*2022/11/10 15:03:33.88 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2014/08/26 07:41:16.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 2 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 8980307D NUMBER OF CHANNELS : 48 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 461 462 2 Bottle_Number n/a 1 2 3 Bottle:Firing_Sequence n/a 1 2 4 Pressure:CTD dbar 5.2 27.9 5 Temperature:CTD deg_C_(ITS90) 13.5981 16.8203 6 Salinity:CTD PSS-78 32.2338 32.3275 7 Sigma-t:CTD kg/m^3 23.4605 24.2307 8 Transmissivity:CTD %/m 51.2 60.6 9 Oxygen:Dissolved:CTD:Volume ml/l 5.75 6.44 10 Oxygen:Dissolved:CTD:Mass µmol/kg 250.9 280.8 11 Fluorescence:CTD:Seapoint mg/m^3 0.56 3.208 12 Salinity:Bottle PSS-78 32.2426 32.2426 13 Flag:Salinity:Bottle n/a 14 Nitrate_plus_Nitrite:Bottle µmol/l 0 0.37 15 Flag:Nitrate_plus_Nitrite:Bottle n/a 16 Phosphate:Bottle µmol/l 0.385 0.454 17 Flag:Phosphate:Bottle n/a 18 Silicate:Bottle µmol/l 0.57 1.69 19 Flag:Silicate:Bottle n/a 20 Chlorophyll:Extracted:Bottle mg/m^3 0.16 0.67 21 Flag:Chlorophyll:Extracted:Bottle n/a 22 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.3E-01 0.25 23 HPLC:Chl-c3:Bottle mg/m^3 0.13E-01 0.92E-01 24 HPLC:Chlide-a:Bottle mg/m^3 0 0 25 HPLC:MgDVP:Bottle mg/m^3 0 0.5E-02 26 HPLC:Chl-c2:Bottle mg/m^3 0.18E-01 0.1 27 HPLC:Chl-c1:Bottle mg/m^3 0 0 28 HPLC:Me-chlide:Bottle mg/m^3 0 0 29 HPLC:Peri:Bottle mg/m^3 0 0.19E-01 30 HPLC:Pheide-a:Bottle mg/m^3 0 0 31 HPLC:But-fuco:Bottle mg/m^3 0.2E-01 0.83E-01 32 HPLC:Fuco:Bottle mg/m^3 0.16E-01 0.137 33 HPLC:Neo:Bottle mg/m^3 0 0.7E-02 34 HPLC:Pras:Bottle mg/m^3 0 0.1E-01 35 HPLC:Viola:Bottle mg/m^3 0 0 36 HPLC:Hex-fuco:Bottle mg/m^3 0.67E-01 0.223 37 HPLC:Diadino:Bottle mg/m^3 0.16E-01 0.32E-01 38 HPLC:Allo:Bottle mg/m^3 0 0 39 HPLC:Diato:Bottle mg/m^3 0 0 40 HPLC:Zea:Bottle mg/m^3 0 0.19E-01 41 HPLC:Lut:Bottle mg/m^3 0 0 42 HPLC:Chl-b:Bottle mg/m^3 0.4E-02 0.107 43 HPLC:DVChl-a:Bottle mg/m^3 0 0 44 HPLC:Chl-a:Bottle mg/m^3 0.108 0.485 45 HPLC:Phe:Bottle mg/m^3 0 0 46 HPLC:B-Car:Bottle mg/m^3 0 0.9E-02 47 HPLC:TChl-a:Bottle mg/m^3 0.108 0.485 48 Flag:HPLC:Bottle n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ----- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99.9 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 8 F R4 3 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2014-019 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P19 EVENT NUMBER : 75 LATITUDE : 49 29.98000 N ! (deg min) LONGITUDE : 137 39.94000 W ! (deg min) WATER DEPTH : 3910 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Ltd. and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab at IOS on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 4 to 19 days after collection. For details on duplicate analysis see file 2014-019_SAL.pdf. Oxygen samples were analyzed at sea using an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2014-019_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For precision and duplicate analysis see file 2014-019_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer calibrated with commercially pure chlorophyll a standard (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship during the cruise or at IOS about 7 weeks later. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV (std dev / mean*100). For details on duplicates see file 2014-019_CHL.pdf. Flags and comments apply to chlorophyll values only. No flags or comments are assigned for Phaeopigment values. DMS samples were analyzed at sea. The minimum detectable level for DMS is 0.10 nmol/l, so “0” values should be interpreted as <0.10 nmol/l. For details on the collection and analysis of the DMS data see file 2014-019_DMS_report.pdf and for duplicate analysis see 2014-019_DMS.pdf. DMSP-D: 20-75 ml of seawater were allowed to flow directly from the Niskin into a magnetic filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMPS-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nmol/l, so “0” values should be interpreted as <0.1 nmol/l. For details on analysis see document 2014-019_DMSP.pdf. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~4-5 months after collection. The average of two samples is reported. Variability is assessed as the CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that a few bad fluorescence values were replaced with pad values. Post-cruise calibration reports indicate that the primary temperature and conductivity sensors had insignificant drifts. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. Oxygen:Dissolved:CTD was calibrated using the method described in the Sea-Bird Application Note #64-2 of June 2012 (Sea-Bird_64-2_Jun2012.pdf), except that a small offset in the fit was allowed. For details on the processing see the report: 2014-019-proc.pdf. $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- ---12--- 13 --14-- 15 ---16-- 17 --18-- 19 --20-- 21 --22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- 48 !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores Salinity Fl Nitrat Fl Phospha Fl Silica Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: :Bottle ag e_plus ag te: ag te: ag phyll: ag Pigmen Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: DVChl- Chl-a: Phe: Car: TChl-a: ag !Numb ~u ~u vity: lved: solve CTD: ~o _Nitri ~o Bottle ~o Bottle ~o Extrac ~o t:Extr Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle ~o !er mb en CTD CTD: d:CTD Seapoin tt te: tt tt tt ted: tt acted: Bottle Bottle Bottle Bottle Bottle Bottle tt ! er ce Volume :Mass t le Bottle le le le Bottle le Bottle le !---- -- -- ------ -------- -------- -------- ----- ------ ----- ------- -------- -- ------ -- ------- -- ------ -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 462 2 2 5.2 16.8203 32.2338 23.4605 60.6 5.75 250.9 0.560 32.2426 0 0.00 0 0.385 0 0.57 0 0.16 6 0.03 0.013 0.000 0.000 0.018 0.000 0.000 0.000 0.000 0.020 0.016 0.000 0.000 0.000 0.067 0.016 0.000 0.000 0.000 0.000 0.004 0.000 0.108 0.000 0.000 0.108 6 461 1 1 27.9 13.5981 32.3275 24.2307 51.2 6.44 280.8 3.208 -99.0000 0 0.37 0 0.454 0 1.69 0 0.67 6 0.25 0.092 0.000 0.005 0.100 0.000 0.000 0.019 0.000 0.083 0.137 0.007 0.010 0.000 0.223 0.032 0.000 0.000 0.019 0.000 0.107 0.000 0.485 0.000 0.009 0.485 6