*2023/01/31 08:53:14.32 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2016/08/23 04:44:49.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 1 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 4AAB65FC NUMBER OF CHANNELS : 50 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 493 493 2 Bottle_Number n/a 1 1 3 Bottle:Firing_Sequence n/a 1 1 4 Pressure:CTD dbar 5.2 5.2 5 Temperature:CTD deg_C_(ITS90) 16.6363 16.6363 6 Salinity:CTD PSS-78 32.4907 32.4907 7 Sigma-t:CTD kg/m^3 23.7001 23.7001 8 Transmissivity:CTD %/m 51.1 51.1 9 Oxygen:Dissolved:CTD:Volume ml/l 5.87 5.87 10 Oxygen:Dissolved:CTD:Mass µmol/kg 255.8 255.8 11 Fluorescence:CTD:Seapoint mg/m^3 1.327 1.327 12 PAR:CTD µE/m^2/sec 0 0 13 Salinity:Bottle PSS-78 32.495 32.495 14 Flag:Salinity:Bottle n/a 15 Nitrate_plus_Nitrite:Bottle µmol/l 0.13 0.13 16 Flag:Nitrate_plus_Nitrite:Bottle n/a 17 Phosphate:Bottle µmol/l 0.42 0.42 18 Flag:Phosphate:Bottle n/a 19 Silicate:Bottle µmol/l 3.56 3.56 20 Flag:Silicate:Bottle n/a 21 Chlorophyll:Extracted:Bottle mg/m^3 0.26 0.26 22 Flag:Chlorophyll:Extracted:Bottle n/a 23 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.4E-01 0.4E-01 24 HPLC:Chl-c3:Bottle mg/m^3 0.27E-01 0.27E-01 25 HPLC:Chlide-a:Bottle mg/m^3 0 0 26 HPLC:MgDVP:Bottle mg/m^3 0 0 27 HPLC:Chl-c2:Bottle mg/m^3 0.28E-01 0.28E-01 28 HPLC:Chl-c1:Bottle mg/m^3 0 0 29 HPLC:Me-chlide:Bottle mg/m^3 0 0 30 HPLC:Peri:Bottle mg/m^3 0 0 31 HPLC:Pheide-a:Bottle mg/m^3 0 0 32 HPLC:But-fuco:Bottle mg/m^3 0.26E-01 0.26E-01 33 HPLC:Fuco:Bottle mg/m^3 0.3E-01 0.3E-01 34 HPLC:Neo:Bottle mg/m^3 0 0 35 HPLC:Pras:Bottle mg/m^3 0 0 36 HPLC:Viola:Bottle mg/m^3 0 0 37 HPLC:Hex-fuco:Bottle mg/m^3 0.128 0.128 38 HPLC:Diadino:Bottle mg/m^3 0.47E-01 0.47E-01 39 HPLC:Allo:Bottle mg/m^3 0 0 40 HPLC:Diato:Bottle mg/m^3 0.3E-02 0.3E-02 41 HPLC:Zea:Bottle mg/m^3 0.7E-02 0.7E-02 42 HPLC:Lut:Bottle mg/m^3 0 0 43 HPLC:Chl-b:Bottle mg/m^3 0.2E-01 0.2E-01 44 HPLC:C2mgdg:Bottle mg/m^3 0 0 45 HPLC:DVChl-a:Bottle mg/m^3 0 0 46 HPLC:Chl-a:Bottle mg/m^3 0.244 0.244 47 HPLC:Phe:Bottle mg/m^3 0 0 48 HPLC:B-Car:Bottle mg/m^3 0 0 49 HPLC:TChl-a:Bottle mg/m^3 0.244 0.244 50 Flag:HPLC:Bottle n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 7 F ' ' 1 13 -99 ' ' 8 F R4 3 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 7 F R4 2 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 8 F R4 3 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2016-008 AGENCY : IOS, Ocean Sciences Division, Sidney COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P18 EVENT NUMBER : 59 LATITUDE : 49 25.95000 N ! (deg min) LONGITUDE : 136 39.76000 W ! (deg min) WATER DEPTH : 3835 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8410A Portasal Salinometer (S/N 58879) standardized with IAPSO standard seawater within 50 - 64 days after collection. Data are reported to the third decimal place on account of the reduced accuracy and precision from the delay in analysis and the slightly less accurate instrument (±0.001 PSU less accurate the the 8400B Autosal). For details on duplicate analysis see file 2016-008_SAL.pdf. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2016-008_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2016-008_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS within ~4 weeks of the cruise. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). Flags and comments apply to chlorophyll values only. Precision Statement: Chlorophyll values ranged from 0.02 to 43.38 µg/l. Average CV for this cruise was 3.66% with 2 out of 63 duplicate pairs having a CV > 15% and 0 out of 63 duplicate pairs having a CV > 50%. Our average dataset CV is 2.83% for 2013 - 2015 so the overall quality of this dataset is very good. For details on duplicates see file 2016-008_CHL.pdf. DMS samples were collected in 250 ml ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100 ml/min. The DMS was extracted from the water and absorbed on to a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a Dewar containing boiling water) onto a Chromasorb 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as <0.1 nmol/l. For more details see file 2016-008_DMS_report.pdf and for duplicate analysis see 2016-008_DMS.pdf. DMSP-D: Approximately 20-75 ml of seawater were allowed to flow directly from the Niskin into a filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For details on analysis see document 2016-008_DMS_report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogren ion concentration scale was determined photometrically and is reported at 25C and surface pressure. For duplicates and precision of DIC, ALK and pH samples see files 2016-008_DIC.pdf, 2016-008_ALK.pdf, and 2016-008_pH.pdf respectively. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~2 weeks after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. Oxygen:Dissolved:CTD was calibrated using the method described in Sea-Bird Application Note #64-2 of June 2012 (Sea-Bird_64-2_Jun2012.pdf), except that a small offset in the fit was allowed. NOTE: While the Fluorescence:CTD data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2016-008-proc.pdf. $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- -2-- -3-- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- --12-- ---13-- 14 --15-- 16 ---17-- 18 --19-- 20 --21-- 22 --23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- 50 !Samp Bott Bott Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores PAR: Salinit Fl Nitrat Fl Phospha Fl Silica Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ le_ le:F re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: CTD y: ag e_plus ag te: ag te: ag phyll: ag Pigmen Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: C2mgdg: DVChl- Chl-a: Phe: Car: TChl-a: ag !Numb Numb ~ng_ vity: lved: solve CTD: Bottle ~o _Nitri ~o Bottle ~o Bottle ~o Extrac ~o t:Extr Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle ~o !er er Sequ CTD CTD: d:CTD Seapoin tt te: tt tt tt ted: tt acted: Bottle Bottle Bottle Bottle Bottle Bottle tt ! ence Volume :Mass t le Bottle le le le Bottle le Bottle le !---- ---- ---- ------ -------- -------- -------- ----- ------ ----- ------- ------ ------- -- ------ -- ------- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 493 1 1 5.2 16.6363 32.4907 23.7001 51.1 5.87 255.8 1.327 0.0 32.495 0 0.13 0 0.420 0 3.56 0 0.26 6 0.04 0.027 0.000 0.000 0.028 0.000 0.000 0.000 0.000 0.026 0.030 0.000 0.000 0.000 0.128 0.047 0.000 0.003 0.007 0.000 0.020 0.000 0.000 0.244 0.000 0.000 0.244 6