*2025/05/29 08:53:44.55 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 1977/10/17 00:00:00.000 NUMBER OF RECORDS : 6 DATA DESCRIPTION : Bottle FILE TYPE : ASCII NUMBER OF CHANNELS : 10 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ---------------------------- ---------- -------------- -------------- 1 Depth metres 0 20 2 Temperature 'deg C' 10.4 11 3 Salinity ppt 25.8 29.5 4 Oxygen:Dissolved mL/L 4.46 6.48 5 Nitrate umol/L 8.6 21.4 6 Phosphate umol/L 1.5 2.1 7 Silicate umol/L 28.6 32.1 8 Chlorophyll:Extracted mg/m^3 0.52 3.53 9 Carbon:Dissolved:Inorganic mg/L 22 23.4 10 Phytoplankton:Volume mm^3/m^3 396 1788 $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 6 F R4 1 2 -99 ' ' 6 F R4 1 3 -99 ' ' 6 F R4 1 4 -99 ' ' 7 F R4 2 5 -99 ' ' 6 F R4 1 6 -99 ' ' 6 F R4 1 7 -99 ' ' 6 F R4 1 8 -99 ' ' 7 F R4 2 9 -99 ' ' 6 F R4 1 10 -99 ' ' 7 F R4 0 $END $REMARKS Time of sampling is not known. $END *ADMINISTRATION MISSION : 1977-99 PROJECT : Phytoplankton Ecology of the Strait of Georgia SCIENTIST : Stockner J.G. PLATFORM : Vector *LOCATION STATION : 9 EVENT NUMBER : 103 LATITUDE : 49 9.00000 N ! (deg min) LONGITUDE : 123 15.00000 W ! (deg min) SECCHI (M) : 4 ! custom item MEC : ! custom item PRIMARY PRODUCTION : 1122 ! custom item ZOOPLANKTON BIOMASS : 8.7 ! custom item STATIONINDEX NUMBER : 290 ! custom item $REMARKS StationIndex Number is the same as the StationIndex number from the original Access database, STOCKNER.MDB. Secchi units = metres Primary Production = mgC/m^^2/d Zooplankton Biomass units = mg dry weight/m^^3 $END *HISTORY $TABLE: PROGRAMS ! Name Vers Date Time Recs In Recs Out ! ------------------------ ------ ---------- -------- --------- --------- SPRD2IS 4.3 2005/04/22 10:41:52 6 6 HDREDIT2 2.4 2005/04/22 10:53:06 ? ? CLEAN 4.7.1 2005/04/22 11:03:56 6 6 CHANGE_CTD_CHANNEL_NAMES 1.0 2013/12/16 14:16:03 6 6 HDREDIT2 3.1.1 2019/12/13 13:42:22 ? ? HDREDIT2 3.2 2025/05/29 08:53:44 ? ? $END $REMARKS -HEADER EDITS: 2005/04/22 10:53:06 Applied edit header: C:\Temp\Stockner\header.TXT -CLEAN functions: 2005/04/22 11:03:49 20 Reset #RECS, MIN & MAX values in header. Delete Empty Channels: 0 deleted. Set event to last 4 characters of file name -HEADER EDITS: 2019/12/13 13:42:22 - none. -HEADER EDITS: 2025/05/29 08:53:44 Channel 10: Phytoplankton:Volume [mm^3/m^3] Format: F6.0 ==> F7.0 $END *COMMENTS These files were acquired by Robin Brown. Materials and Methods --------------------- Total incident solar radiation was recorded on a Belfort pyrheliometer mounted on board ship, and extinction of surface light with depth was measured by a Montedoro-Whitney Underwater Illuminance meter (Model LMT-8A). The natural logarithm of light intensity was plotted against depth, and the slope of a straight line fitted to the points by, linear regression provided an estimate of the mean downward extinction coefficient (k). A standard 30-cm white Secchi disc was used to measure water transparency at every station. Temperature profiles to a depth of 50 m were obtained with a bathythermograph and surface temperatures were measured with a bucket thermometer. Water samples were collected from 1, 3, 5, and 20 m depths, and later analyzed for carbonate alkalinity, salinity, dissolved oxygen, nitrate (NO3-1), total phosphate (TPO4-3), and silicate (SiO4-4). The method of Strickland and Parsons (1968) was used to determine carbonate alkalinity, using an Orion digital pH meter (Model 801). However, if after acid titration final pH values fell below 3.00, then the sample analysis was repeated using the APHA et al. (1971) standard titrimetric method. Salinity was analyzed with an Auto-lab salinometer (Model 601) to an accuracy of ±0.5‰. Density was computed by nomograph from temperature and salinity records. Dissolved oxygen (samples fixed within an hour of being taken) was determined by use of the Winkler titration method (Strickland and Parsons 1968). Samples for nutrient analysis were immediately frozen and later analyzed by the Environmental Protection Service analytical laboratory, Pacific Environment Institute, West Vancouver, using methods outlined in their manual (Fisheries/EPS 1974). The standard 14C method as initially proposed by Steemann-Nielsen (1952) was used, with some modifications. Water was collected from surface skim, and 0.5, 1, 2, 3, 5, 10, 20, and 30 m depths using a 6-L polyvinyl chloride Van Dorn bottle. Productivity bottles (two 125 mL light and one 125 mL dark) were inoculated with 1 mL NaH14CO3. (37kBq.mL-1). For each experiment, the number of disintegrations.min-l (dpm).mL-l was determined by placing 1 mL in each of three scintillation vials. In most cases, samples were incubated in situ for 4-5 h, normally from 0930 to 1430. Water was filtered through 0.45 µm BDH cellulose nitrate filters and placed in 10 mL of a specially prepared toluene-based fluor (POPOP, PPO, 2-ethoxyethanol, and toluene). Samples were analyzed within 24 h for activity in a Packard Tri-Carb Liquid Scintillation Spectrometer (Model 3375). The equation of Strickland (1960) was used to convert dpms to mg C. m-3. Using incident solar radiation for the entire light day and for the incubation period, data were converted to mg C. m-3 . d-l and integrated by a Hewlett-Packard Calculator and Plotter (Model 9820 A, 9862 A) to give phytoplankton production on an area basis (mg C.m-2.d-1). This involved linear interpolation between data points to the depths where light and dark uptake were equal. Detailed examination was made of phytoplankton samples from only 1 and 5 m depths to assess (a) species composition and (b) phytoplankton volumes during key spring and fall bloom periods. Phytoplankton samples were preserved in Lugol's, acetate solution and enumerated, usmg the Utermöhl (1958) sedimentation method. Volumes were determined by equating phytop1ankton cells to known geo-metric shapes. Each phytoplankton sample was examined under 175 and 700X magnification using a Wild M40 inverted plankton microscope. Results were expressed as cells and totaI volume. m-3. One liter of sea water was taken for chlorophyll a analysis from I, 3, 5, and 20 m depths and filtered onto 5.5-cm Whatman GF/C glass fiber filters with a small amount of MgCO3 added. Filters were macerated in a tissue grinder with 10 mL of 90% acetone, and the filtrate analyzed on a Carr recording spectrophotometer (Model 15). The equation of Strickland and Parsons (1968) was used to calculate chlorophyll a. Vertical zooplankton hauls from 50 m were made at each station with a SCOR-UNESCO plankton net, with mouth diameter of 57 cm and screen mesh size of 350 µm. Samples were preserved in 5% formaldehyde, returned to the laboratory, and split into two equal subsamples, One portion was dried overnight at 90ºC, and results expressed as zooplankton biomass in mg (dry weight) .m-3. The other portion was preserved for future reference. Reference: ---------- Stockner, J.G.,D.D. Cliff and K.S. Shorttreed. 1979. Phytoplankton ecology of the Strait of Georgia, British Columbia. J. Fish. Res. Board Can. 36: 657-666. !--1-- --2-- --3-- --4--- --5-- --6-- --7-- --8--- --9-- --10-- !Depth Tempe Salin Oxygen Nitra Phosp Silic Chloro Carbo Phytop ! ratur ity : te hate ate phyll: n:Dis lankto ! e Dissol Extrac ~lved n: ! ved ted :Inor Volume ! ganic !----- ----- ----- ------ ----- ----- ----- ------ ----- ------ *END OF HEADER 0.0 11.0 -99.0 -99.00 -99.0 -99.0 -99.0 -99.00 -99.0 -99. 1.0 10.9 25.8 6.08 8.6 1.5 28.6 3.24 22.1 881. 3.0 10.8 26.7 6.48 -99.0 -99.0 -99.0 3.53 22.0 1788. 5.0 10.8 28.9 5.00 16.4 2.0 32.1 1.20 23.0 803. 10.0 -99.0 -99.0 -99.00 -99.0 -99.0 -99.0 -99.00 -99.0 396. 20.0 10.4 29.5 4.46 21.4 2.1 32.1 0.52 23.4 -99.