*2022/11/21 14:38:46.12 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2016/02/13 07:59:46.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 24 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 29529DCE NUMBER OF CHANNELS : 11 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 364 387 2 Bottle_Number n/a 1 24 3 Bottle:Firing_Sequence n/a 1 24 4 Pressure:CTD dbar 200.4 501.2 5 Temperature:CTD deg_C_(ITS90) 4.0898 6.2633 6 Salinity:CTD PSS-78 33.829 34.0528 7 Sigma-t:CTD kg/m^3 26.6198 27.0492 8 Transmissivity:CTD %/m 61 61.8 9 Oxygen:Dissolved:CTD:Volume ml/l 0.98 3.67 10 Oxygen:Dissolved:CTD:Mass µmol/kg 42.7 159.6 11 Fluorescence:CTD:Seapoint mg/m^3 0.49E-01 0.66E-01 $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 5 I I 0 3 -99 ' ' 5 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 $END *ADMINISTRATION MISSION : 2016-001 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P16 EVENT NUMBER : 49 LATITUDE : 49 17.02000 N ! (deg min) LONGITUDE : 134 39.95000 W ! (deg min) WATER DEPTH : 3609 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0585 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 12 to 21 days after collection. For details on duplicate analysis see file 2016-001_SAL.pdf. Oxygen samples were analyzed at sea using an automated Winkler titration system (Metrohm Dosimat model 876 and a UV light source and detector with a 365nm filter controlled by LV02_876 software designed and constructed by Scripps Institution of Oceanography) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2016-001_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2016-001_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated annually with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS ~7 weeks after the cruise. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). Precision Statement: Chlorophyll values ranged from 0.04-1.14 mg/m^3. Average CV for this cruise was an excellent 2.82% with 1 out of 58 duplicate pairs having a CV > 15% and 0 out of 58 duplicate pairs having a CV > 50% . Our average dataset CV is 2.83% for 2013 - 2015 so the overall quality of this dataset is excellent. For details on duplicates see file 2016-001_CHL.pdf. Flags and comments apply to chlorophyll values only. No flags or comments are assigned for Phaeopigment values. DMS samples were collected in 250 ml ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100 ml/min. The DMS was extracted from the water and absorbed on to a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100C (with a Dewar containing boiling water) onto a Chromasorb 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as < 0.1 nmol/l. For more details see file 2016-001_DMS_report.pdf and for duplicate analysis see 2016-001_DMS.pdf. DMSP-D: Approximately 20-75 ml of seawater were allowed to flow directly from the Niskin into a filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nmol/l, so “0” values should be interpreted as < 0.1 nmol/l. DMSP data are limited to 2 significant figures. For details on analysis see document 2016-001_DMS_report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis of unfiltered samples. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogen ion concentration scale was determined photometrically and is reported at 25C and surface pressure. For duplicates and precision of DIC, ALK and pH samples see files 2016-001_DIC.pdf, 2016-001_ALK.pdf, and 2016-001_pH.pdf respectively. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~3 months after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. NOTE: While the Fluorescence:CTD data are expressed in concentration units, they do not always compare well to extracted chlorophyll samples, particularly for casts far from shore. It is recommended that users check extracted chlorophyll values where available. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. NOTE: The transmissivity data were removed for files 2016-001-0002 and 2016-001-0005 because of poor data due to a cable problem. Oxygen:Dissolved:CTD was calibrated using the method described in the Sea-Bird Application Note #64-2 of June 2012 (Sea-Bird_64-2_Jun2012.pdf), except that a small offset in the fit was allowed. For details on the processing see the report: 2016-001-proc.pdf. $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- -2-- -3-- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- !Samp Bott Bott Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores !le_ le_ le:F re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: !Numb Numb ~ng_ vity: lved: solve CTD: !er er Sequ CTD CTD: d:CTD Seapoin ! ence Volume :Mass t !---- ---- ---- ------ -------- -------- -------- ----- ------ ----- ------- *END OF HEADER 387 24 24 201.6 6.2597 33.8294 26.6206 61.0 3.66 159.2 0.050 386 23 23 201.2 6.2587 33.8294 26.6207 61.0 3.67 159.5 0.053 385 22 22 202.2 6.2575 33.8295 26.6209 61.0 3.66 159.0 0.052 384 21 21 201.5 6.2572 33.8295 26.6210 61.0 3.67 159.6 0.066 383 20 20 200.4 6.2631 33.8291 26.6199 61.0 3.66 159.3 0.059 382 19 19 200.7 6.2633 33.8290 26.6198 61.0 3.67 159.5 0.050 381 18 18 300.0 4.9505 33.8433 26.7896 61.6 2.62 113.9 0.050 380 17 17 301.2 4.9506 33.8437 26.7899 61.5 2.62 113.7 0.051 379 16 16 302.5 4.9516 33.8437 26.7898 61.5 2.60 113.0 0.050 378 15 15 302.8 4.9511 33.8438 26.7900 61.6 2.62 113.8 0.050 377 14 14 301.8 4.9514 33.8441 26.7902 61.6 2.63 114.2 0.049 376 13 13 299.6 4.9503 33.8434 26.7897 61.6 2.62 114.1 0.049 375 12 12 402.0 4.3017 33.9202 26.9216 61.7 1.66 72.1 0.050 374 11 11 403.4 4.3015 33.9202 26.9217 61.7 1.66 72.2 0.050 373 10 10 403.5 4.3012 33.9203 26.9218 61.7 1.66 72.2 0.050 372 9 9 402.2 4.3016 33.9204 26.9218 61.7 1.66 72.2 0.050 371 8 8 400.8 4.3015 33.9204 26.9218 61.7 1.66 72.2 0.050 370 7 7 400.8 4.3013 33.9203 26.9218 61.7 1.66 72.1 0.050 369 6 6 501.0 4.0900 34.0528 27.0492 61.8 0.98 42.7 0.050 368 5 5 501.2 4.0898 34.0528 27.0492 61.7 0.98 42.8 0.050 367 4 4 501.1 4.0912 34.0525 27.0488 61.8 0.98 42.8 0.051 366 3 3 501.1 4.0907 34.0527 27.0490 61.7 0.98 42.8 0.051 365 2 2 501.0 4.0913 34.0526 27.0489 61.7 0.99 42.9 0.050 364 1 1 500.8 4.0921 34.0524 27.0486 61.8 0.98 42.7 0.050