*2021/11/17 11:24:59.88 *IOS HEADER VERSION 2.0 2016/04/28 2016/06/13 IVF16 *FILE START TIME : UTC 2012/08/21 08:46:07.000 TIME INCREMENT : 0 0 0 0.416667E-01 0 ! (day hr min sec ms) NUMBER OF RECORDS : 2 DATA DESCRIPTION : Bottle:Rosette:Up:Stop + CTD:Up FILE TYPE : ASCII CRC : 92B3A5EF NUMBER OF CHANNELS : 46 $TABLE: CHANNELS ! No Name Units Minimum Maximum !--- ----------------------------------- --------------- -------------- -------------- 1 Sample_Number n/a 352 353 2 Bottle_Number n/a 1 2 3 Bottle:Firing_Sequence n/a 1 2 4 Pressure:CTD dbar 5.8 23.8 5 Temperature:CTD deg_C_(ITS90) 11.3975 14.8498 6 Salinity:CTD PSS-78 32.5208 32.5616 7 Sigma-t:CTD kg/m^3 24.1199 24.8324 8 Transmissivity:CTD %/m 52.9 56.4 9 Oxygen:Dissolved:CTD:Volume ml/l 6.07 6.97 10 Oxygen:Dissolved:CTD:Mass µmol/kg 264.7 303.7 11 Fluorescence:CTD:Seapoint mg/m^3 0.836 1.979 12 Nitrate_plus_Nitrite:Bottle µmol/l 6.7 9.2 13 Flag:Nitrate_plus_Nitrite:Bottle n/a 14 Phosphate:Bottle µmol/l 0.84 1.07 15 Flag:Phosphate:Bottle n/a 16 Silicate:Bottle µmol/l 3.8 11.2 17 Flag:Silicate:Bottle n/a 18 Chlorophyll:Extracted:Bottle mg/m^3 0.21 0.4 19 Flag:Chlorophyll:Extracted:Bottle n/a 20 Phaeo-Pigment:Extracted:Bottle mg/m^3 0.6E-01 0.17 21 HPLC:Chl-c3:Bottle mg/m^3 0.24E-01 0.66E-01 22 HPLC:Chlide-a:Bottle mg/m^3 0 0 23 HPLC:MgDVP:Bottle mg/m^3 0 0.5E-02 24 HPLC:Chl-c2:Bottle mg/m^3 0.32E-01 0.76E-01 25 HPLC:Chl-c1:Bottle mg/m^3 0 0 26 HPLC:Me-chlide:Bottle mg/m^3 0 0 27 HPLC:Peri:Bottle mg/m^3 0.19E-01 0.31E-01 28 HPLC:Pheide-a:Bottle mg/m^3 0 0 29 HPLC:But-fuco:Bottle mg/m^3 0.31E-01 0.6E-01 30 HPLC:Fuco:Bottle mg/m^3 0.2E-01 0.68E-01 31 HPLC:Neo:Bottle mg/m^3 0.5E-02 0.1E-01 32 HPLC:Pras:Bottle mg/m^3 0 0 33 HPLC:Viola:Bottle mg/m^3 0.5E-02 0.9E-02 34 HPLC:Hex-fuco:Bottle mg/m^3 0.95E-01 0.219 35 HPLC:Diadino:Bottle mg/m^3 0.33E-01 0.36E-01 36 HPLC:Allo:Bottle mg/m^3 0 0.2E-02 37 HPLC:Diato:Bottle mg/m^3 0.3E-02 0.3E-02 38 HPLC:Zea:Bottle mg/m^3 0 0.6E-02 39 HPLC:Lut:Bottle mg/m^3 0.8E-02 0.14E-01 40 HPLC:Chl-b:Bottle mg/m^3 0.37E-01 0.77E-01 41 HPLC:DVChl-a:Bottle mg/m^3 0 0 42 HPLC:Chl-a:Bottle mg/m^3 0.235 0.409 43 HPLC:Phe:Bottle mg/m^3 0 0 44 HPLC:B-Car:Bottle mg/m^3 0.2E-02 0.4E-02 45 HPLC:TChl-a:Bottle mg/m^3 0.235 0.409 46 Flag:HPLC n/a $END $TABLE: CHANNEL DETAIL ! No Pad Start Width Format Type Decimal_Places !--- ---- ----- ----- ------ ---- -------------- 1 -99 ' ' 5 I I 0 2 -99 ' ' 3 I I 0 3 -99 ' ' 3 I I 0 4 -99 ' ' 7 F ' ' 1 5 -99 ' ' 9 F ' ' 4 6 -99 ' ' 9 F ' ' 4 7 -99 ' ' 9 F R4 4 8 -99 ' ' 6 F ' ' 1 9 -99 ' ' 7 F ' ' 2 10 -99 ' ' 6 F ' ' 1 11 -99 ' ' 8 F ' ' 3 12 -99 ' ' 6 F R4 1 13 -99 ' ' 3 NQ C ' ' 14 -99 ' ' 7 F R4 2 15 -99 ' ' 3 NQ C ' ' 16 -99 ' ' 6 F R4 1 17 -99 ' ' 3 NQ C ' ' 18 -99 ' ' 7 F R4 2 19 -99 ' ' 3 NQ C ' ' 20 -99 ' ' 7 F R4 2 21 -99 ' ' 8 F R4 3 22 -99 ' ' 8 F R4 3 23 -99 ' ' 8 F R4 3 24 -99 ' ' 8 F R4 3 25 -99 ' ' 8 F R4 3 26 -99 ' ' 8 F R4 3 27 -99 ' ' 8 F R4 3 28 -99 ' ' 8 F R4 3 29 -99 ' ' 8 F R4 3 30 -99 ' ' 8 F R4 3 31 -99 ' ' 8 F R4 3 32 -99 ' ' 8 F R4 3 33 -99 ' ' 8 F R4 3 34 -99 ' ' 8 F R4 3 35 -99 ' ' 8 F R4 3 36 -99 ' ' 8 F R4 3 37 -99 ' ' 8 F R4 3 38 -99 ' ' 8 F R4 3 39 -99 ' ' 8 F R4 3 40 -99 ' ' 8 F R4 3 41 -99 ' ' 8 F R4 3 42 -99 ' ' 8 F R4 3 43 -99 ' ' 8 F R4 3 44 -99 ' ' 8 F R4 3 45 -99 ' ' 8 F R4 3 46 -99 ' ' 3 NQ C ' ' $END *ADMINISTRATION MISSION : 2012-013 AGENCY : IOS, Ocean Sciences Division, Sidney, B.C. COUNTRY : Canada PROJECT : Line P SCIENTIST : Robert M. PLATFORM : John P. Tully *LOCATION GEOGRAPHIC AREA : North-East Pacific STATION : P17 EVENT NUMBER : 47 LATITUDE : 49 20.98000 N ! (deg min) LONGITUDE : 135 39.65000 W ! (deg min) WATER DEPTH : 3395 *INSTRUMENT TYPE : Sea-Bird CTD MODEL : SBE-911plus SERIAL NUMBER : 0550 LOCATION : Mid-ship ! custom item *COMMENTS Analysis methods: ----------------- Salinity samples were collected in 200 ml type ll glass bottles with reusable silicone inserts supplied by Xiamen Cuten Accessories Industry Limited and screw caps supplied by Ocean Scientific International Limited. They were analyzed in a temperature-controlled lab on a Guildline 8400B Salinometer (S/N 68572) standardized with IAPSO standard seawater within 1.6 months after collection. The duplicate analysis is in file 2012-013_SAL.pdf. Oxygen samples were analyzed at sea on an automated Winkler titration system (Brinkmann Dosimat model 665 for titrations, Dosimat model 865 for dispensing standards and a PC950 Colorimeter controlled by AutoOxy software V3.4) with modifications based on Carpenter (1965) and adhering to WOCE protocols (Culberson 1991). For details on duplicate analysis see file 2012-013_OXY.pdf. Nutrient samples were collected in plastic tubes and quick frozen in aluminum blocks stored in -20 freezer. One set of samples from 400 dbar and deeper (to be used for silicate analysis) was collected and stored at 4C in the dark. All samples were returned to IOS for analysis. They were analyzed using an Astoria analyzer following methods described in Barwell-Clarke and Whitney (1996). For precision and duplicate details see file 2012-013_NUT.pdf. NOTE: July 2013: A correction has been applied to NIT:Bottle and PHOS:Bottle data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the report: Corrections_to_Nitrate_and_Phosphate_Data_2009-2012.pdf. Chlorophyll samples were filtered onto 25mm GF/F filters and stored in glass scintillation vials at -80C prior to analysis. Samples were extracted in 90% acetone at -20C for 24 hours and analyzed on a Turner 10AU fluorometer which is calibrated annually with commercially pure chlorophyll a (Sigma). Fluorescence readings taken before and after acidification were used to calculate chlorophyll and phaeopigment concentrations (Holm-Hansen et al 1965). Chlorophyll samples were analyzed on board ship in a controlled temperature lab. When duplicate samples were collected the average of two samples is reported. Variability is assessed as the CV% (std dev / mean*100). For details see file 2012-013_CHL.pdf. Phaeopigment data are provided for information only and no quality flags are assigned to them. Note: The variability between replicates was higher than usual and the values are lower than they typically are in comparison to HPLC Chlorophyll:Extracted. The minimum detectable level for DMS is 0.10 nM, so “0” values should be interpreted as <0.10. For details on the collection and analysis of the DMS data see file 2012-013_DMS_report.pdf and for duplicates see 2012-013_DMS.pdf. The minimum detectable level for DMSP in this file is 0.1 nmol/l, so “0” values should be interpreted as <0.10 nmol/l. For details on analysis see document 2012-013_DMS_report.pdf. HPLC samples were filtered onto 47mm GF/F filters and stored at -80C prior to analysis. Samples were extracted in 95% methanol at -20C for 24 hours and analyzed on a WATERS 2695 HPLC separations system as detailed in Nemcek and Pena, 2014. Analysis was performed ~6-7 months after collection. The average of two samples is reported. Variability is assessed as the %CV (std dev/mean*100) for duplicate pairs. All pigments below the limit of detection (LOD) are assigned a zero value. TChl-a is the sum of Chl-a, DVchl-a, Chlide-a and Me-chlide. References: 1. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., and Strickland J.D.H. 1965. Fluorometric Determination of Chlorophyll. J.du Cons. Intl. Pour l’Epl. De la Mer. 30:3-15. 2. Carpenter, J.H. 1965. The Chesapeake Bay Institute Technique for the Winkler Dissolved Oxygen Method. Limmnol. & Oceanogr., 10: 141-143. 3. Culberson, C.H. 1991. Dissolved oxygen. WOCE Hydrographic Programme Operations and Methods (July 1991). 15pp. 4. Barwell-Clarke, J. and Whitney, F. 1996. Institute of Ocean Sciences Nutrient Methods and Analysis. Canadian Technical Report of Hydrography and Ocean Sciences, No. 182, 43 pp. 5. Nemcek, N. and Peńa, M.A. 2014. Institute of Ocean Sciences Protocols for Phytoplankton Pigment Analysis by HPLC. Can. Tech. Rep. Fish. Aquat. Sci. 3117: x + 80 p. Data Processing Notes: ---------------------- Fluorescence, Transmissivity and PAR data are nominal and unedited except that some records were removed in editing temperature and salinity. For details on how the transmissivity calibration parameters were calculated see the document Transmissivity_Calculation.pdf. The Oxygen:Dissolved:CTD calibration was done using the method described in the Sea-Bird Application Note #64-2 (Sea-Bird_64-2_Jun2012.pdf) with a modification allowing a small offset. For further processing details see the processing report 2012-013-proc.pdf. NOTE: July 2013: A correction has been applied to NIT:Bottle data and PHOS:Bottle data from deep-water cruises analyzed at IOS between 2009 and 2012. For details see the report: Corrections_to_Nitrate_and_Phosphate_Data_2009-2012.pdf $REMARKS Quality flags have the following significance: ---------------------------------------------------------------------------------- 0 = Acceptable measurement with no header comment. 1 = Sample for this measurement was collected but not analyzed. Sample lost. 2 = Acceptable measurement with header comment. 3 = Questionable measurement (probably good). 4 = Poor measurement (probably bad). 5 = Measurement not reported (bad). 6 = Mean of replicate measurements. 7 = Manual chromatographic peak measurement. 8 = Irregular digital chromatographic peak integration. 9 = Sample was planned for this measurement from this bottle but was not collected. ----------------------------------------------------------------------------------- $END !-1-- 2- 3- --4--- ---5---- ---6---- ---7---- --8-- --9--- --10- ---11-- --12- 13 --14-- 15 --16- 17 --18-- 19 --20-- ---21-- ---22-- ---23-- ---24-- ---25-- ---26-- ---27-- ---28-- ---29-- ---30-- ---31-- ---32-- ---33-- ---34-- ---35-- ---36-- ---37-- ---38-- ---39-- ---40-- ---41-- ---42-- ---43-- ---44-- ---45-- 46 !Samp Bo Bo Pressu Temperat Salinity Sigma-t: Trans Oxygen Oxyge Fluores Nitra Fl Phosph Fl Silic Fl Chloro Fl Phaeo- HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC: HPLC:B- HPLC: Fl !le_ tt tt re:CTD ure:CTD :CTD CTD missi :Disso n:Dis cence: te_pl ag ate: ag ate: ag phyll: ag Pigmen Chl-c3: Chlide- MgDVP: Chl-c2: Chl-c1: Me- Peri: Pheide- But- Fuco: Neo: Pras: Viola: Hex- Diadino Allo: Diato: Zea: Lut: Chl-b: DVChl- Chl-a: Phe: Car: TChl-a: ag !Numb ~u ~u vity: lved: solve CTD: ~Nitr ~o Bottle ~o Bottl ~o Extrac ~o t:Extr Bottle a: Bottle Bottle Bottle chlide: Bottle a: fuco: Bottle Bottle Bottle Bottle fuco: :Bottle Bottle Bottle Bottle Bottle Bottle a: Bottle Bottle Bottle Bottle : !er mb en CTD CTD: d:CTD Seapoin ite:B tt tt e tt ted: tt acted: Bottle Bottle Bottle Bottle Bottle Bottle HP ! er ce Volume :Mass t ottle le le le Bottle le Bottle LC !---- -- -- ------ -------- -------- -------- ----- ------ ----- ------- ----- -- ------ -- ----- -- ------ -- ------ ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- -- *END OF HEADER 353 2 2 5.8 14.8498 32.5208 24.1199 56.4 6.07 264.7 0.836 6.7 0 0.84 0 3.8 0 0.21 6 0.06 0.024 0.000 0.000 0.032 0.000 0.000 0.019 0.000 0.031 0.020 0.005 0.000 0.005 0.095 0.033 0.000 0.003 0.000 0.008 0.037 0.000 0.235 0.000 0.004 0.235 6 352 1 1 23.8 11.3975 32.5616 24.8324 52.9 6.97 303.7 1.979 9.2 0 1.07 0 11.2 0 0.40 6 0.17 0.066 0.000 0.005 0.076 0.000 0.000 0.031 0.000 0.060 0.068 0.010 0.000 0.009 0.219 0.036 0.002 0.003 0.006 0.014 0.077 0.000 0.409 0.000 0.002 0.409 6