*2023/02/17 06:29:49.85 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2017/02/16 19:18:10.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : F34D6788 NUMBER OF CHANNELS : 49 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 428 428 2 Bottle_Number n/a 1 1 3 Bottle:Firing_Sequence n/a 1 1 4 Pressure:CTD dbar 5.7 5.7 5 Temperature:CTD deg_C_(ITS90) 7.6413 7.6413 6 Salinity:CTD PSS-78 32.6082 32.6082 7 Sigma-t:CTD kg/m^3 25.4729 25.4729 8 Oxygen:Dissolved:CTD:Volume ml/l 6.66 6.66 9 Oxygen:Dissolved:CTD:Mass µmol/kg 290 290 10 Fluorescence:CTD:Seapoint mg/m^3 0.483 0.483 11 PAR:CTD µE/m^2/sec 176.3 176.3 12 Salinity:Bottle PSS-78 32.6119 32.6119 13 Flag:Salinity:Bottle n/a 14 Nitrate_plus_Nitrite:Bottle µmol/l 7.9 7.9 15 Flag:Nitrate_plus_Nitrite:Bottle n/a 16 Phosphate:Bottle µmol/l 0.861 0.861 17 Flag:Phosphate:Bottle n/a 18 Silicate:Bottle µmol/l 9.06 9.06 19 Flag:Silicate:Bottle n/a 20 Chlorophyll:Extracted:Bottle mg/m^3 0.4 0.4 21 Flag:Chlorophyll:Extracted:Bottle n/a 22 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.15 0.15 23 HPLC:Chl-c3:Bottle mg/m^3 0.59E-01 0.59E-01 24 HPLC:Chlide-a:Bottle mg/m^3 0 0 25 HPLC:MgDVP:Bottle mg/m^3 0.3E-02 0.3E-02 26 HPLC:Chl-c2:Bottle mg/m^3 0.44E-01 0.44E-01 27 HPLC:Chl-c1:Bottle mg/m^3 0 0 28 HPLC:Me-chlide:Bottle mg/m^3 0 0 29 HPLC:Peri:Bottle mg/m^3 0 0 30 HPLC:Pheide-a:Bottle mg/m^3 0 0 31 HPLC:But-fuco:Bottle mg/m^3 0.45E-01 0.45E-01 32 HPLC:Fuco:Bottle mg/m^3 0.35E-01 0.35E-01 33 HPLC:Neo:Bottle mg/m^3 0.2E-02 0.2E-02 34 HPLC:Pras:Bottle mg/m^3 0.3E-02 0.3E-02 35 HPLC:Viola:Bottle mg/m^3 0 0 36 HPLC:Hex-fuco:Bottle mg/m^3 0.161 0.161 37 HPLC:Diadino:Bottle mg/m^3 0.24E-01 0.24E-01 38 HPLC:Allo:Bottle mg/m^3 0.16E-01 0.16E-01 39 HPLC:Diato:Bottle mg/m^3 0.12E-01 0.12E-01 40 HPLC:Zea:Bottle mg/m^3 0.7E-02 0.7E-02 41 HPLC:Lut:Bottle mg/m^3 0.4E-02 0.4E-02 42 HPLC:Chl-b:Bottle mg/m^3 0.51E-01 0.51E-01 43 HPLC:C2mgdg:Bottle mg/m^3 0.14E-01 0.14E-01 44 HPLC:DVChl-a:Bottle mg/m^3 0 0 45 HPLC:Chl-a:Bottle mg/m^3 0.413 0.413 46 HPLC:Phe:Bottle mg/m^3 0 0 47 HPLC:B-Car:Bottle mg/m^3 0.4E-02 0.4E-02 48 HPLC:TChl-a:Bottle mg/m^3 0.413 0.413 49 Flag:HPLC:Bottle n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 7 F ' ' 2 9 -99 ' ' 6 F ' ' 1 10 -99 ' ' 8 F ' ' 3 11 -99 ' ' 7 F ' ' 1 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2017-001 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P13 EVENT NUMBER : 74 LATITUDE : 49 2.49000 N ! (deg min) LONGITUDE : 131 39.96000 W ! (deg min) WATER DEPTH : 3014 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer standardized with IAPSO standard seawater 7 - 25 days after collection. For details on duplicate analysis see document 2017-001_SAL.pdf. Oxygen samples were analyzed at sea and on shore using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see document 2017-001_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2017-001_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS within ~2 weeks of the cruise. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.01 to 1.69 µg/l. Average CV for this cruise was 3.72% with 7 out of 104 duplicate pairs having a CV > 10% and 1 out of 104 duplicate pairs having a CV > 30%. Our average dataset CV is 3.81% for 2013 - 2016 so the overall quality of this dataset is very good. For details on duplicates see file 2017-001_CHL.pdf. DMS samples were collected in 250 ml ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100 ml/min. The DMS was extracted from the water and absorbed on to a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a Dewar containing boiling water) onto a Chromasorb 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as <0.1 nmol/l. For more details see file 2017-001_DMS_report.pdf and for duplicate analysis see 2017-001_DMS.pdf. DMSP-D: 20-75 ml of seawater were allowed to flow directly from the Niskin into a magnetic filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For details on analysis see document 2017-001_DMS_report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis of unfiltered samples. Total alkalinity (ALK) was determined by potentiometric titration. For duplicates and precision of DIC and ALK samples see files 2017-001_DIC.pdf and 2017-001_ALK.pdf respectively. Dissolved Organic Carbon (DOC), Total Organic Carbon (TOC), Total Dissolved Nitrogen (TDN), and Total Nitrogen (TN) samples were collected in 80 ml polycarbonate bottles and frozen until analysis. DOC/TDN samples were filtered inline from the Niskin bottles, using acid-washed filter holders and tubing and pre-combusted GF/F filters. For analysis, samples were thawed and acidified with 4M HCl at a ratio of 100 µl HCL to 40 ml seawater. DOC/TDN samples were collected for depths < 250 meters, and TOC/TN samples were collected for depths > 250 meters. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~1 month after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. The transmissivity channel was removed because of frequent shifts in values during casts and large variations between values during repeat casts. The data are available, by request, from the chief scientist. Oxygen:Dissolved:CTD was calibrated using the method described in SeaBird Application Note #64-2, June 2012 revision, except that a small offset in the fit was allowed. NOTE: While the CTD fluorescence data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2017-001-proc.pdf $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- -2-- 3- --4--- ---5---- ---6---- ---7---- --8--- --9-- ---10-- --11-- ---12--- 13 --14-- 15 ---16-- 17 --18-- 19 --20-- 21 --22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- 49 !Samp Bott Bo Pressu Temperat Salinity Sigma-t: Oxygen Oxyge Fluores PAR: Salinity Fl Nitrat Fl Phospha Fl Silica Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ tt re:CTD ure:CTD :CTD CTD :Disso n:Dis cence: CTD :Bottle ag e_plus ag te: ag te: ag phyll: ag Pigmen Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: C2mgdg: DVChl- Chl-a: Phe: Car: TChl-a: ag !Numb Numb ~u lved: solve CTD: ~o _Nitri ~o Bottle ~o Bottle ~o Extrac ~o t:Extr Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle ~o !er er en CTD: d:CTD Seapoin tt te: tt tt tt ted: tt acted: Bottle Bottle Bottle Bottle Bottle Bottle tt ! ce Volume :Mass t le Bottle le le le Bottle le Bottle le !---- ---- -- ------ -------- -------- -------- ------ ----- ------- ------ -------- -- ------ -- ------- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 428 1 1 5.7 7.6413 32.6082 25.4729 6.66 290.0 0.483 176.3 32.6119 0 7.90 0 0.861 0 9.06 0 0.40 6 0.15 0.059 0.000 0.003 0.044 0.000 0.000 0.000 0.000 0.045 0.035 0.002 0.003 0.000 0.161 0.024 0.016 0.012 0.007 0.004 0.051 0.014 0.000 0.413 0.000 0.004 0.413 6