*2023/01/31 08:53:14.73 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2016/08/25 20:48:45.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 24 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 2DCD735C NUMBER OF CHANNELS : 12 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 658 681 2 Bottle_Number n/a 1 24 3 Bottle:Firing_Sequence n/a 1 24 4 Pressure:CTD dbar 4.9 149.2 5 Temperature:CTD deg_C_(ITS90) 5.345 16.3939 6 Salinity:CTD PSS-78 32.5221 33.7679 7 Sigma-t:CTD kg/m^3 23.7802 26.6843 8 Transmissivity:CTD %/m 50.7 60 9 Oxygen:Dissolved:CTD:Volume ml/l 4.03 7.02 10 Oxygen:Dissolved:CTD:Mass µmol/kg 175.2 305.7 11 Fluorescence:CTD:Seapoint mg/m^3 0.109 1.468 12 PAR:CTD µE/m^2/sec 0.1 387.5 $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 7 F ' ' 1 $END *ADMINISTRATION MISSION : 2016-008 AGENCY : IOS, Ocean Sciences Division, Sidney COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P26 EVENT NUMBER : 84 LATITUDE : 50 0.09000 N ! (deg min) LONGITUDE : 144 59.87000 W ! (deg min) WATER DEPTH : 4251 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8410A Portasal Salinometer (S/N 58879) standardized with IAPSO standard seawater within 50 - 64 days after collection. Data are reported to the third decimal place on account of the reduced accuracy and precision from the delay in analysis and the slightly less accurate instrument (±0.001 PSU less accurate the the 8400B Autosal). For details on duplicate analysis see file 2016-008_SAL.pdf. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2016-008_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2016-008_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS within ~4 weeks of the cruise. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.02 to 43.38 µg/l. Average CV for this cruise was 3.66% with 2 out of 63 duplicate pairs having a CV > 15% and 0 out of 63 duplicate pairs having a CV > 50%. Our average dataset CV is 2.83% for 2013 - 2015 so the overall quality of this dataset is very good. For details on duplicates see file 2016-008_CHL.pdf. DMS samples were collected in 250 ml ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100 ml/min. The DMS was extracted from the water and absorbed on to a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a Dewar containing boiling water) onto a Chromasorb 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as <0.1 nmol/l. For more details see file 2016-008_DMS_report.pdf and for duplicate analysis see 2016-008_DMS.pdf. DMSP-D: Approximately 20-75 ml of seawater were allowed to flow directly from the Niskin into a filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For details on analysis see document 2016-008_DMS_report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogren ion concentration scale was determined photometrically and is reported at 25C and surface pressure. For duplicates and precision of DIC, ALK and pH samples see files 2016-008_DIC.pdf, 2016-008_ALK.pdf, and 2016-008_pH.pdf respectively. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 weeks after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. Oxygen:Dissolved:CTD was calibrated using the method described in Sea-Bird Application Note #64-2 of June 2012 (Sea-Bird_64-2_Jun2012.pdf), except that a small offset in the fit was allowed. NOTE: While the Fluorescence:CTD data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2016-008-proc.pdf. $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- -2-- -3-- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- --12-- !Samp Bott Bott Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores PAR: !le_ le_ le:F re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: CTD !Numb Numb ~ng_ vity: lved: solve CTD: !er er Sequ CTD CTD: d:CTD Seapoin ! ence Volume :Mass t !---- ---- ---- ------ -------- -------- -------- ----- ------ ----- ------- ------ *END OF HEADER 681 24 24 4.9 16.3939 32.5227 23.7802 50.9 5.96 260.1 0.427 386.5 680 23 23 4.9 16.3839 32.5227 23.7825 50.9 5.99 261.0 0.491 387.5 679 22 22 5.0 16.3827 32.5226 23.7827 50.8 5.98 260.6 0.446 379.5 678 21 21 4.9 16.3796 32.5224 23.7832 50.9 5.97 260.5 0.470 381.8 677 20 20 4.9 16.3779 32.5222 23.7834 50.7 5.97 260.4 0.459 384.0 676 19 19 5.0 16.3813 32.5221 23.7826 51.0 5.98 260.8 0.446 382.5 675 18 18 50.7 7.2828 32.7898 25.6655 55.5 7.02 305.7 1.468 18.4 674 17 17 50.9 7.2800 32.7898 25.6659 55.5 7.02 305.7 1.463 18.9 673 16 16 50.7 7.2802 32.7898 25.6659 55.4 7.01 305.3 1.451 19.9 672 15 15 50.8 7.2809 32.7898 25.6658 55.4 7.01 305.3 1.456 20.9 671 14 14 50.8 7.2836 32.7897 25.6653 55.4 7.02 305.6 1.460 22.0 670 13 13 50.6 7.2845 32.7896 25.6651 55.5 7.01 305.3 1.444 22.2 669 12 12 99.8 6.1386 33.2204 26.1551 59.7 5.84 254.2 0.238 1.3 668 11 11 99.9 6.1375 33.2216 26.1562 59.7 5.83 253.7 0.240 1.3 667 10 10 99.8 6.1383 33.2210 26.1556 59.7 5.83 253.8 0.237 1.4 666 9 9 99.8 6.1391 33.2202 26.1549 59.6 5.83 253.7 0.236 1.4 665 8 8 99.7 6.1362 33.2222 26.1568 59.7 5.82 253.2 0.230 1.4 664 7 7 100.2 6.1347 33.2237 26.1582 59.6 5.82 253.1 0.237 1.4 663 6 6 149.0 5.3450 33.7678 26.6843 60.0 4.03 175.2 0.111 0.1 662 5 5 149.0 5.3456 33.7677 26.6842 60.0 4.04 175.6 0.109 0.1 661 4 4 149.1 5.3456 33.7676 26.6841 60.0 4.03 175.4 0.113 0.1 660 3 3 149.2 5.3452 33.7677 26.6842 60.0 4.04 175.5 0.116 0.1 659 2 2 148.8 5.3450 33.7677 26.6842 59.9 4.03 175.2 0.109 0.1 658 1 1 149.1 5.3456 33.7679 26.6843 60.0 4.04 175.5 0.113 0.1