*2021/10/27 07:47:00.41 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2013/08/24 20:53:26.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 2 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 296244E8 NUMBER OF CHANNELS : 47 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 172 173 2 Bottle_Number n/a 1 2 3 Bottle:Firing_Sequence n/a 1 2 4 Pressure:CTD dbar 5 49.7 5 Temperature:CTD deg_C_(ITS90) 8.12 17.0343 6 Salinity:CTD PSS-78 32.2339 32.503 7 Sigma-t:CTD kg/m^3 23.4109 25.3218 8 Transmissivity:CTD %/m 52.4 58.2 9 Oxygen:Dissolved:CTD:Volume ml/l 5.87 6.74 10 Oxygen:Dissolved:CTD:Mass µmol/kg 256 293.3 11 Salinity:Bottle PSS-78 32.2415 32.2415 12 Flag:Salinity:Bottle n/a 13 Nitrate_plus_Nitrite:Bottle µmol/l 1 11.5 14 Flag:Nitrate_plus_Nitrite:Bottle n/a 15 Phosphate:Bottle µmol/l 0.46 1.09 16 Flag:Phosphate:Bottle n/a 17 Silicate:Bottle µmol/l 2.2 15.5 18 Flag:Silicate:Bottle n/a 19 Chlorophyll:Extracted:Bottle mg/m^3 0.42 0.81 20 Flag:Chlorophyll:Extracted:Bottle n/a 21 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.16 0.41 22 HPLC:Chl-c3:Bottle mg/m^3 0.3E-01 0.11 23 HPLC:Chlide-a:Bottle mg/m^3 0 0 24 HPLC:MgDVP:Bottle mg/m^3 0 0.6E-02 25 HPLC:Chl-c2:Bottle mg/m^3 0.45E-01 0.116 26 HPLC:Chl-c1:Bottle mg/m^3 0.2E-02 0.4E-02 27 HPLC:Me-chlide:Bottle mg/m^3 0 0 28 HPLC:Peri:Bottle mg/m^3 0.7E-02 0.1E-01 29 HPLC:Pheide-a:Bottle mg/m^3 0 0 30 HPLC:But-fuco:Bottle mg/m^3 0.63E-01 0.97E-01 31 HPLC:Fuco:Bottle mg/m^3 0.23E-01 0.142 32 HPLC:Neo:Bottle mg/m^3 0 0.6E-02 33 HPLC:Pras:Bottle mg/m^3 0 0 34 HPLC:Viola:Bottle mg/m^3 0 0 35 HPLC:Hex-fuco:Bottle mg/m^3 0.12 0.192 36 HPLC:Diadino:Bottle mg/m^3 0.12E-01 0.58E-01 37 HPLC:Allo:Bottle mg/m^3 0 0 38 HPLC:Diato:Bottle mg/m^3 0.3E-02 0.11E-01 39 HPLC:Zea:Bottle mg/m^3 0 0.5E-02 40 HPLC:Lut:Bottle mg/m^3 0 0.4E-02 41 HPLC:Chl-b:Bottle mg/m^3 0.27E-01 0.47E-01 42 HPLC:DVChl-a:Bottle mg/m^3 0 0 43 HPLC:Chl-a:Bottle mg/m^3 0.319 0.484 44 HPLC:Phe:Bottle mg/m^3 0 0 45 HPLC:B-Car:Bottle mg/m^3 0.2E-02 0.4E-02 46 HPLC:TChl-a:Bottle mg/m^3 0.319 0.484 47 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 9 F R4 4 12 -99 ' ' 3 NQ C ' ' 13 -99 ' ' 6 F R4 1 14 -99 ' ' 3 NQ C ' ' 15 -99 ' ' 7 F R4 2 16 -99 ' ' 3 NQ C ' ' 17 -99 ' ' 6 F R4 1 18 -99 ' ' 3 NQ C ' ' 19 -99 ' ' 7 F R4 2 20 -99 ' ' 3 NQ C ' ' 21 -99 ' ' 7 F R4 2 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2013-018 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P11 EVENT NUMBER : 26 LATITUDE : 48 55.96000 N ! (deg min) LONGITUDE : 130 10.33000 W ! (deg min) WATER DEPTH : 2755 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0443 LOCATION : Mid-ship ! custom item *COMMENTS Sample Number 172: HPLC:Bottle - %CV > 10 for TChl-a. Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable plastic inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater 58 to 76 days after collection. The duplicate analysis is in file 2013-018_SAL.pdf. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2013-018_OXY.pdf. Based on a precision study run by the dissolved oxygen post-cruise analyst, the titrated values of dissolved oxygen are only reported with 2 decimal places. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For precision and duplicate analysis see file 2013-018_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details on duplicates see file 2013-018_CHL.pdf Flags and comments apply to chlorophyll values only; phaeopigment data are provided for information only and no quality flags are assigned to them. The minimum detectable level for DMS is 0.10 nM, so “0” values should be interpreted as <0.10. For details on the collection and analysis of the DMS data see file 2013-018_DMS_Report.pdf and for duplicates see 2013-018_DMS.pdf. DMSP-D: 20-75 ml of seawater were allowed to flow directly from the Niskin into a magnetic filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For details on analysis see document 2013-018_DMS_Report.pdf. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~5 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. The two Salinity:CTD channels differed by about 0.006 with one reading lower than bottles and the other higher. Delay in analysis likely caused some evaporation of samples, so the two salinity channels are likely reading higher than the comparison suggests. The little information available suggests that there has been little calibration drift in the primary salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. Based on the recommendation from Sea-Bird, the method for calibration of Oxygen:Dissolved:CTD concentration was changed from that used for 2011 and some 2012 cruises. For more information see the Sea-Bird Application Note #64-2 (Sea-Bird_64-2_Jun2012.pdf). For events 0001 to 0039 the Fluorescence:CTD channel was removed due to a malfunction. WARNING: SeaPoint Fluorometer #2228 malfunctioned during the latter half of 2013. There were large values at great depth that got steadily higher through the year until it was removed from service. The data have been left in the file so researchers can see the shape of the profile. Comparison with extracted chlorophyll is recommended. For details on the processing see the report: 2013-018-proc.pdf. $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11--- 12 --13- 14 --15-- 16 --17- 18 --19-- 20 --21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- 47 !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Salinity Fl Nitra Fl Phosph Fl Silic Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis :Bottle ag te_pl ag ate: ag ate: ag phyll: ag Pigmen Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: DVChl- Chl-a: Phe: Car: TChl-a: ag !Numb ~u ~u vity: lved: solve ~o ~Nitr ~o Bottle ~o Bottl ~o Extrac ~o t:Extr Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle : !er mb en CTD CTD: d:CTD tt ite:B tt tt e tt ted: tt acted: Bottle Bottle Bottle Bottle Bottle Bottle HP ! er ce Volume :Mass le ottle le le le Bottle le Bottle LC !---- -- -- ------ -------- -------- -------- ----- ------ ----- -------- -- ----- -- ------ -- ----- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 173 2 2 5.0 17.0343 32.2339 23.4109 52.4 5.87 256.0 32.2415 0 1.0 0 0.46 0 2.2 0 0.42 6 0.16 0.030 0.000 0.000 0.045 0.004 0.000 0.010 0.000 0.063 0.023 0.000 0.000 0.000 0.120 0.058 0.000 0.011 0.000 0.004 0.027 0.000 0.319 0.000 0.004 0.319 6 172 1 1 49.7 8.1200 32.5030 25.3218 58.2 6.74 293.3 -99.0000 0 11.5 0 1.09 0 15.5 0 0.81 6 0.41 0.110 0.000 0.006 0.116 0.002 0.000 0.007 0.000 0.097 0.142 0.006 0.000 0.000 0.192 0.012 0.000 0.003 0.005 0.000 0.047 0.000 0.484 0.000 0.002 0.484 36