*2021/11/08 09:24:06.74 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2013/02/13 18:57:14.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 6 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 6E591B22 NUMBER OF CHANNELS : 48 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 313 318 2 Bottle_Number n/a 1 6 3 Bottle:Firing_Sequence n/a 1 6 4 Pressure:CTD dbar 4.2 2006.1 5 Temperature:CTD deg_C_(ITS90) 1.9106 5.217 6 Salinity:CTD PSS-78 32.5934 34.5883 7 Sigma-t:CTD kg/m^3 25.7693 27.6746 8 Transmissivity:CTD %/m 59.6 66.1 9 Oxygen:Dissolved:CTD:Volume ml/l 1.23 7.21 10 Oxygen:Dissolved:CTD:Mass µmol/kg 53.3 314 11 Fluorescence:CTD:Seapoint mg/m^3 0.84E-01 0.581 12 Salinity:Bottle PSS-78 32.5936 34.587 13 Flag:Salinity:Bottle n/a 14 Nitrate_plus_Nitrite:Bottle µmol/l 16.9 17 15 Flag:Nitrate_plus_Nitrite:Bottle n/a 16 Phosphate:Bottle µmol/l 1.41 1.42 17 Flag:Phosphate:Bottle n/a 18 Silicate:Bottle µmol/l 27 27.1 19 Flag:Silicate:Bottle n/a 20 Chlorophyll:Extracted:Bottle mg/m^3 0.52 0.53 21 Flag:Chlorophyll:Extracted:Bottle n/a 22 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.17 0.17 23 HPLC:Chl-c3:Bottle mg/m^3 0.58E-01 0.68E-01 24 HPLC:Chlide-a:Bottle mg/m^3 0 0 25 HPLC:MgDVP:Bottle mg/m^3 0.7E-02 0.8E-02 26 HPLC:Chl-c2:Bottle mg/m^3 0.65E-01 0.68E-01 27 HPLC:Chl-c1:Bottle mg/m^3 0 0 28 HPLC:Me-chlide:Bottle mg/m^3 0 0.15E-01 29 HPLC:Peri:Bottle mg/m^3 0.15E-01 0.17E-01 30 HPLC:Pheide-a:Bottle mg/m^3 0 0 31 HPLC:But-fuco:Bottle mg/m^3 0.74E-01 0.77E-01 32 HPLC:Fuco:Bottle mg/m^3 0.82E-01 0.83E-01 33 HPLC:Neo:Bottle mg/m^3 0.4E-02 0.5E-02 34 HPLC:Pras:Bottle mg/m^3 0.7E-02 0.7E-02 35 HPLC:Viola:Bottle mg/m^3 0.8E-02 0.8E-02 36 HPLC:Hex-fuco:Bottle mg/m^3 0.137 0.141 37 HPLC:Diadino:Bottle mg/m^3 0.21E-01 0.26E-01 38 HPLC:Allo:Bottle mg/m^3 0.5E-02 0.6E-02 39 HPLC:Diato:Bottle mg/m^3 0.3E-02 0.4E-02 40 HPLC:Zea:Bottle mg/m^3 0 0.3E-02 41 HPLC:Lut:Bottle mg/m^3 0.1E-02 0.3E-02 42 HPLC:Chl-b:Bottle mg/m^3 0.37E-01 0.38E-01 43 HPLC:DVChl-a:Bottle mg/m^3 0 0 44 HPLC:Chl-a:Bottle mg/m^3 0.356 0.412 45 HPLC:Phe:Bottle mg/m^3 0 0 46 HPLC:B-Car:Bottle mg/m^3 0 0.3E-02 47 HPLC:TChl-a:Bottle mg/m^3 0.371 0.412 48 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 9 F R4 4 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 6 F R4 1 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 7 F R4 2 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 6 F R4 1 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 3 NQ C ' ' 22 -99 ' ' 7 F R4 2 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 8 F R4 3 47 -99 ' ' 8 F R4 3 48 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2013-001 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P25 EVENT NUMBER : 58 LATITUDE : 50 0.00000 N ! (deg min) LONGITUDE : 143 36.23000 W ! (deg min) WATER DEPTH : 4125 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0585 LOCATION : Mid-ship ! custom item *COMMENTS Sample Number 317: HPLC:Bottle - CV >10% for TChl-a. Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable plastic inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater within 2 to 21 days after collection. For details on duplicate analysis see file 2013-001_SAL.pdf. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2013-001_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2013-001_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in a controlled temperature lab. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details on duplicates see file 2013-001_CHL.pdf. Phaeopigment data are provided for information only and no quality flags are assigned to them. The minimum detectable level for DMS is 0.10 nM, so “0” values should be interpreted as <0.10. For details on the collection and analysis of the DMS data see file 2013-001_DMS_report.pdf and for duplicates see 2013-001_DMS.pdf. DMSP-D: 20-75 ml of seawater were allowed to flow directly from the Niskin into a magnetic filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For details on analysis see document 2013-001_DMS_Report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogen ion concentration scale was determined photometrically and is reported at 25C and surface pressure. For duplicates and precision of DIC, ALK and pH samples see files 2013-001_DIC.pdf, 2013-001_ALK.pdf, and 2013-001_pH.pdf respectively. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~5 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. The Oxygen:Dissolved:CTD calibration was done using the method described in the Sea-Bird Application Note #64-2 (Sea-Bird_64-2_Feb2010.pdf). For details on the processing see processing report: 2013-001-proc.pdf $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- ---12--- 13 --14- 15 --16-- 17 --18- 19 --20-- 21 --22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- ---46-- ---47-- 48 !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores Salinity Fl Nitra Fl Phosph Fl Silic Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: :Bottle ag te_pl ag ate: ag ate: ag phyll: ag Pigmen Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: DVChl- Chl-a: Phe: Car: TChl-a: ag !Numb ~u ~u vity: lved: solve CTD: ~o ~Nitr ~o Bottle ~o Bottl ~o Extrac ~o t:Extr Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle : !er mb en CTD CTD: d:CTD Seapoin tt ite:B tt tt e tt ted: tt acted: Bottle Bottle Bottle Bottle Bottle Bottle HP ! er ce Volume :Mass t le ottle le le le Bottle le Bottle LC !---- -- -- ------ -------- -------- -------- ----- ------ ----- ------- -------- -- ----- -- ------ -- ----- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 318 6 6 4.2 5.2161 32.5934 25.7693 59.6 7.21 313.8 0.504 32.5936 0 16.9 0 1.42 0 27.1 0 0.52 6 0.17 0.058 0.000 0.008 0.065 0.000 0.000 0.015 0.000 0.077 0.082 0.004 0.007 0.008 0.141 0.026 0.005 0.003 0.003 0.003 0.038 0.000 0.412 0.000 0.003 0.412 6 317 5 4 50.1 5.2067 32.5972 25.7733 60.0 7.19 312.9 0.581 -99.0000 0 17.0 0 1.41 0 27.0 0 0.53 6 0.17 0.068 0.000 0.007 0.068 0.000 0.015 0.017 0.000 0.074 0.083 0.005 0.007 0.008 0.137 0.021 0.006 0.004 0.000 0.001 0.037 0.000 0.356 0.000 0.000 0.371 36 316 4 5 11.2 5.2170 32.5935 25.7693 59.6 7.21 314.0 0.544 32.5940 0 -99.0 0 -99.00 0 -99.0 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 315 3 3 175.9 3.9743 33.7101 26.7884 65.5 2.98 129.5 0.091 33.7104 0 -99.0 0 -99.00 0 -99.0 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 314 2 2 349.8 3.8380 33.9637 27.0039 65.7 1.23 53.3 0.092 33.9628 0 -99.0 0 -99.00 0 -99.0 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0 313 1 1 2006.1 1.9106 34.5883 27.6746 66.1 1.36 59.0 0.084 34.5870 0 -99.0 0 -99.00 0 -99.0 0 -99.00 0 -99.00 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 -99.000 0