*2021/11/08 09:24:04.95 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2013/02/06 03:32:28.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 7 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 82A783D5 NUMBER OF CHANNELS : 12 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 1 7 2 Bottle_Number n/a 1 7 3 Bottle:Firing_Sequence n/a 1 7 4 Pressure:CTD dbar 4.9 215.6 5 Temperature:CTD deg_C_(ITS90) 8.1686 9.0275 6 Salinity:CTD PSS-78 29.3845 31.361 7 Sigma-t:CTD kg/m^3 22.8372 24.2951 8 Transmissivity:CTD %/m 41.3 59.5 9 Oxygen:Dissolved:CTD:Volume ml/l 0.7E-01 4.87 10 Oxygen:Dissolved:CTD:Mass µmol/kg 3.3 212.3 11 Fluorescence:CTD:Seapoint mg/m^3 0.106 0.48 12 PAR:CTD µE/m^2/sec 0 0 $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 7 F ' ' 1 $END *ADMINISTRATION MISSION : 2013-001 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : SI03 EVENT NUMBER : 1 LATITUDE : 48 35.60000 N ! (deg min) LONGITUDE : 123 30.02000 W ! (deg min) WATER DEPTH : 225 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0585 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with disposable plastic inserts and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater within 2 to 21 days after collection. For details on duplicate analysis see file 2013-001_SAL.pdf. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2013-001_OXY.pdf. Nutrient samples were collected in plastic tubes. One set of samples was collected and immediately quick frozen in aluminum blocks stored in -20 freezer. Another set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis using an Astoria analyzer following methods described in IOS Nutrient Methods (1996) Barwell-Clarke and Whitney. For precision and duplicate analysis see file 2013-001_NUT.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -20C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed at IOS in a controlled temperature lab. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details on duplicates see file 2013-001_CHL.pdf. Phaeopigment data are provided for information only and no quality flags are assigned to them. The minimum detectable level for DMS is 0.10 nM, so “0” values should be interpreted as <0.10. For details on the collection and analysis of the DMS data see file 2013-001_DMS_report.pdf and for duplicates see 2013-001_DMS.pdf. DMSP-D: 20-75 ml of seawater were allowed to flow directly from the Niskin into a magnetic filtration funnel containing a 0.7 µm GF/F filter. The first 3.5 ml were collected in a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-T: 3.5 ml of seawater were collected directly from the Niskin into a polypropylene tube containing 50 µl of a 50% sulphuric acid solution. DMSP-D and DMSP-T: Samples were stored in the dark at 4 degrees C for a minimum of 24 hours. They were hydrolized and analyzed later at the Institute of Ocean Sciences. The minimum detectable level is 0.1 nM, so “0” values should be interpreted as <0.1 nM. For details on analysis see document 2013-001_DMS_Report.pdf. Dissolved inorganic carbon (DIC) was determined using coulometric analysis. Total alkalinity (Alk) was determined by potentiometric titration. pH on the total hydrogen ion concentration scale was determined photometrically and is reported at 25C and surface pressure. For duplicates and precision of DIC, ALK and pH samples see files 2013-001_DIC.pdf, 2013-001_ALK.pdf, and 2013-001_pH.pdf respectively. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~5 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peña, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Transmissivity, Fluorescence and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. The Oxygen:Dissolved:CTD calibration was done using the method described in the Sea-Bird Application Note #64-2 (Sea-Bird_64-2_Feb2010.pdf). For details on the processing see processing report: 2013-001-proc.pdf $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- --12-- !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores PAR: !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: CTD !Numb ~u ~u vity: lved: solve CTD: !er mb en CTD CTD: d:CTD Seapoin ! er ce Volume :Mass t !---- -- -- ------ -------- -------- -------- ----- ------ ----- ------- ------ *END OF HEADER 7 7 7 4.9 8.4015 29.3845 22.8372 59.0 4.87 212.3 0.480 0.0 6 6 6 25.3 8.6715 29.7880 23.1143 59.5 4.36 190.3 0.129 0.0 5 5 5 50.2 8.1686 30.0178 23.3661 51.1 4.47 194.9 0.150 0.0 4 4 4 100.5 9.0275 31.0356 24.0372 53.0 0.16 6.8 0.118 0.0 3 3 3 150.1 9.0087 31.3033 24.2494 56.1 0.13 5.5 0.106 0.0 2 2 2 175.1 8.9991 31.3289 24.2709 51.2 0.12 5.1 0.107 0.0 1 1 1 215.6 9.0051 31.3610 24.2951 41.3 0.07 3.3 0.127 0.0