*2022/11/03 11:10:28.45 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2015/02/15 05:37:27.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 14 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 4DDD095A NUMBER OF CHANNELS : 52 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- -------------------------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 470 483 2 Bottle_Number n/a 1 14 3 Bottle:Firing_Sequence n/a 1 14 4 Pressure:CTD dbar 3.8 301.7 5 Temperature:CTD deg_C_(ITS90) 5.0853 9.4377 6 Salinity:CTD PSS-78 32.1758 33.8718 7 Sigma-t:CTD kg/m^3 24.8647 26.7969 8 Transmissivity:CTD %/m 58.9 66.7 9 Oxygen:Dissolved:CTD:Volume ml/l 2.5 6.54 10 Oxygen:Dissolved:CTD:Mass µmol/kg 108.6 285.1 11 Fluorescence:CTD:Seapoint mg/m^3 0.59E-01 1.158 12 Nitrate_plus_Nitrite:Bottle µmol/l 5.51 36.87 13 Flag:Nitrate_plus_Nitrite:Bottle n/a 14 Phosphate:Bottle µmol/l 0.736 2.545 15 Flag:Phosphate:Bottle n/a 16 Silicate:Bottle µmol/l 6.9 64.85 17 Flag:Silicate:Bottle n/a 18 Chlorophyll:Extracted:Bottle mg/m^3 0.3E-01 0.5 19 Flag:Chlorophyll:Extracted:Bottle n/a 20 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.4E-01 0.18 21 Dimethyl_Sulphide:Bottle nmol/l 0 4.58 22 Flag:Dimethyl_Sulphide:Bottle n/a 23 Dimethylsulfoniopropionate_Dissolved:Bottle nmol/l -99 -99 24 Flag:Dimethylsulfoniopropionate_Dissolved:Bottle n/a 25 Dimethylsulfoniopropionate_Total:Bottle nmol/l -99 -99 26 Flag:Dimethylsulfoniopropionate_Total:Bottle n/a 27 HPLC:Chl-c3:Bottle mg/m^3 0.55E-01 0.67E-01 28 HPLC:Chlide-a:Bottle mg/m^3 0 0 29 HPLC:MgDVP:Bottle mg/m^3 0.6E-02 0.8E-02 30 HPLC:Chl-c2:Bottle mg/m^3 0.44E-01 0.49E-01 31 HPLC:Chl-c1:Bottle mg/m^3 0 0 32 HPLC:Me-chlide:Bottle mg/m^3 0 0 33 HPLC:Peri:Bottle mg/m^3 0 0.12E-01 34 HPLC:Pheide-a:Bottle mg/m^3 0 0 35 HPLC:But-fuco:Bottle mg/m^3 0.52E-01 0.59E-01 36 HPLC:Fuco:Bottle mg/m^3 0.31E-01 0.35E-01 37 HPLC:Neo:Bottle mg/m^3 0.1E-01 0.11E-01 38 HPLC:Pras:Bottle mg/m^3 0.11E-01 0.12E-01 39 HPLC:Viola:Bottle mg/m^3 0.4E-02 0.6E-02 40 HPLC:Hex-fuco:Bottle mg/m^3 0.212 0.216 41 HPLC:Diadino:Bottle mg/m^3 0.28E-01 0.37E-01 42 HPLC:Allo:Bottle mg/m^3 0.5E-02 0.8E-02 43 HPLC:Diato:Bottle mg/m^3 0 0.2E-02 44 HPLC:Zea:Bottle mg/m^3 0.15E-01 0.17E-01 45 HPLC:Lut:Bottle mg/m^3 0.4E-02 0.7E-02 46 HPLC:Chl-b:Bottle mg/m^3 0.73E-01 0.82E-01 47 HPLC:DVChl-a:Bottle mg/m^3 0.2E-02 0.7E-02 48 HPLC:Chl-a:Bottle mg/m^3 0.413 0.434 49 HPLC:Phe-a:Bottle mg/m^3 0 0 50 HPLC:B-Car:Bottle mg/m^3 0 0.6E-02 51 HPLC:TChl-a:Bottle mg/m^3 0.416 0.441 52 Flag:HPLC:Bottle n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 7 F R4 2 13 0 ' ' 3 NQ C ' ' 14 -99 ' ' 8 F R4 3 15 0 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 0 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 0 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 7 F R4 2 22 0 ' ' 3 NQ C ' ' 23 -99 ' ' 7 F R4 2 24 0 ' ' 3 NQ C ' ' 25 -99 ' ' 7 F R4 2 26 0 ' ' 3 NQ C ' ' 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 8 F R4 3 49 -99 ' ' 8 F R4 3 50 -99 ' ' 8 F R4 3 51 -99 ' ' 8 F R4 3 52 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2015-001 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P16 EVENT NUMBER : 53 LATITUDE : 49 17.01000 N ! (deg min) LONGITUDE : 134 40.01000 W ! (deg min) WATER DEPTH : 3633 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0506 LOCATION : Mid-ship ! custom item *COMMENTS Samples Number 471, 472, 473, 479, 482, 483: DMSP-D:Bottle and DMSP-T:Bottle - elevated levels in the blank therefore sample data not valid. Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable nylon inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 10 to 22 days after collection. For details on duplicate analysis see file 2015-001_SAL.pdf. Oxygen samples were analyzed at sea using an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2015-001_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2015-001_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated annually with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS ~4 weeks after the cruise. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). For details on duplicates see file 2015-001_CHL.pdf. Flags and comments apply to chlorophyll values only. No flags or comments are assigned for Phaeopigment values. DMS samples were collected in 250 ml ground glass stoppered bottles and stored in a fridge, in the dark and removed one at a time before analysis. A sample was loaded onto the stripper and purged with UHP Nitrogen for 10 minutes at ~100 ml/min. The DMS was extracted from the water and absorbed on to a Tenax TA trap kept at -80C. The trap was subsequently desorbed at 100C (with a Dewar containing boiling water) onto a Chromasorb 330 column which eluted onto a Flame Photometric Detector (FPD). All samples were run as soon as possible after being collected. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as <0.10 nmol/l. For more details see file 2015-001_DMS_report.pdf and for duplicate analysis see 2015-001_DMS.pdf. DMSP-D: Approximately 20-75 ml of seawater were allowed to flow directly from the Niskin into a filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube (15 ml) containing 50 µl of a 50% sulphuric acid solution (50%). DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level for DMS is 0.1 nmol/l, so “0” values should be interpreted as <0.1 nmol/l. For more details see file 2015-001_DMS_report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis of unfiltered samples. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogen ion concentration scale was determined photometrically and is reported at 25C and surface pressure. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~1 month after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. Oxygen:Dissolved:CTD was calibrated using the method described in the Sea-Bird Application Note #64-2 of June 2012 (Sea-Bird_64-2_Jun2012.pdf), except that a small offset in the fit was allowed. For details on the processing see the report: 2015-001-proc.pdf. $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- --12-- 13 ---14-- 15 --16-- 17 --18-- 19 --20-- --21-- 22 --23-- 24 --25-- 26 ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- ---48-- ---49-- ---50-- ---51-- 52 !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores Nitrat Fl Phospha Fl Silica Fl Chloro Fl Phaeo- Dimeth Fl Dimeth Fl Dimeth Fl HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: e_plus ag te: ag te: ag phyll: ag Pigmen yl_ ag ylsulf ag ylsulf ag Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: DVChl- Chl-a: Phe-a: Car: TChl-a: ag !Numb ~u ~u vity: lved: solve CTD: _Nitri ~o Bottle ~o Bottle ~o Extrac ~o t:Extr Sulphi ~o ~_Diss ~o ~nate_ ~o Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle ~o !er mb en CTD CTD: d:CTD Seapoin te: tt tt tt ted: tt acted: de: tt olved: tt Total: tt Bottle Bottle Bottle Bottle Bottle Bottle tt ! er ce Volume :Mass t Bottle le le le Bottle le Bottle Bottle le Bottle le Bottle le le !---- -- -- ------ -------- -------- -------- ----- ------ ----- ------- ------ -- ------- -- ------ -- ------ -- ------ ------ -- ------ -- ------ -- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 483 14 14 3.8 9.4372 32.1758 24.8647 58.9 6.54 285.1 1.118 5.60 0 0.745 0 7.07 0 0.50 0 0.16 4.58 0 -99.00 1 -99.00 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 482 13 13 5.2 9.4377 32.2390 24.9140 59.1 6.54 284.8 1.106 5.52 0 0.740 0 7.00 0 0.48 6 0.16 3.96 0 -99.00 1 -99.00 1 0.067 0.000 0.006 0.044 0.000 0.000 0.000 0.000 0.052 0.031 0.011 0.012 0.006 0.214 0.037 0.008 0.002 0.017 0.007 0.073 0.003 0.413 0.000 0.006 0.416 6 481 12 12 11.5 9.4331 32.2395 24.9151 59.3 6.53 284.6 1.116 5.55 6 0.740 6 6.96 6 0.50 0 0.17 3.75 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 480 11 11 15.8 9.4291 32.2402 24.9163 59.3 6.52 284.1 1.158 5.51 0 0.743 0 6.93 0 0.49 0 0.17 3.76 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 479 10 10 21.6 9.4269 32.2402 24.9167 59.4 6.53 284.2 1.146 5.54 0 0.736 0 6.90 0 0.47 6 0.18 3.91 6 -99.00 1 -99.00 1 0.055 0.000 0.008 0.049 0.000 0.000 0.000 0.000 0.058 0.034 0.010 0.011 0.006 0.216 0.028 0.005 0.000 0.015 0.004 0.082 0.007 0.434 0.000 0.002 0.441 6 478 9 9 26.4 9.4218 32.2406 24.9178 59.5 6.52 284.0 1.149 5.54 0 0.739 0 6.90 0 0.50 0 0.18 3.81 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 477 8 8 30.8 9.4195 32.2405 24.9181 59.7 6.52 284.0 1.137 5.55 0 0.738 0 6.91 0 0.48 0 0.17 3.03 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 476 7 7 41.0 9.4294 32.2395 24.9157 59.6 6.52 284.1 1.114 5.54 0 0.738 0 6.90 0 0.49 0 0.15 4.07 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 475 6 6 51.7 9.4347 32.2391 24.9146 59.7 6.52 284.0 1.112 5.53 0 0.743 0 6.91 0 0.47 6 0.15 3.38 0 -99.00 -99.00 0.064 0.000 0.007 0.048 0.000 0.000 0.012 0.000 0.059 0.035 0.010 0.011 0.004 0.212 0.032 0.005 0.000 0.017 0.006 0.074 0.002 0.422 0.000 0.000 0.424 6 474 5 5 76.8 9.1409 32.3158 25.0209 63.3 6.36 276.9 0.534 6.89 0 0.820 0 8.15 0 0.25 0 0.12 2.39 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 473 4 4 100.8 7.1574 33.1263 25.9475 66.2 5.49 238.9 0.082 18.74 0 1.457 0 22.06 0 0.03 0 0.04 0.00 0 -99.00 1 -99.00 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 472 3 3 177.6 6.7127 33.7788 26.5214 66.2 3.68 160.0 0.059 28.46 0 2.000 0 40.74 0 -99.00 0 -99.00 0.00 0 -99.00 1 -99.00 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 471 2 2 203.0 6.2822 33.8221 26.6119 66.4 3.72 161.9 0.059 29.46 0 2.061 0 44.85 0 -99.00 0 -99.00 0.00 0 -99.00 1 -99.00 1 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 470 1 1 301.7 5.0853 33.8718 26.7969 66.7 2.50 108.6 0.060 36.87 0 2.545 0 64.85 0 -99.00 0 -99.00 0.00 0 -99.00 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000