*2023/01/12 08:13:46.78 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2016/06/14 21:57:48.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 21 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 9E7C3ABA NUMBER OF CHANNELS : 11 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 518 538 2 Bottle_Number n/a 1 22 3 Bottle:Firing_Sequence n/a 1 21 4 Pressure:CTD dbar 9.3 4003.1 5 Temperature:CTD deg_C_(ITS90) 1.504 9.311 6 Salinity:CTD PSS-78 32.6779 34.6809 7 Sigma-t:CTD kg/m^3 25.2772 27.7795 8 Transmissivity:CTD %/m 46.4 62.3 9 Oxygen:Dissolved:CTD:Volume ml/l 0.49 6.93 10 Oxygen:Dissolved:CTD:Mass µmol/kg 21.4 301.8 11 Fluorescence:CTD:Seapoint mg/m^3 0.61E-01 2.225 $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 $END *ADMINISTRATION MISSION : 2016-006 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P26 EVENT NUMBER : 93 LATITUDE : 50 0.02000 N ! (deg min) LONGITUDE : 144 59.99000 W ! (deg min) WATER DEPTH : 4251 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 9 to 28 days after collection. NOTE: The pooled standard deviation (0.002) is poorer than expected given the very reasonable length of time from sample collection to analysis. Values are reported to the third decimal place as a result. For details on duplicate analysis see file 2016-006_SAL.pdf. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2016-006_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2016-006_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS ~4 weeks afer the cruise. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). Precision Statement: Chlorophyll values ranged from 0.04-12.53 µg/l. Average CV for this cruise was 3.12% with 0 out of 59 duplicate pairs having a CV > 15% or a CV > 50%. Our average dataset CV is 2.83% for 2013 - 2015 so the overall quality of this dataset is excellent. Flags and comments apply to chlorophyll values only. For details on duplicates see file 2016-006_CHL.pdf. DMS samples were collected in 250 ml ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100 ml/min. The DMS was extracted from the water and absorbed on to a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100 deg C (with a Dewar containing boiling water) onto a Chromasorb 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as < 0.1 nmol/l. For more details on analysis see file 2016-006_DMS_report.pdf and for duplicate analysis see 2016-006_DMS.pdf. DMSP-D: Approximately 20-75 ml of seawater were allowed to flow directly from the Niskin into a filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution (50%). DMSP-D and DMSP-T: Samples were stored in the dark at 4 deg C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For more details see file 2016-006_DMS_report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis of unfiltered samples. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogen ion concentration scale was determined photometrically and is reported at 25C and surface pressure. For duplicates and precision of DIC, ALK and pH samples see files 2016-006_DIC.pdf, 2016-006_ALK.pdf, and 2016-006_pH.pdf respectively. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~4-5 weeks after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. Oxygen:Dissolved:CTD was calibrated using the method described in SeaBird Application Note #64-2, June 2012 revision, except that a small offset in the fit was allowed. NOTE: While the Fluorescence:CTD data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on the processing see the report: 2016-006-proc.pdf. $REMARKS Quality flags have the following significance: ----------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: !Numb ~u ~u vity: lved: solve CTD: !er mb en CTD CTD: d:CTD Seapoin ! er ce Volume :Mass t !---- -- -- ------ -------- -------- -------- ----- ------ ----- ------- *END OF HEADER 538 22 5 1999.6 1.9546 34.5814 27.6656 62.2 1.24 54.0 0.061 537 21 4 2498.9 1.7261 34.6269 27.7197 62.3 1.90 82.6 0.061 536 20 3 2998.2 1.5852 34.6551 27.7528 62.3 2.48 107.6 0.061 535 19 2 3497.6 1.5158 34.6718 27.7714 61.8 2.88 125.2 0.061 534 18 1 4003.1 1.5040 34.6809 27.7795 62.2 3.11 135.3 0.061 533 16 21 9.3 9.3110 32.6781 25.2774 46.7 6.93 301.6 2.153 532 15 20 9.5 9.3099 32.6779 25.2774 47.2 6.93 301.8 2.146 531 14 19 9.8 9.3109 32.6779 25.2772 46.5 6.91 300.9 2.225 530 13 18 9.4 9.3105 32.6779 25.2773 46.4 6.91 300.8 2.132 529 12 17 498.0 3.8504 34.0957 27.1078 61.8 0.94 40.8 0.073 528 11 16 499.7 3.8497 34.0958 27.1079 61.8 0.94 40.8 0.073 527 10 15 498.2 3.8494 34.0958 27.1079 61.6 0.94 40.8 0.073 526 9 14 497.3 3.8497 34.0956 27.1077 61.7 0.94 40.9 0.073 525 8 13 998.8 2.9202 34.3767 27.4205 61.8 0.49 21.5 0.062 524 7 12 999.5 2.9198 34.3768 27.4206 61.9 0.50 21.6 0.063 523 6 11 998.6 2.9198 34.3767 27.4205 61.8 0.49 21.4 0.062 522 5 10 997.9 2.9203 34.3765 27.4203 61.8 0.49 21.5 0.062 521 4 9 1999.3 1.9544 34.5814 27.6656 62.2 1.24 53.8 0.061 520 3 8 1998.9 1.9544 34.5813 27.6655 62.2 1.25 54.1 0.061 519 2 7 1999.3 1.9544 34.5813 27.6655 62.3 1.24 53.9 0.061 518 1 6 1999.9 1.9545 34.5814 27.6656 62.2 1.24 53.8 0.061