HPLC:Hex-fuco [mg/m^3] Units: ==> mg/m^3 Channel 24: HPLC:Diadino [mg/m^3] Units: ==> mg/m^3 Channel 25: HPLC:Allo [mg/m^3] Units: ==> mg/m^3 Channel 26: HPLC:Diato [mg/m^3] Units: ==> mg/m^3 Channel 27: HPLC:Zea [mg/m^3] Units: ==> mg/m^3 Channel 28: HPLC:Lut [mg/m^3] Units: ==> mg/m^3 Channel 29: HPLC:Chl-b [mg/m^3] Units: ==> mg/m^3 Channel 30: HPLC:DVChl-a [mg/m^3] Units: ==> mg/m^3 Channel 31: HPLC:Chl-a [mg/m^3] Units: ==> mg/m^3 Channel 32: HPLC:Phe [mg/m^3] Units: ==> mg/m^3 Channel 33: HPLC:B-Car [mg/m^3] Units: ==> mg/m^3 Channel 34: HPLC:TChl-a [mg/m^3] Units: ==> mg/m^3 -HEADER EDITS: 2021/11/23 09:24:31 Applied edit header: C:\Users\Samantha\OneDrive\Documents\SH ios\HPLC\2000\2000-003\Processing\Doc\ 2000-003-bot-hdr2.txt $END *COMMENTS Collection method: ------------------ A rosette, holding 24 ten-litre water samplers, with a CTD was used. Analysis methods: ----------------- Salinity samples are collected in glass bottles and analyzed on Guildline model 8410 Portasal Salinometers which are standardized with IAPSO standard seawater. Oxygen samples are analyzed on an automated Winkler titration system following the procedures of Carpenter (1965). A Brinkmann model 665 Dosimat and model PC910 Colorimeter is controlled by Visual-Basic program to titrate the oxygen samples. Nutrient samples are analyzed frozen using a Technicon AAII autoanalyzer following methods described in Barwell-Clarke and Whitney (1996). Chlorophyll samples are collected in duplicate in calibrated polycarbonate bottles (~330ml), then filtered onto 25mm MFS GF/75 filters at <70mmHg vacuum. The filters are placed in glass scintillation vials and immediately frozen at -20ēC until analysis. The chlorophyll is extracted in 10 ml of 90% acetone for 24h at -20ēC, then brought to room temperature and the extract is decanted and measured using a Turner Designs 10-AU-005-CE fluorometer, which has been calibrated with solutions of commercially prepared chlorophyll. HPLC samples were filtered onto 25mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours in the lab and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~7 years after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, and Chlide-a. For further information see file QF 2000-003_HPLC*.xlsx. References: 1. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 2. J. Barwell-Clarke and F. Whitney. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 3. Nemcek, N. and Peņa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. *CALIBRATION $TABLE: CORRECTED CHANNELS ! Name Units Fmla Pad Coefficients ! ---------------- -------- ---- ------ ------------ Salinity:T0:C0 PSS-78 10 -9.99 () (0.7E-02 1) Salinity:T1:C1 PSS-78 10 -9.99 () (0.21E-01 1) Irradiance_PAR n/a 10 -9.99 () (0 0.1) $END !-1-- -2-- --3--- ---4---- --5-- ---6--- --7--- ---8---- --9-- --10- --11-- ---12-- ---13-- ---14-- ---15-- ---16-- ---17-- ---18-- ---19-- ---20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- 35 !Samp Bott Pressu Temperat Trans Fluores PAR Salinity Nitra Silic Phosph HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ re ure: missi cence: :T0:C0 te_ ate ate Chl-c3 Chlide- MgDVP Chl-c2 Peri Pheide- But- Fuco Neo Pras Viola Hex- Diadino Allo Diato Zea Lut Chl-b DVChl-a Chl-a Phe Car TChl-a ag !Numb Numb Primary vity URU: plus_ a a fuco fuco : !er er Seapoin Nitri HP ! t te LC !---- ---- ------ -------- ----- ------- ------ -------- ----- ----- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 12 12 1.7 8.7430 31.1 0.578 87.0 32.6124 -99.0 -99.0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 11 11 1.9 8.7441 28.0 0.556 129.8 32.6148 -99.0 -99.0 -99.00 0.029 0.000 0.014 0.039 0.000 0.000 0.026 0.030 0.000 0.010 0.000 0.066 0.000 0.020 0.000 0.011 0.000 0.082 -99.000 0.433 0.000 0.000 0.433 0 10 10 20.6 8.7452 58.6 0.592 12.5 32.6211 -99.0 -99.0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 9 9 20.7 8.7389 58.6 0.595 12.9 32.6202 -99.0 -99.0 -99.00 0.037 0.000 0.017 0.055 0.000 0.000 0.028 0.027 0.000 0.011 0.000 0.060 0.000 0.025 0.000 0.012 0.000 0.084 -99.000 0.515 0.000 0.000 0.515 0 7 7 29.3 8.7462 58.6 0.589 8.2 32.6216 4.8 9.9 0.79 0.022 0.000 0.016 0.043 0.000 0.000 0.030 0.030 0.006 0.009 0.000 0.057 0.000 0.020 0.000 0.013 0.000 0.079 -99.000 0.472 0.000 0.000 0.472 0 8 8 30.2 8.7451 58.7 0.507 7.8 32.6209 5.2 10.4 0.78 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 5 5 49.3 8.7498 58.5 0.587 3.6 32.6210 5.1 10.2 0.78 0.030 0.000 0.011 0.043 0.000 0.000 0.024 0.029 0.000 0.016 0.000 0.067 0.000 0.027 0.000 0.012 0.000 0.095 -99.000 0.535 0.000 0.008 0.535 0 6 6 49.8 8.7515 58.5 0.542 3.5 32.6216 5.2 10.4 0.79 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 4 4 65.7 8.7497 58.5 0.559 2.5 32.6205 5.3 10.4 0.77 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 3 3 66.2 8.7493 58.4 0.534 2.5 32.6204 5.1 10.4 0.78 0.044 0.000 0.013 0.053 0.000 0.000 0.032 0.032 0.000 0.011 0.000 0.069 0.008 0.024 0.000 0.011 0.000 0.107 -99.000 0.498 0.000 0.008 0.498 0 2 2 82.2 8.7601 61.4 0.260 2.1 32.7411 7.2 12.2 0.87 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 1 1 82.4 8.7606 61.4 0.263 2.1 32.7421 7.7 12.7 0.92 0.017 0.000 0.006 0.014 0.000 0.000 0.016 0.021 0.000 0.000 0.000 0.026 0.000 0.000 0.000 0.007 0.000 0.054 -99.000 0.220 0.000 0.000 0.220 0